Du Novo GTN tutorial - Make consensus sequences

Annotation: Run this on raw duplex sequencing reads to produce duplex consensus sequences.

StepAnnotation
Step 1: Input dataset
select at runtime
Step 2: Input dataset
select at runtime
Step 3: Du Novo: Make families
Output dataset 'output' from step 1
Output dataset 'output' from step 2
12
5
Step 4: Du Novo: Correct barcodes
Output dataset 'output' from step 3
3
20
2
True
False
Advanced Options:
512
Use default job resource parameters
Step 5: Du Novo: Align families
Output dataset 'output' from step 4
Kalign2
True
False
Step 6: Du Novo: Make consensus reads
Output dataset 'output' from step 5
3
0.7
0
FASTQ
40
25
Sanger (PHRED 0 = "!")
True
False
Use default job resource parameters
Step 7: Sequence Content Trimmer
Paired
Output dataset 'dcs1' from step 6
Output dataset 'dcs2' from step 6
ACGT
0.2
10
True
True
50
Step 8: Sequence Content Trimmer
Paired
Output dataset 'sscs1' from step 6
Output dataset 'sscs2' from step 6
ACGT
0.2
10
True
True
50