FastQCFastQC Report
Sun 30 Nov 2014
Chip-seq_Input_DNA

Summary

[OK] Basic Statistics

Measure Value
Filename Chip-seq_Input_DNA
File type Conventional base calls
Encoding Illumina 1.5
Total Sequences 161531951
Filtered Sequences 0
Sequence length 36
%GC 40

[FAIL] Per base sequence quality

Per base quality graph

[FAIL] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[WARN] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
GGGGG 17540390 6.708389 13.514825 32
CCCCC 7347310 6.185458 11.234757 32
TTTTT 29390610 3.3054726 5.5298185 8
GGGGC 7251345 3.2473602 5.238316 32
TGGGG 10725810 3.211429 4.5194926 32
GCCCC 4439560 3.1919096 5.0972133 32
CTGGG 8774125 3.0761318 3.794928 32

Files created by FastQC

Chip-seq_Input_DNA_fastqc.zip (168.5 KB)
duplication_levels.png (16.3 KB)
fastqc_data.txt (8.9 KB)
fastqc_report.html (7.3 KB)
kmer_profiles.png (57.6 KB)
per_base_gc_content.png (10.4 KB)
per_base_n_content.png (7.8 KB)
per_base_quality.png (8.2 KB)
per_base_sequence_content.png (22.3 KB)
per_sequence_gc_content.png (30.2 KB)
per_sequence_quality.png (16.4 KB)
rgFastQCzbhLhl.log (1.2 KB)
sequence_length_distribution.png (17.4 KB)
summary.txt (520 B)
FastQC documentation and full attribution is here

FastQC was run by Galaxy using the rgenetics rgFastQC wrapper - see http://bitbucket.org/rgenetics for details and licensing