@HWI-H225:69:CBJ27ACXX:8:1101:1098:1921 2:Y:0:AGTCAA
ATTGATNTGAAGAGCGTCGTGAAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGCATCANNNANNNNAAAAAGA
+
)8))**#(0((()(8@555'9),7;8(6;;>5-(-(,((,(597,8&)+538&&)))+###+####+(+++&(
@HWI-H225:69:CBJ27ACXX:8:1101:1269:1930 2:Y:0:AGTCAA
TAAAGAGTGTAGCTCTCGGTGGTCGCCGTATCATTAAATAAAAAAACACAACAGAAACAAAANANTAAACTAA
+
**?DDDG?F89?F=BFFHHG7@@=ABFD8AADCCDACD(55:::3&)((((((++((((+((#+#+(++(+((
@HWI-H225:69:CBJ27ACXX:8:1101:2416:1935 2:Y:0:AGTCAA
TGTAATGTTTGCCTCTGCTCCCGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC
+
6:?BDCD884)/=BBF@;=@E=E<'-';;295;9=;A???;>52?3:8(+8(+++(((4(((4+<@(()0(&&
@HWI-H225:69:CBJ27ACXX:8:1101:3522:1972 2:Y:0:AGTCAA
CGGGATTTAGTTATTCTTTCTTGAAACGGGGTGTGGAGGGCTGAAGCCAAAGGCAGATCGGAAGAGCGTCCCG
+
*1?)):?9**0*0*9*9B@*9BCCFG8(5'-5',;B6=(''('55(5(((,(3(((+(32028&(+()&+&&)
@HWI-H225:69:CBJ27ACXX:8:1101:3731:1973 2:Y:0:AGTCAA
CCCCTTCTGACTCGTTTGGCCCAGGATAGGAAGGGCGTTCTGTGGGATCACGGTGTAAATCACAGTGGTCGCC
+
0'-'(.))..7).-.((,,;66((,(-((;@:91;(00&(((+(39(((((()++)+((+(+(((,((+4&)+
@HWI-H225:69:CBJ27ACXX:8:1101:3543:1985 2:N:0:AGTCAA
TGCTTCAGAAGCTCGGAAGCGCCTCGTGTAGGGCAAGAGACAAAATATCGGAGGGCGCCGGATCATTAAAAAA
+
0099*9909***9*'''-'6':''''.(.(((((,,8(((((((,,(4:0<(&5&&&&&&&&&&+++(+:@49
@HWI-H225:69:CBJ27ACXX:8:1101:4259:1979 2:Y:0:AGTCAA
TGTTGTGTTTTTGTTGGTGTTGGTTGTGTTTTTGTTTTGGTTTTTTTGTCTGTTTGAGATGATCCACCCGCGT
+
5-.)(.(..).('(...((,,((,((',,5+(()+++(+0((+++00)+4:(+44(((((+4++(+(+&)&)5
@HWI-H225:69:CBJ27ACXX:8:1101:4526:1938 2:Y:0:AGTCAA
GCAGTTTATATATATATACTTTTATTCCCGACTTTTTATAAATGCTGAGAACAATTAATTGATACAAATGCTG
+
A@FG;CGEGI47=?AEH33)7?;);C:;>A@>;@3:A<@@5;>;(5>:8>@:?(++(4:@(++>(4>C4((44
@HWI-H225:69:CBJ27ACXX:8:1101:4504:1946 2:N:0:AGTCAA
TCCGCTGGGAACAAGATCGGAAGAGCGGCGTGTGGGGAAATAAAGTAGATCTCGGTGGTCGCCGCATCATTAA
+
'./-''5=5?;?(;>>(6,'((8,99;&--)0)(27&28<(:(((+4+4+(448?20&&45&<5&)&5((:(4
@HWI-H225:69:CBJ27ACXX:8:1101:4617:1969 2:Y:0:AGTCAA
ACTCCTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGTGGCCGTATC
+
**?*009***0?***)/8(()/).'5-'(6)5,(,',(55@B'3;<(2+++(++>(4+22&)))))(+)&&+8
@HWI-H225:69:CBJ27ACXX:8:1101:5356:1988 2:N:0:AGTCAA
AGTTCTCAGCAGGATTTGCAAGCCTGAAACGGTCTTCCTGGTGGACTGGGAGTCAAGGGCAGAGCTAGACTGA
+
=;C*::?<<9B?)09DH>FIIF@FH(=FA@;C;C=EH@@;?;;?=CA=BBDD?C>CACD??&8?CAA>@@CA>
@HWI-H225:69:CBJ27ACXX:8:1101:5746:1998 2:Y:0:AGTCAA
CTGCCTCAAATCAGGAGAGCGTTGTGTAGGGAAAGAGTATAGATTTCTGTTTGCGGGGTATCATTAAAAAAAA
+
*9093?BB*9*.)/).5((6'5(--5;A3;=(;=;-(-((-(,,(;((,,(((,0&&)0&4>:((+4:4:0.0
@HWI-H225:69:CBJ27ACXX:8:1101:6038:1975 2:N:0:AGTCAA
CAGAGATCGGAAGAGCGTCTTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAATAA
+
CBD@BHHGHG1;:(2B@AG';4=C@HB9EBD9;.(.6>AACCC2((5=/588?9>&8?A:>3:@CC@5&)(((
@HWI-H225:69:CBJ27ACXX:8:1101:6362:1939 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
)8?/B<BDH?(5.=@AEEHFED>;@B6==A;;28<>135:@A?BBD5)453?&))8<C::((4@C&5909&95
@HWI-H225:69:CBJ27ACXX:8:1101:7393:1963 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAT
+
A<FDBHIGIHGAE2BA:CD'9A,;=@?<ABDD5@,534@CDD??+<30@>@?<<<A24:A:@CCD@&&))00(
@HWI-H225:69:CBJ27ACXX:8:1101:7732:1945 2:Y:0:AGTCAA
CTATACCAATACTATTTTTTCAGCTTTCCCTTAAGTGAGTAAGATCTGTAGTTTCCATATAACATAATGGCTG
+
8BCCFGGGG;;@CCDGIHHFBBCCECE>CC>C:>@5>CCDDDDCC@C:@@CADDACCC:@:C:::>C4(:8<8
@HWI-H225:69:CBJ27ACXX:8:1101:8831:1968 2:Y:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
<?D4BGH3DG;FDF@AEHIIEGGICB<<;@ACB??B?C3,5:;@A;,>@A?(29&(&0<000<A(4:(44>:<
@HWI-H225:69:CBJ27ACXX:8:1101:9248:1990 2:N:0:AGTCAA
CTTCCGCTCTAGATCAAGGCAAAATCGAATTTTTTTTTGGAGGGGAGGAGAGAAAAGCCCGGCGGGAGCCCGT
+
**0**8(7'())))).==@@((.)).;?))().(9;680&(32>&&&&++(++33((+((&&&)0&)5&+()&
@HWI-H225:69:CBJ27ACXX:8:1101:9655:1976 2:Y:0:AGTCAA
CCAGAAAGCTTCCATTCCAACAAGCTGCAGAAGACAGTATCTGAACAACACCATTGACAGTATTTGCGTTGGT
+
8=).88=ED77A9)7?9AAD)(.66;6.;(5??C5>?(;@;BDA:5(5<<808:>(:3>:++::>(355B<(+
@HWI-H225:69:CBJ27ACXX:8:1101:9924:1980 2:Y:0:AGTCAA
ATCCTAATGTAAACACAGATCTTTAGTTCTGCGTGTAGGGAGGTAAAGAATATGTGGGAGGTTGCCGGTTGTT
+
)11)***0*0*0*00)(0983)))/)))/)))-'-==7);;',,.(((-5;;(-;;(8,,',,(+40&)&&&(
@HWI-H225:69:CBJ27ACXX:8:1101:11025:1976 2:N:0:AGTCAA
TGGCCCAGAGATCGGAAGAGCGTGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAA
+
IEIHGI;FH;@EGGI1?8@>DE/93;=@ACDCB??:<,>@BA:C(::?<+29&+0589)0(+(+>(>CA:)&)
@HWI-H225:69:CBJ27ACXX:8:1101:11961:1942 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAATAAA
+
HF:BDIICI;C/C@AG@EEDD;;;@A9=A@CC:AA@>CC@>@<<2295:-<@<09A8(:@A@ACC>505((+:
@HWI-H225:69:CBJ27ACXX:8:1101:11760:1954 2:Y:0:AGTCAA
TGTAGCGATGGTGTGTTCTGACAAAGATCAGAAAAGCGTCGCGTGGGGAAAAGGAAGAGATATAGGAGGGAGG
+
00/9?*''.-;))((..7))).)).;..;(;(((6(,'((''&))5&&+&2((((((2((,+((4(((0&&)&
@HWI-H225:69:CBJ27ACXX:8:1101:12162:1920 2:Y:0:AGTCAA
TCGGATGGGAACCAGGTAGGGAAAGCGTGGAGATGGGGAAGGGCGCCGAATTAGTAGATNNCNNNNTCAATAA
+
1:):)):)?)*0)(0/9?BF;F.68)5;-'(.))))(,(',('(,',0-)(((++((((##(####(+((+4:
@HWI-H225:69:CBJ27ACXX:8:1101:12267:1985 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGA
+
DBHCFGGHECGGE>CHEGF95?>@CB@BB?>CCCCDDEDCDCBB<<B&4<<@B5982>AD>CDCDB@.055&(
@HWI-H225:69:CBJ27ACXX:8:1101:12566:1992 2:N:0:AGTCAA
ACAGACAGATTGGCTTTATTTATGGACACACTGGAACAGCGGATAGATCGGAAGAGCGTCGTGTCGGGAAAGA
+
2)/)88().777(7?B@)?C37;;(..(,(,,395:((,53;&&)34:A<>(2088350850&+()09&5<<9
@HWI-H225:69:CBJ27ACXX:8:1101:13475:1971 2:N:0:AGTCAA
TTTTTTTTTTTTTTTTTTTTTTAAATCCCCCCTTTTGGGGGTTTTTTTTTTTTTTTTATTTTTTAAAAATTTA
+
6ADCDDDDDDDDDDDBDDDB@<8@((3:ADB558:3(&)8-)5?BDDDB@B50&&&&(+4(+(&+4+((((+(
@HWI-H225:69:CBJ27ACXX:8:1101:13546:1983 2:Y:0:AGTCAA
CTACCTCTGTAGCGCGAGGTGGGAGGAAAGAGGGGAGGTGTCAGTGGGCGGGGTACCCTTAAAGAAAAAAAAT
+
*1***00**0**9)7;'-;'=('((,;>(;5((,',,&((+(+++43(()&&)&&(+(++(+:(4<<38)&)(
@HWI-H225:69:CBJ27ACXX:8:1101:14920:1995 2:Y:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGAAGGGAAAGAGTGTAGAACTCGGTGGTCGCCGTATCATTAAAAAAAAATATA
+
D?6BF@@2;(<4=8'=,5>''((-(8595((9>>CC:A((+4((+8>>:5<80)&8(+4@>CCDD-&)58(((
@HWI-H225:69:CBJ27ACXX:8:1101:15342:1956 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
DHGGEGGEGIEB@68;;@A8@BC@CCE@B?CC3:A@CCCCDD<?<?B<CB9@B59??ADC@@AAC>BB9><)9
@HWI-H225:69:CBJ27ACXX:8:1101:16121:1991 2:Y:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGAGCGTCGTGTAGGGAAAGGGGGGAGATCTCGGTGGTCGCCGTATCATTAA
+
A;;BEEBEFB@?@CE>;;A?;<89A3=?8:5789>AB98(8(+&0&05-3(4:228?B&+0089&*+2(>:4>
@HWI-H225:69:CBJ27ACXX:8:1101:16718:1999 2:Y:0:AGTCAA
ATAGATGGGAACCGCGGCGAGTAGGGAAAGAGTGTAGATTTCGGCGGGCGGCGTATCATTAAAAAAAAATAAA
+
5@@37)).6A)(,3','3)&&&+(383<A((3+(:((++++(+&&))0)0&009-224:3>@:CA9&&0(++4
@HWI-H225:69:CBJ27ACXX:8:1101:17156:1953 2:N:0:AGTCAA
GCCGCTGGGTACAAGATCGGAAGAGCGTTTTTTTTGGAAAGGGTGGAGACCTCGGGGGGCGACGGATCATTAA
+
0**)7(@:5..)=;3))).:1;8?(;>35';(30&0&(++2(<2<&((((((++&09>&&0&&&)&&&(+4((
@HWI-H225:69:CBJ27ACXX:8:1101:17473:1958 2:Y:0:AGTCAA
GTAGATTGGATGCGCGTTGTTTAAAGGAAGAGTGGAGATCTCAGGGCCAGCGGTAACCGGAAAAAAAACATGT
+
)118*?**?***))9'@;'@().)).)(.66?);(6;((->5--(,((((('&))((+&&)9>C@&&&&+(((
@HWI-H225:69:CBJ27ACXX:8:1101:17456:1963 2:N:0:AGTCAA
GGGATTTAGTTATTCTTTATTTAAACGGAGAGGGGAGTCTCGAAGACAAAGGAAGATAGGAAGGGGGAGGTGT
+
;8A63==;7)=A;?=C7;)).)(.66(5?//((',&&+((((+3+)(+3<9((+(+((+(+(+(5&0)&&+++
@HWI-H225:69:CBJ27ACXX:8:1101:17524:1926 2:Y:0:AGTCAA
TTTGGAATGTTCTGGCTCAACTTTGAAGAGCGTCGTGTGGGGGAAGAAAGGAAAATTCGGTTNTNGCCTTTTT
+
:DG*0****00*0**9D***.=)))))..(8;'-'5'6((''','(2((((((+((4(28?&#+#+((((+:(
@HWI-H225:69:CBJ27ACXX:8:1101:18210:1981 2:Y:0:AGTCAA
TCCGTTGCGTAAAAAATCGGAAGAGCGTAGAGTAGGGTGCCAGGGACGATTTTAGAGGTCGGCGGTTCATTTA
+
'=4().5','((.((,(5(,55'(8(&)85(((((+8(((((,(&(+)&++(((((((+353&0&&&(+(((+
@HWI-H225:69:CBJ27ACXX:8:1101:19313:1944 2:Y:0:AGTCAA
CTGACTCAGATCGGAAGAGCGTCGTGTAGGGGAAAGGGGGAGATCTCGGAGGGCGCCGGATCATTAAAAAAAA
+
<BFCG@BFHB9B;?1C'=28@-5:'5?BBED'',,(;('')&)(++((+&)5&05059&5&)<@A>@@CDDD9
@HWI-H225:69:CBJ27ACXX:8:1101:20038:1961 2:Y:0:AGTCAA
CATTTGTCTTGTTGACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG
+
@4=FG=F9CC9=)..7?C;C@6;7;A??AA3>(;?(,,((())2909(3(:?+222(++4((4:@A@A((+(0
@HWI-H225:69:CBJ27ACXX:8:1101:20986:1987 2:Y:0:AGTCAA
CGTTCTTTGGGGTTTTTTTTGGTTTTTCATACCGGGGTTTTCCTCTCCGCTCCCGGTTTCCTGGGCATCAATT
+
*:?:))*0*?@(777(78:'''.(5;(5((-,(','&)02(((((((+)-&)&()&0&&+4+(+((((+((+>
@HWI-H225:69:CBJ27ACXX:8:1101:1083:2118 2:Y:0:AGTCAA
GACTTGTTGGCCCAGAGATCAGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATGTCCGGGGTNGNCGTATCATT
+
((088.8B(6=@FH=(.=?>).).7?>;='5>8853>C8&)0(22(&(+2(4(43(2&0-0#(#(((+++(+>
@HWI-H225:69:CBJ27ACXX:8:1101:1188:2177 2:Y:0:AGTCAA
TTATTTCTTCCGCCCCGCGTTGAGTAAAGGTTAACACATGTAGAAAGGAGGCGTCTCCGTTAAAAAAAAGCGG
+
0?)*****0*07(-''-'5,''(((---(-,((,(5(,(,5;@4((4(++&(&)&&++&+(+(((+59&&(&0
@HWI-H225:69:CBJ27ACXX:8:1101:1480:2125 2:N:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCCGTATCATTAAAAAAAAAATAA
+
?DH@GIGIIGHJ(;8=:?B>/5;>CBCDDDA?,:ADCC(4:@@D8?&)409@305ADDEEDDDDDB<99&((4
@HWI-H225:69:CBJ27ACXX:8:1101:1510:2005 2:Y:0:AGTCAA
ATACATCGGAAGAGGGTCGTGCAGGGAAAGAGTGAAGATCTCGGTGGTCGTCGGATCACTAAAAAAAAAAAAA
+
):@)*00?6((<(.(-;C/-'(.)69AAB@((-;(;(;:@;:(58<;&+-)2)05?>8(++9(4><505<&0&
@HWI-H225:69:CBJ27ACXX:8:1101:1506:2024 2:Y:0:AGTCAA
TCTAGCGTTGGTGTGTTCTGCCTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGC
+
?)0/9DEAFEG5;.@=DACA?CE@BDFFEDDCDBB???;?609(+:CD3(<<(9(0(384::A:87(29&4&)
@HWI-H225:69:CBJ27ACXX:8:1101:1519:2097 2:Y:0:AGTCAA
GGTATTGCTGCTTCCTGTGACAACAGATCGGAAGAGCGCCGTGTAGGGAAAGAGAGTAGATCTCGGTGGGCGC
+
):0??<?FHC**9=<.=8==@GGF=@7@E2:;?'(6(''3/',,,::((2<A(3((+4(+((+4((285&()&
@HWI-H225:69:CBJ27ACXX:8:1101:1645:2184 2:Y:0:AGTCAA
ATTTCTCGGATGAGTGTCGTGTAGGGACAGGGTGTACATCTCGGTGGTCGGCGTATCATTTAAAAAAAAACAG
+
)?))*0*))0(-8))./8-5'.)))(=(5;(((,,6(--((((,5(''5'&005&2++(:(444:@@BBB&89
@HWI-H225:69:CBJ27ACXX:8:1101:1769:2201 2:Y:0:AGTCAA
GGGATTTAGTTATTCTTTATTGAAACGGAGTGTGGAGTGCTGAAGACAAAGGCAGATCGGAAGAGCGTCGTGT
+
'''(7BDCEE9=?EBBCE@DF;>CC>AB7?(5:@?AD@AA@AAC@>ACDDC<8<28>>A@B?0889<)<>&58
@HWI-H225:69:CBJ27ACXX:8:1101:1889:2249 2:N:0:AGTCAA
CCAGAAAGCTTCCATTCCAACAAGCTGCAGAAGACAGTATCTGAACAACACCATTGACAGTATTTGCGTTTGT
+
GGGCHCH;).=7C3?E>AEBB5;;?(6;AACCC?5?<5;>3@:@C5(5+(28<AC@::(++::@C:@39@&8(
@HWI-H225:69:CBJ27ACXX:8:1101:2104:2006 2:N:0:AGTCAA
TGCTCCAGAAGATCTGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAA
+
HGGIGIIE<F<B*00?BF@B98<<CHHH=7AHD3;?;3>(.6>;@C:>;AB88=@AB08@&000?>>4>:@A>
@HWI-H225:69:CBJ27ACXX:8:1101:2012:2014 2:N:0:AGTCAA
CTTCCCCTCTGACTCCAGTCGACATCGAGGAAAGTGTGGTGATTTTTCCTGGCGGCGGCTCGCCGGGGAAAAA
+
41:4B3)08)0/8??*0.))-(''')(.;''(..).(((.(((-(((,((,5,'0&00&&)&&&&&&&&)((+
@HWI-H225:69:CBJ27ACXX:8:1101:2222:2129 2:Y:0:AGTCAA
GCTGCTGGGGACAAAATTGTAAGGGGGTCCTGAAGGGAATAAGTATCGATATTGGCAGCATTCGTATCTTTTC
+
F:*****0))86BF)8)))/)//)8''5'(...(5;8(9((((,(,5((+(&(+:((((+(+4((39?(4+((
@HWI-H225:69:CBJ27ACXX:8:1101:2475:2017 2:N:0:AGTCAA
TCTTGGCCCGGGGGTCGGAAGCGCGTCGGGCAGGGAAAGAGTGTACCTCTCGGGGGGCGGGGGACCTTTTAAA
+
19?:?D??F)7(5,9/'3,(,(&)05985<&&&)08?B<3(+4:4+4((+()(&&)&&&&&095&8(+>(4>(
@HWI-H225:69:CBJ27ACXX:8:1101:2453:2022 2:N:0:AGTCAA
TCGGAAGAGCGTCGGGTAGGGCAAGAGTGTAGATCTCGGTGGTCGCAGTATCATTAAAAAAAAAAAAAAATTA
+
:??FEGGGGIIF?A@@F8DAA.5;?;@?;;@@ADCEDD7@BDDDBB9>@C@CCDD:@CDDD990&&0&)&(((
@HWI-H225:69:CBJ27ACXX:8:1101:2423:2022 2:Y:0:AGTCAA
GCTGCGGGGAGGGGATTCGGATCCGGGCTCTTCCCGGGTCTCTCCCCGATACAGATCGGAACAGCGAGGTGCC
+
G@-''-:47',0&&))+(2++()+(00&&(((++35-&)&(+((++5<&0&&((4>(+)((&+(()&)&&(((
@HWI-H225:69:CBJ27ACXX:8:1101:2294:2052 2:Y:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGGATCATTAAAAAA
+
BB:9?FFGHCHG9BBFBGH;CB8-5@=4.;@DDECCB95(3(-,,53>CAB(2-&(9-00&055(4:>@AC><
@HWI-H225:69:CBJ27ACXX:8:1101:2365:2077 2:N:0:AGTCAA
GGGGGGGAAAACGCGGTTTTGTTATTGTTACGAAGTAAATGATTCGTATGCTGTACATAGCTGTTATAGAAGT
+
@GB<EDD7BD?C30<@8@BBA?<>CC>CC@CBDBD?CD>C>>@CBBC<AACC@@>:@>>CDCAAC:ACACD@:
@HWI-H225:69:CBJ27ACXX:8:1101:2545:2243 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGATCGCCGTATCATTAAAAAAAAAAAAA
+
6??3BF?@D;('(.)--1=BB=@;;;3>A@C3(5-5:;35;A91'5&&+)+95))8(:>C:>:@409@9<9)5
@HWI-H225:69:CBJ27ACXX:8:1101:2855:2096 2:Y:0:AGTCAA
TCTGGGGTGTTCTGACTCAGCACGGAAGCCCGTCGGGTAGGGAAAAAGGGTAGATTTGGGAGGCCGCCGTATT
+
F*:4?)?07(7=)/=)/78BF)6''-;'(.(3,,322,85<BC(8(8&((+(((((+(49(80(2&09&))23
@HWI-H225:69:CBJ27ACXX:8:1101:3061:2045 2:Y:0:AGTCAA
AAGCTCAGCAAACTTGCATGCTATGTGGGCTGTGTGACAATCCAGACCAGGAGCGTAGCCAGCACTGATTTGG
+
CGC4@G@FHEHGHCEHHGHFCDFD7BBCBDE8;;A2>CC>AA?A>C88A8<??(5<?<BDCB<A?AC>ACDD@
@HWI-H225:69:CBJ27ACXX:8:1101:3147:2230 2:Y:0:AGTCAA
GTAGATCGGAAGCGCGTCGTGTAGGGAAAGAGCGTAGATCTCGGTGGTTTCCGTATCTTTAAAAAAAAAAAAT
+
):@1B@9807D@26@8A'=',(,5;?>AB(>(('8?-<C(+((+22&0((+4(>2+8((4>4:4:)5@55<&+
@HWI-H225:69:CBJ27ACXX:8:1101:3513:2025 2:Y:0:AGTCAA
GGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
0C;FEHJGIJGF>F=G<BF9;C;@GFDF66;CDEB<A?C5@,;3>A3;>?B8<B5@00<955A8:4@4>@CCB
@HWI-H225:69:CBJ27ACXX:8:1101:3634:2087 2:Y:0:AGTCAA
GGCGGGCAGACTAGGTTTTTCTATTTTTAGGAAGGAAATGTTTTGGGTGCGGTAAATAGGTGTTATAGAAGTG
+
)))0''''',;-;>(5((,(,(5,,(,((((3(((+((+4((+++-&2&(&&)&(+((((+(+23@(4(:>+(
@HWI-H225:69:CBJ27ACXX:8:1101:3655:2089 2:N:0:AGTCAA
GGGATTTAGTTTTTTTTTATTTAAACGGAGGGTGGAGGGCTGAAAACAAAGGCAGATCGGAAGAGGGTCGTGT
+
;'-(7=D47@C)7?'9?B9@A((5:(28(0&)2<B08()&(&2:(+(2<A93((++++8&2<&+2+02(0&0(
@HWI-H225:69:CBJ27ACXX:8:1101:3618:2202 2:N:0:AGTCAA
TGCTCTGCCCCAGAAGATCGGAAGAGCGTCGTGTATGGCAAAAGGGGAGATCTCGGTGGTCCCCCTATCATTA
+
4'777).=).;,((6;3@A;'5==((,,'+500(((+9(9A((+(2&))&3:@>(8++5&+(22&0(44((>(
@HWI-H225:69:CBJ27ACXX:8:1101:3834:2093 2:Y:0:AGTCAA
CATAGTTCATTTGTCTTGTTGCCTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC
+
C;@CCHJGI;@G4C;?E9AE;.7?D@CE>AECDBDDBDDCAB?B><(+0)8555((4>?(8??33(++4:4(4
@HWI-H225:69:CBJ27ACXX:8:1101:3928:2192 2:Y:0:AGTCAA
GCTGCTGGGAACAACGTCGGGAAAGGGACGTGTTTGGACACGGGGGGGCACCGGTTAGTTGACGAATTATTAA
+
1**119*9B3B100(08DF''-'(6(-((''(.((((((((',')&0-&*+(&)&&&(+((((&()((++4:(
@HWI-H225:69:CBJ27ACXX:8:1101:3979:2196 2:N:0:AGTCAA
GTTGGCCCAAAACTCGGAAGGGCGGCGGGTAGGGAAAGGGAGTAAATTACGTTGGCGGCCGTTTCATTAAAAA
+
AFC26@CC5(((;7?;=61',3('&)0&00082((+++(++&(+(((+((+(++2(&)509&&+4(:(+(4+4
@HWI-H225:69:CBJ27ACXX:8:1101:4394:2028 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGAAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATAG
+
?:@8?FFH';5;(6);5;?BC(,;@BD2;<>9:::;55((,,8?88504-)2-50?2>@>(:>A@))0&&+4(
@HWI-H225:69:CBJ27ACXX:8:1101:4450:2079 2:Y:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGGAAGAGTGGAGAAGTCGGGGGTCGGGGAATCATTTAAAAAAAGGTGG
+
(90BF9)=E5@E(:'',33,',5((2&&5<<0,3(:((((((()&&00)0)&)&0(((4:(4:A:4&&&(+(2
@HWI-H225:69:CBJ27ACXX:8:1101:4864:2126 2:Y:0:AGTCAA
GCCGCTGGGAACCAGATCGGCCGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCCGTATCATTTA
+
@CF</;AEFFFF1>ABCABB//'89BD&2&&0+(:383(8(33(+4(+4((448?2<5&+5<B5&)2<>:>(+
@HWI-H225:69:CBJ27ACXX:8:1101:4841:2246 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATAA
+
AHGIGIEHG7FGAAGH@DEB/(6;>?>ABB>C>AC(:A(:@:?@??<0409<@B<B?@@D:>@C@@&&))2(+
@HWI-H225:69:CBJ27ACXX:8:1101:5114:2220 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAAAGCGTCGTGTAGGGAAAGAGTTTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
*::9D*?D?B39*00(7B')8@85;E5A;)7;5ABD(-5((-5(:(5,>2,(,9)+502))&+(+:@::44>>
@HWI-H225:69:CBJ27ACXX:8:1101:5200:2225 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
@@F9FIIJDECE;CCBGAEFBBCCCC@BB?CC5>:@>>;:@:<B<<5&4)9<@05A8@@C:>CCC955&))50
@HWI-H225:69:CBJ27ACXX:8:1101:5482:2064 2:Y:0:AGTCAA
GTTCCCCAGAGCGGCCAGGCGGCATTGATGGGAAGGTTCGAAGCATCGCCGGGCCCCCGGGCTTTAAAAACAA
+
))0:?*0)0((*<'''-.(2'',''(,5(((((((+(+(&+((&(+(&(&559&&&)0&&&&)(+(((+4&+(
@HWI-H225:69:CBJ27ACXX:8:1101:5410:2080 2:Y:0:AGTCAA
CCACAAGTTTTCATTCCAAAAAAGTGCAACAGTCAGTTTCTGAAAAAAACCATTTAAAAAATTTGGCGTTGGT
+
11*)1:)**:*9<9?<B*?*??''/))84).()...7)7)))).));',,8((,(((,(()+4>(((&)&)((
@HWI-H225:69:CBJ27ACXX:8:1101:5545:2102 2:Y:0:AGTCAA
GCCTCTGCTCCAGAAGATCGGAAGAGCTCCGTTTAGGGAAATGGTTCAGAAATCGGTGGTCGCCGCATCATTC
+
BG4)8=)7=))).7=)=7)(,,,'((,((-3,5:9:A(5<8(((+((+44+(4(()+895>&&+&&)5&+:+(
@HWI-H225:69:CBJ27ACXX:8:1101:5631:2204 2:Y:0:AGTCAA
ACCCCTTGGCCCAAAGACCGGCAGCGCGTCGTGTTTGGCAAGAGCGGAGGTCCCGGGGGGTCCCCCAAAATTA
+
***))0:)*:30)00)*/)';/;A19'33,;,8(((+((+42+((0&&&)++(205&&)&)&++(&&((23(+
@HWI-H225:69:CBJ27ACXX:8:1101:5798:2020 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATAA
+
B?DEHEE=AEF8(8FG@@BBB?>@CCCBDB>C(::>@>C:@AABBBD0:@>B995A<AAD:ADDC@9@<)+++
@HWI-H225:69:CBJ27ACXX:8:1101:5962:2026 2:Y:0:AGTCAA
TAGCTCGGAAGAGCTTCGTGAAGGGAAAGAAAGGAAAAATCGGTGGTGGCCGGATCATTAAAAAATAAGGAAA
+
F<***07@AB:BF>7))-=;)-.7,(6.((((6,;3((,(:(3,5&&((+()&)&5?::@C>A@-(:@:::>>
@HWI-H225:69:CBJ27ACXX:8:1101:5932:2138 2:Y:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTTAAAAA
+
HIIJBBBF9DEHAHIIEGHGIDIBFHB<ACBEF@>AA;?(;(>@>A(;>A78?B&4&0<9&)22@@A(+4>:@
@HWI-H225:69:CBJ27ACXX:8:1101:6084:2119 2:N:0:AGTCAA
CAGAAAGCTTCCCTTCCAACCCGCTGCAGAAGACAGTATCTGAACAACACCATTTACAGTATTTGCGTTGGTG
+
GGH3=F383==C7=DCE;=='',3>3;;;;55>::<@@5;B5:>:((++<?8:>(4:44(+4:>>C75<.(+2
@HWI-H225:69:CBJ27ACXX:8:1101:6364:2172 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAATTAA
+
@FGIJIJIG?GHFEEH<EHF>(6;>?;ABACC-:(>A@@C>C?D?<00:05?-&0<<@CD:4>A@5559(((+
@HWI-H225:69:CBJ27ACXX:8:1101:6652:2015 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
?AAC>DBGE1CHGIJG@CE58(.6@C>AB;>A(;(55;35>>AB(895459@>00<A@@C4:CCCB>&00.0<
@HWI-H225:69:CBJ27ACXX:8:1101:6538:2112 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAATAAAAAA
+
>)?*0?*?0-7;FG@E5CA'96=?=@(;99:9>@C>A@CCC:8?8<<)>5>@9)0((44:((:C:&(((+(+9
@HWI-H225:69:CBJ27ACXX:8:1101:6779:2193 2:Y:0:AGTCAA
GACTTGTTGGCCAAGAGATCGGAAGGGCGACGTGTAGGGAAAAAATGTAGATGTCGGTGGTCGCCGTATCAAT
+
8*:B?:B<?)*0*(0/(8=).5(-'(-(-''3,,,,:(8((9(5&(+44:44(+((0)(004008&09+(((+
@HWI-H225:69:CBJ27ACXX:8:1101:7182:2145 2:Y:0:AGTCAA
AAACTGGGGAATTGAGACCATTGATGCTAAGGGATATTAAGGAATAGCAATTGCTGGTTCCTGTGAAAAAAAA
+
()))/)7@;?/2.7)7(.((6.(((-(;>5((,((,;5((5>@A:((((++44(4((+444:(+4((+:(0&&
@HWI-H225:69:CBJ27ACXX:8:1101:7198:2163 2:Y:0:AGTCAA
AAATTGGGGACTTGAGTCCATTGATGCTAAGAGATATTAAGGAATAGAGATTGCTGCTTTCTGTGAACACAGA
+
(87).)7=65'.));?;;@(..(6.;>>(-;5;((,5;>C(:@?C<(:@>AC3+4:(+:(::3:>3>3:+2+9
@HWI-H225:69:CBJ27ACXX:8:1101:7419:2102 2:N:0:AGTCAA
GACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT
+
3?DDHAHI)??FF@FFGEG@HHHHDHCEBDEB>>;ACDD>??55((5(:4:A3@A<?28@0()-80&0<<@CA
@HWI-H225:69:CBJ27ACXX:8:1101:7250:2148 2:N:0:AGTCAA
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAAAAAAAAACATC
+
>1?FHIGGCGHGABH/6)=DHEAEEDE.?7?>CDE>>@7(5;8@=;(5&05-8>A4:>C@0)&)&0&)&&(((
@HWI-H225:69:CBJ27ACXX:8:1101:7662:2006 2:Y:0:AGTCAA
ACAGACAGATTGGCTTTATTCATGGACACACTGGAACAGCGGATAAATAGGAAGAGCGGCGTGTAGGGAAAGA
+
11C3C)1)0:BGG*9?@*??<?4B@G33=FFH3@DG>G)=<</',(-((5;>@(5(5;'00&&)((:<(22((
@HWI-H225:69:CBJ27ACXX:8:1101:7645:2057 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
GGE9:?FGIIBD9?FGG;<=CFGE@@:B(;BDF>AB@>C((-;@CC:>A<7(88&()5?95&(2+4>:>@AC9
@HWI-H225:69:CBJ27ACXX:8:1101:7757:2134 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
IJIGIGIJJIIJJJJIAGH?DB>CEDCBDBDD:@ADDDECDDDD?@@9ABDD>5@B?ACD:ACDDD@@55000
@HWI-H225:69:CBJ27ACXX:8:1101:8095:2011 2:Y:0:AGTCAA
TTTGCGCCAGGAGTCCGGAAACGTCTTGGAGGGGAAAGGGGTGTTTATCCGGGGGGGCCCGAGCAATAAAAAA
+
;-''.','',,,5,(((')00(((+(((((,8@&&08((())&&((++4((&&&005&&&&&)&)(:(+(::)
@HWI-H225:69:CBJ27ACXX:8:1101:8460:2057 2:Y:0:AGTCAA
GTGTTCCTGTTAGGAAAAGCGTGGAGAAGGGAAAGAGGGTAGATTTCGGTGGTCTCCGTGTTATTAAAAAAAA
+
6.)))))...=)).7=77)(,(',(((,,9/(,(,(+(&(((44(+(9-(2&)4(:(02(22(+:(++::99&
@HWI-H225:69:CBJ27ACXX:8:1101:8669:2195 2:Y:0:AGTCAA
TCCTCCCAAAACTATTTTTTGAGCTTTCCCTTAAGAGAGTAAAATTTGTAGTTTCCATTTTACATAAAAACTT
+
;)))..().((..;))(.('',,(,,:(5((,5>3(498(::((+++++(++4:(4+((((+((++:(+(&((
@HWI-H225:69:CBJ27ACXX:8:1101:8652:2205 2:Y:0:AGTCAA
CTATACCAATACTATTTTTTCAGCTTTCCCTTAAGTGAGTAAGATCTGTAGTTTCCATATAACATAATGGCTG
+
?*00?B>??D>DB8DFHHE@@FFACEH>=7A>3=7.?B@;;@@>;;36;(;>AD@(5-55>C3>(:>5;><A7
@HWI-H225:69:CBJ27ACXX:8:1101:8925:2000 2:Y:0:AGTCAA
TCCTTCGATGTGACTTGTTTGGCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAAAGGGGAGATCCCGGTGGTC
+
*?*11*)0:*0*00*0?/9)06)83(6=)==C65(-(;),,;8>@((((5:9?(+)(2&)))34(3+&0&50(
@HWI-H225:69:CBJ27ACXX:8:1101:8882:2183 2:N:0:AGTCAA
AGGTTCAATGCCCCCATTTGAGGGCTTTTTTTATTGTCATCATTATTAAACATGCTAAAGTCACATACAGTGG
+
BDGHHEHHGGIIIJJGIJHBECEFFFDEEEDD?CD5@;>CACCC:@CCDA>>@>ADCCD3::ACCCDDD:4>C
@HWI-H225:69:CBJ27ACXX:8:1101:8978:2250 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTACGGAAAGAGCGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGA
+
07?9*8=B:5@/(=D84(5,'..((3;;;?C?(8'+0?>(:@??+29545)2&))8<>::+>>C395&&&))+
@HWI-H225:69:CBJ27ACXX:8:1101:9233:2010 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGTATCCTTAAAAAA
+
CA?B?D3?BDAFGB8.??;((.5BG85-(7;>B96.6@?(9=>(5;@A555<25&,5<@5&&2++(4>>3>3)
@HWI-H225:69:CBJ27ACXX:8:1101:9233:2028 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
JJJJIJIGJJJJIJJJGJIIJIFHGHAEADFFFCDDCED5@CCCDCCACDDBDD9CBBB555AA:>D@CCDDD
@HWI-H225:69:CBJ27ACXX:8:1101:9172:2096 2:N:0:AGTCAA
CAGTTGCCATGATTTTCTCCTTGATCAAGTTGAGAACTCAACGGAAAGGGAACGCTTGGTACTTGTTGGCCCA
+
0**9**/9*?@DHHEG)87@FE9C>@EH:?A))).7.;@@C>6;=@;5<@29,89<?((+(+>@(4:((++88
@HWI-H225:69:CBJ27ACXX:8:1101:9215:2187 2:Y:0:AGTCAA
AAGCTTGACTACCCTAAGTTCAACTACAAATGGACCTTGAGAGCTTGTTTGTAGGTTCTAGCAGGGGAGCGCA
+
9+AC@998<9CB@>*0):**9**0??@B:D443E3;FH;8=2)=@D3A3?=().).7;@A;@3.;89;22235
@HWI-H225:69:CBJ27ACXX:8:1101:9440:2009 2:Y:0:AGTCAA
GGGTTTTAGGTATTCTTTATTAAAACGGATTTTGGTGTGCTGAAGACAAAGGCAGATCGGAAGAGCGTCGTGT
+
'4'(5;CE)).77;).;C66>(;@;;?=(',(5(3(,22++(+((++((339(<?<@(()28-((30&+)&0(
@HWI-H225:69:CBJ27ACXX:8:1101:9496:2176 2:N:0:AGTCAA
CCTTTGCCTCTCTGCCTCTGCCTTTCTCTCTTAGCCTCTGTTCTCTACCACACTGCCCCTCTTCCCCTCTGCC
+
.))..)).)).)7))7))7;3;);@3.(63;@(--;3(((,;(5,5(,((4<<<(4?A?<3+(4>9?()(((:
@HWI-H225:69:CBJ27ACXX:8:1101:9883:2040 2:N:0:AGTCAA
GACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCATT
+
'-7@D3@G2=)7=?B=?@9;AB??:2<A?@B@)5?(4>@>B??99+4:@@>>AD>A<(890(00<-&)5&(4:
@HWI-H225:69:CBJ27ACXX:8:1101:9915:2204 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAACAA
+
?BFCHGEHHIIIEHBG';B9/@;@>A9AB?CCCCACC@C>@@AB8<<0@3<?>-5A?:>C:@CAC@D9550++
@HWI-H225:69:CBJ27ACXX:8:1101:10089:2046 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGTCTCATTAAAAAAAATCGTT
+
)06?B4BFHAHA;@=@@;''5;.;@=3((;;;(3<(8(;>;AAB<?<>C&)<-&&23::4+:A4@-&&+((2+
@HWI-H225:69:CBJ27ACXX:8:1101:10144:2181 2:Y:0:AGTCAA
AAATCATAGTTTTCATGGGCCAGAAATCCAGGTGCAGCCTGGCTGGATCCGCAGGTTGGACGTTGTAACTAAA
+
EEGGIJGIICEHIC>EEFHFFFFBCDDDACCDDCCCCDDCB@BDDDBDDD?B9@BBCCDCCDDB<8>CCCCCD
@HWI-H225:69:CBJ27ACXX:8:1101:10284:2123 2:Y:0:AGTCAA
CTTGTCTTTGCCGCTGGGAGAAAGATCGAAAGAGCGGGGGGTGGGGAAAGGGTGAAAATTTCGGGGGGCCCCG
+
9F))/).74=@=5'549A((',;5;((5(5,'((()&&&&))&093(&((((+(((++((+(30&)9.&&))&
@HWI-H225:69:CBJ27ACXX:8:1101:10466:2145 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
DG@GGCA=EDHGE>CHIJG496;>AB>AB?CC5>>55;;>C@<82<<&>5<B<&&8((4>(4@@CBD9.5&&)
@HWI-H225:69:CBJ27ACXX:8:1101:10381:2157 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
IGIGHGIJIJGBFHIJJIH?DD@;ABABDBDC>ACDACDCDDDDBDB0:<9?<55A<>AD>CDDCDD<.55&0
@HWI-H225:69:CBJ27ACXX:8:1101:10489:2174 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
GEADGGGGIGHE2;7A@;=DBBCACC6;?A;A:>CDCDA:@(518<35C>>?9)5?ADCC::>CC@@00&&)0
@HWI-H225:69:CBJ27ACXX:8:1101:10355:2198 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATCT
+
GDGGGGGGIGIGGHIGACE?>?;@CB>A2=?C>ACDCDECDD?82<B)45@B@5-8?@CDCCDCCD>&))(+(
@HWI-H225:69:CBJ27ACXX:8:1101:10548:2104 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGGCGTGCAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
D)?GGH<FB@F34/8FG<;D@9='53,8'(,9;?<<8((4>4+43(+:@?0870&4)52&5&+<@>>>>A3>5
@HWI-H225:69:CBJ27ACXX:8:1101:10602:2181 2:N:0:AGTCAA
TCCCCCGGGAACCAGATCGGACGGGCTTTTTTTTTGGCACGCGTGTCGATATCGGTGGTCGCCGGATCATTAA
+
*94*0('-''-5-=A()..';'3',,&8((3&5&&&&++())0&)&+&22()4<B+(50()&8&&&&0833(4
@HWI-H225:69:CBJ27ACXX:8:1101:10619:2191 2:Y:0:AGTCAA
CCCGGCGCGACCACGACCGGAAGGGCGTCGTGTGGGGAAAGAGGGGAGAAATCGGGGGGGGCCGGATCAGTGA
+
1?D?))('-''-'(,',9/385&(&+&&)&)&+(+70+&+2(,(0&)&)+(++20&&0)&))&)&&&)+(4(+
@HWI-H225:69:CBJ27ACXX:8:1101:11033:2110 2:N:0:AGTCAA
TTTTTCCCATTCCTTTTTTTCCCCTTTTCCTTTTTTGTGTAAAATTTTTTGTTTTTTTTTAACATTATTTCTT
+
.'5;'.7))))((((.>CB;5((,,+3(3:+33((0&((+(++((((+>&&23?<(&)&0+3((4(((+(+((
@HWI-H225:69:CBJ27ACXX:8:1101:11435:2027 2:Y:0:AGTCAA
TGCCGCTGGGAACAATGACGCAGGCATTTCCACTTGGCTAATATACACATTTTAGGGGGGCGACGGATTATTA
+
;A@)'-'-:?;)(66(.(.5'''',(((53(((++3((+((+((((+4(+(++((4+&0&))&05&&0&(+(+
@HWI-H225:69:CBJ27ACXX:8:1101:11390:2103 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAC
+
HFGEI9FGIGJJBGGI@CE9@?;>CB>ABBC=5::@A>@CDDBD(8@0:>>BDDD?<A>@ACDDDDDD@<0)&
@HWI-H225:69:CBJ27ACXX:8:1101:11328:2134 2:Y:0:AGTCAA
TGTAGCGTTGGTGTGTTCTGACTCAGATCCGAAGAGCGTCGGTAGGGAAAGAGTGGAGATCTCCGTGGTCGCC
+
86/)0?A'7-((-((..).7=777;7)..(.;;39=5;'(,0)2;?<3(2(((+++:89(++:()++&+())8
@HWI-H225:69:CBJ27ACXX:8:1101:11563:2110 2:Y:0:AGTCAA
TGTACCCTTGGGGCTCCCTGCCGCAGATCGGAAAAGCGGCGTGCAGGGGATGAGCCTAGAACTGGGCCGCCCC
+
0''.)8..=).('''.(,).((,''88((,'5(&(+<5537))&238<&)&(+(4(+49@(:>4>82+&)0&)
@HWI-H225:69:CBJ27ACXX:8:1101:11905:2002 2:Y:0:AGTCAA
CCTCCTGGCAACAACGTCGGGAGAGCGTATTTTTTGGGAAAAGTGTAGATCTCGGGGGTAGACGCTTCCTTAA
+
****0)**()0(/8('..--'5',;A8'((-(-5''&&(+((++((((+(((((2&)9&)2(4-&&+&(++(4
@HWI-H225:69:CBJ27ACXX:8:1101:11884:2020 2:Y:0:AGTCAA
TCCTTTTTTTCCTCTTTTTTTTTGTCTTTTTTCCTTTTTTCCCCTTGGGTTGCTAAGGCCTCCCCAACGGGAC
+
-)8)8@=;5'))((..;A.9=8&&++(3((+5(3(+:+45(:43(2(91((&((++(+(((((()(((()&&0
@HWI-H225:69:CBJ27ACXX:8:1101:11889:2043 2:Y:0:AGTCAA
ATCTCCATGGGGGTTGTTTTTGAGGTTTTAATTTTACTTTCCACTAAGGAGTAAAATGGGGTGCTAAAATGGG
+
2+22+<*19*1?00(('().(-(5=)(.()).).77))))).(.(.;-((,5(,;5(,(((,&(4(+4((+:(
@HWI-H225:69:CBJ27ACXX:8:1101:11972:2115 2:N:0:AGTCAA
GCCCCTGGGAACAAGGTCGGAAGAGCGTCCTGTTTGGAAAGAGTGGGGATCTCGGTGGGCGACGGAACATTAA
+
)***0(//((.76C)(.7;4'5'.(.9=1',(5(((((,(5(,(4+(0+0((+(2++5089&)&)&()(+>4>
@HWI-H225:69:CBJ27ACXX:8:1101:12155:2034 2:Y:0:AGTCAA
CTGGTTAAATGCCCCCATTTGAGGGCTTTTTTTTTTTTTTTCTTTTTTAAAAATGGTAAAGTGACATAAAGGG
+
))))).))77=4@?AE>>@?3.,6('(5>@A?B3;B>0&&&+(((+(08>4(48((((+44:(4(++44>3(+
@HWI-H225:69:CBJ27ACXX:8:1101:12162:2051 2:Y:0:AGTCAA
GGGGGTTAGTGTGTTCTTTTTCAGATTGTTTTTGTTTCGTTCATTGTTAAAGATGATAAATTCGTAGACAGCG
+
'-''&6)((3@((+(33((((+(33+((+(+((&+(+((+3(((((((+4:((((((+++++4(+(((+(+()
@HWI-H225:69:CBJ27ACXX:8:1101:12078:2055 2:Y:0:AGTCAA
GAGAGGTCTGACTCAAAGGAACAGAGCATCGAAGAAAGAAACAGTGTAAGATTAGTTGGTCCCCTGATCGCTA
+
)*)*1)11:B9B*****0)(/(0).=)..=-))-;7)7.())..7).;(((.(((---((((((((,,(((++
@HWI-H225:69:CBJ27ACXX:8:1101:12120:2145 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAACAA
+
G>FDHHGHJII8DAGGEEE946;>@B9=A@>C>:@CCDACDCDD<BB):9@B&))82(::+:C:35&95)&+8
@HWI-H225:69:CBJ27ACXX:8:1101:12130:2166 2:Y:0:AGTCAA
TTGGCTCAGAGCCGAAGAGCGTCGGGGAGGGGAACACGGTGGATCCCGGTTGTTTCCCTATCATTAAAAAAAA
+
)*9(*))8B)))(5''-5A)9',','''))&&0&++()08(0((((++&)&&)+(,:((+((+44:@:ACB90
@HWI-H225:69:CBJ27ACXX:8:1101:12426:2082 2:N:0:AGTCAA
CTTTTTTGAGGGTTTCTTTTTTGGCCCGGCGTCTGGGGGCCAGGGGCCACTGGTAACTCACTCGGGTTTGTCC
+
))))/B''(;)(,.(.;>(((','(((0&)&))08?9>&&&+8<@;&&(((+<+:((+((3(+(<&+(2(+((
@HWI-H225:69:CBJ27ACXX:8:1101:12441:2104 2:Y:0:AGTCAA
CTTTTTGGAGGGTGCTTTAGAGGGGGAGCCCTTTGGGGACAAGGGGCCCCTGGTAACTGACTCGGTTTTGGCC
+
3B@G;@-'.)('(,((.66(..,9'85&&2(3(+((0&258<?B8559&0(+<3:@A>((4(48(200?8&(+
@HWI-H225:69:CBJ27ACXX:8:1101:12890:2104 2:Y:0:AGTCAA
CAGATCGGAAGAGCGTAGAGTAGGGAAAGAGAGTAGGTCTAGGGGGTAGCCGTATCACTAAAAATAATTGTGG
+
91CD49:D)86(9)('-5(/))).(7..6?7)5?);(;3;6((,,;3*8(())01(((+4(++((4(+((+(+
@HWI-H225:69:CBJ27ACXX:8:1101:13142:2015 2:Y:0:AGTCAA
TGTAATTTGTCGCTGGGAACAAAAGCGGAAGAGCGGCGGGAAGGGGAAGAGGGTAGGTCGGGGGGGGCCCCGT
+
1)0*0*0*9*08B:@61()5.8(()('-''9(6(9''0&))&+(00&)+(((&((2(((&&)5&050&&)&&)
@HWI-H225:69:CBJ27ACXX:8:1101:13132:2030 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAAGTAAAACTCCGTGGGCGCCGGTTCCTTTAAAAAAAAAAAAT
+
IIIFG@F?A7FGGGGIBGE,96.6>B>=A(((((,(,(((((,++&&+&)5&0&&((4:(+>>:@&&)0&&&(
@HWI-H225:69:CBJ27ACXX:8:1101:13057:2174 2:Y:0:AGTCAA
TCCCTTGGGAAAGAGATTTGGAGAGTGTCCGTTATTTATATAGTAATTTTTTCGGGGGTCGCCTTTTCATTAA
+
4).))).7=A@((76(>((.(((-;55((((,,,(5(+(+(((4(((((((0(&)099))0&20&+((,++(+
@HWI-H225:69:CBJ27ACXX:8:1101:13065:2200 2:Y:0:AGTCAA
GTTTTGTTTTGTTTTGTTTTGTTTTTTTTTTTTTTTTTGTTTTTTTTTTCCTGTGGGGGAAAGTAAGGCTGCT
+
'..)8267C@((.=);@CE9(;@A6(3300;<&)57>@&2<CCBB39&&+(+((+(099-&2++44(:((+(4
@HWI-H225:69:CBJ27ACXX:8:1101:13455:2006 2:N:0:AGTCAA
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCCCTTCATTTAAAAAACAAACAGGGAA
+
DD;@?;)?2?D7CB'-(/=);).=?@;).7?@;>;6>AB(=>',8;B&&(+((4@((4>:49&+(2(2(((((
@HWI-H225:69:CBJ27ACXX:8:1101:13294:2007 2:N:0:AGTCAA
GCCTCTGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAAAGTGTAGATCTCGGGGGGCGCCGTATCCTTT
+
;@GG)=);;;C?3AE3>;7?A(5,5,5;'5/&0+(+:?(2<(+)(4((((((4:2?&00&&)&0))&++(+:(
@HWI-H225:69:CBJ27ACXX:8:1101:13271:2232 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAACAAA
+
?DGD?F?2;;@;(B@<E;49<CA6;=;5;;((>5>555>:@ABB8@@5(9<B959<8(:C4>AAC@B.&&(+(
@HWI-H225:69:CBJ27ACXX:8:1101:13736:2108 2:N:0:AGTCAA
GCCGCTGGGCACAAGATCGGGAAAGCGGCGAGTAGGGAAAGAGGGGAGGGCAAAGTGGTGCGCCCGCCCTTAA
+
5@C'@@BE?()(6(((6-(3','((('&*0&002:+2(82((((&&)&&&&(++++:A4<(&0)&&&)&&+4:
@HWI-H225:69:CBJ27ACXX:8:1101:13545:2154 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
GGIGIIHJIJJG2@FHAEE=B?>CEDCBD=AC:A@CCDEADDDD@DB<AB>BB59<A(>CACDDDB<<99><>
@HWI-H225:69:CBJ27ACXX:8:1101:13580:2192 2:Y:0:AGTCAA
ACTTGTTGGCCCAGAGATTGGAAGAGCGGCCGGGAGGGAAACGGGGGAGGTCTCGGGGGGCCCCCGATCAATA
+
()//8/=(=)7=1=(..).)6..(6(('','&&&&)08&(2(+(&5&&&&++(+(2&&)&&&)0&)&)&&(4(
@HWI-H225:69:CBJ27ACXX:8:1101:13582:2243 2:Y:0:AGTCAA
TCTTTCACTTGTTGGCCCAAAGATCGGAAGAGCGTCGTGTAGGGAAATAGGGGATATCTCGGTGGTTGCCGTA
+
').@E)773.77A2;77;;((555>BB(('(,(3)8555?(+:?CA?(+442&)&(+(+(+2((050<1>9)0
@HWI-H225:69:CBJ27ACXX:8:1101:13959:2057 2:Y:0:AGTCAA
CTTTCCGCCCTAAGGGAGGCGAGGACCCTCTCGTGGAGCCAGATGGGAAATTTGGACGCCCGGCGAGTGGGGA
+
)(/0))('---.7).6.96('''''((((+(+)(()(++(((+(+(2(+((((+((40&&&00&)&&&(((&(
@HWI-H225:69:CBJ27ACXX:8:1101:14094:2057 2:N:0:AGTCAA
CCAGAAAGCTTCCATTCCAACAAGCTGCAGAAGACAGTATCTGAACAACACCATTGACAGTATTTGCGTTGGT
+
AHF9FHBEGHI@E>EH;A@DB;?AAC;AC;A>>C?:953>@DDD@:@C2228?AC4::(4:>CCD>><<@3<2
@HWI-H225:69:CBJ27ACXX:8:1101:14209:2182 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGTATCCTTAAAAAAACTTTTT
+
@D:D9**0-7BF.(.;'54=966;AC35?AC((',>?:;(,(828?9):&0<))5(2((:+>>3())&(((+(
@HWI-H225:69:CBJ27ACXX:8:1101:14468:2063 2:N:0:AGTCAA
GTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGCCGGATC
+
FGHBG(;(;CF@@4)=D=3=DEE2:??<6>A8>?@/899@A@(288?3++((299:@@?B?B<&:&085&)0.
@HWI-H225:69:CBJ27ACXX:8:1101:14272:2185 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
IIJJGIGIGBHIJJFGEEAFGBA;FHBEEDEFF;@CCCC(;5>;@CCDDDD?BB0:9<?<&0<A(:A>>ACDB
@HWI-H225:69:CBJ27ACXX:8:1101:14359:2243 2:N:0:AGTCAA
GCTTTTTAATTGTTTAACTTTATTACTGTTGCACCATTTATACAATTACATATAATTTCAATGCATCCATTGT
+
BAGGHIGCECEC7AECECDFF3;@BEE>@C;;;>CDDCDC>;:;ACC>::;>>>ADDE::C>3>C@:?CCD3>
@HWI-H225:69:CBJ27ACXX:8:1101:14392:2243 2:N:0:AGTCAA
TCTTTGACTTGTTGGCCCAGAGATCGGAAGAACGTCGTGTAGGGAAAGAGTGTAGATTTCGGTGGTCGCCGGA
+
'7@=@@.?;EHH<6?DF@7(.5;;;A:@8'5,92<885589:@@A?A?(3+4((+4((4(+9(+0&+0&80&)
@HWI-H225:69:CBJ27ACXX:8:1101:14518:2068 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAACC
+
:FFGG*0BGHEB@CGG@@EBB;>>AC3;;=;?(:(,:@CCDD??(2<&:55@9)5(<:@@::C@C05&0&)&(
@HWI-H225:69:CBJ27ACXX:8:1101:15116:2033 2:N:0:AGTCAA
CATTTGTCTTGTTGACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG
+
FADHGEF@EG4=@?3.?C>?B;2;>A?=AAC>>=?BDB<?<;9B>9@A<:@B98<839(4:3((:(4322+85
@HWI-H225:69:CBJ27ACXX:8:1101:15123:2159 2:N:0:AGTCAA
GTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAA
+
BFEHHJIJJIJI=FGHIJJJEIGEEHHFCBA=?CDDDDB??C(:(5:@A>CDDD2<>5:55@9&5A1>C:>CC
@HWI-H225:69:CBJ27ACXX:8:1101:15234:2237 2:N:0:AGTCAA
CTTCCGATCTGACTCGAGTCGACATCGGAAGAGCGTCGTGTAGCGTTGGGGGGTTCTGACTCAGATCGGAAGA
+
HCHCBAFEDHHEGCHBGHIIG@:A<EE>DCBEA=9>B=B@?CCA>@?>8&5&09@2@@A>>ACCDDCDB<85A
@HWI-H225:69:CBJ27ACXX:8:1101:15490:2096 2:Y:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
IIIFJICEICG>9BFHCGH2.=5'5@4:A;?>B',',52-(-5>3;:,:9<,1&0499B05>(+:4((4+::9
@HWI-H225:69:CBJ27ACXX:8:1101:15472:2141 2:N:0:AGTCAA
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAGG
+
GIGIDH)?)?FFD@F<CA@@G)6??@;7?@;@AC:>@2=?;>;>==?-0&88@:@+:>A>@<90&&))&&&)(
@HWI-H225:69:CBJ27ACXX:8:1101:15921:2242 2:N:0:AGTCAA
GGGATTTAGTTTTTTTTTATTGAAACGGTAGGGGGAGTGTTTAAGACAAAGGGAGATCGGAAAAGCGGCGTGT
+
/;<==@C7).=).5'38;555(3(((8<+()+(07&82+(+(((+(+(9?93++&&++8>(()332&&&)0.5
@HWI-H225:69:CBJ27ACXX:8:1101:16221:2095 2:Y:0:AGTCAA
TCCGCTGGGAACCAGATCGGTATAGCGTCGTGTTGGGAGAGGGTGTAGATTTCTGTGGACGCCGGATTATTAA
+
5@)('':/;;;5(6==;3=B5((,5:=8()0)<(+(&2+&8(5(2)(+::(43(4(+((+&))&&&0&(+4(:
@HWI-H225:69:CBJ27ACXX:8:1101:16324:2086 2:N:0:AGTCAA
GACATACAGGTTAGGTTTATTAATGGACACACTGGAAAAGAGGATAGATCGGAAGGGCGGCGGGTGGGGAAAA
+
/5@7.=)))).=)(..;;)77(6.;6;>>=3,;CB:A((5(5<1(,((((+)+8<()(5&&&&&&&&09()8?
@HWI-H225:69:CBJ27ACXX:8:1101:16436:2188 2:Y:0:AGTCAA
CTTCCTATCGGACCCGGATCGGGAGGGGCGTGTTCTGAGTCAAATGGGAAGAGCGTCGTGGAGGGCAAGAGAG
+
*00990098(7;''-;''',','3'''3'0)))2(:>4:(++(((((2338?1<))(009-8(25&(((38(+
@HWI-H225:69:CBJ27ACXX:8:1101:16497:2224 2:Y:0:AGTCAA
CTGACACCCCGTTTGTCCGTATAGCTTTTGCTGTCCTGCAAATCACTCCTTTCCCCAGGCTGGCCTCAAACTC
+
)))/).=--''5(7(6;(((,(--;((;5,(,(,(5(:CC@(+((+(4<3:(,(+(+2<8+85?8((+:4(>(
@HWI-H225:69:CBJ27ACXX:8:1101:16570:2136 2:N:0:AGTCAA
TGTAATGTTTGCCTCTGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC
+
GGDDEIGGIIIBF=DFHGG@CADGHEEEHHF?DC@ACB3,;2=9?ACD38?1394>(+4((+:@BD28>0455
@HWI-H225:69:CBJ27ACXX:8:1101:16966:2068 2:Y:0:AGTCAA
TCAAAACAGAAAAGAGAGGGTGGGGGGAAAAGTGCCGGAGTCTGGGTTGCCCTGATTATTTTAAAAAAAAAAA
+
****118:)0?*9*)))/?(((-5''&)50(+(3((&)&)29:A(9++&(+(((+(+4>B>:(:@(>&&09@&
@HWI-H225:69:CBJ27ACXX:8:1101:16965:2148 2:Y:0:AGTCAA
ATTGTGCCATTGAACTCCAGCCTGGGCAACAGAATGAAATTCTGTCTCAAAAAAAAAAAAAAAAAACCCTTAA
+
GGE@9D?<DBDD?BDDGHCH@??FGGIJIIGGGIJGGAECHHHBCDFFEFDEDBDDDDDDDDDDDD&288?C3
@HWI-H225:69:CBJ27ACXX:8:1101:16985:2183 2:Y:0:AGTCAA
TCTTTCTGTCCCACACAACGGGAGATCGTTTTTTTTGGGAAATGTTTGTGTTTCCGGGGGCAGACCGTCCTTT
+
00/B****/).8)8--(((''',',(.6.((5(,&)&&&+(+(+4(+(+(+((+((&&00)&)(((&0)&(+(
@HWI-H225:69:CBJ27ACXX:8:1101:17028:2032 2:Y:0:AGTCAA
GTGTGGTTTGGCTGCCATACTACTCTATTATTCTAGGTGTGGGGTGTATTTCTAACTCACTTGATGTTTGTCT
+
*?;BD79))7(?*7;;3B))..))).7))).))=).3)..(53'9/,,;5(;;3;(++(((++(+++4@+(((
@HWI-H225:69:CBJ27ACXX:8:1101:17069:2067 2:Y:0:AGTCAA
GCGGTGTGTTTTGACTCAGATCAGAAGGGAGTCGGGTAGGGAAAGAGTGAAGATCTAGGAAGGAGCCGTTTAA
+
((->'',((.(,(,-5(5:(,5((((,(0+&((&&0+)2((+88<9((+((((+4((+4((+(++((&&&+((
@HWI-H225:69:CBJ27ACXX:8:1101:17022:2103 2:N:0:AGTCAA
GCTTTGACTTGTTGGCCCAGCGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTGGCCGTA
+
'=F@FF@EDG>EH;=7?B?B(,,5,;@<?'5255&25&5((4@B382839(&(((+:3+41<889&(((2&&)
@HWI-H225:69:CBJ27ACXX:8:1101:17215:2174 2:N:0:AGTCAA
TCTGCTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTGGGGGAAAAGGGTAGAACTCGGTGGGGGCCGCGTC
+
6).)7.))).)=)..).??;366(3(,'59(8,,077?((050&)(+((2((++(+4((((+50&505&&&*0
@HWI-H225:69:CBJ27ACXX:8:1101:17399:2164 2:Y:0:AGTCAA
ACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCCGGATCATTA
+
BCFGCGI@ECGEEFEHHFFBD1;8A=?B@BB==??CCB(8A<99(:+:>@C>AAAD&5>)0<>?<&09><CC>
@HWI-H225:69:CBJ27ACXX:8:1101:17562:2016 2:Y:0:AGTCAA
GTGTGTTCTGACTCAGATCGGCAGAGCGTCGTGTAGGGAAAGAGGGAAGATCTCGGGGGGCGCCGCATCATTA
+
H9B)??BF?D?CCE9@CGIIJ:@ACDDB>C?,89C::<(,993929&2284::A<@-9B&59<B9&05<:(4>
@HWI-H225:69:CBJ27ACXX:8:1101:17529:2107 2:N:0:AGTCAA
TCCAGAAAGCTTCCATTCCACCAAGCTGCAGAAGACAGTATCTGAACAACACCATTGACAGTATTTGCGTTGG
+
IGIG@EHHCEHEDEDDFF@B(;;A=?CAACACD:>?CA:@A>CACC@@<88<<?@CAC:CC@ABCD>AB>@9?
@HWI-H225:69:CBJ27ACXX:8:1101:17772:2063 2:N:0:AGTCAA
CTTCCGATCTGACTCGAGTCGGAAGAGCGACGTGTAGCGTTGGGGTGTTCTGACTCAGATCGGAAGAGCGGCG
+
DBF<D08DFH<DFG@BFH@FBBAA/=>)5',99?>(:3/38&2&&)&)2(4((+:>(,+(+2088521(&00.
@HWI-H225:69:CBJ27ACXX:8:1101:17859:2186 2:Y:0:AGTCAA
TGTCCAAAGATCCGAAAAGCGCCGTGGAGGGAAATAGTGGAGATCTAGGGGGTCGCCCGGTCATTAAAAAAAA
+
)*1:C*000*:<*0)/('.8;'54=A'5((,'(,(((,5(((,,((,5(()5)&)&+&&&)&&+4(((4>>B5
@HWI-H225:69:CBJ27ACXX:8:1101:17979:2189 2:Y:0:AGTCAA
GTAGATGGGAAGAAGGTCGGGAAGGGAAAGAGGGGGGAACTCGGGGGGGGCCGGATCATTAAAAAAAAAATAA
+
)0?;4C)8=@D=A))(.=B/'',5;?2;??59('&&)&&(4>@B--&&)))00&))0(4>(44>@B5&)&(,4
@HWI-H225:69:CBJ27ACXX:8:1101:18196:2238 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCCGTATCATTAAAAAA
+
GEG*?CAB>B:;B@F>?FG@8)5;;B45=@?DB;2;@>?-5;55;5,,,8,,5;&(0020002<>@D@:A:AB
@HWI-H225:69:CBJ27ACXX:8:1101:18297:2247 2:Y:0:AGTCAA
TCTGCTTCCTGTCAACACAGATCGGAAGAGAGTCGTGTGGGGAAAGCGTGTAGATCTCGGTGGTCGCCGTATC
+
'@7;7=;>37==)).7;(;>;..5;=5/,,(,552?3(++0;(0(8(525-9((:((((+2(5)4)&8.&)2(
@HWI-H225:69:CBJ27ACXX:8:1101:18548:2134 2:Y:0:AGTCAA
TATATTCAATACCCCCTTTTGGGGGCTTTTTTTTTTGTTTTCATTATTTAACATTCTAAAGTCACATACAGTG
+
F)**00*8*/)).=;4'==D>'''''3;CC@;7-0;)<3(9(((4(:4((+((:4(4:AA(:(+4(+(+(4(4
@HWI-H225:69:CBJ27ACXX:8:1101:18601:2206 2:Y:0:AGTCAA
TCGCCCGGAGATAGGAAGCGCGGTGTGTTGGGAAAGGGTGGAGATCACGGCGCGGGCCGCCCTCTTCTAATAT
+
')'5>'',3/>((((:@((,'&&&)&28(+(52328(+((&(++(+(+0&&&&&&)&)&&&&&(++((+((+(
@HWI-H225:69:CBJ27ACXX:8:1101:18877:2224 2:Y:0:AGTCAA
ATAGATCGGAAGCGCGGCGCATAGGGAAAGACGGAAGATCTCGGTGGGGGCCGAAACATTTAAAATATGAGGT
+
*:?*::4?66?(?('5'''''',5@9>A2(9(&)&+5<(((((+++&&))&))&&&&+((((++:(((4(++(
@HWI-H225:69:CBJ27ACXX:8:1101:19250:2069 2:Y:0:AGTCAA
CGTAGCCTTGGCGCGTTCTGCCCCAGACCGGAAGAGCGGCGGGGGGGGGAAGGGGGGAGAGCGCGGGGGGCGC
+
''---;))))733'',3(((:5(22(((+&&&)028()&)0&&&&))0&)&2(00<<&)&(+&&09&.<&&)&
@HWI-H225:69:CBJ27ACXX:8:1101:19242:2105 2:Y:0:AGTCAA
TTTCGCCCAGAACTAGGAAGCAAGACGGGTATGGAAAGAATGTAGATCTCGGCCGGGGCCCGCTCATTAAAAA
+
A',)43,,'5=3;((,,5(,,((+(3)&&)&(+(((((+(((++++4(4(+0(&0&&&&)&&&55&(+((+(>
@HWI-H225:69:CBJ27ACXX:8:1101:19066:2245 2:Y:0:AGTCAA
CAGATCGGAAGAACTTCGTGTAGGGAAAGAGTGTATATCTCGGCGGACGCCGTATCATTAAAAAAAAAAAAAG
+
FBB<?986BGD9*8)/8B);(=7)5)6=A7=.?)7)))766;=(,5-&)&&5))+((+:3>:C35@9&&&0&&
@HWI-H225:69:CBJ27ACXX:8:1101:19423:2208 2:Y:0:AGTCAA
TGTCCACACAGGGGGAAATTGACGGCGTTAGCACTGAACACCAACAACGCACAAGTGTAGAACCGCGAATAAG
+
?B**//.)((..;''',(.6.((''',,76(((3>(((4(((++(+((&&*)&+(+(44>3:+(&&)&)&++(
@HWI-H225:69:CBJ27ACXX:8:1101:19562:2212 2:Y:0:AGTCAA
CCAGACAGCTTACATTCCACCAAGCTGCAGGAGACGGGATAAAAACCAAACCATTGACAGTATTTGCGTTGGT
+
3=))/((.(...7?37)).)....,(.-((((,,((3'0)+((((&+8(2(2(++:(((,((4:4(+&5<&+(
@HWI-H225:69:CBJ27ACXX:8:1101:20050:2027 2:Y:0:AGTCAA
TGTTCCCGAAAATAGGAAGAGCGGCGTGGAGGGAAAGAGTGGAGATCTCGGGGGTCGCCGAATCATTAAAAAA
+
D9***11))8).***0)/((.)-'5=3''(5??2?9((++4((+(+((+2?9@>&005?5&)(+44(:@CDC>
@HWI-H225:69:CBJ27ACXX:8:1101:20281:2015 2:N:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
GIIJIGJHIIIJIJJGBED@@?CCDDDDDBDC>ACACDDDDDDD@BDBC@BB<<@BBDDD>>CCA-9>5)&)0
@HWI-H225:69:CBJ27ACXX:8:1101:20256:2070 2:N:0:AGTCAA
GTGTGTTCTGACTCAGATCGGCAGAGCGTCGTGTAGGGCAAGAGGGTAGATCTCGGTGGTCGCCGTATTATTA
+
;*?)?8D9?D>B).83BDCGA8;-56.5',9;?2(5>@(,5892()(+(+(+4+2<(8@0:95<>-.8(+:>B
@HWI-H225:69:CBJ27ACXX:8:1101:20634:2018 2:Y:0:AGTCAA
GACGCTGGGAAGAAGAAAGGAAGAGCGTCGTGTAGGGAAAGAGGGGAGAGTCCGGTGGGTGGCGGTTAAGTAA
+
-()---''(.7((((.((-55(59((,,(&&&+(+3<((++(3255>>-(((+()&)0&&0&2&&))&+(+(+
@HWI-H225:69:CBJ27ACXX:8:1101:20525:2101 2:Y:0:AGTCAA
TCGGAAGAGCGTGGTGTAGGGCAAGAGTGGAGAGGTCGGGGGGGGCCCCAGCGTGAGAAAAAATACATGCTAA
+
**1)16))93)0(78B(8)8C35@()(.=()(;((.;''',9&)050&)2((&+&((++(+(&(++((((++(
@HWI-H225:69:CBJ27ACXX:8:1101:20996:2002 2:Y:0:AGTCAA
TGTTTTTTGGGTTTTTTTTTGATTTTTCCGCCAAGGGTTTTCTTGGACGCTCCCGGTTTCCTTGGCATCACTT
+
'5@EEHDDA>A=@?7?BDB9&(((3>(9((&+)&(28((+2((((((()&&&&()&)&&(+((+((+((((+4
@HWI-H225:69:CBJ27ACXX:8:1101:21037:2066 2:N:0:AGTCAA
CTTGTTTGCCCAGAGATCGGCCGAGCGTCGTGTAGGGAGAGAGTGTCGATCTCGGTGGTCGCCGGCTCATTTA
+
:CG>B?)?9B?B??()99B'7@/;8/43=;''555(<:',822:>:@@8<1<C??8@<53&)+5&)5.(4@:@
@HWI-H225:69:CBJ27ACXX:8:1101:21033:2121 2:Y:0:AGTCAA
GGTATTTCTGCTTCCTGTGGCCACAGATCGGAAGACCGTCGGGTAGGGGAAGCCTGTAGGTCTCGGTTGGCGC
+
''='3C@E;)7?).)).7?)6;;=(53;?92(582(,55&0&0<&2+2&&)(((+++:>(4((4((+&)&())
@HWI-H225:69:CBJ27ACXX:8:1101:21012:2126 2:Y:0:AGTCAA
TGAGTTGCCAAGCGTCGCGTACAGGGTACATTTGTGGGTTGCCGCCGCTTACCCAGTAGTTACAGCTGGACGC
+
(--())..))).(;((',,'',(5,8(((((+::3((0&(&((&&&&&)))((+4((((43((((+433(()0
@HWI-H225:69:CBJ27ACXX:8:1101:21291:2053 2:Y:0:AGTCAA
CGCTCTAGGAGAACGGAAGTGTGGNNCGCAGGGATGGAGCGTAGAGACTGGCGGTCTTTCCAAAACTTAAATA
+
''--4A.7))).(.',(53((-,,##,(,((&)(((((((&&()((((+:?(&&&&&+((+((((((+(+(((
@HWI-H225:69:CBJ27ACXX:8:1101:21261:2209 2:Y:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGNAAAGAGGGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAATAATTA
+
<GBG>FGHICE@(--559A>?((;>#,,551<(5?(8(3(4:28(250(05<-&&(+4:+(+(:@&&((+((+
@HWI-H225:69:CBJ27ACXX:8:1101:1133:2389 2:N:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGATCGCCGTATCATTAAAAAAAAAAAAA
+
@6@GDDEHIGGA(-'-5=A>@A;;>?>AB<CC5@AC:>(+>CBD??((++8<9&0??DCC@ACDDB>559&&&
@HWI-H225:69:CBJ27ACXX:8:1101:1439:2293 2:Y:0:AGTCAA
GTAAAACGGAATCGTGCCGTGACCTGTTATACCGAAAAACCCCCTTTTCTCCCCTCTATTTATAACTTCATTT
+
)08*?*?(((7-.((((6'-'(,))).)(((((389'89?(80&)(((+(+(+&3)(4+:4>3(((+4(>@>>
@HWI-H225:69:CBJ27ACXX:8:1101:1688:2447 2:N:0:AGTCAA
CCTCTGCTCCAGAAGATCGGACGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAA
+
3BCBFEGGFGGGHI>E;AEF;@<;>:@BB<57<AC:<>28+39+:>:(:>34>2?8?B5>@BB5&08<4>((:
@HWI-H225:69:CBJ27ACXX:8:1101:1787:2456 2:Y:0:AGTCAA
ATAGATCGGAAGCGCGTCGGGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
0?DDH*??FH=A=F6@==A'3,38<?(2,(:9((+(444(4++2((&&(008&0)(2:(:@CDACBBDD>B5&
@HWI-H225:69:CBJ27ACXX:8:1101:1780:2473 2:Y:0:AGTCAA
ACAGATCGGAAGCGCATCGGGAAGGGAAAGAGTGTAGATCTCGGAGGGCGCCGGATCATTAAAAAAACACACC
+
(('..).(55;4(5=''',9>''5</5((5((++33>(4(+(((&&)&&)55&&&)5+(4>34(40&&+((&(
@HWI-H225:69:CBJ27ACXX:8:1101:2037:2258 2:N:0:AGTCAA
TCCTTCCTGTAACAACAGATAAGAAGAGCGTCGTGGAGGGAAAGAGAGTAGATCTTGGTGGGCGCCGGATCAT
+
/)..=@C>..)...)(..;?))..63;63?9(,'35(2<7-82++3((((+(+(4(+:+28025<B5)055(+
@HWI-H225:69:CBJ27ACXX:8:1101:2218:2473 2:N:0:AGTCAA
AATAGAGGACTGCTCATTTTCCTTCCAGGTAATAGAAATAAATGTTCTATGTCTGTTAAACAAAAGACATCAT
+
BF<@C<?GF?DD;DG4?BGIHCCHGHGG@@FECD>DEHG:AECCHH)=;B:??C>AECCC>CDBA3;5::>CC
@HWI-H225:69:CBJ27ACXX:8:1101:2027:2476 2:Y:0:AGTCAA
CAACAGGTCGTTCTGCTCCTTCCTTTGGACACAGATCGGAAAGGGGCCGGGTAGGGAAAGAGTGGGTATTCCG
+
?*94)(())'-'(()))).7);?@9);3>((((,-5>?8(8'(5()&)5&-&&+88(12(+3+(2(&+((+((
@HWI-H225:69:CBJ27ACXX:8:1101:2378:2299 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAACAACG
+
BE>D>DDFBGIJ@D@@;;A@'.7>CDCAADCD@C>>A>ACDDB<<B&)+)5?9)5AACCA:ADDCB5<&2+(&
@HWI-H225:69:CBJ27ACXX:8:1101:2408:2397 2:N:0:AGTCAA
TTTGAAGTGCGGCGGGGAGGGGAAGAGTGTAGTTTTGGGTGGGAGCGGTGCTTTTAGAGAAAAAGAGAACATT
+
*)00)0*0*07(7-',)&)&5&05808(:(+3((+(()&())&+8&)))&&((+4((+((++((&+((+(+>:
@HWI-H225:69:CBJ27ACXX:8:1101:2902:2346 2:Y:0:AGTCAA
GCCCTTGGAAACAAGTCGGGAAGACCGCCGGTTTGGAAAGAGGGGAAATCTTTGTGGGTTCCGGTTTCTTTAA
+
F3))..7@)7=))(..)(,'3',(((,,&)&&)&+8++:(((()0&&(+(+4(4(+25&((+&0))&+(:>(>
@HWI-H225:69:CBJ27ACXX:8:1101:2950:2414 2:Y:0:AGTCAA
GCCTCTGCTCCACAAAAGCGAGAGAGCGTCGTGTGTGGCAAGAGTGGAGTTCTCGGTGGTCGCCGCCTCTTTA
+
@@=@)7))).7=)7;(().'(',358(',,&&&((++(((:(+2(((+((3+(+8<(25&(&&+5&)05(+++
@HWI-H225:69:CBJ27ACXX:8:1101:3085:2492 2:Y:0:AGTCAA
TTCCCTGATCCCGGAGATCGACAGAGCGTCGTGTAGGGCAAGATTGTCAATTTCGGTGGTTGCCTTATAATTA
+
1**111*):**))))(0.)8(''-.@3'5(5,'.,(-5(;<((((((,(5(((+90((&)((((((44(+4:(
@HWI-H225:69:CBJ27ACXX:8:1101:3365:2463 2:N:0:AGTCAA
CCCTCTGCTCCAGAAGATCGGAAGAGCGTCGGGTAGGGAAAGAGGGGAGATTTCGGGGGCCGCCGTATCATTA
+
*?*B?FB).)=@H@@).==DH(-'6(;3,5;',,31<@>88(++(0-9&)((4(3<09>&)&&)))028>34:
@HWI-H225:69:CBJ27ACXX:8:1101:3890:2258 2:Y:0:AGTCAA
ATAGATCGGAAGAGCTTTGTGTAGGGAAAGAGTGTAGATTTCGGTGGTCGTCGTCTCATTAAAAAAAAATATG
+
)?D;40B2((7'(.))=.7C@;)7)(.?(()..(6(6;A;;3=/?(',(,'+&0(((((44>CCCDD.0(+((
@HWI-H225:69:CBJ27ACXX:8:1101:3809:2310 2:Y:0:AGTCAA
ATAGATCGGAAGCGCGGCGCGAAGGGAAAGAGTGTAGATCTCGGCGGTCGCCGTATCATTAAAAAAAAGATAC
+
:)839B*?D;6-('-;'=>'8&))7@28A29?+33:(+4+4482(&505)0?<0)2(+:4+:ACC-0&&24+(
@HWI-H225:69:CBJ27ACXX:8:1101:4105:2370 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAATC
+
0)?9BDH@GGIIH@8;;DE5'.;;@A>=A2;3-5((,5((5:9@B?&):&&205&8<:>C(:>A>&)&&))((
@HWI-H225:69:CBJ27ACXX:8:1101:4376:2251 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGGGTAGGGAAAGAGAGTAGATCTCGGTGGGCGCCGTATCATTAAAAAAAAGATCG
+
:)?9B4BD?BGG@HIE5;E69.'5=15??A?C((,:A@>3>3+88?B&(099B55A<::::>C>C>90&((((
@HWI-H225:69:CBJ27ACXX:8:1101:4346:2358 2:N:0:AGTCAA
CAGAAAGCTTACATTCCAACAAGCTGCAGAAGACAGTATCTGAACAACACCATTGACAGTATTTGCGTTGGTG
+
@<**?FDEGB)..=@;;7C;1(7;?CHE);?37?9(;@CDD(5;(;,,(<?9>A(55@(:>::>3(&08)222
@HWI-H225:69:CBJ27ACXX:8:1101:4656:2316 2:Y:0:AGTCAA
GGGGGGAAAAAGCGGTTTTGTTTTTGTTACGAAGTAAATGATTCGTATGCTGTACATAGCTGTTATAAAAGTG
+
FHI)0)0088/0?@B59588<?(38&2?@3<8?02:@CBC:>C>0?(9<3>:>>A:>C@CD:CC:4>(4@(44
@HWI-H225:69:CBJ27ACXX:8:1101:4712:2329 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAAAACAGG
+
BHEGEFGIGH;@@FFI;CB?B;7;AC@?BA>A5;>CC;;:ACBD58>)+55<<&59>?AC4>@CC><9.&(((
@HWI-H225:69:CBJ27ACXX:8:1101:4713:2409 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAACATA
+
<F<DHG@27<6;CF)5BD6B9?;;>(6=;;>;35:>33;>@:??2<B&45-<@5-<2+>A:ACDCB@.&)+++
@HWI-H225:69:CBJ27ACXX:8:1101:4821:2267 2:Y:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGGGCGTCGTGTGGGGAAAGAGGGGAGATCTCGGTGGTCGGCGGATCCTTCA
+
)0?0.0-<(.)-=377BEE8(;'(3;;,;=,82(85;0;(2(320&&&&(4((+228<0(5&)&&)00++4(+
@HWI-H225:69:CBJ27ACXX:8:1101:4873:2384 2:Y:0:AGTCAA
TGCTCCATAGGATAGGAAGAGCTTCGTGTAGGGGAAGGGTGTAGATCTCGGTGGTCGCCGTATCCGTAAAAAA
+
?CF?*?****:**0?9*9((0..8=5.-.)7@7'5=B(5(5,((-5(-(5(,588(5&(00&+2((+2&2>>9
@HWI-H225:69:CBJ27ACXX:8:1101:4867:2408 2:Y:0:AGTCAA
GCCTCGTTGCTTCTGTGTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTT
+
'3,'''(((',((,5,:38((+++(8<@&55;;5<<B-05850&)00>555-99--&0&&&))+39<00&&&)
@HWI-H225:69:CBJ27ACXX:8:1101:4979:2496 2:N:0:AGTCAA
GCTGTTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTGGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC
+
')/)).B.7)7=3=C)7.;.3;>?A??6(,5;'(&)0((+5-+982(((4((+(4(4(8?0(5&40.<&&)<(
@HWI-H225:69:CBJ27ACXX:8:1101:5447:2298 2:Y:0:AGTCAA
GGACTTAACCTGGCTTTCGGGTCATCCCGCCCCGCCAGTTCTGCTTCCCAGATCGGCAGAGCGCCGTGTCGGG
+
/'',,;-5@(((,((,,(,5&(0(+++((0&&)0&5&54(:(+(44>C?3((+++&&&&2<C&&59<@28&&9
@HWI-H225:69:CBJ27ACXX:8:1101:5450:2318 2:Y:0:AGTCAA
GACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT
+
D>FBD9?@@AFA26BD;FEEGIDCBC?CD==9,;;?CA?3<?A9(((4344:(44<?(2<&4<3<<&&<1@:A
@HWI-H225:69:CBJ27ACXX:8:1101:5272:2371 2:Y:0:AGTCAA
ATAGATCCGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTTGTCGCCGGATCATTAAAAATTGACAAT
+
1):)99?*(?FH08=4'-==A(7?3@(;A6;?-;(55(;5>:A8<'&)(0(2&&&5&2>444((4(((,(+(+
@HWI-H225:69:CBJ27ACXX:8:1101:5386:2434 2:Y:0:AGTCAA
ACAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
H(7;=CHEIGI@2':?9;@8?<A@CD>ABAAC3>3>3>@@>@<B<@D9:@@D5)5A?ACD@CCDD999&)&&&
@HWI-H225:69:CBJ27ACXX:8:1101:5436:2486 2:Y:0:AGTCAA
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAACTTGTTG
+
1??D7?F>2BG@@85A6=A3?)6;(7;;6;36(6;;AB@58',:89<&9-+<CDC>@::>09>B50&(+((+(
@HWI-H225:69:CBJ27ACXX:8:1101:5710:2368 2:Y:0:AGTCAA
ATACGTCGGAACCGCGGGGCGTAGGGAAAGGGTGTAGTTGCCCGGGGGGGGGCTGTAAGGAAAAAAACAGCGC
+
.)))(/8/''('(3='''))&5&)(8(((+22880(+((((,()&5--&&&&&)&+((((+(33250&2(()0
@HWI-H225:69:CBJ27ACXX:8:1101:5725:2376 2:Y:0:AGTCAA
GGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAA
+
B9*9B@GEDCHHCHIAGE;C6=?9ABD?CCDCBB?(55:(;>C::A:AB8<>9><5299-+24+:>>C@C9&&
@HWI-H225:69:CBJ27ACXX:8:1101:5688:2443 2:Y:0:AGTCAA
GCTTTCGAGATGACTTGTTTGCCCACAGATCGGAAGAGCGTCGTGAAGGGAAAGAGAGAAGATTTCGGTGGGG
+
*9*9*9(/)(-.)8)87==)7=)))(-.7))6=55',(5;38'')(((+&(+2(+9(2((((((4(()(+500
@HWI-H225:69:CBJ27ACXX:8:1101:5876:2301 2:Y:0:AGTCAA
AGGAGTCTTTTCATTTAATGCTTTTGAAGCTGTGGGGCGACCCACTTTCCCTTTGCCTTTCACTGGACTTGTC
+
0)))0BB?*?94))/8)/.=7)./7);=))7AEC>6=,/'&)-<22>>>:@>@@+(+:@A344(+89?CC3+:
@HWI-H225:69:CBJ27ACXX:8:1101:6097:2476 2:Y:0:AGTCAA
GCAAGAGTGTAGCTCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAATATAAAAGAGATTTGATGCGGGGG
+
)006?BF@88)8==CHG';C;'57=B?'39AA(>@33>CC6@>@50&&&((++44((+(+(+:(+(((&&0&5
@HWI-H225:69:CBJ27ACXX:8:1101:6485:2287 2:N:0:AGTCAA
TCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGGCGCCGTATCATTAAAAAAAAA
+
H9C@DGHGDDFBGGH6CCDH-5--:=>?BD;@?;6.(,,(5:3((55<,85&(&&0)&)884(:+(>@><30&
@HWI-H225:69:CBJ27ACXX:8:1101:6921:2376 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
FH8BDGIIDGGGHHAHIGH89?;@DDEEDBDC;@CDDDDDDCBDBDD5AB<B<<9B?ACD:CDDDDD@.5)&&
@HWI-H225:69:CBJ27ACXX:8:1101:7196:2418 2:Y:0:AGTCAA
GTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGCGTTGATCTCGGTGGGCGGCGTATC
+
(?/;B(-=(...)7)77)..))..',;',(5,,,,'0<((:2(82<>(()5(5(+(+(+(2(&&+&&)&)&((
@HWI-H225:69:CBJ27ACXX:8:1101:7277:2311 2:Y:0:AGTCAA
TACACTAGGAATCTGATCGGAAGAGCGTCTGGGAGGGAAAGCGGGTCGATCTCGTTGGGCCCCCAATCATTTA
+
-())))).))8)))))).-'(,,(.6335',9(((',58(++&&&0&)&2(8>((+++&(()&&&+9(+(:(4
@HWI-H225:69:CBJ27ACXX:8:1101:7474:2333 2:N:0:AGTCAA
GTGGCGATTAGTGTAATTAGTCGGGCTCAGGCGTTGGTGTGTTCTGCCTCAGATCGGAAGAGGGGCGTGTAGG
+
/=FEAG=A=?@)).;>CC>;(('3;;=?CCD<;5;<A(+++2>(::(4+(43>A?<<??-+2()&)5&503:@
@HWI-H225:69:CBJ27ACXX:8:1101:7426:2398 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGAAAGAGTGTAGATCTCGGTGGGCGCCGTATCATTAAAAAAA
+
E1D:CDGGIE@HBFFGCHFFE>BFEEEE?DFFBEEAEC;A>5>A@@CC?B<?B&8-599&)8?C>C4>ACCBD
@HWI-H225:69:CBJ27ACXX:8:1101:7288:2416 2:Y:0:AGTCAA
TCCGCTGGGAACCAGATCGGAAGAGCGGCGTGTAGGGAAAGGGGGTAGATTTTGGTGGGCGCCGGATCATTAA
+
/;C-;@=EB?>??A<2>?23;(3(,5'&)0))&(3:<(+28(2&0)0243+4((0+82&+&&0))&))(4(4:
@HWI-H225:69:CBJ27ACXX:8:1101:7423:2448 2:Y:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAA
+
/)8;//5A=(7,.>6;>;ABDB29(52'(0&020<@D3<A<(((+(+(43(+48<+89&+&)8&)&22:AD>A
@HWI-H225:69:CBJ27ACXX:8:1101:7527:2254 2:Y:0:AGTCAA
ACAGATCGGAAGAGCGTCGTGAAGGGAAAGAGTGTAGATATCGGTGGTCGCCGGATCATTAAAAAAAAAATAA
+
1*1**:?DDF1)9(/(77;5')-)782(6?>D?>((.;(.(-=>,8',,3900&&0&(:4(:44(5&&)&2(:
@HWI-H225:69:CBJ27ACXX:8:1101:7619:2284 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGGCGCCGGATCATTAAAAAAAATGACC
+
:@GGI4D?0;BGGCGGICE?D(6>A=@;A;CCACCD(;;>C:A?&59-&50<@&009(:@@A:@CB00((+((
@HWI-H225:69:CBJ27ACXX:8:1101:7727:2397 2:Y:0:AGTCAA
ATAGATCGCAAGAGCGTCGTGTAGGGAAAGAGAGTAGATATCGTTGGTTGCCAGAAGATTAAAAAAGACAAAC
+
00)9DF48(-7-@(3'-;4,,6((.,.5;(;(((,,(55(((,,((+(((++((((((::>(::@&&((39?(
@HWI-H225:69:CBJ27ACXX:8:1101:7627:2489 2:Y:0:AGTCAA
AGCTCCTTTTCCTTTTTCTTGTTTTAAAAATTCATTTTTTTTTTTGTCCCCGGTTAGGGGAAGTAAGAGATAT
+
)))0*00*?D*/)8)/)))/=)8)))))))6)))7?D7)3';'5>&+4>(8&&&&)2+8-9&2(+(+4(((+:
@HWI-H225:69:CBJ27ACXX:8:1101:7952:2254 2:N:0:AGTCAA
TCCTCCAGACGATCGGAAGAGCGTCGTGTAGGGAGGGTAGATCTCGGTGGTCGCCGTATCATTAAAAAATTAA
+
**11)999:)00((0;;1;(8=;'(,,'.6(;;;@'3,:((53>328+805+002-0&88:@@@>C:>>((+4
@HWI-H225:69:CBJ27ACXX:8:1101:7861:2261 2:Y:0:AGTCAA
CGCCAAAAGATTCAAACAGTTTTTATATCAAACTATACAAATCACTCGCCACGCCTTTCTGATTTACGTGCAC
+
1)8000))0(9***/)/.)/)/);(.@7).)=7))7))).).7).(.(,'('''((,,(((+(+(+(+83(((
@HWI-H225:69:CBJ27ACXX:8:1101:7992:2262 2:Y:0:AGTCAA
ACATTTCGAAAAAGAGCCGAGGATCGTCTAGGAGGACAAGGCGGTGGTGGTCGGTGGACGGAGAATTATAAAA
+
***009*??B16(@())(-'''')),..((-((-,5(59<A,&09&5&((&+&5&(&(+&)&&&+(++((+4>
@HWI-H225:69:CBJ27ACXX:8:1101:7763:2263 2:N:0:AGTCAA
GACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT
+
GGIIIDHICGGIIGIIIJDGIIIHAEEEF?BBBBA?CCD?BB???(:4:>@>>@@BD??B0:>9?5&0<<:@C
@HWI-H225:69:CBJ27ACXX:8:1101:7989:2280 2:Y:0:AGTCAA
TCGCTGGGAACAAGATCGGAAGTGCGTCGTGGGGGGGAAGAGGGAATATCTTGGGGGGCGGCGTATTATTAAA
+
')''''';ACAE;).).(,;;'(55;8/'&)&&055&05((+(&&((+((+(4>1&&&&0&&&&)(34(4(4>
@HWI-H225:69:CBJ27ACXX:8:1101:7803:2426 2:Y:0:AGTCAA
AGGGGTTCAATGCCCCCATTTGAGGGCTTTTTTTATTGTCATCATTATTAAAAATGCTAAAGTCACATAAAGT
+
0/*9@7@C;)=@;@DHBAC>C@DFEBAAACDDB;(5@(+(+4(+:@:@A>:A(:8:43:@>444@@C3>(4++
@HWI-H225:69:CBJ27ACXX:8:1101:7884:2441 2:N:0:AGTCAA
GCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA
+
EEHHIGGIJIHIGGGGGAGGGIACBBB?ABBF;BCACA(;(;;@CCCCAB58?3:90?5))8<CDD3>CCDD@
@HWI-H225:69:CBJ27ACXX:8:1101:8247:2269 2:Y:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAA
+
'=C;AGAH?>CB;>C@@CDDDD=B??B;?<778?AAB9?BB9<(:(4+4:>>:2<8<@9@59?0)0<<ACC>C
@HWI-H225:69:CBJ27ACXX:8:1101:8187:2285 2:Y:0:AGTCAA
TGCTCCAGAAGATCGGCAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTTGCCGTATCATTAAAAAA
+
DHHE4).)=B3877(''5'(.739,3',(9(:?(55(+3:>4>(+4>ACA?289>(8(4&&)(+((++(+4><
@HWI-H225:69:CBJ27ACXX:8:1101:8600:2348 2:Y:0:AGTCAA
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTTTCATTAAAAAAAAAAACTTTACG
+
AAF<)):):9:0?@.'.)8=(....7=.==7?37?;7(5,,=95'38'8'::3(+++4::-&)&&&&++4+((
@HWI-H225:69:CBJ27ACXX:8:1101:9194:2266 2:Y:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGCCGTATCATTAAAAAA
+
B@D9FGGD9*?BGDF>=/-=B.--;@5AC9?DF>>ACCC(55;(5((,5(,(('&+5><595?BA3+4@:A>9
@HWI-H225:69:CBJ27ACXX:8:1101:9166:2295 2:Y:0:AGTCAA
AATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAA
+
?86BBDDE=AE=GDH<55;:9B(6;ADCBBBC9(5:@C;>>CCB?220&405<<<<<23>:3>:C@>999>&&
@HWI-H225:69:CBJ27ACXX:8:1101:9011:2383 2:N:0:AGTCAA
GCTGCTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC
+
ADGGGHJGIG=?AHFE>@B2?>@ADBD>?<A@=BB-5<?@CDCDD139+:4>4:>3:@AD08>-455<0&)<<
@HWI-H225:69:CBJ27ACXX:8:1101:9178:2455 2:Y:0:AGTCAA
ATATATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGCTGGTCGCCTTTTCATTAACAATATTCAAT
+
0@?***0/6BF6=.)-;;'5=;(6;1;5;((,5(,;>;(:;(((+0-33&)+&)(++(+:4((+((((++(+(
@HWI-H225:69:CBJ27ACXX:8:1101:9280:2274 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
DHEIJJJJEGHGGIIB=FAB8CF;CA:AAB>>B>@AECC(95?1@@CAD<?8?@0>-085&0(+++::@CCCB
@HWI-H225:69:CBJ27ACXX:8:1101:9451:2287 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCCGGTCGTTAAAAAAATAATTA
+
'-5))>)(:CH/;2(,9;3',,8888(+2+(+3+(3:((+4(2<2<&&()09&&&)&&0982((+5&(+((++
@HWI-H225:69:CBJ27ACXX:8:1101:9363:2314 2:N:0:AGTCAA
CTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAA
+
GHGCHGJJGIHAHGGGGGJJJJAAGGIHHF<ABEEED?BDA>C5>@C@CC>C@BB8?B9@9<BD5<AADDD:A
@HWI-H225:69:CBJ27ACXX:8:1101:9493:2349 2:N:0:AGTCAA
TGTAGCGTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC
+
0??D?>AG1?B(7.;;FDH7;====;?CEDFDDDBC;>;?;3893:@@>AD8>9(::B<:4(::2?2850>9>
@HWI-H225:69:CBJ27ACXX:8:1101:9275:2387 2:Y:0:AGTCAA
TTTGACTAGCGGGCTGGAGGGGAAGAGGGGGGCCCTCGGGGGGAAGCGCGTCGTTAAGGAAGGGAGGCGTAGT
+
1)))0****))(--'''(.??/,,(5,(''&&&))(()&&)5&)&(+0-55&&&0(((+((((&())+&)&&(
@HWI-H225:69:CBJ27ACXX:8:1101:9518:2422 2:Y:0:AGTCAA
TTACACCAGTACCAAAATTTTAAGTTCCCCTTTATTGAAAAAGATTTGAACTTTTTTTATAACATAATTTTCT
+
<4())7);(.7)(;6(.5(-(-5;5((,(,55,,((5>4((+&(4(4>(((444(40&(((4:3@:>((4((+
@HWI-H225:69:CBJ27ACXX:8:1101:9785:2341 2:Y:0:AGTCAA
TGGGCCCAAGATCCCCAGTTGGGCGGTTTTGTTAATAGGATAATTATTAAAGGTCCCCCAGTCATTTAAAAAA
+
:):))00)?;(*/))(.(.)).'(-',32((((,(((((((4+(+(4:((((+(((((&)(4+>(((++>:4)
@HWI-H225:69:CBJ27ACXX:8:1101:9769:2382 2:Y:0:AGTCAA
TCTACGCTGCTTCCTGTGAACAAAAAACGGAAGAGCGTCGAGAAGGGAAAGATTGGAGGTCTCGGTGTTCGCC
+
1**0*)0886B4?*))).=@)8).(.'-'(,(,(((,',,',()9?<(22(((++((92+((+(+(2&(,2&)
@HWI-H225:69:CBJ27ACXX:8:1101:10258:2448 2:N:0:AGTCAA
CCAGAAAGCTTCCATTCCAACAAGCTGCAGAAGACAGTATCTGAACAACACCATTGACAGTATTTGCGTTGGT
+
G>GFHGE>CDAEC9AHEFEBDB?CCCEED>AC?ACCCCDDCDCD>@A?828<8>A:A34>>CCDD>@<<B9?2
@HWI-H225:69:CBJ27ACXX:8:1101:10602:2462 2:Y:0:AGTCAA
GGTAGTTCCTGAGCACACACAACTACAGAGGCAAGTGCAGGGAAAGATCGTTGAGCCAGGGGGTCCCCCTTTC
+
00**0?FD*0**9*?()(-'(((.)..)7)-((.5?((;>(',(((,(,(,(9<3>((+<--&)&((+&&+44
@HWI-H225:69:CBJ27ACXX:8:1101:10926:2326 2:N:0:AGTCAA
TTCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAA
+
))0?G9B;DBDF;FCGIGI@;;5=4=;@A>=(6;A3=--(5:;;:@C??,25&(09<55588(::4>>CCB@>
@HWI-H225:69:CBJ27ACXX:8:1101:10830:2333 2:Y:0:AGTCAA
GCAGGGGTCAACTTTAATACTTAGGTTGACCTCTTTTGGGTAAGGAAGAAATGCCCTCCTTTCCTGGTACGCC
+
'.=8=''--=@7;;B>;@(.;((5>5>A(-(,(,5,>(,'+((++>32<><4>C3(4<<?CCC:>3(4:(&&&
@HWI-H225:69:CBJ27ACXX:8:1101:10872:2345 2:N:0:AGTCAA
CAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAA
+
IIIDGHJJIJJIJII>GAGF686;;BC3;=AAA>C>CCD>:C>AB8?-&45<@@-)88:AC4(4ADD<><0&&
@HWI-H225:69:CBJ27ACXX:8:1101:10979:2414 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAGAAAA
+
@ABEHBDHGGG<CFGA;DE8<?;>@?>ABDDD(:@BCDAADA((2(&&(55?0&)8?>@>4:@CC@55&+(+(
@HWI-H225:69:CBJ27ACXX:8:1101:10862:2484 2:Y:0:AGTCAA
TTTGGGCCAGAGATCGGAAGAGCGTAGTGTAGGGAAAGGGGGTAGATTTCGGTGGTCGCCGTATCATTAAAAA
+
':=4''.5(((,,,:9?B8222()&+0(3+++:?(++((+&&&)244(+((02+5)+5&(0)&+2+>((++(:
@HWI-H225:69:CBJ27ACXX:8:1101:11101:2301 2:N:0:AGTCAA
TCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA
+
IJJJJJGIEHHIJDGIGIIIAH@ABAECED@BCC6>5>>>>@@>>>?B8?B;><@?<<9A?CCD:@CDDB<&0
@HWI-H225:69:CBJ27ACXX:8:1101:11220:2487 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGGAGATCTCGGTGGTCGCCGTATCATTAAAAACAAAAAAA
+
FE)B**9(-7(;(8;;5(-',(.6>A3,(=;;(3'35;9:@4?B2<5<CBB<@<3(2(4>(44:4&8+(++00
@HWI-H225:69:CBJ27ACXX:8:1101:11434:2253 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAAAAGAAA
+
D@GCHEHDIBFF9@CGAHFB'..6@BABDDDD::A@ACDDDD9?(299@@&89&)&51@>:CC>A@909&++(
@HWI-H225:69:CBJ27ACXX:8:1101:11863:2333 2:N:0:AGTCAA
CAGAAAGCTTCCATTCCAACAAGCTGCAGAAGACAGTATCTGAACAACACCATTGACAGTATTTGCGTTGGTG
+
<8==B<CG7@=@)@74==CE?E9??;@DDCE6;>CCCD@;C@AA:>?((882>C4>CCD@(>CD4>><B@<(8
@HWI-H225:69:CBJ27ACXX:8:1101:12125:2427 2:N:0:AGTCAA
GGGGGGGGAAAACGGGTTTTTTTTTTTTTTACGAAGAAAAAATTTCGTATGTTGAACATAGCTTTTATAAAAG
+
';BHHDB-07??35(6&2?B?&50&05-7/2((20(&3<3(&(+4+(((+(++((4(+(+((+(4:((++43(
@HWI-H225:69:CBJ27ACXX:8:1101:12341:2313 2:N:0:AGTCAA
GAGACACCCCGTTTCTCTGTATAGCTCTGGCTGTCCTGCAACTCACTCCGTAGACCAGGCTGGCCTCAAACTC
+
4(5@E>CCHGFBDE>ACEACCD335>;;>@><?A@AA>CC@C>3(94:<@?AB?>:38<AABB8?C@@>>>::
@HWI-H225:69:CBJ27ACXX:8:1101:12477:2365 2:Y:0:AGTCAA
GGGGGGGAAAACGCGGTTTTTTTTTTGTTACGAAGTAAATGATTCGTATGCTGTACATAGCTGTTATAAAAGT
+
';AAH6B0<?<>:6@@89BD(55&07&<C((+2<<?ACC@:4:>(+339((4(4:4:::::A(4>@@:(4>3(
@HWI-H225:69:CBJ27ACXX:8:1101:12487:2402 2:N:0:AGTCAA
TGCAGTTGCAAGATTTTTTTCTTTATTAAATTTAAAACTTAAAGGAAAGGGGAAGCCTTGTTACCTTTTTGGC
+
E')))7)).7)))).;B;3':(,(,,((,,,,((((4(((44((+(99(8?&5&(((4++:((((++(+(0&:
@HWI-H225:69:CBJ27ACXX:8:1101:12802:2455 2:N:0:AGTCAA
TGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA
+
??B=BGIGJGFHIGIHHGF>;=9??>@ACCCDCDA<AC:@>@CADDDD<B8<>9C<<B9&08?>ACCACDCB9
@HWI-H225:69:CBJ27ACXX:8:1101:13165:2320 2:Y:0:AGTCAA
ATATATCGGAGCAATGAATGCTGGAAAAAGACTAAAAGACCCACAAGGGGTTGAGTCCCTAATAATGTAATTA
+
C*1**1*)0))./)0?88/)))))..8.=;A;>3))..(..6((,(,5(','3(((+(+:(+((4(((((+:4
@HWI-H225:69:CBJ27ACXX:8:1101:13034:2330 2:N:0:AGTCAA
GAGGTTCAATGCCCCCATTTGAGGGCTTTTTTTATTGTCATCATTATTAAACATGCTAAAGTCACATACAGTG
+
I@DG@A>ECC>EEHFFCEEEEA?CDBABDDDDD0@C3>>ACCDDC>CDCDD:@@>ACCCD@:@@CC>C@C>:@
@HWI-H225:69:CBJ27ACXX:8:1101:13321:2352 2:Y:0:AGTCAA
TGCTCCAGAAGATCGAAAAAACGGCCACCACGGTCAGAAAGGAAAACAACGGGGACCCGGTTTTCTTCATCTA
+
1?F40?F<*****/((*--8'''(-''((6((',(,9(,((((,((+(++&&5-59(8&&)05<:@3((++(4
@HWI-H225:69:CBJ27ACXX:8:1101:13438:2446 2:N:0:AGTCAA
TATTTTGTTCAAAAGGCTGTCAGTTTCTTGTTTGCCTTGCACATCACTGTCGTAACTCTGAAGGTCCAGCAAC
+
GAFHDA@@=>?;3?EFDFFA@>;;@CCCD@ACDCCCAC(:(::@:ACC>CCBD?BBDCC3:A@C?>C>CC992
@HWI-H225:69:CBJ27ACXX:8:1101:13636:2325 2:Y:0:AGTCAA
AGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCT
+
HABFHIIHHIIJ1BCEEFCD?E1;@CBDDDDDB?ABCA>A>CCDDDDACDCDDADDDD>C@CCC@@CBDCCDC
@HWI-H225:69:CBJ27ACXX:8:1101:13687:2356 2:Y:0:AGTCAA
TTTGTTCTTGGTTCGGAAAGGGGTCATTTAGGGCAAGAGAGAAGTTCCCTTTGCCCGCGGGGTCAATAAAATG
+
'>'9.(6(((((,(3/(,'(((&&*(:3>3(:((8+(((+8((((((+4(+:((+(5&&)&09>84((44(((
@HWI-H225:69:CBJ27ACXX:8:1101:13545:2358 2:N:0:AGTCAA
CATAGTTCATTTGTCTTGTTGACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATC
+
@@@;)8B@4@HH477?EACH(7??C7;A.;>AA;?AB?CCBB<?;18(90<<0)+(4>@&05<+2(0(++4:(
@HWI-H225:69:CBJ27ACXX:8:1101:13593:2471 2:Y:0:AGTCAA
AGTAGCGTTGGTGGGTTCTGACTCAGATAGGAAGAGCGTCGCGTAGGGAAAGGGAGGATATCTAGGTGGTCGC
+
)00(0?67(-=5;(-:AED3).7?CAE3(;;3;AA;93=?>&&)&5?0(288(2(&&+((((((+++4()40)
@HWI-H225:69:CBJ27ACXX:8:1101:13548:2476 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGAAAAGGGAAAGAGTGTAAATCTCGGTGGTCGCCGTTTCATTAAAAAAAATCGCT
+
@D@E<BFBFGHBH23;;14'(('6;;;=A235((,5(;;>:>9<B@&0(&&8))&3:>>@3>A>(&)&(+(((
@HWI-H225:69:CBJ27ACXX:8:1101:14107:2302 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAATAAATA
+
@GHJJGIIGEEG@FEG/;A9=DECEDABDDD<:@CDDDEDECDB<?B9A9BB955??@4@@A:@C<@(+4((+
@HWI-H225:69:CBJ27ACXX:8:1101:14022:2350 2:Y:0:AGTCAA
GGTAGCGTTGGTGTGTTCTGACTCAGATCGGAAGGGCGTCGTGTGGGGAAAGAGGGTATATATCGGTGGTCCC
+
//.5(79,;'((,(59>5;>;(5>3+::32&(20(0<5&0553<(+)9<5?((+(0<9(++4++(58?&&+((
@HWI-H225:69:CBJ27ACXX:8:1101:14029:2497 2:Y:0:AGTCAA
TGCTGCAGAAAATCGGAAGAACGACGATTAGGGGAAGCATCTAGAACTCTTTGGACACCATATCATTAAAACA
+
:C*?*0:*0?0?<*0?(<'()8''5''((,.((','((,,(,5,;,>@+((4:((+(+((+((:4((+::>38
@HWI-H225:69:CBJ27ACXX:8:1101:14453:2259 2:Y:0:AGTCAA
GCCTCTGCTCCAGAAGATCGGAAGAGCGTCGTGTTGGGAAAGAGGGTAGATCTCGGTGGTCGCCGGAACATTA
+
6DCHB=EGGA>===;?));;A1,';(;;=827<8(+8-(+2<(9()(((((+(+(2(25&())85&5&&+(44
@HWI-H225:69:CBJ27ACXX:8:1101:14375:2414 2:N:0:AGTCAA
GTGGGGTGAGGCTGCCATACTACTCTATGATGCTTTGTGTGGGGTTTATTACTAACTCACTTGATGTTTGACT
+
'.)(---466;?2=15(-5(5((,(,5;;(((+3(3(4((20;-5)89:@((4:>>4::934((+(+>@((((
@HWI-H225:69:CBJ27ACXX:8:1101:14473:2483 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
JJJIJIJIJIGI9CHIHJH9?BACCDCDDDDD:@ADCDEDEDDD?BD9C<BD<09BBCCDACDDDBD@000&&
@HWI-H225:69:CBJ27ACXX:8:1101:14575:2300 2:Y:0:AGTCAA
GCCGTTGGGAACAAGATCGGAAGAACGTCGTGTGGGGAAAGAGTGGAGCGCTGGGGGGGCGCCGTTTTATTAC
+
'7@''-;2<(7(6.(6.;5;;='9((,,(&)0+(+5785?(+9+(((+(&&+&0(&&&&&&)0&)&+(((::(
@HWI-H225:69:CBJ27ACXX:8:1101:14515:2463 2:N:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCCGTATCATTAA
+
ADHEHGEF;;@D;;;>>;?@@@B??CD38<5;8<>C8>A<A39:A::+:>ADDBB&&0&0))8-&)883>@3@
@HWI-H225:69:CBJ27ACXX:8:1101:14916:2431 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
GGIIIIJGGIJI(=FGBHHBDBA@CDCBDBDC5>:@>CDDEDBD8?B9CB>B90<?ACDEDDDDD@@905.)0
@HWI-H225:69:CBJ27ACXX:8:1101:15158:2316 2:Y:0:AGTCAA
CTTGCCTCTGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA
+
FHIIGJIE@GIJIJGHGH9?DB;@@F@CFEHEEBBA=A>CBCDD??C(::@:@A@ACAB(250:95?>>@1((
@HWI-H225:69:CBJ27ACXX:8:1101:15098:2390 2:N:0:AGTCAA
TTCTGACTCAGATCGGAAGGGCGTCGTGTAGGGAAAGAGTGTAGATATCGGTGGTCGGCGCCACCTTAAAAAA
+
)0?**09*088B488'5(-(-5-,''',,5;58C(<5?(5::4++((+(+028&)(&)&)&5))(++>ACD:5
@HWI-H225:69:CBJ27ACXX:8:1101:15726:2307 2:N:0:AGTCAA
CTCGCTTTGAGCATAGTTCATTTGTCTTGTTGACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAA
+
;F;C@C(5@3?A=;@DCA@>?C@>AACE;AC9;;;A:@C(25>?8?(+<CCB@<@-88(09<>95+(:>@3<8
@HWI-H225:69:CBJ27ACXX:8:1101:15668:2486 2:N:0:AGTCAA
TGCTTTGACTTGTTTGCCCAGAGATCGGAAGAGCGTCGTGTGGGGAAAGAGGGTAGATCTCGGTGGTCGCCGT
+
I6=@EH9?9=C)7?)..;@3;;(5;(;;?B8?A9&)25552(25>8>B133+9<A<::(4+882()0350<0&
@HWI-H225:69:CBJ27ACXX:8:1101:15827:2300 2:Y:0:AGTCAA
CTTGAGAACCCACATGGGGGGAAGGGCAGGGAGGGAGAAAAGGGTGGGGGACGGGGTATTAATAAAAAAGAAA
+
33155((((('(((++(+<&)&&(2&((&)&())00&)(+((+(++&&)&&&)&05&)((++((+4@:&&(+3
@HWI-H225:69:CBJ27ACXX:8:1101:15842:2325 2:Y:0:AGTCAA
TGTATTGCTGCTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGC
+
B)00BBB9?9BBA?D?D)8=FEC@663@@AHE==(;38,;;,855:AB>A?<+329(3+(+(4:88(800:<&
@HWI-H225:69:CBJ27ACXX:8:1101:16056:2370 2:Y:0:AGTCAA
ATAGATAGAAAGACGGTCGTGCGGGGAGTGGGGGTAGATCTCAGATGTCGCCGGTTCCTTTAACATTTAACAA
+
'''./).8)7).))--'5>'9(,''0&&(+(2&&&)+:(+(++:(((+:A8(0-&)2(44(4((+(((+((44
@HWI-H225:69:CBJ27ACXX:8:1101:16064:2391 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGGCGTGTAGGGTAAGAGGGAAGATCTTGGTGGTCGGCGTATGATTAAAAATAGGTGGG
+
:?**0*0B)?G0?()<'-''''.6;=(;;((-(3'5<A>C(((4:?00(0&00)&((+(+4:A::1((+((2&
@HWI-H225:69:CBJ27ACXX:8:1101:16225:2436 2:N:0:AGTCAA
GCCTCTGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTCGCCGTATCATTA
+
<)88=EC@@;CC>CE>B@>?C7;'(((9=8'05++3:<3<28(3(5+(2:>(4(8<&095&&0<5&)22(:>>
@HWI-H225:69:CBJ27ACXX:8:1101:16400:2289 2:N:0:AGTCAA
CTATACCAATACTATTTTTTCCGCTTTCCCTTAAGGGAGTAAGATCTGTAGTTTCCATATAACATAATGGCTG
+
@F>F@@@CCE)7?>;CEHHC;(5'9?B>CCAC:>:(,938?>3>>:C:++:4@D@:3:4::@>ACCC@4@89?
@HWI-H225:69:CBJ27ACXX:8:1101:16295:2350 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAACAA
+
EHIIFICHJGJGGIIGBDE<>;;ACDCDDDDC:@@BCDDCDCB@8?<5>@>?9-0<<CAD3::AC9>50)&(+
@HWI-H225:69:CBJ27ACXX:8:1101:16505:2281 2:N:0:AGTCAA
TTTTGATGTATTTGTTTTTGTTGTTGTTTTGTTTTGTTTTTGTTTTTTCGAAACAGGGTTTCTCCGTATAGCC
+
':BB??@>BADBD;>CDDD?BD8AB<CBDD8ACDD0<CDDD-<CDDDD()2?@<C?BB3?B4>>@-<?BAD@C
@HWI-H225:69:CBJ27ACXX:8:1101:16676:2439 2:Y:0:AGTCAA
GTTGGCCCAGAGCTCGGAATAGCGTCCTGTTGGGAAAACGTGTAGATCTCGGTTGGCTCCGTATCCTTTAAAA
+
4*0)?*9BB(?2=))'--;(=.7-'5',(6(((35?B(,',,(,(5(+4((+2&&&(((+&28<8(+4(4>(4
@HWI-H225:69:CBJ27ACXX:8:1101:16721:2467 2:N:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTTGGTGGTCGCCGGATCCTTTA
+
H3=@AEEEF@DFEECCACD@B@B<2<=;<5&02<:>?(8(+33+4+4:(44>((:+28&+0-89&&))(4:((
@HWI-H225:69:CBJ27ACXX:8:1101:16859:2465 2:Y:0:AGTCAA
TCTTCTTTCACCCTTATTGTCCCAGCTTCCTTTTTTGTCAAAATTTCGAGTTCGGCGGTCGCCCTATCCTTAA
+
').)..))))).=())))(.(..((((5;(5(5,(3)((+(++4(43(0(++:(&&&&&&))2&)(+(((+(4
@HWI-H225:69:CBJ27ACXX:8:1101:17055:2284 2:Y:0:AGTCAA
ATAGATCGGAAGCGCGTCGGGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCCGGATCATTTAAGAGGGAAATG
+
H6?;4B<BF((-(-'5-=A'85,(8??2?253(+:3:>3((+(288<-30)895)5&+4>((((+(())(((+
@HWI-H225:69:CBJ27ACXX:8:1101:17035:2375 2:Y:0:AGTCAA
TCTTTGACTTGTTGGCCCAGAGTTCGGAAGAGCGGCGTGTAGGGGAAGAGGGTAGATCTCGGTGGACGCCGTA
+
/7.))))7??3?=;2@;=?=.5-;;=BDD89((0&&)&))(4><-5&2(829+38:>CC:20229((055&&)
@HWI-H225:69:CBJ27ACXX:8:1101:17079:2402 2:Y:0:AGTCAA
TGCTCCAGAAGACCGGAAGAGCGTAGCGGAGGGCAAGATTGTTGATCCCGGGGGTCTCCGTGTCATTAAAACC
+
000*9??D*8=8))-'5-4.;;4'(,('',3;3&8(2(((++(((+:(+2<&59&0((+5((+(((++44(+(
@HWI-H225:69:CBJ27ACXX:8:1101:17519:2334 2:N:0:AGTCAA
AAGCTCAGCAAACTTGCATGCTATGTGGGCTGTGTGACCATCCAGACCAGGAGCGTAGCCAGCACTGATTTGG
+
GG4@;;)=DCA7ACE9@CE7;B>@(66>@DD8?5@(5>55>C?@:3+2+2812<@0<@BDA8?C?@?CACD>:
@HWI-H225:69:CBJ27ACXX:8:1101:17904:2370 2:Y:0:AGTCAA
GGGATTTAGTTATTCTTTATTGAAACGGAGGGTGGAGTGCTGAAGAAAAAGGCAGATCGGAAAAGCGCGGGTA
+
@4;()77)7.7))77;B@);;(6;;(,,59'35<;<A+(+:3((4(+(985((++(((2&8?&3(+05<&&))
@HWI-H225:69:CBJ27ACXX:8:1101:17926:2378 2:Y:0:AGTCAA
TTAGTTGCCATGATTTTCTCCTTGATCAAGTTGAGAACTCAACGGAAAGGGAACGCTTGGTACTTGTTTGCCC
+
6'(..7)77;??;?BDE;;>3;>5>C@;A:AA3(:5>>>ACCA@B<29ABB?B@@><<2<4>>AC:@C(3(+>
@HWI-H225:69:CBJ27ACXX:8:1101:18099:2281 2:N:0:AGTCAA
TGTAGCCTTGGTGTGTTCTGCCTCAGATCGGAAGAGCGCCGTGTAGGGAAAGAGCAGAGATCTCGGTGGGTTC
+
7?DBFI)=BF9=(.-6=CEE;=A?;;);;(35=6;;38'3;&5((4:8(8(+3++(+(+(((+4<(2<0&&((
@HWI-H225:69:CBJ27ACXX:8:1101:18147:2301 2:N:0:AGTCAA
TGTATTGCTGCTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC
+
HIGGIIIJJJIJIHIJIIJG)BBDEEHGGGFFFCCA@B6?B=?ACCDD>AB???3>3>@AC@DA?B8<B0>@<
@HWI-H225:69:CBJ27ACXX:8:1101:18058:2426 2:Y:0:AGTCAA
CTCGCGATGTGACATGTTGGCCCAGAGATCGGAAGAGAATCGGGGAGGGAAAAAGGGGAGGTCTTATTGGTTG
+
*0*0B?(('.)8.)))//B2==(-==?3));=(5>,;((-((3''&)0)&2((()(+&&)&++(4((+((+((
@HWI-H225:69:CBJ27ACXX:8:1101:18201:2447 2:Y:0:AGTCAA
TCAGTTGCCATGCTTTTCTCCTTGATCAAGTTGAGAACTCAACGGAAAGGGAACGCTTGGTGCTTGTTGGCCC
+
6DCEGG=CHHHDHDDFF@@@CAA6;A;;@@CD;C@BC>CCCCC?B?+58?@AB8<B?B?B48>>@:8A(24>A
@HWI-H225:69:CBJ27ACXX:8:1101:18419:2284 2:Y:0:AGTCAA
GCTGGGGTGTTCCGACTAAGATCGTAAGGGCGACGAGTAGGGAAAGAGTGTAGAGCTCGGTGGTTGTTGTATA
+
.'7/4(5'-,;?)''3''-55(:9,(,((2()&))&)+(4>B(+219<44((+((((()5(2&5(+)(++4+(
@HWI-H225:69:CBJ27ACXX:8:1101:18414:2487 2:N:0:AGTCAA
ACAGACAGATTGGCTTTATTCCTGGACACACTGGAACAGCGGATAGCTCGGAAGAGCGTCGTGTAGGGAAAGA
+
=G>@DC2@)=A;();@@;@D>6.6@(5>;(9(5?AC((((&5&05944()59?&82<&085558(::((+2((
@HWI-H225:69:CBJ27ACXX:8:1101:18496:2491 2:Y:0:AGTCAA
GTCGGCGGGAGGGCGGTCGGGCGGGGGATGGGTGGAGAGATAAGGGGCCGCGGGGCCCGTAAAACCTGCCCGG
+
8)0).('-9',,'&&)&)7&)&&)&0&&&((+(&&22+(++((+(20&)))&0&&&&)&)&&+(((((+(+&&
@HWI-H225:69:CBJ27ACXX:8:1101:18928:2365 2:N:0:AGTCAA
TGTAATGTTTGCATCTGCTCCCGAAGATCGGAAGAGCGTCGTGCAGGGAAAGAGGGTAGATCTCGGTGGGCGC
+
:D8BE>8?BC8=C98)8@4=CGEAEAD?B=<?C@B(;3',,,0(+:<98?B19(()+((+4:@4(2(2<&(0)
@HWI-H225:69:CBJ27ACXX:8:1101:19107:2353 2:N:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
GIIGIIJIIJJJGGHA?D<;>==CABCDD?CC5@A>@A::ACBD?BD9@9@BB09A?AADCCDDD@B955&))
@HWI-H225:69:CBJ27ACXX:8:1101:19315:2307 2:Y:0:AGTCAA
ATAGATGGGAAGCGCGTCGTATAGGGAAAGAGCGGAGATCTCGGTGGGCGCCGTCTCATTAAAAAAAAAATGG
+
D@@*98*08)86;A'--=A/((-5>?>?A93933&&0&94(+(((+5&+509-&&+++:>3(+::5&)&&(((
@HWI-H225:69:CBJ27ACXX:8:1101:19438:2445 2:N:0:AGTCAA
CTTTTTGGAGGGGGAATTAGAGGGCGAGCCCTCTGGGGACAAGGGGCCACTGGTAATTGACTCGGTTTTGTCC
+
B@FHE@GG>>ED'3>'9@CD<(5;&&))5<((29:(97(08<AAB;3<?2:AA@4+(4:@+(+2?(008(+(+
@HWI-H225:69:CBJ27ACXX:8:1101:19501:2363 2:N:0:AGTCAA
TTGCGTTCTGACTCAGATCGAAAAAGCGTCGTGTAGGGGAAGAGGGGAGATCTCGGTGGGCGGCGGCTCATTT
+
0(0*-'75;4))).=3@@)6(),'6'(,,;'',((5,8&&0(+(+5&055(,:((82(&&+))0&&&0)+>:(
@HWI-H225:69:CBJ27ACXX:8:1101:19661:2393 2:Y:0:AGTCAA
TACCTTTCTCTCTCCTTTTGTAAGGTCATCCTTCCCTTATTCCTCTTGGGTATCTAAGTCCTCCCCCCACCTC
+
;@E>@@C77)7.;?)7;?;(((..5;((((555,55,:5>;555((+(+(+4:+:::44+:>((+5&)))&+2
@HWI-H225:69:CBJ27ACXX:8:1101:19980:2264 2:Y:0:AGTCAA
GGTAGCGTTGGCGTTTTCTGCCTCAGATCGGAAGAGCGACGCGTAGGGAAAGAGGGTAGATCTCGGTGGGCGC
+
0?(7(8@@F4=)5-,'5;@C>;;@C>CDC?B8?89558-55&5)0;?B(2<?39(0(+(+(+:@B@2<9&(&5
@HWI-H225:69:CBJ27ACXX:8:1101:19951:2308 2:Y:0:AGTCAA
TCCTCTGCTCCAGAAAATCGGCAGAGGGTCGGGGGGGGCAAGAGGGGGGATCTCGGGGGGTCCGGGAACCTTT
+
H9@47)7)..7)7)7)6(;;;>''(((,,(&&)&))8@&&((((29&&&&)+4>88599&&&(&)&)&((+:(
@HWI-H225:69:CBJ27ACXX:8:1101:19983:2353 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGGCGCCGTATCATTAAAAAA
+
GHIHIIBEHGIBB9?FCEEA@35--;4>6?BEF>;;>>=(3,55:,(55<?(29&+0)55&)28>:A:4>>AB
@HWI-H225:69:CBJ27ACXX:8:1101:19975:2375 2:N:0:AGTCAA
TTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCA
+
IIJJDGHGIIFGGFHIJIIIIGHFHGEFEEDABB?2<ACCDABBAAC+>4>:::(>ABB2<B-499@9)9ABD
@HWI-H225:69:CBJ27ACXX:8:1101:20086:2434 2:Y:0:AGTCAA
ATAGACAGGAAGCGCGTTTTGTAGGGTAAATGGTTATATTTTGGTTTGAGGTTTGTTTTTTTAAAAATTGTTG
+
+++++*1CG3))10?)?6'((.)))-))))).)).=).).;@>'6(((-,(-,((59995)&+(((4(+(+41
@HWI-H225:69:CBJ27ACXX:8:1101:20484:2269 2:Y:0:AGTCAA
ATAGATCAGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATACCGGGGATCACCGAATAAATTAAAAAAAAAAAA
+
0?)?9?B**0?**-;'5:'''.66.55(,(((,5,,,5((((&&&)&*2(+((&&+(4+((+:4:>&&059&5
@HWI-H225:69:CBJ27ACXX:8:1101:20647:2321 2:Y:0:AGTCAA
TCAAAGTGGAGATTGGAAGCGGGCCGTGCAGGGAAAGAAGGCAGACCTCGGCGGCCCCCGGCTCACTTAAAAA
+
/))).=)73).)....6(((,''')&&&&++()(((((((+((((((++20(&)&&&&)&&)05+(4+(4>@:
@HWI-H225:69:CBJ27ACXX:8:1101:20704:2383 2:N:0:AGTCAA
TGTAGCGTTGGTATGTTCTGCATCAGATCGGAAGAGCGACGTGTAGGGAAAGAGGGGAGATCTCGGTGGGGGG
+
6D(9<<---;;())..=).7))..7?@@7;<(;82;(3''3)8(+4(8(882(((*&)00((+(+22(0&&0)
@HWI-H225:69:CBJ27ACXX:8:1101:20556:2397 2:Y:0:AGTCAA
CATTTGTCTTGTTGGCTTGTAAAGCCAGAGGTCGGAAGCACAAAAAGTAAAAAAATAAGGTAGGTCTAGTGGG
+
*9***11?G??D*9)**((0**))/(8CG;().5'5('().((.,(35((((,35((+((++:(+(44(+(22
@HWI-H225:69:CBJ27ACXX:8:1101:20951:2351 2:Y:0:AGTCAA
GTCAGAATATTTAAGTTCCAACAGGCTGCGGAGGCCGGTATCTGAACACCCCCTTTACCCTTTTTTGGCTTGG
+
'().77=)))..).)..7(6>(((,((,,'',&&(+&&)&+(++>+:>(2&55&2:((4+(((+40)&(4(&(
@HWI-H225:69:CBJ27ACXX:8:1101:20931:2358 2:Y:0:AGTCAA
TCCAGAAAGCTTCCATTCGAAAAAGCTGCAGAAGATAGGTTTCTAAAAAAACCCTCGACCGTTTTTGCCTTGG
+
CC9****?*:9?BF**090*0('('.)8)8)).)))).)).77..7))(,',((((')&)&0232():+(,++
@HWI-H225:69:CBJ27ACXX:8:1101:21063:2371 2:Y:0:AGTCAA
CTTGCTCGGTAGAGCGCCGTGGCGGGCACGCGGGTTGACTTCCGGGGTCCCCCTTCCTCTTACAACTTCGGTT
+
,3/2,551&+(&((+&&)&)&&))))0&(&&)&&5(&(((((()&&&&&(++&&+((+((((+4(+((((&(0
@HWI-H225:69:CBJ27ACXX:8:1101:21083:2468 2:Y:0:AGTCAA
GACTTAGGGAAAAAAATTTGAATTATTGCGGTTTGGGGAGGAAATCAAACTTTTTTATTCACCAAACACTTTA
+
6-==).7;(.=;;(',;>(5((,(,,(((,')&&+8;5()&((((+((4(4(4>:&(+((+(+(2<(+((+((
@HWI-H225:69:CBJ27ACXX:8:1101:1543:2530 2:Y:0:AGTCAA
CTCTTGTTGACTTGTTGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCC
+
GIIBGBHIGFIEH<F@B;CB=;;(=;CGHEEHBBB3=>=>8?A?CC@5<A8AC4::@:>:>DCDD<25&0&00
@HWI-H225:69:CBJ27ACXX:8:1101:1833:2525 2:Y:0:AGTCAA
TGTATTGCTGCTTCCTGTGACCACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGC
+
?@B8?FHGIEGGACHIEHG=;7=CDEEHE=DBDBCAAB;??;@95>4<(8<<9?(&+(2:3:>CBD88>5350
@HWI-H225:69:CBJ27ACXX:8:1101:1983:2711 2:Y:0:AGTCAA
TTTTCCGTCTGTCTTGAAGGGCAATGGTGGGGGTGTGGGTGGGCGAAGGGTGATTAAAAAAAAAGCCCCCATC
+
0)?*0*)0((7.=4.=))))'(-(.)).7(','',,((&))0&()&&&))<8(+::@4>(-5)&&+++)0&(+
@HWI-H225:69:CBJ27ACXX:8:1101:2668:2730 2:N:0:AGTCAA
GGTAGCGTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGC
+
6D9*?>FHAAC@(6@6@CH)7)==AEEBDFBA?CBA?B;?8,0+9>@B>?AA3(()+>?C3:>:?@8?B.(&&
@HWI-H225:69:CBJ27ACXX:8:1101:2838:2508 2:N:0:AGTCAA
CTTCCGATCTGACTTGTTGGCCCAGAGATCGGAAGAGGGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCG
+
0???:D???FGG9@CABF;(.=CA66?===AD7;>6;(35('32<?CABAB?233(+(43:3:CABB<?9&(&
@HWI-H225:69:CBJ27ACXX:8:1101:2855:2652 2:N:0:AGTCAA
GGGATTTAGTTATTTTTTATTGAAACGGAGTGTGGAGTGCTGAAGACAAAGGCAGATCGGAAGAGCGTCGTGT
+
'5B=DGG<??E3?E);CC6@A(5@C;?@1;8<:@@:A:<@@@CC:>>ACCAC1<+2:9?@?@528(0)85))(
@HWI-H225:69:CBJ27ACXX:8:1101:2764:2715 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGAAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAACATTA
+
:@FCHIG;D@C;@>FE5;A5=)5@@C?BB=3;5>>,:;(5,5<?<@<-:9-<3&081>AD>CDCC9@0&8:+,
@HWI-H225:69:CBJ27ACXX:8:1101:3176:2608 2:N:0:AGTCAA
TGCACAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTTAAAAAAA
+
*111:CFGC@FB0B(06?=)75;;:6AC@BD@BC@;>;>A55@C:C>28<18;+5&8&&02833>(4>:(()5
@HWI-H225:69:CBJ27ACXX:8:1101:3122:2687 2:Y:0:AGTCAA
TCCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
0(738BFFGGGH94@A;C'=6)5==8@BBCAC?CDD?A9@::>4>>>>428889>A9<<&0&2+(4>44>:@<
@HWI-H225:69:CBJ27ACXX:8:1101:3036:2747 2:Y:0:AGTCAA
TCCGCTGGGAAACAAATCGGAAGAGCGTCCTGTAGGGAAAGAGGGTCGATTTCCGTTGCCGCCGTTTCATTAA
+
H)=(---55@(((;.,(5(',(',,('0<&2(+((383(8(3((&++)&((+4(2++2&(&&)&&)34(4:(+
@HWI-H225:69:CBJ27ACXX:8:1101:3279:2646 2:N:0:AGTCAA
GAGGGATTTAGTTATTCTTTATTGAAACGGAGTGTGGAGTGCTGAAGACAAAGGCAGATCGGAAGAGCGTCGT
+
I(;CC5@CE)7AC);C);BD(;>(;@CCDD7;(,5,<(9,2:@CCC3>39<C>A8<(2+(29<?5++())20&
@HWI-H225:69:CBJ27ACXX:8:1101:3491:2649 2:Y:0:AGTCAA
GGGATTTAGTTATTCTTTATTGAAAAGGAGTGTGGAGTGCTGAAGACAAAGGCAGATCGGAAGAGCGTCGTGT
+
0*9*?FFG<0B9*8/BFFBCG).8@).;6@ACHEEDBC;@;CAC<>ACDDC?9<?@A?A62<)(++)500)02
@HWI-H225:69:CBJ27ACXX:8:1101:3295:2706 2:N:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAC
+
6DEBE<FGD;B@(5AHEBC';(;>CB>BBB?CAC>CCAC@CDDD8?B0>@-?B9@B?DDD@CDDDB>B90)&)
@HWI-H225:69:CBJ27ACXX:8:1101:3570:2536 2:Y:0:AGTCAA
TGCGCTGGGAACACGCTCGGCCGAGTGCCGTGTAGGGAAAATGTGAAGATCACGGTGGGCCCCGTGTTCTTAA
+
;A7'',9/?23,55,','3/(05&0(+(()&0((++((++((++((+(,(((((&++&&++05&)&++(+4+4
@HWI-H225:69:CBJ27ACXX:8:1101:3650:2548 2:Y:0:AGTCAA
CCCTTGTCGCATAAACGCCCGCAGCAGAGCCGGGTAGGGGCATTCCACTGACCAGTCTGACGGATTCACTCAT
+
+11@E;C3))0)0**086?'---''6=)6.?'3>,'5(('',((((((++::A3(+(+4(+)5)&&+44(4(4
@HWI-H225:69:CBJ27ACXX:8:1101:3643:2624 2:Y:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
DFHAFGHGEIIEHIJJDGG;@A/BFH==?>@DE@ACBAC5>>>;>>@C:9?55=)+5<?<99<?>>C:@@CDB
@HWI-H225:69:CBJ27ACXX:8:1101:3908:2686 2:Y:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGAGCGTCGTGTAGCGTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTG
+
IEGIBEAHFFE7;C(.;;;;=<,(53888@57A+:@B7?>?28(++3(:@++:>C4>C9(50(&28(0)2<0)
@HWI-H225:69:CBJ27ACXX:8:1101:4132:2566 2:Y:0:AGTCAA
AAAGCTTCCGATGAAGAGACATCGGAAGAGCAACCTGGAGGGAAAGAGGGGAAGAAATTGGGTGAGCCCTTTG
+
)1*:F9***)))0?D*?(/.)))-';(5C(7).(.7@(?@B''(5((55''5&((3++4::12-2<(38?3>4
@HWI-H225:69:CBJ27ACXX:8:1101:4098:2596 2:Y:0:AGTCAA
TGTATTGCTGCTTCCTGTGAACACAGATCGGAAGAGCGCCGTGTAGGGAAAGAGTGTAGATTTCGGTGGGCGC
+
HG?BGFHB<B3BF@FGEH>CG=77=6A7?EBDBA;6>?'3;@@B9>CB9?<<9<+:4>C(4(++9<2<@&(5&
@HWI-H225:69:CBJ27ACXX:8:1101:4216:2688 2:Y:0:AGTCAA
TCCCAGAGATCGGAAGAGCGACGTGTAGGGAAAGACTGTAGATCTCAGTGGTCGCCGTTTCATTAAAAAATAT
+
./8)87=;7@=C/55,;?@A',,,5,(,:<(++(++(+(((4((((((+4(:4&&+55&+:+(44((:>0+4(
@HWI-H225:69:CBJ27ACXX:8:1101:4064:2697 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
0?FHGIJJIGIJGI)B@GH<>=@CECABB@CD>CADC>CCDDDD?@B5AB<B@BDDDDED@>ACC@05&&)&&
@HWI-H225:69:CBJ27ACXX:8:1101:4358:2563 2:Y:0:AGTCAA
TTTTCTGGCCTGAAGCACGGAAAGGAGGAGTGTTGTGTAGGGGAGGGGGGTAGGGTTGGGGGGGCGTCGTATA
+
0'-'.))((((((-((-('3813((+(0(2(+::+3((((+()&&)&)&&&&(((+(&5<---5&)0)&)(+:
@HWI-H225:69:CBJ27ACXX:8:1101:4254:2616 2:Y:0:AGTCAA
TATTCCGAGCTGCCACGCTGCCGATCGGTTCTTCTTGTCCCTTGTCCCAGAAGAACGTCGTATGGGGAAGAAA
+
'.)((..'3/,(,,((''&&)2&&&00&(&(+:(3((+(++(((+(((++2+(3(()(+&)(((((+(&+(((
@HWI-H225:69:CBJ27ACXX:8:1101:4670:2667 2:N:0:AGTCAA
GGTTTTTTGTTTGTTTGTTTTTTGTATTTTGAGAAAAGTCTCATTAGGACCCGGGCTTGCTGCTTCCTGTGAA
+
A/@;CGEF'6;A;@CCA=?CCBB2<(:>BC38?BDAD?44::@@@C(++>AB<@<9BB<8@@>CAAC>(4434
@HWI-H225:69:CBJ27ACXX:8:1101:4716:2723 2:N:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
6::BBDG@FCD@(5':5?A8;(5;>??B@?>?(:3>>CD>CABD8?<):95@@)9A8ADD>4>CDB050)50&
@HWI-H225:69:CBJ27ACXX:8:1101:4727:2738 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
A>DGCB@GGIBF@FEG@CE?D=3>CCA=AAAC,:5;>@>3>CBB<?>5>>9BB5-<<AAC:>CDD55050&59
@HWI-H225:69:CBJ27ACXX:8:1101:4891:2717 2:Y:0:AGTCAA
TTCCCTTGGAGACACAACCGGCGAGGGCCCTTTTTTGATAGGGAGGTGAGATGAGTGGGGGCGCGTTCATTAA
+
G/..=?37))).,((.((,'38&&&&&&+&(((,:5)(((((+()&((+(+(+(4((+20<&)00)02((+44
@HWI-H225:69:CBJ27ACXX:8:1101:5187:2607 2:Y:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAATA
+
<:D>FGHGEGGA('5==AB39=<>@@??BAC<>C>@:3>:>CBDD@B5@BBD&)0<ACCD@CDCD9>3&00((
@HWI-H225:69:CBJ27ACXX:8:1101:5159:2646 2:Y:0:AGTCAA
CCTCCGGAAAGGGGCGAGTGGGGGGAGAAAGGAAATCTCGGGGGGCGCCGGTTAACTTAAAAAAAAAAAAAAA
+
1)))10)0)0)(/.-''',(;638&))&((+(((+(4((+(0&00&)&&&&)&)+(,+(+44())&&05<<55
@HWI-H225:69:CBJ27ACXX:8:1101:5038:2677 2:Y:0:AGTCAA
TGTCCGGTCGTACCTCATATGGAGAGAGCGTTGTTTGGGGAAAGGGGGGAGCTCTGTGGGGGCCCCATTATTT
+
:*0**))0(''((-)).)..).)(.--,(',(',(((('',,,9(+&&&&&&+4((++(&&&)&&&((((++(
@HWI-H225:69:CBJ27ACXX:8:1101:5045:2696 2:Y:0:AGTCAA
TGACCCAAAGAGAGTCTTCTAGGGAAAGATTGGAAATGTCGGGGGTCGGCCAATAATTAAAAAATATGAATTC
+
*?F**)):))9))/09*/*=))8.8@;A>)))(..).).).,'',&095&&&+(((+4(::A(&((((+(((+
@HWI-H225:69:CBJ27ACXX:8:1101:5317:2559 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGCATCATTAAAAAACCAAAGA
+
:?6@F>F=?DF:(.8B@CBE667.;;@AB?;;(955<555;:5528)&4&5+0&&)0:@C:@CCC>&((((((
@HWI-H225:69:CBJ27ACXX:8:1101:5352:2600 2:Y:0:AGTCAA
TTTGGTCCAAAGATCGGACGCCCGTCGTGTAGGGAAAGGGTGTAGATCTCGGTGGGCGCCGTTTAATTAAAAA
+
;=?/'(.>@(-;;AC(2<'&0&&)585)52((+2(+22(82(&((+(++(+8((&&+&&&&)&((++:(+(44
@HWI-H225:69:CBJ27ACXX:8:1101:5403:2673 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAAAAAAGA
+
:6?(*/?D6<CA=FGB5?B'==ACCB;AA?9<,:,>>>>3;>2<225&405<0&)05<>C4:@CC5009&&)(
@HWI-H225:69:CBJ27ACXX:8:1101:5285:2679 2:Y:0:AGTCAA
TTCTCTGGGAATCATATTGGTTGTTGTTTTTTTTGTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
+
*::**?**))*)****00*).(('((..==5'3,',(,((((&0-;5&)05>9&055&555&&&55<9&59&&
@HWI-H225:69:CBJ27ACXX:8:1101:5509:2564 2:Y:0:AGTCAA
TCGATTGGCGCTTTCTTTGAGAACAGATCTGAAGCTCGGAGTGCACCGTTACAGTGAAGATATAGGCGGTCCC
+
1*)))0))*(07A()/))))(..))).?A.).77)).'''',((,((,5(((++((+4+4((((+((005@5(
@HWI-H225:69:CBJ27ACXX:8:1101:5908:2563 2:Y:0:AGTCAA
TGCTGATTAACCTTTTTTATTTGTTTTTTTTCAAATTTAAATTAATATTTTTTTACAGAATGAAATTTCTTCT
+
:<?B@******0***9?'()../)).81=/'((-(((((((((((((((5(59-(+4(+(4(((((:4+::((
@HWI-H225:69:CBJ27ACXX:8:1101:5775:2728 2:N:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAACCGAA
+
)0?*/?F=FFH8(-55,5>B/(5(5<(9?9?95:3>>@3:>CBD(20&+9@B5<582(:>44>::&))&(&&&
@HWI-H225:69:CBJ27ACXX:8:1101:6206:2520 2:Y:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
HCE<FHGIGHAFAHGDHGAFH;;A@B::(7?CDAECB>A5;5>;;@>>>BB(8;0:99?-)0<?ADD>CCCDD
@HWI-H225:69:CBJ27ACXX:8:1101:6200:2542 2:Y:0:AGTCAA
TCGGAAGAGCGTCGGGTAGAACTCGGTGGGCGCCGTATTATTAAAACAAAATAAGGCTCTAATAATTAACTAA
+
?F=@6B6?09?6/<';(;5;).7)5?(59'(3';'&0((4:+4:4:31(((+(((+3(8((+44(+(+4(+(+
@HWI-H225:69:CBJ27ACXX:8:1101:6557:2503 2:Y:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAA
+
@CHGIFEEED;;AC;6;ABB@D@@A?B/<@7;<9@CD>AA?39(++4:>>:ACD<2<<&+058005AD<AD:@
@HWI-H225:69:CBJ27ACXX:8:1101:6637:2643 2:N:0:AGTCAA
AGTCCTCAGCAGGCTTTGCAAGCCTTAAACGGTCTTCCTGGTGGACTGGGAGTCAAGGGCAGAGCTAGACAGA
+
()/.8=4).=C(('.=)=CC>7((7(.6;>===;?C:@>@:>(5<:@B28AB@A:3>C@@(0(+9>@>>+((3
@HWI-H225:69:CBJ27ACXX:8:1101:6571:2650 2:N:0:AGTCAA
TCCGCTGGGAACCAAATCGGCCGAGCGTCGTGTAGGGAAAGAGGGTGGATCTCGGGGGTCGCCGGATCATAAA
+
)09?:DAHAEF5;@(6=AEE',3'3,8,570;B?A(8>(+2+9(0(+0+44(+88&55&)55<0&05&(4(+4
@HWI-H225:69:CBJ27ACXX:8:1101:6620:2662 2:Y:0:AGTCAA
TTCGGTTATGACGATATCTACGCTTAAACCTGACTTCCTGGGGTCCAGACTGGGACTGGTCACCCAGGTTTGA
+
1**1100)**9*)0?7'))/.;;((-7=?;=()7?);>>;((',',(((,,:((9((((+(((+2(+<4>:2(
@HWI-H225:69:CBJ27ACXX:8:1101:6524:2670 2:Y:0:AGTCAA
GCTAGAGTGTAAATCTCGGCGGGCGCCCTTTGTTTAAAAAAAGAGGCAAAAGTTCGGTGGTGGCCGGTCTCTG
+
)))0)**00?<*BG4/*/(('''')&&&)(+(++93((+450&(+(((+8C((((+5++9&+(+2)5&&)(((
@HWI-H225:69:CBJ27ACXX:8:1101:6579:2672 2:Y:0:AGTCAA
GCCGCTCCCACCATTAGGGAAATAGCAAACTTCAGGCCCCGGGGAGTGAAATGGATAAGCCTCCCACTGTGAA
+
))08(-'-(8.'()))))()(6.).))(.(((-55(,;1;'')0&&((+44>4::@((((((+(2(+((+>3:
@HWI-H225:69:CBJ27ACXX:8:1101:6682:2672 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGAACTCGGTGGTCGCCGTATCATTAAAAAAAACTTAA
+
))8?D@BF<;;FH9;'5;4BDB@>@?9=?(5,(,>5(5((5((5?00)>&5?&5028:4>@C@C><>@&(4(+
@HWI-H225:69:CBJ27ACXX:8:1101:6621:2692 2:N:0:AGTCAA
GAAGCTTTTTAAAAAGACATTTCATGTCTTTCCCCATGAATAAACGCATCTTGACAGTTTTTCTTTGGGGCAG
+
61@))))=DH2777.'5.((6;;66(5(;AD(((,(,(:@(;@(5(&&20+:(+(4+(4>CB(:((((00&)0
@HWI-H225:69:CBJ27ACXX:8:1101:6535:2718 2:Y:0:AGTCAA
TCGGAAGAGCGTCGTGGAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAGAATGGAAAA
+
09))08(((9@7@8/@';(,9';A@CC-;@>A;55>@(8?B:&+&5<59<B8A4>CC@>:5&&&+2(+(+(((
@HWI-H225:69:CBJ27ACXX:8:1101:6933:2691 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATAA
+
IJJEIIJJJFJJIJJJIJHEF?AACCEDDACD:A@B@CACDCDDBBD0>>@B909ABCCD4>ACD>>900(++
@HWI-H225:69:CBJ27ACXX:8:1101:7201:2625 2:Y:0:AGTCAA
GGTTGCGATATTGGGTTGTACATCAGATGGGAAGAGAGTCGTAAAGGAATGGAGAATCCACACCGGGGGTACC
+
))0)(/-'('')).6--'-.))))7;;((.(.((,5;,(,3((((,+(3(((++((((+(((+(&&&090)(+
@HWI-H225:69:CBJ27ACXX:8:1101:7000:2682 2:N:0:AGTCAA
CCAGAAAGCTTCCATTCCAACCAGCTGCAGAAGACAGTATCTGAACCACACCATTGACAGTATTTGCGTTGGT
+
;8))7@E77@===;?E>C;?C456=A3;;5>C3>?99,5>:;>CA:2<(8281::(4:::@@>BC((59?B?(
@HWI-H225:69:CBJ27ACXX:8:1101:7412:2643 2:Y:0:AGTCAA
TCGCAAGTTCGTCGTGTTGGGCAAGAGTGTACATTTTTAAGGAAACCGTATCATTAAAAAACTTTTGAACTTC
+
<+)))1)***)00)8(8(.8'()(()(..).).))7))()).).(.;'5(((5(,5>5,><)(+4:(((((+(
@HWI-H225:69:CBJ27ACXX:8:1101:7642:2504 2:N:0:AGTCAA
GGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATTTCGGTGGTCGCCGTATCATTAAAAAAAA
+
CFDHHGBB)??B)7?;A>=>FF;8:BD;)7;(6;>?(-(>;(5,(;@38?15&(&089&)2(4>4:ACCC00&
@HWI-H225:69:CBJ27ACXX:8:1101:7602:2552 2:Y:0:AGTCAA
TGTTGCGATTGTTTTGTCTGACTTTGATAAAAAAAACGTTGGGAAGGGAATGAATTTATATTTGGGTGGGTGC
+
0)0))*0(82;))).(.===;)7))7.).)..7'9','559(&(+(+0<+(+4(4(34(4::++(5<>&)0&(
@HWI-H225:69:CBJ27ACXX:8:1101:7599:2580 2:Y:0:AGTCAA
TGTAGCGTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC
+
9*009?F:BFG6?.;.B=@FGGGGGGEG@;BA?BDECD;BB;@?AACB3?A893(4::4(:@>AB??<@9>.&
@HWI-H225:69:CBJ27ACXX:8:1101:7563:2608 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAC
+
GEHEGFHGGGJJIJIG8@B9@=>@CCCAB@?C5@:B>ADCDDDB<?B5><>@9&5A@CCD:CCDDDB55&&&&
@HWI-H225:69:CBJ27ACXX:8:1101:7529:2672 2:Y:0:AGTCAA
TTGCCTCATATCGGGATCGGGTCGTGGTCGGGAAGGGGGTGGATGTCGGTGGTGGGCGGATCCTTAAAAATAA
+
(+(((((,(+(++)&65+50&(&&&0&++57&078+5&&&&&(+(+(2-)20)(+&()&*0)((+((44+(++
@HWI-H225:69:CBJ27ACXX:8:1101:7673:2678 2:Y:0:AGTCAA
TTTTCCCTTTCTATGTTTTTAAACATTTCCTTAAAGGGGCAAGTTGGGAAATTTTCGAGATACCGAAGGGTTC
+
('-5==).).))))).?.6((((-(((-;(,,(((((('))++(+(+5192(+(+(&((((+++&&&&)&(((
@HWI-H225:69:CBJ27ACXX:8:1101:7918:2541 2:N:0:AGTCAA
CTTGTCTTTGCCGCTGGGAACAAGATCGGAAGGGCGGCGTGTAGGGAAAGGGGGTAGATCTCGGTGGTCGCCG
+
*:0*0::?9***.@<FB;8@@()..77?<B=B(,(3'05)59?CC@388<(2&0)5<::+>3228(&&+)&20
@HWI-H225:69:CBJ27ACXX:8:1101:7856:2612 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCCGTATCATTAAAAAAAAAAATG
+
:)B0DHIB'BB;>()--5559(.6@A?A=;>9@5@>:3@:5:A7&09&)008&9)2<(:C(4(+4&5>@.08(
@HWI-H225:69:CBJ27ACXX:8:1101:7820:2663 2:Y:0:AGTCAA
TTTAGGGAATTAACGTGGACTCATTTCAACTACAGAGCCTCGAAAAAGTCCTGTATTGTTATTTTTCGTCACT
+
D6)8*9DB8(*9**-.(-@3))..).)))))).).))7;C('3?B/(,(,55:ACDED;:::CDDD3)2(++4
@HWI-H225:69:CBJ27ACXX:8:1101:7842:2740 2:N:0:AGTCAA
TGTAATGTTTGCCTCTGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC
+
?@?BGHIFEGBHGCFECGGHICGEIEFE:EDFF>A>>>?B?8=9<ACD9<A?93(+443:((:48?8?B5405
@HWI-H225:69:CBJ27ACXX:8:1101:8058:2646 2:N:0:AGTCAA
GGCACAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAA
+
8CF*?DGG30*?;)/?6.BF''5@5AAACDF@E?;;>(,,-85@3@:?B<?B0(9-89)0<?(:>(+>AA&00
@HWI-H225:69:CBJ27ACXX:8:1101:8163:2688 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAATAA
+
I8GGGGGF<FGGHIDI;DE4??>>CDEDDBCC:@A@CCD@C@?8<850@9>?5)5A14:C4>:CC@B909((+
@HWI-H225:69:CBJ27ACXX:8:1101:8224:2719 2:Y:0:AGTCAA
AGGGATTTAGTTATTCTTTATTGAAACGGAGTGGGGAGTGCTGAAGACAAAGGCAGATCGGAAGAGCGTCGTG
+
67@F;C=C94)7=C=;AC;);B37>CE?((,,;(92,022:@CA>A>?CC3+988<@:929?@&823<029.)
@HWI-H225:69:CBJ27ACXX:8:1101:8422:2521 2:Y:0:AGTCAA
CTGACTCAGATCGGAAGAGCGTCGGGTAGGGAAAGAGGGTAGATTTCGGTGGTGGCCGTATCATTAAAAAAAA
+
?;?4<*::39?9D@@@;(..-'('',('5=??A;;3<(85(((;,54?>8<5)(+22&)&++(4>C@ACC@5&
@HWI-H225:69:CBJ27ACXX:8:1101:8490:2585 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTTTCATTAAAAAATTTTTTC
+
?:@FHI>DB7;686@6BCBB/@);@A(.;;3=55>;@3;@@C9<?8>9:9-?BB&3>4>>++(+>&(++4+)(
@HWI-H225:69:CBJ27ACXX:8:1101:8268:2664 2:Y:0:AGTCAA
AGAGATCGGAAGAGCGCCGTTCAGTGAAAGAGTGTCGAAATCGGTGGTCACCTTATGATTTAAAAAAGAAGAC
+
6'-(-77=5;B6(((,',','((,,,(((((3+++(&2((((()++&5(++4++(4((:(((((4()&((+(3
@HWI-H225:69:CBJ27ACXX:8:1101:8392:2718 2:Y:0:AGTCAA
TGCTGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAA
+
JGHJJIIIJIIIIEGFDHII:C@BAEEEEB?@DBCC(;6>>ADCDDBD8?B?AB>B<05A?3>C3@ACDDB90
@HWI-H225:69:CBJ27ACXX:8:1101:8635:2551 2:Y:0:AGTCAA
GGGATTTAGTTTTTCTTTATTTAAACGGGGTGGGGGGTGCTGAAGAAAAAGGGAGATCGGGAAAGCGGCGGGG
+
EGD(===>C=7)7;);?B).?)6;>@?B88'3'05&8&&((+(4(((+310?(+&03320&0&(((&&))0.&
@HWI-H225:69:CBJ27ACXX:8:1101:8694:2630 2:Y:0:AGTCAA
CATAGTTCATTTGTCTTGTTGACTTGTTGGCCCAGAGATCGGAAGAGCGTCGGGTAGGGAAAGAGGGGAGATC
+
;FG9)8=@477=C@;A;77?>>>?@?@@((.6==;((9::92<?018(0)2<&5(&(28988233()&0..((
@HWI-H225:69:CBJ27ACXX:8:1101:8539:2679 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
GIJIJJIJJIJGIJJI@EH?B?;CCD@BDBCC5>:B@CCCDCDD8<B5:<>B@50?BDEE:ACDC99955&&)
@HWI-H225:69:CBJ27ACXX:8:1101:8751:2508 2:Y:0:AGTCAA
TGTTTTTTGTTTTACCGGGCACGGCTTGGCCCCTCCCCCCCCGAGACCTGCCTGCCCCCCCCTCAACCCCGCC
+
=).=8)5''(...7;('''',8,20)0&<+(20(&(+0&&&)&0)&)+:(+8(++8(+)&0&&&((((&&&&0
@HWI-H225:69:CBJ27ACXX:8:1101:8804:2515 2:Y:0:AGTCAA
CGCCGCATGGCCTCAGGGCGACAGAGCGGCCCCGGGGGGGGCAGGGAAGGAATCGGGAGCCGCGGGGTCCCCG
+
0)08D?'7;=(8).)==((',',9?(;=',&&&0&)005&&&&)5;8((+((+((&&&)5(&&)&)&))8((&
@HWI-H225:69:CBJ27ACXX:8:1101:8933:2554 2:N:0:AGTCAA
ATATCACAATAAAAGTATACCTCGGCCAGGTGCAGTGGCTCATGCCTGTAATCCCAATACTATGGGAGGCCAA
+
GGIIJIGHHEHGIIIHIJGGEGDDDFABHCFHHGHIIHIGFCEEFFFFECDEEEECECDCCDFCDDDDDBDB@
@HWI-H225:69:CBJ27ACXX:8:1101:8983:2570 2:Y:0:AGTCAA
TTCGCTGGGAACAAAATCGGAAGAGCGTCGGTTAGGGAAAGAAAGTAGATCTCGGTGGGCGCCTTATCTTTAA
+
**/7(<::12)-=C((7)-=@;5((.,',3',3&+<A9288>(+(((((+(++(2(+&&(*&&&)(+((:@(4
@HWI-H225:69:CBJ27ACXX:8:1101:8947:2571 2:Y:0:AGTCAA
CTCGCTGTGAGCATGGTTCATTTGTCTTGTTGACTTGTTGGCCCAGAGATCGGAAGAACGACGGGTAGGGAAA
+
./8;((-'.))7).)..;)).66(((.;(5>((-;;3:>(,,:<(((((>(89??&8(3&&&)&0&)(+8(8<
@HWI-H225:69:CBJ27ACXX:8:1101:8817:2599 2:N:0:AGTCAA
ATAGATCGGAAGCGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
IGIIGIIJIIE=('5>>?B8;(9>9?98883<:>>:AC>::>?B2<>3:9>B3&)88:@C:CC:CBBB<9)&)
@HWI-H225:69:CBJ27ACXX:8:1101:8829:2613 2:Y:0:AGTCAA
AAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGGCGCCGTATCATTAAAAAAAAAAAAA
+
))00/*?8@BF/.()5-5',5..;@@3=A55((3,5(555(+(8&)5&&&)5&&&++(4>(:ACDDD@B555)
@HWI-H225:69:CBJ27ACXX:8:1101:8941:2665 2:Y:0:AGTCAA
GCTTCCGCACAAGAGGTCGGAACAGCCATCTTGTGGGGCAGAGGGTTAGTCCTGGGGGGGGGCGGGTCCCTAA
+
):*0**).(-'((8(().'-'-'...(.(.((((-(28'8&(+(&(+++((((+9855-&)0&&&&&)(((++
@HWI-H225:69:CBJ27ACXX:8:1101:8767:2665 2:Y:0:AGTCAA
TCCTCTATAAATCCGGAAGAGCGGTCTGCCGGCCAAGCGTGTATATAGGGGTGGTCGCCGTCCCCTTAAAAAC
+
78CB8)=))88)))5B'''(.;33'''(,(''&&)(((++&*(((++((())&&&4)&(&))&)((+((+((&
@HWI-H225:69:CBJ27ACXX:8:1101:8782:2681 2:Y:0:AGTCAA
ACCTTTCTCTCTCCTTTTGTTAGGTCATCCTTCCCTTCTTCCTCTTGGGTATCTAAGTCCTCCCCACACGTCC
+
(/=).8)7.).).).7=A7..;;;>A6;AC:ACCA(-((5>:5>5:::8(+44>34:(+::ACBD<<08&(28
@HWI-H225:69:CBJ27ACXX:8:1101:8785:2743 2:N:0:AGTCAA
TTTGCTGTGAGCATAGTTCATTTGTCTTGTTGACTTGTTGGCCCAGAGATCGGAAGAGCGGCGGGTAGGGAAA
+
'>',.;(.((((-((-(-(,5@>;,(5:5,:5,:+>(4>28(+8++((((+258@&2((0&)0&))008?(2?
@HWI-H225:69:CBJ27ACXX:8:1101:9108:2501 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAC
+
GEHCGBHIGIGGFGBHGGHBB?>@CCABDBCC,:AD@;@CDDBD(8>-:9B?599?ACDDACACDBB95.&)&
@HWI-H225:69:CBJ27ACXX:8:1101:9098:2614 2:Y:0:AGTCAA
GTTCCCGTTTGTATTTTTTGACCCAAAAAGGGAAAACGTCGTTTTGGGAAAAAAAATAGAACTCGGTGGTTGC
+
',3((.,;2(,9(,(,5(&&(3(++(+(+&++2(((()++&0&(+(&)(3?9()&&(+(+((+((((+5)+++
@HWI-H225:69:CBJ27ACXX:8:1101:9104:2676 2:Y:0:AGTCAA
CTGCCCCCCACTCGCGTCCCGCTCGGTCAACCACCAAATCTCGCTGTCCCCAGGCCCAAGCCGTCCCATCCCT
+
)0*)0(/''''(.--5',,''',,,&(&)((+(++2(2:((4(2+&)(44+(((+((2(+((&&)&&((+(8(
@HWI-H225:69:CBJ27ACXX:8:1101:9470:2722 2:N:0:AGTCAA
AAGCTCAGCAAACTTGCATGCTATGTGGGCTGTGTGACAATCCAGAACAGGAGCGTAGCCAGCACTGATTTGG
+
IEC>AHIJIIIHGFFFFFDFFFCEACCCDBDBBACBDC@CCDDCCDDCDCABBBD<BDDDC@BDBDCDDDEAC
@HWI-H225:69:CBJ27ACXX:8:1101:9852:2557 2:Y:0:AGTCAA
TGGCCCAGAGATCGGAAGAGAGTCGTGTAGGGAAAGAGGGTAGATCTCGGGGGTTGCCGGATCATTAAAAAAA
+
DB?).872(6.7=@B5AE>6)57;358(;AC7((,55<(8(+((++:(28&)-)))+49&&&0(::3:A>A95
@HWI-H225:69:CBJ27ACXX:8:1101:9754:2600 2:Y:0:AGTCAA
GCCTGCCACAGGACGCACGGAAGAGAGTCTTTTATGCACAGTTTGAAGCTCTTAAACTGTGGAAAAGCAAACA
+
)))0(/9*).(.8('''-'',('.5((-;>;((,55@D(5(,544>(+(((+(+(++((+++4((+(+993(2
@HWI-H225:69:CBJ27ACXX:8:1101:10032:2606 2:Y:0:AGTCAA
ATAGATCGGAAGGGCGACGTGTGGGGAAAGAGTGTAGATCTCGGTGGTCGCCGGCTCCTTTAAAAAAAGTAAA
+
00)??*/?A(-;('--',',,-(5/'(',<3<:(3((44@>:122?50(5-<&&&&)((+(43((:))&+(+4
@HWI-H225:69:CBJ27ACXX:8:1101:10114:2711 2:Y:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
HIDFGC*9<*?DDGGGD=GG2=;;FEEE=@BDF@@;@C=5;5-;5;@@@<???B5:>>@9&088:@A>>(:A>
@HWI-H225:69:CBJ27ACXX:8:1101:10156:2719 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAATAAA
+
:FBGAHGGE;BAGIIB@DHBEEEACDCB@BCC>AA@CDAADCBB<@>5>@3<@99A?ACDCCCDDD<5&(+(+
@HWI-H225:69:CBJ27ACXX:8:1101:10027:2731 2:N:0:AGTCAA
GGTTTTTCGAGCCAGGGTTTCTCTGTGTAGTCCTGGCTGTCCTGGACCTCCCTTTGTAGACCAGGCTGGCCTT
+
HA;CDHE)'-)',6>98?AB5;35:(,:;:5;,5>>13(2@>:>9A(+3(((3>>(((+(+:3?<<?8<8(:>
@HWI-H225:69:CBJ27ACXX:8:1101:10954:2516 2:N:0:AGTCAA
TGGCCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCGTATCATTAAAAAAA
+
G(?=FHICHIIGGIJCA=@@CD8=;;=?<:@@?AAAAC(4+44@4(:>??88<&()05905<?@:>CCDDDB9
@HWI-H225:69:CBJ27ACXX:8:1101:10922:2533 2:N:0:AGTCAA
ACAGACAGATTGGCTTTATTCATGGACACACTGGAACAGCGGATAGATCGGAAGAGCGTCGGGTAGGGAAAGA
+
===;..=3=DHEE<EDB9@D));>@666;;6?AA?A5539350)0(4>A?>B?52++55?@&)202?B>A?(+
@HWI-H225:69:CBJ27ACXX:8:1101:10850:2555 2:Y:0:AGTCAA
CTTTCTCTCTAGATCGTCGTGTAGGAAAAGAATGTAGATCTCGGTGGTCGCCGGATCTTTAAAAAAAAAAAGA
+
)0)*******0***08..''5..))))).7A()..7@)..(..(,;>'(3=5&&&)&+4>(+++:9@<@&&&(
@HWI-H225:69:CBJ27ACXX:8:1101:10917:2690 2:Y:0:AGTCAA
TGTAGCGTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTTGC
+
8???F<F@6@G5((5=A?CACE=@?B;@>AC7=>55(38?B;9+(4:<38?13<()(+(4(4>42(229<(22
@HWI-H225:69:CBJ27ACXX:8:1101:11216:2545 2:N:0:AGTCAA
TCTTCCGATCTGACTCAGAGTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA
+
DDGEBF6@GHIIBEGICFGH=C@G(;6@?EEHHFFFFFFECCCDDDD=?AB8?B>BBACAB9?<?><+:(44:
@HWI-H225:69:CBJ27ACXX:8:1101:11004:2643 2:Y:0:AGTCAA
GCTGTTTCCTGTGAACACAGATCGGAAGCGCGTTGTGGAGGGAAAGAGTGGATATTTCGGTGGGCCCCGTCTT
+
)*****0*/9B8))//=.B(==.6-(59(499,',5(((,20)(28(34((4(+4((++0+(&&2(&&&)(+(
@HWI-H225:69:CBJ27ACXX:8:1101:11289:2517 2:Y:0:AGTCAA
ACAGACAGATTGGATTTATTCATGGACCCACTGGAACAGCGGATAGATCGGAAAAGCGGCGTGTAGGGAAAGA
+
BBC>)=D@3=@CE..)=3)=?>;B3;@(56;=>A(55((58;&&&++4:()8<&(+(5-&&&)0+(4?C1?1,
@HWI-H225:69:CBJ27ACXX:8:1101:11414:2527 2:Y:0:AGTCAA
AAGATCAGCAAACTTGCATTTTATATGGGCTGTGGGGCAATCCAGAACAAGAGCGGAGCAAGGACTGATTTGG
+
=)=..)).)77)(7?;((666>(5((-(=(9;,5(8&)02>:<>3((3((((+3&&0&&(+((2+++(4>A3(
@HWI-H225:69:CBJ27ACXX:8:1101:11264:2633 2:Y:0:AGTCAA
TGCATTGCTGCTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGGAGATCTCGGTGGTCGG
+
-7(..=@9=@7))).7?7=;>C@C?>66@58?=895(8'5;&)(+,:?(28<(3++(+++(+:(2(+8<549&
@HWI-H225:69:CBJ27ACXX:8:1101:11524:2671 2:Y:0:AGTCAA
ATAGATAGGAAGAGCGTCGTGAAGGGAGGGGAAGATCTCGGGGGGCGCCCGATTATTAAAAAAAAATAGTACA
+
))898**0?*888=)-'(---),.;A(''',''((((+4?7&&&&&59>-&0-(2:((::>:0&)&((((((+
@HWI-H225:69:CBJ27ACXX:8:1101:11980:2509 2:N:0:AGTCAA
GCTGCTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATC
+
GHIGGIIJIGEEFEHEE?D@B>>CDB@8=<<@?@<5823>CB?AA<>A(4(44:::@AB@2?@-:9<B>)5<8
@HWI-H225:69:CBJ27ACXX:8:1101:11971:2603 2:N:0:AGTCAA
AAGCTCAGCAAACTTGCATGCTATGTGGGCTGTGGGACAATCCAGAACAGGAGCGGAGCCCGCACTGATTTTG
+
2C/)).7)7.=C9=C93;;;;>(....;?,(,,((',(,9(534((3(((+((()&)&)0(&&&))(+(:A(4
@HWI-H225:69:CBJ27ACXX:8:1101:12005:2537 2:N:0:AGTCAA
TCCGTTTAAGTCGGCCTTTTTTTTAATTTCCTTTTGGTACCTTCCCCCTTCCTGATTTTTTCCTCTGCTCCCG
+
J))(.=C()..)(,'3,>A(-',5((+:A((((+(()+((:4:(+9<>-8++(((+(+>C03:(4:((++:+&
@HWI-H225:69:CBJ27ACXX:8:1101:12017:2553 2:N:0:AGTCAA
TCAGTTTGAGTCGGCCTTTCTCTTAATTTCATATCGGTACCTTCCCACTTCCTGATTTTTGCCTTTGCTCCAG
+
G977)7)7?;)=8B/,355(-5;;3>;AD3((,,(,3<<8>>@9??(28@((+(+((44>&+(:(+44>44@:
@HWI-H225:69:CBJ27ACXX:8:1101:12422:2531 2:N:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGAGGGTAGTGTAGGGAAAGAGGGTAGATTTCGGTGGTCGCCGTATCATTAA
+
;CF6@'=BEFBBE;>;(;?B?8'(5(8<(<:3+(3:@(8<((315((((+((+()+(&)()0(&&)(+(44(>
@HWI-H225:69:CBJ27ACXX:8:1101:12260:2651 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAACCAG
+
?FAB<F?;FB=@(.=AB@E/9.3;;=3==953((((((5>;>AB?B<0@00<&&)+2::::ACCD9009&8+2
@HWI-H225:69:CBJ27ACXX:8:1101:12346:2651 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTGTCCTTAAAAAAAAAATAA
+
6@:FDIGGIII@A>;A@GE>D66;>A355=;?:A:@CC3,;>AB28>):9>?<0&28(:C+((4>@>55&++4
@HWI-H225:69:CBJ27ACXX:8:1101:12673:2580 2:Y:0:AGTCAA
GGATACCTAATATGTACTAAGTCCCTTGCATACATCATCACATCTAAATCTCATGCCAACCTTAATGAAGATG
+
++211*1:9CAD**1:**::FF****098*0?*?/8=F8)))))/)8)7.))7).)7).,9;@;;;;@((;(5
@HWI-H225:69:CBJ27ACXX:8:1101:12594:2610 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA
+
?CDGGGG@BFG@@D).?D@FH3@F54-:())7;B;(;>A((-(;>>@C9@(,8',&)209<8<C3+4(:AC5&
@HWI-H225:69:CBJ27ACXX:8:1101:12632:2672 2:Y:0:AGTCAA
TCTGCTTCCTTTTAACCCAAATAGGAACAGCGGCCTGTATGGGAAAAACGCAATTTTCGGTAGTTGCCGTGGT
+
F>;7)).)7).))73)).56(((-3(-(55(''))&+4:((3+9?(91))&)0&++4:&92(&+((4(&)&&(
@HWI-H225:69:CBJ27ACXX:8:1101:12828:2529 2:Y:0:AGTCAA
GGCCCCGAGCTCGGAAGAGCGGCGGGTAGGGAAAGCGTGTAGGTCTCGGTGGGGGCCGGATCATTAAAAAAGA
+
BE@>F:)60'7)'''5@@.==',8&&&)8<?(+(2(5+5&34(+++(+((2<&))&05&)&)(+++(:::&&+
@HWI-H225:69:CBJ27ACXX:8:1101:12948:2543 2:N:0:AGTCAA
GCTGCTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATTTAGGTGGGCGCCGCATC
+
<9CF9DHCEGE;)7?;=;=;C@CAC@D8=A<@8?<0889:>@(8<2(3+4++++((+(+>(4<&+)&00&)59
@HWI-H225:69:CBJ27ACXX:8:1101:12951:2594 2:N:0:AGTCAA
TCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAGAAGGAA
+
AAFHEB:FBDDGFHHI67@DGD=?==7).;?;>@>;;>A?=>;:2;?>00<?C@>:::@>505&&)&((((((
@HWI-H225:69:CBJ27ACXX:8:1101:12986:2733 2:Y:0:AGTCAA
CTGTGAAGATGGTCCAAGACTCCTCTATCATGATATTTGTACATTATTTTATTACTTCCATTTATGTTTCACT
+
***0****0*/*//**))()8)8=@))=))7))7=))=))))))))).)6;(.;(((((((-5(-(,(,(,,5
@HWI-H225:69:CBJ27ACXX:8:1101:13147:2725 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAC
+
AFHEFC?FH7CGGGGGAA:=6=@@D;@BEC@5>>ACC;>ACDD<<=3>05@>55A<4@C>CDDDB<9B50&)&
@HWI-H225:69:CBJ27ACXX:8:1101:13258:2664 2:Y:0:AGTCAA
CTTGTTGGCCCAGAGATCGGAAGAGCGTCGGGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGATTAATTAA
+
:CFA:C:B9:BB)0?9<D=0---..=@-;4'3.3;55?A92><(5(;((444(+82890>&5()&&+(4((+4
@HWI-H225:69:CBJ27ACXX:8:1101:13367:2743 2:N:0:AGTCAA
GCCGCTGGGAACAAGATCGGAAGAGCGACGTGTAGGGAAAGAGGGTAGATCTCGGTGGCCGCCGTATCATTAA
+
;CF@@/;AE>;B(.)(((.,5;',9?8'8)5?+3>A@><1((3+)++((((+(+8++5(+)&55)5A2(+4>>
@HWI-H225:69:CBJ27ACXX:8:1101:13685:2558 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGCGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAATCCT
+
DDG8BGHE:;7A(=@E4@A5?;3;CAC?AA9;(3=?8?(+:>?B(<-&45-<559AB:>C>CD>A>59.(4(+
@HWI-H225:69:CBJ27ACXX:8:1101:13743:2722 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAA
+
IGHHGGIJJEGG=FIIAGHDFECEEDDDDDDD:@ACADEDEDDDDDD9@5<?B9<ABACDCCDDDD>@90&&0
@HWI-H225:69:CBJ27ACXX:8:1101:14083:2549 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAATCTCGGTGGTCGCCGTATTATTAAAAAAAAAAATT
+
ADHGDCHEG<DA(=@@BDE''.66>A3??AC;,:5>(;B:>C<??09)()5?<00+((4>>C>:>09<0))84
@HWI-H225:69:CBJ27ACXX:8:1101:14157:2575 2:Y:0:AGTCAA
TACTCCTGAGATCGGAAGACCGAAGAGTGGGGTGAGGGGGAAGATTTCGGTGGTCGGCGGTTCCCGTAAAAAT
+
@()).)))).7);.9A1'(((3''3'8+(((0&&(((200&5??(++((0((*5@-&&0&)&+:(&+0&(:(+
@HWI-H225:69:CBJ27ACXX:8:1101:14198:2703 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAGGG
+
@FGB>FHIEBCG=FEBHIE<?;.6@CCD@B?;(::>A@CDE@BB8@B0495?>&)28ADD:>>CDB5.&&&((
@HWI-H225:69:CBJ27ACXX:8:1101:14078:2720 2:N:0:AGTCAA
GGGATTTAGTTATTCTTTATTGAAACGGAGTGTGGAGTGCTGAAGACAAAGGCAGATCGGAAGAGCGTCGTGT
+
;FB27;@??CEACE9?@D3?C3;>CCAB5;,5:>?>95<AAACC:@>CCD>?8<(8:3<@@BB<??B)295<A
@HWI-H225:69:CBJ27ACXX:8:1101:14375:2624 2:Y:0:AGTCAA
CCAGAAAGCTTCCATTCCAACAAGCTGCAGAAGAAAGTATCTGAACAACACCATTGACAGTATTTGCGTTGGT
+
..)=@@.)7).?A))=7)7@?166;3(;((5>(5((,,,(5;>A@:((+(+(1>C(4(+:4:>>C(+&0<9?(
@HWI-H225:69:CBJ27ACXX:8:1101:14655:2563 2:Y:0:AGTCAA
TCTTCCTATCTGCCTTGTTGACCCAGAGATGGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGAACTCGGTGGGC
+
))*/**0B=)=))).8@=).).)6=(.67))7?;;5A2,3',',',3:>8(((++(()(((((+(((++(0&+
@HWI-H225:69:CBJ27ACXX:8:1101:14570:2599 2:N:0:AGTCAA
TGCTCCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA
+
III?DHED3BDG?DGGHHGBFEF;@AAB=?;@D>A;A@A(;55@;@:;><?(5;&40&+)))28>@4(4>AC<
@HWI-H225:69:CBJ27ACXX:8:1101:14809:2545 2:N:0:AGTCAA
CATATTTACACATTTATTATGTTTCCCCTATATAATACAAGCTCTGAGAGGCCTAGGTTTGCTTTGCTCCCTC
+
G4C98CG@@F@@;=C4@=@E3?)77?;37;B@(>@;;(;>@=>@;(5>3@B7<3?39(4:(4:>444>:A???
@HWI-H225:69:CBJ27ACXX:8:1101:14847:2578 2:Y:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTCGGGAAAGAGGGAAGATCTCGGTTGTCGCCGGATTTTTAAAAAAAAGAGTT
+
))00**?8@;6-=F;A-@''5.(',8''(,(,(,',2?(:@:1(8&00(&(+&&&&&++(8:CC:&9&&+(,(
@HWI-H225:69:CBJ27ACXX:8:1101:14885:2609 2:N:0:AGTCAA
TCTGCCTCCTGTGAACACAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCGTATC
+
/..7=)..6)777;?@@>6;;.(('(,'(((3'(&&0((:>B38A(3(()((((++4><880)&+&&20&)28
@HWI-H225:69:CBJ27ACXX:8:1101:15164:2596 2:Y:0:AGTCAA
TGTACGGAAAGAGGTTAGAACTCGGCTGGCGCCGTATCCTTAAAAAAAAAACAGAGAACTTACGGGCGTGGCC
+
../)).815@@3)(.(.6=)7)75?(,,',,,85&)+2((4+4>:3))50&&(((+(+(++(((&)&&&&&)+
@HWI-H225:69:CBJ27ACXX:8:1101:15123:2669 2:Y:0:AGTCAA
TCGGAAGAACGTCGTGTAGGGAAAGAATGTACATCTCGCTGGACACCCTTTCATTAAAAAAAATATGAAATCT
+
D?))):)0*0?(8'7;6=@;(..B6)(7)..)7)))).((',(((,5'((,(:@,(4::+)&)(+((4>>+4:
@HWI-H225:69:CBJ27ACXX:8:1101:15470:2680 2:N:0:AGTCAA
ATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAACAA
+
GAHIG><BFD@B=C>BAHH9?;>>CCABBB?C3:@BC;>5>A<?+895:>>?&0&88>>C>ACDDB@B.0&2(
@HWI-H225:69:CBJ27ACXX:8:1101:15761:2510 2:Y:0:AGTCAA
GTCGCGGGGACCGACTTCGCGTCGGGGAGCGGAGTGGAGGGAAAGGGGGTAGATCTCGGTGCTTGCCGGACGA
+
*1*:))(',''(,',21(,&&&&)&)&0-&)0&)+((488&&+(+((&)&)2((((+<0+(&+(((3&&)&&)
@HWI-H225:69:CBJ27ACXX:8:1101:15783:2616 2:N:0:AGTCAA
TGTAGCGTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC
+
8FHGIJIHHGG1;.(=CCEC3=7@>DFFFBDDDDDDCDDDD;;8<ACD9<A<39(4(:::3:CAAB0<@&405
@HWI-H225:69:CBJ27ACXX:8:1101:16032:2528 2:N:0:AGTCAA
TGTAGCGTTGGTGTGTTCTGACTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATATCGGTGGTCGC
+
DHFD>A@6BAC(((56?;AC>7=@EDFEC=B;??555;8?B0983>>732<8>A(0((2(:(448@<@B.>&&
@HWI-H225:69:CBJ27ACXX:8:1101:16430:2627 2:Y:0:AGTCAA
CCAGAAAGCTTCCATTCCAACAAGCTGCAGAAGACAGAATCTGAAAAACACCATTGACAGTATTTGCGTTGGG
+
C;B;CH37)==AE?A>EAE)),6(;;;>(->?(-(:3(55(55@@(52&(+(+>:>@34:(+:::(()5<.(&
@HWI-H225:69:CBJ27ACXX:8:1101:16388:2655 2:N:0:AGTCAA
TCTTGCTGCTTCCTGTGAACACAGATCGGAAGAGCGTCGTGTAGGGGAAGGGGGGAGATTTCGGTGGTCGCCG
+
?8?F<??CB@)88@@=8=CE@G@D>=????DA@B@;3=@=855>@B-5><(&&9&&&03+:(+02<5&40)8)
@HWI-H225:69:CBJ27ACXX:8:1101:16394:2697 2:Y:0:AGTCAA
CCGCTGGGAACAAGATCGGAAGAGCGTCGT