##FastQC 0.11.8 >>Basic Statistics pass #Measure Value Filename Indo_SRR12416842_2_fastq_gz.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5357732 Sequences flagged as poor quality 0 Sequence length 150 %GC 66 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.91497092426422 32.0 32.0 32.0 32.0 32.0 2 30.970191118182097 32.0 32.0 32.0 32.0 32.0 3 34.05339964746277 37.0 32.0 37.0 32.0 37.0 4 35.18625119733499 37.0 37.0 37.0 32.0 37.0 5 35.36490011071849 37.0 37.0 37.0 32.0 37.0 6 38.33246623757963 41.0 37.0 41.0 32.0 41.0 7 38.475004722147354 41.0 41.0 41.0 32.0 41.0 8 38.409895082471465 41.0 41.0 41.0 32.0 41.0 9 38.59264535814781 41.0 41.0 41.0 32.0 41.0 10-14 38.74112885825569 41.0 41.0 41.0 32.0 41.0 15-19 38.58382270707083 41.0 41.0 41.0 32.0 41.0 20-24 38.487092075527485 41.0 41.0 41.0 32.0 41.0 25-29 38.35666543231353 41.0 41.0 41.0 32.0 41.0 30-34 38.272244599020624 41.0 39.4 41.0 32.0 41.0 35-39 38.21071718406221 41.0 39.4 41.0 32.0 41.0 40-44 38.18890231911563 41.0 39.4 41.0 32.0 41.0 45-49 37.809219647417976 41.0 37.0 41.0 28.0 41.0 50-54 37.70784455064195 41.0 37.0 41.0 28.0 41.0 55-59 37.64183467930087 41.0 37.0 41.0 28.0 41.0 60-64 37.4754080271279 41.0 37.0 41.0 27.0 41.0 65-69 37.439951233096394 41.0 37.0 41.0 27.0 41.0 70-74 37.14466330156118 41.0 37.0 41.0 27.0 41.0 75-79 36.98326519504896 41.0 37.0 41.0 27.0 41.0 80-84 37.16592442473792 41.0 37.0 41.0 27.0 41.0 85-89 36.94232925424415 41.0 37.0 41.0 26.0 41.0 90-94 36.74133129465976 41.0 37.0 41.0 25.0 41.0 95-99 36.49851825361925 41.0 37.0 41.0 25.0 41.0 100-104 35.99408514647616 41.0 36.0 41.0 22.0 41.0 105-109 35.55447133227268 41.0 34.0 41.0 22.0 41.0 110-114 35.0302718762342 41.0 32.0 41.0 20.0 41.0 115-119 34.27551796170469 38.6 32.0 41.0 14.0 41.0 120-124 33.6223995153173 37.0 30.0 41.0 12.0 41.0 125-129 33.0549444429098 37.0 27.0 41.0 12.0 41.0 130-134 32.31189831816896 37.0 27.0 41.0 12.0 41.0 135-139 31.533825917384444 37.0 27.0 41.0 12.0 41.0 140-144 31.029448841412744 37.0 26.0 41.0 12.0 41.0 145-149 30.509690443642945 37.0 23.0 41.0 12.0 41.0 150 30.169924139542626 32.0 22.0 41.0 12.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 9.0 12 42.0 13 168.0 14 342.0 15 871.0 16 2980.0 17 6616.0 18 11216.0 19 15912.0 20 21435.0 21 26868.0 22 33773.0 23 40171.0 24 48393.0 25 57473.0 26 67065.0 27 78307.0 28 91120.0 29 106527.0 30 124804.0 31 147004.0 32 175595.0 33 211930.0 34 260874.0 35 330174.0 36 430892.0 37 585206.0 38 823220.0 39 1091105.0 40 567639.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.79240501036515 18.659197584488222 14.285711619162672 31.26268578598395 2 35.5177837534257 22.028564277276452 17.791144697014804 24.662507272283047 3 28.579569199457893 17.973920072898217 17.861575139796553 35.58493558784734 4 34.00621755623462 16.4337447263133 16.047741843003717 33.51229587444837 5 32.34898128737471 18.128778536002265 16.979750808181358 32.542489368441665 6 31.805398254335977 16.824096464698123 17.55192308984473 33.818582191121166 7 34.44932379843523 16.600040091665825 16.472000600255107 32.47863550964384 8 31.524459349890922 17.143084322453387 18.51193738119734 32.82051894645836 9 32.13608920658616 16.35471499915729 17.835437866212146 33.67375792804441 10-14 32.99568777339646 17.190291160874306 17.128417015983388 32.68560404974584 15-19 32.95659827576321 16.896350630253103 17.012738704182414 33.13431238980127 20-24 32.76002210830276 17.126771017875605 17.146641762100117 32.966565111721515 25-29 33.05055685000767 16.986564492883037 16.974488110717765 32.98839054639153 30-34 33.06227588138065 16.752728788906353 16.90435212661101 33.28064320310199 35-39 32.89095582911961 16.90645932426723 17.017582538514414 33.185002308098746 40-44 33.11715736698517 16.88851670618858 16.92885115397956 33.06547477284669 45-49 33.08698247234404 16.815197023512198 16.75356202403052 33.34425848011324 50-54 32.97159452422333 16.883476760113204 16.91878033953853 33.22614837612494 55-59 33.18220588952802 16.879876042944176 16.7669858881147 33.17093217941311 60-64 33.1545099865008 16.70477166454472 16.746032273388995 33.39468607556548 65-69 32.99892986859673 16.84221159395003 16.894279102977013 33.26457943447622 70-74 33.19630615307176 16.75124521634892 16.79949422348328 33.25295440709604 75-79 33.13838254530457 16.687360403137205 16.74193647009838 33.43232058145984 80-84 32.98616910184834 16.83259867131457 16.843136861109645 33.33809536572745 85-89 33.152530738593796 16.852322153812203 16.754289994216844 33.24085711337715 90-94 33.141444307703495 16.71418629298721 16.713394890278547 33.43097450903075 95-99 32.943923454378165 16.844794102741524 16.853062634181654 33.35821980869866 100-104 33.19797323443244 16.785373971873838 16.675355784081543 33.341297009612184 105-109 33.2032737491634 16.64898556622693 16.60283888331 33.54490180129967 110-114 33.042060313788845 16.764035779990113 16.80662531797034 33.3872785882507 115-119 33.19389736957593 16.677735086899602 16.652705151769133 33.47566239175533 120-124 33.19470517226041 16.456521301714027 16.60346172708121 33.74531179894434 125-129 33.078019665846945 16.613050547626536 16.736449250118447 33.572480536408065 130-134 33.267874686422275 16.639592773204118 16.59908645580884 33.493446084564766 135-139 33.15621322428044 16.42213323204505 16.544851329268738 33.87680221440577 140-144 33.09754759966184 16.549690249383655 16.618440764303248 33.73432138665126 145-149 33.23874389052556 16.487453176732586 16.473491543524627 33.80031138921722 150 32.98485254581603 16.180801876614957 16.524716055226353 34.30962952234266 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 6.0 2 4.5 3 1.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.5 16 0.5 17 1.5 18 4.0 19 6.0 20 9.5 21 13.0 22 20.0 23 23.0 24 31.0 25 47.0 26 54.0 27 66.0 28 91.5 29 118.0 30 128.0 31 149.5 32 168.5 33 168.5 34 210.0 35 243.0 36 236.5 37 253.5 38 310.5 39 398.5 40 515.5 41 638.5 42 812.0 43 1044.0 44 1366.5 45 1779.0 46 2291.5 47 2946.0 48 3971.5 49 5332.5 50 7073.5 51 9527.0 52 12748.5 53 17393.0 54 24533.5 55 34712.5 56 48473.0 57 66762.0 58 90063.5 59 120614.5 60 158914.5 61 202524.0 62 250951.5 63 300811.5 64 346764.0 65 386828.0 66 414704.5 67 427162.0 68 421459.0 69 398383.5 70 361061.5 71 312173.0 72 257763.5 73 202065.0 74 151095.5 75 107791.5 76 73617.0 77 48364.5 78 30865.5 79 19049.5 80 11331.0 81 6817.0 82 4093.5 83 2421.0 84 1421.5 85 841.0 86 507.5 87 343.0 88 243.5 89 168.5 90 131.0 91 108.5 92 97.5 93 86.5 94 76.5 95 81.0 96 89.0 97 96.0 98 108.0 99 183.5 100 449.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006681931832349957 2 0.001138541457467451 3 0.002333076757105432 4 0.0 5 0.006215316480928871 6 0.0 7 1.8664614056843455E-4 8 0.0020157783181390934 9 2.05310754625278E-4 10-14 0.001534231275472532 15-19 0.0025831825854671342 20-24 0.001993380781270881 25-29 0.003120723470304226 30-34 0.004580296289549384 35-39 0.001411044822697365 40-44 5.375408848370915E-4 45-49 0.0011534731487129256 50-54 0.0037627861938596407 55-59 0.002512257052051129 60-64 0.001485703278924739 65-69 0.0013475851349040975 70-74 0.0020307100093845676 75-79 0.0013475851349040975 80-84 0.001411044822697365 85-89 0.0019112564794207699 90-94 0.0029154127156789476 95-99 0.0012990571383563044 100-104 0.0013513180577154663 105-109 6.308639551213088E-4 110-114 0.0014595728192451582 115-119 0.002747431189167357 120-124 0.003482816983006989 125-129 0.002598114276712609 130-134 9.18299011596698E-4 135-139 0.0016238214229453807 140-144 0.0018552626372502394 145-149 0.0035948046673480493 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 5357732.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.44915078293016 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2631134775968 62.163099780338285 2 14.475938337970188 22.42298262123705 3 3.3722385199683487 7.835310288271017 4 0.9721824815831066 3.0117883041861297 5 0.4033267893082943 1.5618658659966593 6 0.20903139739369037 0.9713582529066311 7 0.12517677162742438 0.6786384262204964 8 0.07540815200280894 0.46722378677821336 9 0.044564800529076826 0.3106355360208909 >10 0.058707880265943715 0.5122025432283838 >50 1.0011628673431171E-4 0.005707809631014276 >100 1.8546118594852025E-4 0.03883397031827289 >500 0.0 0.0 >1k 2.5814281642176693E-5 0.02035281486700612 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.465845622737382E-5 0.0 3.732922811368691E-5 1.8664614056843455E-5 5.5993842170530366E-5 2 1.1198768434106073E-4 0.0 9.332307028421728E-5 1.8664614056843455E-5 7.465845622737382E-5 3 1.4931691245474764E-4 0.0 1.306522983979042E-4 1.8664614056843455E-5 1.679815265115911E-4 4 1.679815265115911E-4 1.8664614056843455E-5 1.8664614056843455E-4 1.8664614056843455E-5 1.679815265115911E-4 5 2.2397536868212146E-4 1.8664614056843455E-5 2.4263998273896492E-4 1.8664614056843455E-5 2.4263998273896492E-4 6 3.1729843896633874E-4 1.8664614056843455E-5 2.986338249094953E-4 1.8664614056843455E-5 2.986338249094953E-4 7 3.359630530231822E-4 1.8664614056843455E-5 3.359630530231822E-4 1.8664614056843455E-5 3.359630530231822E-4 8 3.732922811368691E-4 1.8664614056843455E-5 3.732922811368691E-4 1.8664614056843455E-5 4.2928612330739947E-4 9 4.2928612330739947E-4 7.465845622737382E-5 4.666153514210864E-4 1.8664614056843455E-5 4.8527996547792984E-4 10-11 5.039445795347733E-4 7.465845622737382E-5 5.412738076484601E-4 1.8664614056843455E-5 5.786030357621472E-4 12-13 6.905907201032079E-4 7.465845622737382E-5 6.53261491989521E-4 1.8664614056843455E-5 6.719261060463644E-4 14-15 8.772368606716425E-4 7.465845622737382E-5 9.238983958137511E-4 1.8664614056843455E-5 7.372522552453165E-4 16-17 0.0011945352996379811 1.02655377312639E-4 0.001063883001240077 1.8664614056843455E-5 8.025784044442686E-4 18-19 0.0013625168261495722 1.1198768434106073E-4 0.0011105445363821856 1.8664614056843455E-5 8.305753255295337E-4 20-21 0.002034442932195937 1.306522983979042E-4 0.001231864527751668 1.8664614056843455E-5 8.865691677000642E-4 22-23 0.002874350564753892 1.8664614056843455E-4 0.0013811814402064156 1.8664614056843455E-5 9.705599309558597E-4 24-25 0.003732922811368691 2.05310754625278E-4 0.0015491629667180068 1.8664614056843455E-5 0.0010545506942116552 26-27 0.0043768519963297906 2.613045967958084E-4 0.0017078121862011762 2.7996921085265183E-5 0.0011478737644958726 28-29 0.004722147356381394 2.7996921085265183E-4 0.0017731383354001283 4.666153514210864E-5 0.0012038676066664028 30-31 0.004918125803978251 3.1729843896633874E-4 0.0018757937127127672 5.5993842170530366E-5 0.001269193755865355 32-33 0.005114104251575107 3.732922811368691E-4 0.0020437752392243585 6.53261491989521E-5 0.0013158552910074637 34-35 0.005319415006200385 4.10621509250556E-4 0.002127766002480154 8.399076325579555E-5 0.001446507589405368 36-37 0.00561804883110988 4.479507373642429E-4 0.0023424090641338538 9.332307028421728E-5 0.0014931691245474764 38-39 0.005720704208422519 4.666153514210864E-4 0.002529055204702288 9.332307028421728E-5 0.0015771598878032722 40-41 0.005926014963047797 5.13276886563195E-4 0.0026970367312138793 1.4931691245474764E-4 0.0016518183440306458 42-43 0.006140658024701497 5.692707287337255E-4 0.00280902441555494 1.679815265115911E-4 0.0017451414143148632 44-45 0.0062526457090425576 6.065999568474123E-4 0.0030703290123507486 1.679815265115911E-4 0.0018851260197411891 46-47 0.006476621077724679 6.252645709042558E-4 0.0032196459248054962 2.05310754625278E-4 0.0019411198619117193 48-49 0.006784587209662596 6.53261491989521E-4 0.0033129689950897132 2.1464306165369974E-4 0.0020811044673380453 50-51 0.007055224113486826 6.625937990179427E-4 0.0034342889864591958 2.2397536868212146E-4 0.0021744275376222627 52-53 0.00728853178919737 6.719261060463644E-4 0.0035182797497149915 2.4263998273896492E-4 0.002258418300878058 54-55 0.007531171771936334 6.719261060463644E-4 0.0036395997410844736 2.613045967958084E-4 0.0023144121430485887 56-57 0.0077924763687321425 7.092553341600513E-4 0.0037702520394823783 2.706369038242301E-4 0.002426399827389649 58-59 0.008035116351471108 7.465845622737382E-4 0.0039195689519371255 2.986338249094953E-4 0.002613045967958084 60-61 0.008324417869352181 7.652491763305817E-4 0.004096882785477138 3.1729843896633874E-4 0.002734365959327566 62-63 0.008511064009920615 7.839137903874251E-4 0.004274196619017151 3.639599741084474E-4 0.002846353643668627 64-65 0.008847027062943798 8.305753255295337E-4 0.004423513531471899 3.732922811368691E-4 0.002893015178810736 66-67 0.009136328580824872 8.399076325579555E-4 0.004507504294727694 3.826245881652908E-4 0.0029583413280096877 68-69 0.009481623940876475 8.399076325579555E-4 0.004638156593125599 3.9195689519371256E-4 0.003014335170180218 70-71 0.009789590072814392 8.399076325579555E-4 0.004731479663409816 3.9195689519371256E-4 0.0030703290123507486 72-73 0.010116220818809153 8.399076325579555E-4 0.004936790418035094 4.2928612330739947E-4 0.0031636520826349656 74-75 0.010405522336690226 8.585722466147989E-4 0.0050861073304898415 4.479507373642429E-4 0.0032756397669760264 76-77 0.010722820775656564 8.772368606716425E-4 0.005216759628887745 4.8527996547792984E-4 0.0033409659161749783 78-79 0.011030786907594483 8.772368606716425E-4 0.005422070383513024 5.039445795347733E-4 0.003434288986459196 80-81 0.011422743802788194 8.772368606716425E-4 0.005571387295967772 5.13276886563195E-4 0.003527612056743413 82-83 0.011861362233124017 8.865691677000642E-4 0.0057020395943656755 5.599384217053037E-4 0.0036489320481128953 84-85 0.012243986821289306 9.052337817569076E-4 0.0057953626646498925 5.786030357621472E-4 0.0037235905043402693 86-87 0.01264527602351144 9.332307028421728E-4 0.005982008805218327 5.972676498189906E-4 0.003844910495709752 88-89 0.013102559067904106 9.61227623927438E-4 0.006084664182530966 5.972676498189906E-4 0.0038915720308518604 90-91 0.013830479016121 9.798922379842814E-4 0.006177987252815184 6.159322638758341E-4 0.003975562794107656 92-93 0.014521069736224208 9.89224545012703E-4 0.006289974937156244 6.159322638758341E-4 0.004124879706562404 94-95 0.015472965053123225 9.89224545012703E-4 0.00637396570041204 6.159322638758341E-4 0.0042275350838750425 96-97 0.01626621115053907 9.89224545012703E-4 0.006485953384753101 6.252645709042558E-4 0.004302193540102417 98-99 0.017274100309608617 9.98556852041125E-4 0.006607273376122583 6.345968779326775E-4 0.004404848917415056 100-101 0.01851529714438871 0.0010078891590695467 0.006747257981548909 6.345968779326775E-4 0.004479507373642429 102-103 0.01961650937374247 0.0010078891590695467 0.006887242586975235 6.345968779326775E-4 0.004600827365011911 104-105 0.02068039237498255 0.0010078891590695467 0.007111217955657357 6.345968779326775E-4 0.004731479663409816 106-107 0.022173561499530026 0.0010078891590695467 0.007157879490799465 6.345968779326775E-4 0.004740811970438238 108-109 0.02381604753653225 0.0010078891590695467 0.007307196403254213 6.439291849610993E-4 0.004815470426665611 110-111 0.025570521257875532 0.0010452183871832335 0.007363190245424743 6.53261491989521E-4 0.00486213196180772 112-113 0.027716951874412532 0.0010452183871832335 0.0074845102367942255 6.53261491989521E-4 0.004946122725063516 114-115 0.029732730192551626 0.0010825476152969205 0.007540504078964756 6.625937990179427E-4 0.004983451953177203 116-117 0.03183249927394651 0.0010918799223253422 0.007605830228163708 6.719261060463644E-4 0.005104771944546685 118-119 0.03452953600516039 0.001129209150439029 0.007773811754675299 6.719261060463644E-4 0.005216759628887745 120-121 0.037758514236994306 0.0012038676066664028 0.007895131746044781 6.719261060463644E-4 0.005356744234314072 122-123 0.04161275703973248 0.0012505291418085116 0.007960457895243734 6.719261060463644E-4 0.005431402690541445 124-125 0.04518703063161801 0.0012878583699221984 0.008137771728783747 6.905907201032079E-4 0.0055153934537972415 126-127 0.04983451953177202 0.0012971906769506203 0.00821243018501112 6.905907201032079E-4 0.005543390374882507 128-129 0.05640446367978092 0.001334519905064307 0.008464402474778508 6.905907201032079E-4 0.005590051910024615 130-131 0.08014585276008579 0.0013811814402064158 0.008557725545062723 6.905907201032079E-4 0.005636713445166724 132-133 0.08746238147036844 0.0013811814402064158 0.008679045536432207 6.905907201032079E-4 0.005739368822479362 134-135 0.09434029175031525 0.0013998460542632592 0.008735039378602738 6.905907201032079E-4 0.005851356506820424 136-137 0.1020487773557916 0.0014278429753485245 0.008893688598085907 6.905907201032079E-4 0.0059166826560193755 138-139 0.1107744844273659 0.0014651722034622113 0.009126996273796449 7.185876411884731E-4 0.006047334954417279 >>END_MODULE