slurmstepd: error: couldn't chdir to `/srv/pulsar/main/venv/lib/python3.6/site-packages': No such file or directory: going to /tmp instead [2020-09-14 19:52:52] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2020-09-14 19:52:52] Checking for Bowtie Bowtie version: 2.2.8.0 [2020-09-14 19:52:53] Checking for Bowtie index files (genome).. [2020-09-14 19:52:55] Checking for reference FASTA file [2020-09-14 19:52:55] Generating SAM header for /cvmfs/data.galaxyproject.org/byhand/hg19/hg19full/bowtie2_index/hg19full [2020-09-14 19:53:58] Preparing reads left reads: min. length=76, max. length=76, 156798 kept reads (0 discarded) [2020-09-14 19:54:12] Mapping left_kept_reads to genome hg19full with Bowtie2 [2020-09-14 19:54:40] Mapping left_kept_reads_seg1 to genome hg19full with Bowtie2 (1/3) [2020-09-14 19:54:50] Mapping left_kept_reads_seg2 to genome hg19full with Bowtie2 (2/3) [2020-09-14 19:54:57] Mapping left_kept_reads_seg3 to genome hg19full with Bowtie2 (3/3) [2020-09-14 19:55:04] Searching for junctions via segment mapping [2020-09-14 19:57:35] Retrieving sequences for splices [2020-09-14 19:58:55] Indexing splices Building a SMALL index [2020-09-14 19:59:03] Mapping left_kept_reads_seg1 to genome segment_juncs with Bowtie2 (1/3) [2020-09-14 19:59:06] Mapping left_kept_reads_seg2 to genome segment_juncs with Bowtie2 (2/3) [2020-09-14 19:59:10] Mapping left_kept_reads_seg3 to genome segment_juncs with Bowtie2 (3/3) [2020-09-14 19:59:14] Joining segment hits [2020-09-14 20:00:42] Reporting output tracks ----------------------------------------------- [2020-09-14 20:02:35] A summary of the alignment counts can be found in ./tophat_out/align_summary.txt [2020-09-14 20:02:35] Run complete: 00:09:43 elapsed [E::idx_find_and_load] Could not retrieve index file for '/jetstream/scratch0/main/jobs/30603555/working/tophat_out/accepted_hits.bam' [E::idx_find_and_load] Could not retrieve index file for '/jetstream/scratch0/main/jobs/30603555/working/tophat_out/accepted_hits.bam'