@HWI-H225:69:CBJ27ACXX:8:1101:1730:1976 1:Y:0:GGCCTG
CCCCTCGCGTTGTTCAGATACTGTCTTCTGCACCTTGTTGGAATGGAAGCTTTCGGAAACAAAAAATCGCTGT
+
):9(7((('-'--66:)):);33.918==)9=(.(6((9(,-9:0((,;<:0(7-0888(628<()(+(((&)
@HWI-H225:69:CBJ27ACXX:8:1101:2404:1969 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
;1@<<<?????2=7?>(<<?6=>59=1:)6>)86;?5=?8=(6-(-3(,(8;,7;;'+(+(86::2+45:;9:
@HWI-H225:69:CBJ27ACXX:8:1101:3220:1936 1:Y:0:GGCCTG
CCACCNNGNGAANNGTCNTCTCNNCNNNGGCTGNNATGNNNNCTNNACNNANNANGAACANGANNANNAANAT
+
?1>>?##1#0:9##00<#0<;;##-###--:?=##,(,####,,##,,##,##+#++668#++##+##+2#+2
@HWI-H225:69:CBJ27ACXX:8:1101:3921:1933 1:Y:0:GGCCTG
TCACANNGNAAGNNCACNCGTNNNNNNNCCAGTNNNCGNNNNNTNNCANNNNNCNTCTTCNCCNNTNNAANTG
+
(7((.##-#--(##-((#--,#######,,,(,###,(#####+##+(#####+#(++2(#((##+##++#((
@HWI-H225:69:CBJ27ACXX:8:1101:3869:1935 1:Y:0:GGCCTG
AAGCCNNTNTGTNNGTANAAAANNANNNGATGTNNACTNNNNNCNNATNNGNNGNGCACANGTNNGNNCTNCA
+
><89>##1#0:=##00<#0<??##-###--;=?##,8=#####,##,,##,##,#++288#++##(##++#+2
@HWI-H225:69:CBJ27ACXX:8:1101:3872:2000 1:N:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATG
+
G@BGFIIBFGFABF@F8BDF@BFB<BFEFGFGEFFIIEEFFFDDDBD@>BA;ABBAB3>@@?@B<BB?B@<8>
@HWI-H225:69:CBJ27ACXX:8:1101:4166:1997 1:N:0:GGCCTG
GACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTT
+
EFIFIIEFFFFFFAFIIIIIDA===EEBBDD@CACCCBBABBBBB=BBB<<>ADB@?@A?@A@59BBBA(::>
@HWI-H225:69:CBJ27ACXX:8:1101:5003:1927 1:Y:0:GGCCTG
TCTCNNNNNNACNNNNCNGNANNNNNNNAACNNNNNCNNNNNNTNNNNNNNNNNNNTCTTNNNNNNNNNANAA
+
22))######00####-#(#-#######--(#####(######(############++((#########+#+(
@HWI-H225:69:CBJ27ACXX:8:1101:5036:1996 1:N:0:GGCCTG
CTCTTCCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATG
+
)?GEGFFDACFFIEDEFIE==)7;BD?B@9(;;(;;@B?B:5<?<:A3>:A>>4@@@0<BBBB<4>BBB<8A3
@HWI-H225:69:CBJ27ACXX:8:1101:5483:1957 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGCATTCCGCCGTCCTGTTTCACAATA
+
)2.<>16;=950=11:(<9(..)-.633=;.=.)).'5'5',668;1;(,8':6(',&05&+(+++((++(((
@HWI-H225:69:CBJ27ACXX:8:1101:5667:1933 1:Y:0:GGCCTG
TCAGANNGNAAGNNCACNCGTNNNNNNNCCAGTNNNGGNNNNNTNNCGNNTNNCNTCTTCNGCNNGNNAANAA
+
@@???##0#0:=##007#---#######--:==###,,#####,##,,##,##+#++285#(+##+##++#+2
@HWI-H225:69:CBJ27ACXX:8:1101:8044:1921 1:Y:0:GGCCTG
TCAGNNNNNNNNNNNNCNNNNNNNNNNNCCANNNNNNNNNNNNNNNNNNNNNNNNNCTTNNNNNNNNNNNNNA
+
(0<6############(###########,,(#########################(++#############(
@HWI-H225:69:CBJ27ACXX:8:1101:8739:1922 1:Y:0:GGCCTG
CCACNNNNNNNNNNNNCNTNTNNNNNNNGGCNNNNNTNNNNNNNNNNNNNNNNNNNAACANNNNNNNNNANNT
+
=???############.#-#-#######-,9#####,###################++8:#########+##(
@HWI-H225:69:CBJ27ACXX:8:1101:8684:1990 1:Y:0:GGCCTG
ACTAGAGTCATATGGGGAGAGCACACCTCTGATCTCCAGACACCGCCCGCTCTCACAAAGCCACCTCCCATAC
+
3=))2))1):).)):((0=/(9<>)3-;3;:1)<):;1)),(3(39'''',7(3+(+2(((((++2((+((((
@HWI-H225:69:CBJ27ACXX:8:1101:8923:1968 1:N:0:GGCCTG
TATGTGACTTTAGCATGTTTAATAATGATGACAATAAAAAAAGCCCTCAAATGGGGGCATTGAACCTCTAAAA
+
@FGCGHIDGDGHEIGGI?BGFH4?F@=CBAFHHGIIIIHGHE:AACCCBCCCAC?BBBBCCCCCCBCCCCCCC
@HWI-H225:69:CBJ27ACXX:8:1101:9168:1925 1:Y:0:GGCCTG
TCGGNNNNNNACNNNNCNGNANNNNNNNCACNNNNNGNNNNNNTNNNNNNNNNNNNGCTTNNNNNNNNNANNA
+
????######00####-#.#-#######,,6#####(######(############((+2#########+##+
@HWI-H225:69:CBJ27ACXX:8:1101:9204:1967 1:Y:0:GGCCTG
GACTCGAGTCAGATCGGAAGAGCACACGGCTGAACTCCAGTCACGGCCTGATCTCCAATGCCGTCGTCTGCTT
+
;??DDBA?9909B<?8@@CDD()8@CDA':,4(;>;@AAA>;-53'8;?<9>A<A((++(+40&5&&(((++4
@HWI-H225:69:CBJ27ACXX:8:1101:9318:1933 1:Y:0:GGCCTG
GCTCTNNCNATCNNACTNGAGNNNNNNNGGAAGANNACNNNNNTNNACNNCNNTNACGGCNTGNNCNNGTNTG
+
<????##0#-7=##--6#-8=#######,,,3;;##,,#####,##++##+##+#++66:#+2##(##(+#++
@HWI-H225:69:CBJ27ACXX:8:1101:9958:1996 1:Y:0:GGCCTG
TTATTAATACATCCCAAACATAGCATCATAGAGTAGTATGGCAGCCTCACCACACAAAAAAAAAATCGATGCC
+
1))))))..):)..:>)=<?>??1)9)..=>81)>>?>8>9(;=(()89(6==<<8;765;:605((+(2/((
@HWI-H225:69:CBJ27ACXX:8:1101:10115:1968 1:Y:0:GGCCTG
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGCATGCCCCCCTCTTCCTGCCAAAAA
+
(56==>?(<63=1():.=>3(69;9>639?;0=088//;;'5,,,2=(()+(85+25)&&+(+((((((((+(
@HWI-H225:69:CBJ27ACXX:8:1101:10735:1936 1:Y:0:GGCCTG
TATCGANTNGAGNNAGAACGGGNNANNNTACAACNGAANNNNAGNNACGNCNNGNCCTCCNATNNCCNCCNCT
+
6(-.71#(#(((##,(,(79/'##(###(,,(((#+((####((##++(#+##(#+(+2(#((##+(#+(#((
@HWI-H225:69:CBJ27ACXX:8:1101:11163:1924 1:Y:0:GGCCTG
TCGGNNNNNNACNNNNCNGNANNNNNNNCACNNNNNGNNNNNNGNNNNNNNNNNNNGCGTNNNNNNNNNANNG
+
;1==######-(####-#-#-#######,,5#####,######(############+((+#########(##(
@HWI-H225:69:CBJ27ACXX:8:1101:11095:1941 1:N:0:GGCCTG
GCTCTTNCGATGTNGACTCGAGTCAGNNCGGAAGNGCANNNGTCTGAACTCCNGTCACGGNCTGNTCTCGNAT
+
D:DFII#-<CDCF#-;CC=EFDDDDD##,,;=@B#,5<###,+28<ABAABB#+2<BBBB#+2<#(+2<?#+2
@HWI-H225:69:CBJ27ACXX:8:1101:11189:1956 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGTCAACAAAAAAAAAATC
+
IGGIIIIIIIIIIIIIIIIIIIGIIIGIIIIIIIEIIIIIH@HHFEFFFCCCCBBCCCDCCCBCBBCCCBC?3
@HWI-H225:69:CBJ27ACXX:8:1101:11135:1968 1:N:0:GGCCTG
GTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGGTCTCGTATGCCGTCTTCTT
+
D2B@2@;AD@A=C=CDCD???@8>;>A93<?5=<?>5>AAA4>>>:>;?9>00((((+9+2(3(+)&)(((((
@HWI-H225:69:CBJ27ACXX:8:1101:11506:1993 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCCTTTTCTTCAAACAAAACACA
+
@FGFCHHFA>BC;B??FGHG9FCCG@F=F>D8A<D<7;.6(;((,(,3>',,',(((+(((+:C((((((((2
@HWI-H225:69:CBJ27ACXX:8:1101:12184:1928 1:Y:0:GGCCTG
GATGNNNANNCGNNNNANANCNNNNNNNCACNCNNNTNNNNNNCNNNNNNNNNNNNATCTNNNNNNNNNTNTT
+
??>?###0##00####-#-#-#######--9#,###,######,############++28#########+#++
@HWI-H225:69:CBJ27ACXX:8:1101:12189:1961 1:Y:0:GGCCTG
CGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATTTCGTATGTCGTCT
+
?<<=????<(<86>==;><???55(8577?)6=7==;=9;7;=<;;<=<:=5;7:-6/+(28(+(36+52266
@HWI-H225:69:CBJ27ACXX:8:1101:12040:1963 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATTTCGGATGCCGTCTTCTCCTTTTAAAAAT
+
A=4=4=>AAAA09>BA>=><=6)==BBBBAA;=A9>',3=',;;((,,',,'(50&&+2+((++4(+3(38+(
@HWI-H225:69:CBJ27ACXX:8:1101:12294:1928 1:Y:0:GGCCTG
CCACNNNGNNAANNNNCNTNTNNNNNNNGGCNGNNNTNNNNNNTNNNNNNNNNNNNAACANNNNNNNNNANCG
+
@??@###1##08####0#0#-#######--=#-###,######,############++8<#########+#++
@HWI-H225:69:CBJ27ACXX:8:1101:12380:1972 1:Y:0:GGCCTG
CTCGAGTCGGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTCCTCTCGTATGCCCCCTTCTCCCTGC
+
=(=((;)<':5(6=859;(97(,27',77+38<:(32+26/35-8:&(((+675(++((8(()&+2((3(+((
@HWI-H225:69:CBJ27ACXX:8:1101:12720:1971 1:Y:0:GGCCTG
CTCGAGTCAGATCGGAAGCGCACACCCCTGAACTCCAGCCCCGTCCCGATCTTGTTTACCGTCTTTTCCCTTA
+
<;(;;(:=>8>?)091-(('''''.(-'((..).;873(5(,3',8/'&&)8(22+6(+()(0(22:42(8:;
@HWI-H225:69:CBJ27ACXX:8:1101:13142:1977 1:Y:0:GGCCTG
CGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGACGTCCTTTCCTTTGAAGAAGTGAAGG
+
?AA)54?:A7=;?4==;7*/9?>=)9>=)85<<<<<;).)7(((.((-(('(5(,,,,5(((((((((+++((
@HWI-H225:69:CBJ27ACXX:8:1101:13451:1972 1:Y:0:GGCCTG
GCTCTTCCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCACGTAT
+
5=?<??=?44==:>;/5=/6:,6=??;3.65,9/(,5;;<7,,82+/23:56:<:2:<5&&)&2582(+(+8/
@HWI-H225:69:CBJ27ACXX:8:1101:13592:1939 1:N:0:GGCCTG
TCAGATNGGAAGNNCACACGTCNGANNNCCAGTCNCGGNNNGATNNCGTATNNCGTCTTCNGCNNTAAAANAA
+
GHJJAD#0?DG@##077BAAFG#-;###--;5;B#,5=###,,,##,55?7##+++<?@A#+2##(++8?#++
@HWI-H225:69:CBJ27ACXX:8:1101:13680:1948 1:Y:0:GGCCTG
ATTTCGACTAAAGGCACATGGGAAAAGNACACGTATGACCTCCAGTCCCGGCNCGAATTCGTATTTCTGAATT
+
1:)())(-(5)))).6(.).37((,9(#(,88(,5((((+:(2+(4(+:&&)#(+(2-8:(+(+((5(+((+(
@HWI-H225:69:CBJ27ACXX:8:1101:13624:1997 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGCATTCCGTCTTCTGCTTTTAAAAAA
+
GHGJGCIGBGFEHHDHHEGBFEAFDHI@DHCCHD?DFFDB;;=;3>;9(,5',;542(2::(4+>(((+4+3&
@HWI-H225:69:CBJ27ACXX:8:1101:14292:1937 1:Y:0:GGCCTG
GTAGAANTNGATNNCACAGAAANAANNNAAGAGTNGAANNNNAGNNCCACCNNANAAAAANAANNACNATNTC
+
(<3=)<#(#--8##00(((.)1#--###(--((.#,(,####,,##,,,7,##+#++886#+2##(+#++#+(
@HWI-H225:69:CBJ27ACXX:8:1101:14387:1970 1:Y:0:GGCCTG
AAACCCTGGGAACGGTAATCTGTACTGTAGCTCACATGGCATATGGACACACCAAGCAGAAGAAAAAAAAAAC
+
6('-((833(66(''(((..(..(...(((-4(:(;((((((,5;0:;(((&((+(+(((6(+(+(+5::58&
@HWI-H225:69:CBJ27ACXX:8:1101:14612:1941 1:Y:0:GGCCTG
ACAGATNGGAAAANCACACGGCTGAANNCCAGTCNCGGNNNGCTCTCGAATGNCGGCTTCNTCTNTTAAANAA
+
(<;<76#-7=((.#(--:/5'395;<##(,5((7#,((###+++88<&(+68#++((25(#((+#((+++#((
@HWI-H225:69:CBJ27ACXX:8:1101:15205:1954 1:Y:0:GGCCTG
TGTATCGATGTCGACTCGAGTCAGATCGGAAAAGCAAACGTCTGAAATCCAGNAAACGCCTAATCTCGACGTC
+
AA=:=?AB=AA4=AA<4A.=)8.)>.).-;A'7)5((6(,,;5(,5(55;35#(+((()5(&++(++((&&)&
@HWI-H225:69:CBJ27ACXX:8:1101:15803:1961 1:Y:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAAATCGA
+
BCBAAAABBABBBBBABBBBBBBBBBBBBBBBA@BBBBBBAAAAAA@@@A@@A@?>?A??A=A======88(2
@HWI-H225:69:CBJ27ACXX:8:1101:15993:1962 1:Y:0:GGCCTG
ACTCTTCCGATGTCGACTCGGGTCAGATCGGAAGAGCACACGACTGAACTCGGCCTGATCTCGCATGCCCTCT
+
:=<?<??8(7=6:>??5??/(5:=7;;839=<<;<((:76(,'0506:(8<2285&(((+3:(()++22((+2
@HWI-H225:69:CBJ27ACXX:8:1101:15776:1981 1:Y:0:GGCCTG
TGTCAACAAAGGAAAAATAGAACGCAAAAAAACATGAACTTCCATCCCGGCCTGATCCCGTTTGCCCTCTTAT
+
0):9)7<1(()07)<.(.))/.)-5''-.9''',((,72;(:((,,(28&0&&++28((+(((+(5(46((((
@HWI-H225:69:CBJ27ACXX:8:1101:15779:2000 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCTATTCCGCCTTTCTCTTTTAAAAAA
+
?EEFF4C?@DB0B))9(9(-8=CFCA@>FF===.?E8'9<',;3>:>(;3((555'(,,((((++(+4>AAA&
@HWI-H225:69:CBJ27ACXX:8:1101:16174:1979 1:N:0:GGCCTG
GCTCTTCCGATCTGCCTTTGTCTTCAGCACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG
+
6FFGGGGIHGJIJEIJGGIJHGFEHHHIGHFFEFFEFFEDEECDBDDDDDDDDDBABDBCDCCDDC@>CDDDB
@HWI-H225:69:CBJ27ACXX:8:1101:16417:1978 1:Y:0:GGCCTG
AATGTCGACTCGAGTCAGATCGGAAGAGAACACGACTGAACTCCAGTCACGGCCTGATCTCGCATTCCCCCTT
+
);>811(=??433;.09>3)<7=535=1).66)'',9=79((.-;(--:1;<;<<(+:;/:/()+&(+(+)5/
@HWI-H225:69:CBJ27ACXX:8:1101:16787:1930 1:Y:0:GGCCTG
TCGATNNCNNCTNNAGTNAGANNNNNNNAGCACNNNTCNNNNNTNNNNNNNNNNNNCCACNCCNNANNNCNGC
+
<???=##-##(-##(,(#,(,#######(,(,2###(+#####+############(+((#(+##(###+#((
@HWI-H225:69:CBJ27ACXX:8:1101:16832:1944 1:Y:0:GGCCTG
GACAGCCAGTGCAGCTTCTGAGTTGANNCTGAGCAAGGNACAGCTCTATGAGNTGTTTCAGAACATTCTTGGG
+
IIEECDDIDEEEEEEECDEIIDEAAD##-5?C@DDDA@#,;>??AAAAA3>>#,8<??AEAAA?A>AADEA>>
@HWI-H225:69:CBJ27ACXX:8:1101:16904:1980 1:N:0:GGCCTG
TATGTGACTTTAGCATGTTTAATAATGATGACAATAAAAAAAGCCCTCAAATGGGGGCATTGAACCTCTAAAA
+
FBD?:BDD@B9DB<FBD/?BBE=BC4=BF4=CFEE==@@@?B9(,55;?ABB5(9(&)59:AAAB<<?:::AA
@HWI-H225:69:CBJ27ACXX:8:1101:17153:1978 1:Y:0:GGCCTG
CTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGGATGCCGTCTTCTGCTTGA
+
)29=??;93<8?9??????(:;.;6-..6=?@;9)8;;366==/,33;(:(;6;(82&(8:)&((+((++3((
@HWI-H225:69:CBJ27ACXX:8:1101:17405:1989 1:Y:0:GGCCTG
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTCCCGTTTCTGCTTTTAAAAAA
+
(;=99>>?????;?9?>?????;;>?;=;?>59;;=<<<<<<77===;(+3+(+(&(+(++((+3(((((+(&
@HWI-H225:69:CBJ27ACXX:8:1101:18664:1984 1:Y:0:GGCCTG
TACCGGAGCCAGATCGTAAGAGCACACGGCTGAAATCCAGTCACGGGCTGAAATCACAGGTCACCGTTCTGCG
+
).8)(:0((7)6(<;;)(8=)8))()(5'',',-(-(-((,,,(35'08972(+(((((++8((52(++(((&
@HWI-H225:69:CBJ27ACXX:8:1101:18528:1988 1:Y:0:GGCCTG
GTGGTAGGTATTCTTGTCGCATAAAACCAAAATAGAAATGTCTGCGCTGAAAATATAGAGATCTGCACTCTCA
+
)***0**)**00=***9*(0'(-=A==((.(.7))..;;)..7);',3,',((((,5,(5((((((((+3=:3
@HWI-H225:69:CBJ27ACXX:8:1101:18511:1996 1:N:0:GGCCTG
ATATTATCTCTTCTTGTCCCGTAAAACCGAAATACAAATGTCTGCACTCAAAATATAGATGTCTGCACTCTCA
+
4BDAGICF<BBF90B*BD>FB(7AFFFFEB6?=)7?BC>ABBABBB>(;?BBA@ADA@B:5(,;:AABB9@AB
@HWI-H225:69:CBJ27ACXX:8:1101:18981:1924 1:Y:0:GGCCTG
TCAGNNNNNNAGNNNNCNCNCNNNNNNNCCANNNNNGNNNNNNANNNNNNNNNNNNCCTCNNNNNNNNNANNA
+
=<)=######00####-#-#-#######,,,#####,######(############+((+#########+##+
@HWI-H225:69:CBJ27ACXX:8:1101:19407:1947 1:Y:0:GGCCTG
GAATATAGAGGAACTGGCTAGACACCTNCAGGAGGCCGGCCATGAAGTCGAANCTGGCTCACTCCTCCTGGCT
+
ABBBBBBBBBBBBBBBBBBBABBBBAB#-=A@@@@@@==AA=?A?????===#(+28;=?????=?=?<??==
@HWI-H225:69:CBJ27ACXX:8:1101:19390:1953 1:Y:0:GGCCTG
GAATATAGAGGAACTGGCTAGACACCTGCAGGAGGCCGGCCATGAAGTCGAANATGGCTCACTACTCCCGGCT
+
<<?9=3>?=??>>3).9>>>71==?<?>(733553,9;<2;60;;::8;<8<#(+28(86<:6+3228(0650
@HWI-H225:69:CBJ27ACXX:8:1101:19288:1974 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAAATCGA
+
FHGCHIEGGGIGIBF?FGCHCHGCC>FFAGIEGGHEAC?CEEFFDCEECDB?BDB<?CDDDDABDDBDD<><A
@HWI-H225:69:CBJ27ACXX:8:1101:19528:1999 1:Y:0:GGCCTG
GTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATCTCCTCTTCCCCCTTA
+
)11CDA08B?DAD;-(54(63A(=64(6)(7?@.;(;;(;>>''','5,+:4+2(23+(+((+4+44((&&((
@HWI-H225:69:CBJ27ACXX:8:1101:20223:1976 1:Y:0:GGCCTG
AAGCAAATCTGTCTGTAAAAAAAAAATCGATGTTGACTCGAGTCAGATCGGAAGAGAACACGTCTGAAATCCA
+
6<31)@>8<8<>?):):>;?????<?76(378938==;(,9'-,5::;,8/896852668+&(+26:<((86:
@HWI-H225:69:CBJ27ACXX:8:1101:20114:1978 1:Y:0:GGCCTG
GTAAAGACCCATAACTTCAATAGCTCTGGAGAGTTGGCTGGTTTACCAGTACCAGGCATAATTTCCCTCCTCT
+
.@>??>?<?>:?7<31=1>><?1=>?=;><7>?=))):;?89?@@89.)69;==(,;<<>;=>=;=08;<7(,
@HWI-H225:69:CBJ27ACXX:8:1101:20172:1993 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCCGAACTCCAGTCACGGCCTGATACCGTATGCCCTCTTCCTCCTGCAAAAAA
+
:)0)7.:((0==?(6>?365<6(-553(6.=7;>;=5'322(,((+5&((+5::((+8((((+((((+65(+&
@HWI-H225:69:CBJ27ACXX:8:1101:20436:1993 1:Y:0:GGCCTG
AATAATTACACTAATCGCCACTTCTATAACAGCTATGTACAGCATACGAATCATTTACTTCGTAACAATAACA
+
9)1:8).303)0:=3<;95=5>?>>)3))..3(:6::6989=6=;8.6996,9357:1((,8;<;32:76:((
@HWI-H225:69:CBJ27ACXX:8:1101:20853:1935 1:Y:0:GGCCTG
TCAGCANTNGATNNCACNCATANNANNNTAGAGTNGTANNNNAGNNTCNNANNANAAAAANAANNTCNATNTT
+
1).9).#0#(00##0--#(---##-###-(-,5.#,,8####,,##(,##(##+#++25-#+2##(+#(+#+(
@HWI-H225:69:CBJ27ACXX:8:1101:20925:1983 1:Y:0:GGCCTG
ACTCGCGGGAGATCGGCAGAGCACACCCCCGCACTCCAGTCACGGCCCGATGTCCGCTTCTCGCCTCTTTTTC
+
00(-((-''5''(((93'3((:288((&0&&&&&&+26(+522)&&)&5-8&+42(5&))++((&&&((++8(
@HWI-H225:69:CBJ27ACXX:8:1101:20846:1996 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAAATCGA
+
>F@C?@BFFF>BDGF9BFE*?@?9BFFIIC==FADCEEIEEEEECDDB?=@BC(=????BB@B?B999@8+2<
@HWI-H225:69:CBJ27ACXX:8:1101:1418:2014 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAGAAAAAAAAATC
+
FAEEE<;BDD8BD@D?BBDCCEE9CDCCB@A;@>AEC;=AADD>7?>DDD@@@??????AA??=?????>?<:
@HWI-H225:69:CBJ27ACXX:8:1101:1739:2096 1:Y:0:GGCCTG
CTCGAGTCAGAACGGAAGACCACACGACTCAACTCCAGTCACGGCCTGATCTCCCATCCCGTCTTCTGCTTGA
+
3AB;;=?A<AA*))87<8A7).9=(''--'.77>@9>@;>5><,3;38(,,(5,,88((+((+28(3(+++(+
@HWI-H225:69:CBJ27ACXX:8:1101:1603:2193 1:Y:0:GGCCTG
TCAGAACGGAAGAGCATACGTCTGAACTACAGTCACGTCCTGATTTCGTTTATCGAATTCTGGTATCAAAGAA
+
116)9.)((7-(97)9)..(/()(:>)8))).)).:'.:,().688;(6(9((((,(,(,(((,,,4(+((+(
@HWI-H225:69:CBJ27ACXX:8:1101:1973:2165 1:N:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATATCGTATGCCGTCTTCT
+
?FFFIFFFIDFIEFIA;FFIFA;=CF@DDDC@C6;?BAABABAAB>AA?3<B5<@:(+42(83((+)5<??A:
@HWI-H225:69:CBJ27ACXX:8:1101:2223:2201 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTTACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
F::BD?GFFFCFDIIIFF>@6@FF@EDCEEF>=)=?BB>?C?5--;A5(,5(,>')0+84:(((:(((+:20&
@HWI-H225:69:CBJ27ACXX:8:1101:2416:2133 1:N:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATT
+
GFF<?99B48?)BDFACD9*0?8B=BFFGF=@DC=DD@D>())7???B;?A(>>@AAAA>;?2=B><((+2((
@HWI-H225:69:CBJ27ACXX:8:1101:2500:2228 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTGCAGTCACGGCCTGATCTCGTATGCCGTCTTCTCTTTTAAAAAAA
+
11?9DD*)6?6DD)988BD:.2=@CCA:)7;7.=?D66?@6>A;;55?,((5(:2'&((:4(((+(((::390
@HWI-H225:69:CBJ27ACXX:8:1101:2643:2042 1:N:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
DBHIJIJJIJGGIJGIGIIJJGHHIJJJIIJJHFD>A;=?5<>ABDDDBDBCCC:@CDDDBBB?<<C8?<<<?
@HWI-H225:69:CBJ27ACXX:8:1101:2570:2150 1:Y:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGGCCTTTTCCTG
+
B=AAABAABBBBAA=AA<6A>9=AA>A;;>=>@>@;@@>5;;=6/:;255::3:8+23+((&&&)0(++((+(
@HWI-H225:69:CBJ27ACXX:8:1101:2704:2150 1:N:0:GGCCTG
GATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGT
+
CDDEDDD=DDDDCDBBCDCCCC@;;:=?9@ADD@>@??AAAD?A????A???>>AAAAA>??>;<<3429<92
@HWI-H225:69:CBJ27ACXX:8:1101:2654:2169 1:Y:0:GGCCTG
CCAATTGTGATGTTCACATAAGGCAGTCCGCAGCTTGCTAGAACTGAATCCCACTTAAAAAAAAAATTCTTGT
+
(1(.<9)0.)>8=8>?1).)0.))(()).-';'-)))(.).(6((.:7((((((5(7(:(())8:-(((((+8
@HWI-H225:69:CBJ27ACXX:8:1101:2568:2170 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTTAAAAAAA
+
1??<<?DCGHFD>;BFGGHG=CG;CG7DHECC>ECD89<A;??5;A@?(8A?ACB@B?@CC<@::(++:>:>5
@HWI-H225:69:CBJ27ACXX:8:1101:2970:2098 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTCCCGCTTCT
+
0=ABA;AAAA:ABBBA)>ABA<;:>@@>;5;<;;;==??=995:;???=;;;9;3:338+(+((+++&)5508
@HWI-H225:69:CBJ27ACXX:8:1101:3156:2163 1:N:0:GGCCTG
CTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTCCTTGA
+
?FGGIHFHGA<?FFFGIIIE<FCFG<6@?=EH>?@?DE.>;@?;>>28(,,>A9,8><>CB9&28>((+++(+
@HWI-H225:69:CBJ27ACXX:8:1101:3112:2193 1:N:0:GGCCTG
TTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATATCGTATGCCCTCATCTTCCTTAAAAAC
+
)7?BFFB;FA;C<(6=.6?2A66?B7>666;A@BBBBBB=@88?((++83+3(((((+((+(+4(4(+(4(+&
@HWI-H225:69:CBJ27ACXX:8:1101:3461:2082 1:Y:0:GGCCTG
ACACGTCTGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCC
+
B5?BA:AB=BA1AAAAAB=BBBBBBABA;AA;>>;;>@=====??AA=???>59==;9;;7(338:;(+97;=
@HWI-H225:69:CBJ27ACXX:8:1101:3388:2103 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCCTCTTCTGCTTGAAAAAAA
+
1?AABBBBA@;AB>BB>A9:AA>ABABB9AA>A>@@@@@===?3;==:85,((9(((+((+(+33+(((+(&&
@HWI-H225:69:CBJ27ACXX:8:1101:3369:2111 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTTCTTTGAAAAAA
+
FHIIGCFGFDGHIHIIIIII@G=FGIGIGHGHCHHD6B<B?=?;@CC95((9:@'&)(+(((+(4(++4:4<9
@HWI-H225:69:CBJ27ACXX:8:1101:3609:2237 1:N:0:GGCCTG
ACACGTCTTCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGT
+
>GECDGIIBDBFFAB<BBFFE=@FEDCD>A)?A,59;@CA>?B;?BB@>BB@B@AA>AB:<9005??::A>B<
@HWI-H225:69:CBJ27ACXX:8:1101:3904:2092 1:Y:0:GGCCTG
GCAGATCGGAGGAGCAGACGGCTGAAGTCCAGTCACGGCCTGCTCTCGTATCCCGTCTTCTCCTTTTAAATTA
+
(-/3())63=''(-69)899'33'8;(,:8=(,52=5&)552((((+&((+2:52+(+86(((+8(+((4((+
@HWI-H225:69:CBJ27ACXX:8:1101:3918:2107 1:Y:0:GGCCTG
ATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCTGAAATAGTATTCCGTCTTT
+
9;=7>;?835=9=3;=?<=?55(,9(39;;<<<(7;:=:<</8528/8(0((+26:((2((+82(+:/((+82
@HWI-H225:69:CBJ27ACXX:8:1101:3947:2160 1:Y:0:GGCCTG
TCCATGGGGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATG
+
.=7:<17(95<''6)8;).6>;8.;>;?8:>187696=;(((--;(;<9;;:=<=(((,,++5:55&((+8(3
@HWI-H225:69:CBJ27ACXX:8:1101:4009:2077 1:Y:0:GGCCTG
CCACACGGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGAACCAACAAGAACAAGAAAAAAAAAAG
+
@1@((-(0((<(7..)9=)6>8:6>.=??06056)6=9)).6=3=;1(-,9/;2;<7<<02(((+(()5:::&
@HWI-H225:69:CBJ27ACXX:8:1101:4026:2090 1:Y:0:GGCCTG
CCCGATGGGTATGGCACAACTCTGCACTCCCGTCCCCGGCTCATGTCGGATGCCCGCCCCTTTTTGTGACACA
+
00=4:''7())))).)=)((7=7)...;((69;5''(',35&((+((+&&+&+83&&0&)2(533&(+338(3
@HWI-H225:69:CBJ27ACXX:8:1101:4041:2120 1:N:0:GGCCTG
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGGATGCCGGCTTCTGCTTGAAAAAAAG
+
?CFEEGII6?@B8?D8)=<BF;C@CDHGEGCEF3)59BCC1;(-55=(','9:-0-&03::(+:(+++4805&
@HWI-H225:69:CBJ27ACXX:8:1101:4116:2164 1:Y:0:GGCCTG
ATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTCCCTCTTC
+
>AAABBBAAAA@@;>@>@@@@A===9;(99======<??=?????????=;;=393(48((+23(+8((++8(
@HWI-H225:69:CBJ27ACXX:8:1101:4035:2245 1:Y:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTTCTTG
+
AAAAABABBBBBBBBAAAABBBA=ABAAAAAA@@@@@@@@@@@========>??=+28+(8&&)2983(+(5(
@HWI-H225:69:CBJ27ACXX:8:1101:4431:2081 1:Y:0:GGCCTG
GTCAGATCGGAAGATGACACGTCTGAACTCCAGTCACGGCCTGACCTCTTAAGCCCTCTTCCGCTTTTAAAAA
+
7=.3)9):3(373())/)6=3.6=/>87=8)8==363','33(8(,:0(,:(((,((+32+(&&&)+(((+(3
@HWI-H225:69:CBJ27ACXX:8:1101:4372:2201 1:Y:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGGATTCCCTCCTCCTCCTT
+
<?5=;?;.96=>8>?<86;1=>==?;???;6)6=88;>?=;<>;75;;;/4+8:<(&+&+6((((5(((((+(
@HWI-H225:69:CBJ27ACXX:8:1101:4416:2240 1:Y:0:GGCCTG
CCAGATCGGAAGAGCATACGTCTGAACTCAAGTTACGGCTTGATCTTGTATCCTGATTTTTACTCTTGAAAAA
+
(():7<365(((((9))/3(.(/();..6.<).)66('''''(4;>((7(7(7(5,2;==(,+(+++(((5(2
@HWI-H225:69:CBJ27ACXX:8:1101:4728:2051 1:Y:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAAGTCCAGTCACGGACTGAACTCGCATACCGCTCTCCTGCTG
+
EICDDD<B@B@AD9B@DB68()8(@@5.=C77?).77@?(.6@@8'9;9((((5(&)+((3)0&)+(((((((
@HWI-H225:69:CBJ27ACXX:8:1101:4574:2136 1:Y:0:GGCCTG
TTAGTAATAGATCCCACACATAGCATCATAGAGTAGTATGGCAGCCTCACCACACAAAAAAAAATCGATGTCG
+
BBBB=::7A<??AABBBAAAAABAB?ABAA>B9>BB87>8ABBA<AAAA=>@@@@@@=====;;78(((((+2
@HWI-H225:69:CBJ27ACXX:8:1101:4595:2142 1:N:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
<FGHFBHCHHGGIIIIIIIIIIGGHGGGHIGGADAAA?=5;=><A=B?@B@CCA:@A?A<?<5<A9(29?@8<
@HWI-H225:69:CBJ27ACXX:8:1101:4822:2086 1:Y:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
:@BBBBBBBBAAABBBBBBBBBA>ABBABBAA@@=;?;::;;>===<9<<=89;????19+9091888;<;<<
@HWI-H225:69:CBJ27ACXX:8:1101:4981:2100 1:Y:0:GGCCTG
ATATAGACTCGAGTCAGATCGGAAGAGCACACGTGTGAACTACAGACACGCCCTTATATCGTAACCCCGCTTC
+
.0(-9)))8)6(-)...)).6=/9'9((359;37(,(7,(5(7+(+27;)&+0&(+(((((+(+(555&&))&
@HWI-H225:69:CBJ27ACXX:8:1101:4780:2175 1:Y:0:GGCCTG
TCAAATCGAAACAACACCCTTATGAACTCCACTCACGGCCAGATCGCATGATATTTCCTATTCGTCTACACAA
+
A*1*0*:))*0)9(97A(7())./77A)=3.))7>@('9'',,(5(((8(((,((488:8;:;3+(898;3:<
@HWI-H225:69:CBJ27ACXX:8:1101:4914:2186 1:Y:0:GGCCTG
AAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTTTT
+
A<A:=;A;;27..(66;/(.=..;5((-,,58'(,(:98()+2(28:?3:;=3;;=?&055-2338;;88(8(
@HWI-H225:69:CBJ27ACXX:8:1101:5005:2064 1:Y:0:GGCCTG
GCTCTTCCGATCCGACTCGAGTCAGATCGGAAGGGAACACGTCTGAACTCCAGTCACGGCCTGATTTTTTGTG
+
)))0)*0:==1(0(('-'5=4==7)7)766;;=(',(,(56(93;>?93(+3(+33(&5;9)2((++((&&(+
@HWI-H225:69:CBJ27ACXX:8:1101:5057:2096 1:N:0:GGCCTG
CGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTTTGACGTCC
+
1?D;?BFHGDD.(='=CFGA=;@AGEEBDEE(;;/;=;?CCC@>>:(5:A:;<-0&++4>@@?+((((()+)+
@HWI-H225:69:CBJ27ACXX:8:1101:5159:2135 1:N:0:GGCCTG
GGCAAGGCTTCCCTGCACCTCTAGCCCAGCTCATCCTGCCAGTCAGCCAGAAGCACCCCCGCCCCCCACTTCC
+
EEIIIIIIIIIIIA>DDIIIIIIIIIIIDDEICEEEIIDDDCADD>ADAAAAAA>?;????>?>????>?AAA
@HWI-H225:69:CBJ27ACXX:8:1101:5157:2186 1:N:0:GGCCTG
CGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGTCGTCT
+
?CBDCDEIII7DDDDEDIE>CCDA;AD@@AADDD@@@A=A>AAAA;>A>AA??=??>>>(+42<(9(++28<<
@HWI-H225:69:CBJ27ACXX:8:1101:5386:2148 1:Y:0:GGCCTG
TCAGAACGGGAGAGGACCGGGCTCAATTCCAGTCCCGCGCTAGAGGCGAAATTCTTGGTCCGGCTCAAGAAGA
+
<3..)0)'-'-3=((..(',',,(7,,(((7(,(((&&)06))(+(()&0&(+8(2(((+()&))&(3+6(((
@HWI-H225:69:CBJ27ACXX:8:1101:5372:2165 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTTACGGCCTGATCTCGTATTTCGTCTTCTTCCTTTGAAAAA
+
=?3=><???<=4<=??(:7=(.<9;1)3=;;9>1=8:=?==573(73,,,(((,(,((7((+((+((((((+(
@HWI-H225:69:CBJ27ACXX:8:1101:5676:2119 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCCCCTTCTGTGTGAAAAAAA
+
ABBAB<?BA;AA55==/=A:86>ABBBBBB;A=)).99<<;5(5;?;((5=((5((0&8(3(((+(,3:=()&
@HWI-H225:69:CBJ27ACXX:8:1101:5693:2150 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAAATCGA
+
CEEEB:CD=;DEIDBBDDEIIDDEEEDIICEDICAEADCDCCDDDDDDDD@@@??=???A??????>??<:08
@HWI-H225:69:CBJ27ACXX:8:1101:5836:2060 1:Y:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAGAAAAAAAAATC
+
<ABBA::=?AB>A=)093=AAA=AABBB>=A>ABBB>;=;AAAA>3>@>@@@@====>>?====9====;=<=
@HWI-H225:69:CBJ27ACXX:8:1101:5798:2213 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCCTCTTCTTCCTTTAAAATA
+
FGBAEEIIBGIIFIIIIFIB=C@1=CDEEFE>)).;9B?>;??BBB;58,,5:A3(23>::(((4(+((:(((
@HWI-H225:69:CBJ27ACXX:8:1101:6166:2067 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCT
+
=7;=AAA==@(3.66>A>;>??<89??A=9(0+89<AA:>4>A:4:>:095>;8<+(4:>8<33(455;(+3:
@HWI-H225:69:CBJ27ACXX:8:1101:6030:2088 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGCATGCCCTTCTCTTGTTCAAAAAAA
+
FDGDF9BF:DFGIAGECBFG2@@CGIIGFHEE7?CE/9/;;?;5@5>((,85:>9((+44((((+((+(+2)0
@HWI-H225:69:CBJ27ACXX:8:1101:6217:2227 1:Y:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAAATCGA
+
BBBBAAAABABAB)=?AA>?BBAABBBBB9ABABBB?BA377AAA>@>;==@@==>>>>3==:=;;;;==?09
@HWI-H225:69:CBJ27ACXX:8:1101:6210:2243 1:Y:0:GGCCTG
AGAGATCGGGAGAAAACAAGTCCCAAACCCAAGCACCGTCGGTACTAGCCTTGGGGAACCACCTACACATGAG
+
(((:=?1(((7''()))6()8=)).=.(9?3(.(((,'('',&()++(35::((+82856;<<;;5:((+(5(
@HWI-H225:69:CBJ27ACXX:8:1101:6335:2089 1:N:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGCC
+
<FHGHIIIIIEIHCGGIIGIIIGCHHIIIIIIEDB;>;??@?C2<;<@?<5+9:@CCCBB<?5<89ABCBB&+
@HWI-H225:69:CBJ27ACXX:8:1101:6352:2243 1:Y:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
AEEEIEIIIIIDDEDE9BDCE<BDDIIIIIAIDD@?>???D?A?????<>;>>A>>AA>>>?-<(9><>?><>
@HWI-H225:69:CBJ27ACXX:8:1101:6646:2032 1:N:0:GGCCTG
TCTTCCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCCTCTT
+
IHIIID@FHGIEGGDG7=DHHDABDBDCEEEEB@BBCBCCCCCCCCCCCCBBBBCC(4>CCA<?33@@(4<CC
@HWI-H225:69:CBJ27ACXX:8:1101:6542:2080 1:N:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATG
+
CCGEFAE?DDGCGIFFI9BAF<BCFFICFFAFEFIIEIE;?CED@BB>A@BCCCB:>A@AB9?@B<@@><?8:
@HWI-H225:69:CBJ27ACXX:8:1101:6538:2142 1:Y:0:GGCCTG
ACTCGAGTCCGATCGGACGAGCCCACGTTTGCAGTCCCGTCCCGGCCTGGGATTTCATTAAGTCTTCTTGCTG
+
1***)111:*0)00(.-7:'5='(.(,(((,(((((-9;=;''&0&)&+(((+(+(+(((+((4(((+(++33
@HWI-H225:69:CBJ27ACXX:8:1101:6536:2167 1:Y:0:GGCCTG
ACTGGAGCAAGAACGGAAAAACAAACGACTGAACTACAATCACCCCCTTGTCTCCAGAGGCCTCTCCCTCTTA
+
:(-((..)(0).)9(((-').'..(;5'53':3(:(:((((--,,8<<5(:<:<5(((++(++2((++(8(((
@HWI-H225:69:CBJ27ACXX:8:1101:6734:2181 1:Y:0:GGCCTG
GTCCGATCGGAAAAGCACACGCCCTGTTGGCTCCAAGAGACTCGGGAGAAGACAACAACACAAAACAAATAGA
+
(0))(0-(-<3=')..)))(''(',6(.6(7((((((,,(,(,,(&)5&((8622(2((+(+((+((+(((++
@HWI-H225:69:CBJ27ACXX:8:1101:6865:2023 1:Y:0:GGCCTG
CCAGCTTGAAAATGAGGGGGAACCTTCCCAATAAACACGATTTTGATATAAAAAAAAAAAAGAAGTCGACAAG
+
??):)*:?)**:=A?A*7<A'-5()7==9).7)6553==35;((5(:93(5;>9;99;;&&)8((+(+855&(
@HWI-H225:69:CBJ27ACXX:8:1101:6882:2044 1:Y:0:GGCCTG
CCGCCGTGGGAAGGGGCATCTCTTCTCGGGCTGAACTGGGCCCTAGACTCACCAACATCAAGGACATAAAAAT
+
23((0(((0'()((''''..)8).).)(('355(8(,,(,(()+/(6:(66(26((((+(((++(((+(((+(
@HWI-H225:69:CBJ27ACXX:8:1101:6899:2081 1:Y:0:GGCCTG
TCGGAAGAGCACACGTCTGAAGTCCAGTCACGGCCTGTGATCGTCTGGCGGCTTGTGGCTTGCATTCAAGGGT
+
:1=;=5=16:).973.6=5>>)..):)):=9=;=8'((-(,7(,(((,()&&&0&(+((+(&(++((2;:<2+
@HWI-H225:69:CBJ27ACXX:8:1101:7062:2022 1:N:0:GGCCTG
TACTAATTACACTAATCGCCACTTCTATAACAGCTATGTACAGCATACGAATCATTTACTTCGTAACAATAAC
+
IEFIIIIIIICGIIIIIEFFEIFFIIIIIIIF@EFFFFFFFE:;BBD@>B?BBBBEDDA@BBB@B?@BBAB@>
@HWI-H225:69:CBJ27ACXX:8:1101:7019:2092 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG
+
IIGIIIIIIDHGHIIBCHHIH9CFHIIHIHGHHDCCB>>@CDB=?C?AC;8=1<>:(4(4((+:A85&)&&((
@HWI-H225:69:CBJ27ACXX:8:1101:7003:2227 1:Y:0:GGCCTG
GTCAGATCGGAAGAGCACACGGCTGAACTCCAGTCACCGCCCGGTCTCGCTCCCCCCCCGCTTCGTGTATAAC
+
)1?AA*0:?4)8=>5).(.>-'-':>A=);).7?@>;((99',',5&83((()+)&)0&&&&)&&+(+((((3
@HWI-H225:69:CBJ27ACXX:8:1101:7452:2071 1:Y:0:GGCCTG
ATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGG
+
BBCBBBBB>BBBBBBBBBBBBB?AAAAAAAAAA<A@@@BBA@B@AA??====>?==?A?=????????????=
@HWI-H225:69:CBJ27ACXX:8:1101:7440:2084 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGGCTGAACTCCAGTCACGGCCTGATCTCGTATTCCCTCCTCTGCTTGGAAAAGA
+
=)6):3=>?93=77<>??93'53:6=;)9=;0).68;=8;;;<(:==((,((+3((6(2(((+3(+(((++((
@HWI-H225:69:CBJ27ACXX:8:1101:7493:2126 1:N:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
CGHGAAGGFCF?DEEB:DDGGGIEGHDCGFEEEB<@@?BBDD3<A@BBBBB>3:@>C9?B?79<<3:A<8912
@HWI-H225:69:CBJ27ACXX:8:1101:7491:2145 1:N:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
IIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIHFCBCBCCECCCCCCCCCCCCCCCCCCCCCCCCCCCCCBAB
@HWI-H225:69:CBJ27ACXX:8:1101:7563:2104 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGTAAAAGA
+
)86;3<???9;6>9><<<?8763=?=>>?;?;6;;835===9;3--:(,(7;=5'3388((3:2;(+62:+((
@HWI-H225:69:CBJ27ACXX:8:1101:7722:2114 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGTCGTCTTTTGCTTTGAAAAAA
+
*?<A9A7?AAA6/(/..8=:2==->>)73777=.>),336=(5;((,(,,(5(5,2((2(((+(5(+38=?(5
@HWI-H225:69:CBJ27ACXX:8:1101:7532:2131 1:N:0:GGCCTG
GATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTT
+
HJFIJJJJJGGEHIFGHHHGHIBGIEEEDFFFFC@AD@CCDCCCCCACDDBDDDB??A@CDD?BD<CC>9BBB
@HWI-H225:69:CBJ27ACXX:8:1101:7535:2174 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTTCTTGAAAAAAA
+
IIIIIFIIIIIBFIBFIFIFFFFFFIIFEFFEEEFDDDC@B=?>ADA9?@B?BB')0<?@B(:4:((+4><>B
@HWI-H225:69:CBJ27ACXX:8:1101:7660:2179 1:Y:0:GGCCTG
CTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGGCTTCCGCGCGG
+
E>D@@D9D<?B*BDA=;ACCDE=ACA6=3?CDDADDD;6@@@@??/,8(,,(55((++44)&)&&((&&&&))
@HWI-H225:69:CBJ27ACXX:8:1101:7772:2068 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGACCTCTGATACCGGCCTTTTCTTGGAAAAAAAACAA
+
<;8::;8)9;9BDB68B=(.=;4==CCEIDD6<'9<>A-5;>(((-((5,'')0(((4(((+(+:((&)&&((
@HWI-H225:69:CBJ27ACXX:8:1101:7775:2137 1:N:0:GGCCTG
CTCTTCCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCT
+
IGIIIIIIIIIIIIIIIIHHHHEDEECCCCCBCBB?BCBCCCCCCCCCCCCBBBBB@C>:AC??@C<>C909?
@HWI-H225:69:CBJ27ACXX:8:1101:8173:2032 1:N:0:GGCCTG
TTAGTAATAGATCCCACACATAGCATCATAGAGTAGTATGGCAGCCTCACCACACAAAAAAAAAATCGATGTC
+
JJJJI?CDHHHJJJJJJIJJJJJIIJJJJJJJJHIJJJJJJJJIGGHGFFFFDDDDDDDDDDDDDA@?B@CBC
@HWI-H225:69:CBJ27ACXX:8:1101:8210:2065 1:N:0:GGCCTG
TGTAGACTCGGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTG
+
DHG@GGEGIIFCHEECECCCCBB@?CC@CABBBCB@CCCCCC>C:@A95<>B094::CB0<33:>&)52<(4(
@HWI-H225:69:CBJ27ACXX:8:1101:8250:2094 1:Y:0:GGCCTG
ACACGGCTTCTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGACACGGCCTGAACTCGTTTTT
+
?)??4))7:)))7)-5'5;'.==)=)7(5(59((,5(((,,((,(,,5(388(+8())&)&+838(+(+(2+(
@HWI-H225:69:CBJ27ACXX:8:1101:8171:2096 1:N:0:GGCCTG
TCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTC
+
EGFGIDHIJJJIJHHHCBDDFDCDDCD@?ACDDDBBABDDDDDDDDCCCCCCBBDDDD=@CDCB<@CACCB59
@HWI-H225:69:CBJ27ACXX:8:1101:8287:2109 1:N:0:GGCCTG
CGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCT
+
F@3?*?FAFAGD;FGCFGG48=;;@E/?6?7ADDB;?;=A>;ACCC>;CAC8;=9@+3(4:@(+2>((4&))8
@HWI-H225:69:CBJ27ACXX:8:1101:8344:2124 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGTAAAAAA
+
GGIEFGIGEHHHIGIG=BCB2@@CAHIDHIGH?EHDD<8A@;?;>CC??BCCC>8&08?(4(43>(+(++4>.
@HWI-H225:69:CBJ27ACXX:8:1101:8380:2226 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCT
+
?=>8:>?=????>7::<9>>?=;=??579>??>>9;;?;>>>><=<<>538;;;<2(32+(8;6:/85:3:;:
@HWI-H225:69:CBJ27ACXX:8:1101:8399:2235 1:Y:0:GGCCTG
GCTCTTCCGATGTCGACTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTCATCTCGTAT
+
3355=.7=??159)97;69=?5'3=6=(6.;33;7,(575;/+69-:5:2::::(62:9958:(((36:89(8
@HWI-H225:69:CBJ27ACXX:8:1101:8650:2129 1:Y:0:GGCCTG
TGGGCTCGGAAGACCACTTGTTTAAACAACTGACGGCGCGCTAGGACGGGAATAGCCTTAGTCTTTAGAAAAG
+
1-((0)(7((-'().86.).))).)))).())).,3',,0:-5&((()0)&&+(+((++(++(((+5((365(
@HWI-H225:69:CBJ27ACXX:8:1101:8735:2209 1:N:0:GGCCTG
GCAAAGTCAGTAGCAGCAGCAGCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTG
+
GHGIIIBGGG<FDHHIGIEEHEDEHGIHFDDDD?:8AADBCDA@<@@D@:A@ADCDDDDB3A<C@BD<98??8
@HWI-H225:69:CBJ27ACXX:8:1101:8690:2218 1:Y:0:GGCCTG
CACTGAAGAAAAAAGTCAGATCGGCACACGACACGTCTGAACTCCAGACACGGGCTGACCTCTTACTCCCGGG
+
1**1****0*0))9().9))=;.''-')(,'593'5;(8:;((,5:5(5(3)&&&&&(++(+(++(+(3(&&&
@HWI-H225:69:CBJ27ACXX:8:1101:8831:2070 1:Y:0:GGCCTG
CTCGAGTCAGATCGGAAGAGCAAACGGCTGAACTCCAGTCACGGCCTGAGCTCGTATGCCGTCTTCTGCTTGA
+
?AAA<A0>?<==>A<A;:==)7)855(5:5>>>@@;>(.;;?=3/8==:(88;&&8;98:;09<9?83338((
@HWI-H225:69:CBJ27ACXX:8:1101:9129:2161 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCTGAACTCGTATGGCGTTTTCTTCTTGATAAAAA
+
DEIGHBHII=FF@D@9==FG2@8;CECDGIBHA>EC);9<=?=(-;AB<??((5&)&)8::(((4(((+(>>(
@HWI-H225:69:CBJ27ACXX:8:1101:9061:2174 1:Y:0:GGCCTG
TCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCATT
+
1=5;;).:=>8=55=8?0=3=?(9//;58;(75;86;<<5:</26:;/82:2&&05528(3(+8<85((5(+(
@HWI-H225:69:CBJ27ACXX:8:1101:9410:2016 1:Y:0:GGCCTG
GCGAGTCAGATCGGAAGGGCCCAAGTCGGAACTCAAGTAACGGCCTGAGATCGAATACCCATTTCTAGTTAAC
+
'''-5'(619.6('(9'(,(('++6:((&0()2((+((((((&0&&++(+((2(++((((((5(++((+((((
@HWI-H225:69:CBJ27ACXX:8:1101:9492:2018 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAAAAA
+
DHIIIII@GEGAEHGBBFHIIGIIIIC@EHHEFFCBBCCCCCB1=CACCB:@?8:4(((4(++:::550500&
@HWI-H225:69:CBJ27ACXX:8:1101:9494:2040 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAG
+
CEFHIIGGG@BGHGG@;FAFGCF9BF78.@FEBEEBBE@7@ACBCCCCC@8?98@C@C:>(84@CBB)&))((
@HWI-H225:69:CBJ27ACXX:8:1101:9370:2091 1:Y:0:GGCCTG
CCTACCAGAAGCACGCAGGCGTCCAAGAACTCAGTCCCGAAAGCTGCCTTTGCCAGCCAAGGCTATCCAAGAA
+
(((1.())3))))0((0-''--''(.)().)8)6.8(.''',,(,3(,,(,((+3:322((+((++(+332/(
@HWI-H225:69:CBJ27ACXX:8:1101:9494:2127 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
DHIJIJJJJJJIGGIGGHIIFHFHGGHGHJHGHGHEFFCBBB?@CDCA8<AACD2&08?@:::4@(+:AC?>B
@HWI-H225:69:CBJ27ACXX:8:1101:9497:2156 1:Y:0:GGCCTG
TTAGGAATAGATCCCACACATAGCATCATAGAGTAGTATGGCAGGCTCAGCACACAAAAAAAAAAACGATGTA
+
1.>?)<.7=?>8):3;>9=1>)91=)<)6;;?@))>;)):=6):''9;0(.9;77((75;75&0:0&&)(26(
@HWI-H225:69:CBJ27ACXX:8:1101:9432:2183 1:Y:0:GGCCTG
CTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCCGATCTCGTATGCCGTCTTCTCCTTTT
+
4?=66=:<<10:>>;?931.).(:(-(63(<=;??;;0.=;='395''0&)(5/+23+(:89:<26((((+((
@HWI-H225:69:CBJ27ACXX:8:1101:9683:2055 1:N:0:GGCCTG
CGGAAGAGCACACGTCTTCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCC
+
JIIJJIJGAFHIIHIGIDGIHIJJJJIHIJJID;AEEFDFFF?C/??CDDDDD<?A?@ACDDDDC@CCC9>@B
@HWI-H225:69:CBJ27ACXX:8:1101:9732:2162 1:Y:0:GGCCTG
GCAGATCGGAAGAGCAGACGCCTGAACTCCAGTCACGGCCTGACCTCGGCTGGCGCCTACTCTGTCTCACCCC
+
(--=A8ABA7;4A>@;);2,'3(;;95;?:5;(5(:&&55&+++3(((&(+)&+&&)&&((++(+(+4((((&
@HWI-H225:69:CBJ27ACXX:8:1101:9634:2219 1:N:0:GGCCTG
AAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATG
+
IIIIIIDDDAE@ADDDDDD>@A@A>A>A????????>=<??<?<?AAAAAAAAAAAA?>>?>>3+:4>82<A9
@HWI-H225:69:CBJ27ACXX:8:1101:9609:2230 1:Y:0:GGCCTG
TCAGAGCGGAAAAAAACACGACCGCCCTCCAGTCAAGGCCGGAGGTGGCAAGGCCCGGGGCGCGGACGAGAAA
+
(9).).)-'5'')(,''(('','&&&&)+2(+2+(((+(+&)&)&+6+((+++((+&&&)&&&&&&&)&&)&3
@HWI-H225:69:CBJ27ACXX:8:1101:9898:2121 1:N:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAAACCAAG
+
IIIIIJJ@FHGIHIGIJBGHJJHGIGGHIJIJFIIIIHHEEADFFFCC>A>CCCDCDCCDDDDBDDD.&(+((
@HWI-H225:69:CBJ27ACXX:8:1101:10171:2020 1:Y:0:GGCCTG
TCCGGTCAGACGAGAAGAACACGAAGCTCGAACGTCTGTAATCTCACCACGGCCGCGTCCCCGTCTGAACCCT
+
((.-((0()9)-';'-))))))',,,'(6''(('(,(,(((,(((((((((05&&&&&&)&(&)+((+(56-(
@HWI-H225:69:CBJ27ACXX:8:1101:10168:2207 1:Y:0:GGCCTG
GTTTAGAATAGAGTCACTTCAAAAAAGAAAAAGCTTGAAATCCACTCCCGGCCCATTTGTATAAACACTCTTT
+
(15(0).))0<)7:99...)/).).5-3>79('()).((.:-((-((,(,820)+(((++(+(((+++28(((
@HWI-H225:69:CBJ27ACXX:8:1101:10362:2033 1:N:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGGATTCCCGCTTTCTCTTT
+
EIIIICDDDEEEDDADIIADDICCDDAADDDDDDDDDAABAAA????=??AADA2()()(4(&&&)(((((4(
@HWI-H225:69:CBJ27ACXX:8:1101:10467:2050 1:Y:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATA
+
B@BDD?D??86D?D>8//=8BCB=A7@C;8=7@C)=7A?6?AAA;;>@A@:>AAB:33;:??:59?>8((22(
@HWI-H225:69:CBJ27ACXX:8:1101:10288:2078 1:N:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATG
+
HIGIIIIFHHHIIIIIIFIIIIFHIIIIIGHIIIIGGEHEHCHEEEEECCCCCCCCCCCCCBBBBBB848?1>
@HWI-H225:69:CBJ27ACXX:8:1101:10444:2100 1:Y:0:GGCCTG
AGGCAGAGGCAGATGGATCTCTTTAAGTTCAAGCCTAGCCCGGCCTACCTAGCAAGTTTCAGGCCACCTAGGG
+
)?5=1?ABAAAAABB>=<A7=====B>.>BB;7)-=77=A96/'39;==;5;???=????9;?=8;9;=?33(
@HWI-H225:69:CBJ27ACXX:8:1101:10439:2149 1:N:0:GGCCTG
CTCTTCCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCT
+
BHIHGG4;AG<BGEE77?>>>@6,;??9;=CBCB=BB<5>@CAACC@4>AC99B<9?CACDCAB??<CC&002
@HWI-H225:69:CBJ27ACXX:8:1101:10582:2034 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTTCTTTAAAAAAAAAAAA
+
IIIIIIGICGGGIIGCFGBHHIHGGHHAEAHEEBDDDC;@@CB(5>(,>;38<?>4((+4+(+(4:5&)5&&0
@HWI-H225:69:CBJ27ACXX:8:1101:10544:2056 1:Y:0:GGCCTG
TCGAGTCAGATCGGAAGAGCACACGGCTGAACTCCAGTCACGGCCTGATCTCGGATGCCGCCCTCTGCTTTGA
+
;)?=?0907AAB=A57<(8;3>.=:/:/9;5;>@>>5(5-;=:==6=(+((+((08&230&)&&(+(((+(((
@HWI-H225:69:CBJ27ACXX:8:1101:10656:2066 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCCTCTTTGAAAAAAAAGAA
+
IIIIIIFCEGIIEGIGG9FFF=CGHIDEHHFEBDAAA>>C@C?1,,((5',3+8(((((4(((4::8&))&(+
@HWI-H225:69:CBJ27ACXX:8:1101:10667:2207 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCCTTGAAAAAA
+
9C?DDDE;DDDDBBBDCDDIACCCEECEEIDCCDDDDD@@8;55>>>985:((52)0(8>((4(+(+4+4310
@HWI-H225:69:CBJ27ACXX:8:1101:10500:2233 1:Y:0:GGCCTG
CCTGATTGAAGATGAGTGGGAACCTTCCCAATAAACACGTTTTGGATATAAAAAAAAAATAGATGTCGACTCG
+
EIBD3CDDEIEEIE>D*?B;DD6;;)=CACE37@CADDA>>C=(';;>>ADDAA????><>>AA(+(++08&+
@HWI-H225:69:CBJ27ACXX:8:1101:10825:2223 1:Y:0:GGCCTG
TCGGAAGAGCACACGTCTGAAATCCAGTCACGTCCTGATTTCAGTTACCTGCCTTTCTTTTCTACAGTTTTCT
+
91(((5(((0<(3=5(.7()9)7=>;.):=??.:=-=93)).))..(.(.(((-((-(,,(((((((((((,6
@HWI-H225:69:CBJ27ACXX:8:1101:10957:2248 1:Y:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCATATTCCCGCTTCTTTTTT
+
AEIDDDI3BDE>4B;0?B:=CD@CC=@@CA=A>7;;ADD@AAA/93;?<9(:;>(((((++(&)&)&(+((+&
@HWI-H225:69:CBJ27ACXX:8:1101:11089:2074 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCT
+
??>?<??<????????>?<><?;?:6>><??8.==9>???>><<=<==<7<552(8<<=32(++(+5088<53
@HWI-H225:69:CBJ27ACXX:8:1101:11221:2080 1:Y:0:GGCCTG
CGCTTCCCCGCCGCTGCGGCTCCAAGGCCTACCACCTTCAGAAGTCGACTTGTTGCCAGTGTGGCTACCCTGC
+
<(<=-().7-'59'''',9/3;350365/(6:8</++8+66:65+(85:-6(((+8(:(((46((22/87;7(
@HWI-H225:69:CBJ27ACXX:8:1101:11208:2163 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAGAAAAAAAAAAT
+
IIIIIIIIGGGIIIIEHGHGIEEGGAFIGIIIIGEFHGGFFFFFDCABECB@BBCCCCCC<?C<ACBBBBBB8
@HWI-H225:69:CBJ27ACXX:8:1101:11249:2184 1:N:0:GGCCTG
GTTCTAGCTACATCGTTCTAGAAGATATGAGTTAATCTCTAATAAAAAAAAATCGATGTCGACTCGAGTCAGA
+
J:CGGIEIJJFIHIFIJFHGDIFGGHGICGIHHIIIJJJJJJJBHIGG>CDDDD@BB>BCDDDDDDB@@CDDC
@HWI-H225:69:CBJ27ACXX:8:1101:11176:2202 1:N:0:GGCCTG
GACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTT
+
JIFIIJIIHIGHGIIGIEHHIJDDGGIFAHHFDFEFCEDCEECDDDBD@??@>@@B@BACAC@<@BDDC:AA:
@HWI-H225:69:CBJ27ACXX:8:1101:11177:2229 1:Y:0:GGCCTG
CGATGTCGCCTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCT
+
BAABA?AB)=AAAAA).=>8===6;AA2(7=@>>=>@@=;>>????>>:=?==;=;;3(8:?7+9?==:9009
@HWI-H225:69:CBJ27ACXX:8:1101:11292:2019 1:Y:0:GGCCTG
ACACGTCTGAACTCCAGTCACCGCCTGATCTCGCATGCCCTCTTCTTCTTTTAACAAAAATGCTAGTAAAAAA
+
6;>1-=?9=??533=>3.9.<)(-;9(6)<):((,3((6(.,(697((,73(,2(5;3(((+((((+(+2/85
@HWI-H225:69:CBJ27ACXX:8:1101:11365:2100 1:Y:0:GGCCTG
CTCGAGTCAGGTCGGAAGAGCACACGTCTAAACTCCAGTCACAGCCTGCTCTCGTATGCCGGCTGCTCATTGA
+
007((-.)8))..;@'-4?;;76(.=;5=(53((,(((5(5,((++3((:44(52239(()&&&&+4((4+((
@HWI-H225:69:CBJ27ACXX:8:1101:11289:2119 1:Y:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAATAATACGCCATCACTGCAGTTTGATAAGTAGAATAAAAGAAT
+
9@<=8139)).0<<))07:)).99=))76('().)))(''',)..((3(9((((-(-6(,,((,,(((7((,5
@HWI-H225:69:CBJ27ACXX:8:1101:11788:2074 1:Y:0:GGCCTG
CGAGTCAGATCGGAAGAGCCCACGCCTGAACTCCCGCCACTGCCTGCTCTCCTATGCCGTATCCTTCTTTACA
+
.((1)19=93.3(0797?)((.(-'(((863)<3('',,9(7:9((((((,,(,(,(,&))2(((((((((++
@HWI-H225:69:CBJ27ACXX:8:1101:12087:2017 1:Y:0:GGCCTG
TCGATGGTTTTGTTCAGCTACTCGGTTCTGCAGCTTGTTGGGATTGAACCTTTCTGGAGAAAAACAATTGCAT
+
0=(<;?3=)(7(..))/))).))((-5(=>?)=)).((6=(99<(,(((,5,(,,(5((68<6/&+8((+(((
@HWI-H225:69:CBJ27ACXX:8:1101:12107:2021 1:Y:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGGATGGAAGCTTTCTGGGAAAAAAAAATCGAAG
+
*1*:*?*=*)??))=*99***0=***98ABB>>8.>==ABAA<7;();)....66;;,(;;9;25)9(+(((+
@HWI-H225:69:CBJ27ACXX:8:1101:12201:2033 1:Y:0:GGCCTG
TCATATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCGCCTATGTCGCGCTCTGCCTTATAAAAA
+
2=))1>;((<3((..061('.<:;>>1<3=?0.8=9''5,93((((('''7;,(((&&0&+8(+((((+8:<=
@HWI-H225:69:CBJ27ACXX:8:1101:12105:2039 1:Y:0:GGCCTG
CACAGGTATCCAAGTAGGAAAGGAGCTAGGGACCAAAGGAACCATAAATGATTTAATGAGACAAAAACAGTTT
+
):):)(2)).3.11.9=6))0(0(0((0)(.<(<;3(.)().(()).))68=?;.(.(:(.54((,(,78(5(
@HWI-H225:69:CBJ27ACXX:8:1101:12209:2057 1:N:0:GGCCTG
TTAAAGACCCATAACTTCAATAGCTCTGGAGAGTTGGCTGGTTTACCAGTACCAGGCATAATTTCCCTCCTGT
+
EIJJI9FGIIJIGIJJJJJIIJCHIIJIICGG=@DCHEECF>CDCCAEEECCACCDDBCD@@DDC:ACCB<AC
@HWI-H225:69:CBJ27ACXX:8:1101:12163:2167 1:N:0:GGCCTG
GATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTT
+
IGIIGIIIHCHGHIBGIGIFIIGCEHAGCGEECBBDECCCCA@CCC@@;A?BBBB?9::AC8(2>A:@&&)((
@HWI-H225:69:CBJ27ACXX:8:1101:12437:2048 1:Y:0:GGCCTG
GCTGGAGACAAGGGGGAAGACCACAACTCTAATCTCCGGTCTCCCTCCACTCCCTGATCTCCTTTTCCTTCTT
+
(-(((((.-.)0((''-5(.).3(9((63((((-(:(''',&(+((+(((++89+(((+(+((+(((++5(+8
@HWI-H225:69:CBJ27ACXX:8:1101:12404:2063 1:N:0:GGCCTG
CACACTCCTGGTCGACTCTGGGAAGGTGGGAGAAGAACCCAACAGAAACCCATCATCGGCCCCGACTCCTTTA
+
:))10):)*0*00)06((/=@8(8((..--(('())))('(((((-(((,88(,,(+(&&)&&&&)))(:4((
@HWI-H225:69:CBJ27ACXX:8:1101:12350:2087 1:N:0:GGCCTG
TGTCGACTCGAGTCAGATAGGAAGAGCACACGCCTGAAATCCAGTCACGGCCTGATCTCCGATGTCCCCTTTT
+
8487@;81/5(5..))7)).;3.(.5(((,,''((((,((4>3>>((()059&(3+(+4(&&()((+(+9(((
@HWI-H225:69:CBJ27ACXX:8:1101:12493:2195 1:Y:0:GGCCTG
TATGTGACTTTAGCATGTTTAATAATGATGACAATAAAAAAAGCCCTCAAATGGGGGCATTGAACCTCTAAAA
+
?*?4000=?=AB>**=*//)9>)9AA=474>>>7>BAB>>@//,((5=99=?:(((5533+8==?79;=???;
@HWI-H225:69:CBJ27ACXX:8:1101:12359:2213 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
1?4CFGDD<1?<(9?BBFHBDAAF@EC;DHF).?;;98AC8(,-5;><5??3>@'&088@>(4:@((++42)0
@HWI-H225:69:CBJ27ACXX:8:1101:12734:2073 1:N:0:GGCCTG
CGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCT
+
FFBABBG=;DBDF(<)B<CF@F65(;;26=7@@BB;=@@@BBABB:@;@AAB?:@9??(:@A<8<A?>@5&5(
@HWI-H225:69:CBJ27ACXX:8:1101:12623:2081 1:N:0:GGCCTG
CGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAA
+
GGDECGGHFEHAHFGGGE<BFHDGFHCAAHEHEEECCCFFFDDB6??:@@C??<>(,:5008?:4+:@C((4:
@HWI-H225:69:CBJ27ACXX:8:1101:12561:2086 1:N:0:GGCCTG
CTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGA
+
HHIIGGCGGGIIEIIGHFHCEFDDFC>ACBCCCCCCCCCCCC@BBBBB<3>>A8+899::<05ABAC>(:>>8
@HWI-H225:69:CBJ27ACXX:8:1101:12742:2103 1:Y:0:GGCCTG
TGTCGACTCGCGTCAGATCGGACGACCCCACGTCTGAACTCCAGTCCCGGCCTGATCTCGTCTGCCGGCGTCC
+
)*)0)?==A<0=4A'5)7A=5(',''3'8(828880((+8:=:=:((5&55558(((338+(++(+&&)&&&&
@HWI-H225:69:CBJ27ACXX:8:1101:12732:2133 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAGAAAAAAAAAAT
+
JJJIJJJJJJIJJIIGIIJJJJIJIIIIIGIJIAHJJJJGGGHHHFFFFFDDDDDDDDDDDDDDDDDDDDDD?
@HWI-H225:69:CBJ27ACXX:8:1101:12538:2180 1:Y:0:GGCCTG
CCAGTCCTTGGCACCCCAACAAACCTAAAAAACAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
AA351@ABB>B>*0?AAAA<=(85;AA;AB8''(.)),6=>;>@@====9=59=95=,8-283;8;8<78)29
@HWI-H225:69:CBJ27ACXX:8:1101:12845:2014 1:N:0:GGCCTG
CGATGTCGACTCGAGTCAGATCGGAAGAGCCCACGTCTGCACTCCAGTCACGCCCTGATCTCGTATGCCGTCT
+
98CB*0*)0?6???@CAFE4).6855@(.7(56?<66;;((;>;5(,5>:@8(89<((+:+:(+(++44&)0+
@HWI-H225:69:CBJ27ACXX:8:1101:12804:2158 1:Y:0:GGCCTG
TTTGTCTTAAATATGTCGGGGGAGACCAGTTTGGACATGTGAACATGGTGATGGTGAGATCACTAGAGCCCCA
+
0?=ABBBB?B4==ABB?A<AA6<=';=:(,,5,(,58=;38:=8(4838;??=??;((4;??==8:??:<959
@HWI-H225:69:CBJ27ACXX:8:1101:13045:2004 1:N:0:GGCCTG
TCGAGTCAGATCGGAAGAGCACACGCCTCGATGCCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCC
+
8C8)?*)00?*000)(.?B38==A''-=A4-..()'',,,,9'5,55>((9)0<>>>?><><>8<>0:>AA(:
@HWI-H225:69:CBJ27ACXX:8:1101:13019:2080 1:Y:0:GGCCTG
GCTCTTCCGATGTCGACTCGAGTCAGATCGGGAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCTAT
+
A88:AABA64;<(8ABAAAAA@>;>>3>>2='32=8=3;=;;+8+(3+88???(:?3;;-9502+3:??3+(3
@HWI-H225:69:CBJ27ACXX:8:1101:13144:2094 1:N:0:GGCCTG
TCGGAAGAGCAAACGTGTGAAGTCCAGTGAAGGCCTGATGTCGGATGACGTCTTCTGGTTGAAAAAAAAGAAC
+
*?DD))00)?*0*(((/.()))../)88).)7@9==5))))).',('(-,8,((,5:3>383+((2&&&&(+(
@HWI-H225:69:CBJ27ACXX:8:1101:13237:2242 1:N:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA
+
EEE<@DDEDDDDDDD@DDADA88BCDEIAEEC@CE>CADD@?@;;>AA???A<AA?=;<>A:+4>>3(+4489
@HWI-H225:69:CBJ27ACXX:8:1101:13499:2002 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCCTCCCCCTTGAAAAAA
+
GHHEG@FGIDC<FD>?;FB@F28@;CCADGEHEEHDBFCA>@CCC::55?(,(:'55&+((()&)((((((2.
@HWI-H225:69:CBJ27ACXX:8:1101:13333:2125 1:Y:0:GGCCTG
TGTGAACTGAAGCCAAAACGGAAGAACACAAGTCTGAACCCCATCCCCGGCCTCATCTCCTATTCCGTCTTCT
+
9))))/<))).;)()).(.=(,(',(,(,,(75;,(2(2355:((((+)0:65((+62((+(+(((++8585(
@HWI-H225:69:CBJ27ACXX:8:1101:13491:2224 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTCCGTCTTCTTCTTTTAAAAAA
+
DDEABBBD<DD62B;D@@(<=@@AAC377=C;=)77'5'9,5=AAAA?=(((,(,(2(8+44>>A4((:4:+0
@HWI-H225:69:CBJ27ACXX:8:1101:13678:2110 1:Y:0:GGCCTG
GAGCACCCGTCTCTTTTTTTTCTCGCGACAGATCGGAAGAGCATTCGCCTGAACTCCAGTCACTGCCTGAACA
+
1(.)2=(5((0;))))).''-).)(('',''(,:(3(+)((+(((+(&)59226:<<52((((((+(+28(((
@HWI-H225:69:CBJ27ACXX:8:1101:13780:2094 1:Y:0:GGCCTG
CCAGATCGGCAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCCGCTTCCGTCTTCTAAAAA
+
)??3):?;)))0/5>A=>>A.671.==3=A)););7',,3;=55((5(,((((+(&)&0&+&+(+8(+:3+((
@HWI-H225:69:CBJ27ACXX:8:1101:13962:2104 1:Y:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGGAAAAA
+
A@ABB>BBBA;<4>>AAABBA=AAA>BB4>BBA)7AA@@==<;(-;5=;,55((:3&)893:33+5(((4898
@HWI-H225:69:CBJ27ACXX:8:1101:13841:2107 1:Y:0:GGCCTG
TCGATGTCGACTCGAGTCAGATCGGAAGAGAACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGGC
+
;A(=AA===AAAAAAA7)7;>;;69==6;=(5:>=:,9;8383:???;:(8;;;;;;;;33;?(+2(3:?3&)
@HWI-H225:69:CBJ27ACXX:8:1101:13994:2122 1:Y:0:GGCCTG
GTCTCTCTCGGAGCAATGGCTACTGTTTCGTTTTATTTTTCCCTTTTTTGGTGATTTTTGTGCAAGGTGATAT
+
?)1?A777*::))7=>*9<5>9.)))//85(.((.)7).))7)).((.,'',((((,(,)+((+((((++(88
@HWI-H225:69:CBJ27ACXX:8:1101:13781:2199 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTTCTTTAAAAAAA
+
GHIJIJIIJIHGIHJGIEGHIGFAHHJJFIGHHHHFFFDC3(5;@AC??BC3>>8)0(8(4+:>C((+4@CB<
@HWI-H225:69:CBJ27ACXX:8:1101:13983:2244 1:N:0:GGCCTG
ACACGTCTTGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGC
+
JJJIJIJIJDHBGIIJIIIIHIHHGIGIHHHFFBFFCECDBBCBDDDDCCDDDDDDBDDD@BC@>@C?8??9>
@HWI-H225:69:CBJ27ACXX:8:1101:14001:2025 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
=ABBBBBBAAAABBBBBBBA>A8=BBBBBBAA==>A=/==3;;;5==;8:>=??==;;;::3:??(2:??<;3
@HWI-H225:69:CBJ27ACXX:8:1101:14106:2097 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGCCTTCTGCTTGTAAACAA
+
9CB9:BDCD>D6BBD@(8=;=681=C).=A>C=)=A?D@?3,((5>(,,,,,:>8&)&&+(+:(:((+::(+(
@HWI-H225:69:CBJ27ACXX:8:1101:14041:2166 1:N:0:GGCCTG
GCTCTTCCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTAACGGCCTGATCTCGTAT
+
GII:DHGGGDEFC>BFG@AA;BCAE)=AED/?(';??>(;;;<?C8@@@CCAC::((+2<B>>?<>>@C?889
@HWI-H225:69:CBJ27ACXX:8:1101:14189:2184 1:N:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATG
+
HGICGGHBFDGGGGIGAFAGHGG<FFFGE=@F3CGH>CCAE7AC>??BA=>CCCCC:>;A=@B@B@B?C?<?A
@HWI-H225:69:CBJ27ACXX:8:1101:14298:2061 1:Y:0:GGCCTG
ATGTAAACTCGAGCCAGTTCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCTTATGCATTCTTC
+
(.=.))/)7>(('''.(.(.(3(('55((,(5;,288==???:3+:(+8;;=;=((58;3((5((3((++(+(
@HWI-H225:69:CBJ27ACXX:8:1101:14367:2116 1:N:0:GGCCTG
TGTATCGATGTCGACTCGAGTAAGATCGGAATAGAACCCGTCTGGACTCCAATCACGGCCTGAGCTCGTTTGT
+
:?)0:0)8)*/*08<66;(58@)7).)('5(())..6(9'3;95((,:(5>:3((++&0058+((+(0)2(((
@HWI-H225:69:CBJ27ACXX:8:1101:14431:2216 1:N:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCT
+
IIIIIIIIIIIIHIIIIHGIIC;4?CEE?@@BABCCCCCCACCCCCCC@=?>BBC>A>@B8?C9>A<<@<<:(
@HWI-H225:69:CBJ27ACXX:8:1101:14649:2234 1:N:0:GGCCTG
AAGCCAATCTGTCTGTAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCA
+
IIIIIIEGIIIGHEGGIIIIIIFGIHEH=C9;6;;?CDBBB;:?:@ACDA@?@5?1?C8?A9AAC?CCDCA>A
@HWI-H225:69:CBJ27ACXX:8:1101:14863:2141 1:N:0:GGCCTG
CGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAA
+
IIIIFHGHIGIIIIIIFHEHGIEH?EEEEEEECCCCCCCCBBBB@BCCCDCB<<ACCC><B@?A:4::>:(>@
@HWI-H225:69:CBJ27ACXX:8:1101:14766:2161 1:Y:0:GGCCTG
CTGTTCCAACTTGAGACTGGTCAGAAGGGCACACGTCTGAATTTCAGTCATGGCCTGTTCTCTGATGCCCGCT
+
:2).)).)))))9=)()..(.9)./3)('(-5..'.6)9=?3)(.(..6=((((7;(((,,,3(:;02;()05
@HWI-H225:69:CBJ27ACXX:8:1101:14756:2182 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGGATGCCCGCTTCTGCTTGTAAAAAA
+
*:DD<0?8DF=(9?;C;=('8@F@==@;7?AECA)79>3;/1;A@;A9('5'((()&))&+((((((+(+(()
@HWI-H225:69:CBJ27ACXX:8:1101:15128:2019 1:N:0:GGCCTG
CATAGCAGGCGTCCTCCCCATTCAAAGAACTCAGTCTGATAATCTGCCTCCACCAGCCAATGGCAACCAAGAC
+
CDBGC<@<B<D?FGGIA?HHEHD>@4.36?AE>;@?;@C@CC@CCDCCCCBCCB9?BBBCC>38?C2?@?<@C
@HWI-H225:69:CBJ27ACXX:8:1101:15213:2162 1:N:0:GGCCTG
CGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCT
+
HH?D?GH;38BD=@D=F>=B=CC@E1'.=>>?BB86=6,>>>CDA@;(::@',29903:34:12?A9:>500?
@HWI-H225:69:CBJ27ACXX:8:1101:15339:2065 1:N:0:GGCCTG
ACACGTCTTCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGT
+
IJIIJIIJIIFGIJHJJJJJJIIGGHIIJJHHHFFFECEEEDDDBDDDDDDDCDDDDDDDDDBBDD?3@DCBB
@HWI-H225:69:CBJ27ACXX:8:1101:15446:2082 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
;?>?<??>?????<?>>???>?>9;>>>>>;0:):=8?8=<8;7==>;2;7<6;;0568;:(5(8+++8220-
@HWI-H225:69:CBJ27ACXX:8:1101:15561:2154 1:Y:0:GGCCTG
CGATGTCGCCTCGAGTCAGAACGACAGAGGACACGTCTGGACTACAGTCACGGGCTGATCTAGTACGGCGTCT
+
6((=3().((0(07(/7)/<))-''''8.((9..9=(,((((((,(:5;,(,3&)0&+((+((+(((&&&)&)
@HWI-H225:69:CBJ27ACXX:8:1101:15814:2004 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGGTTTCT
+
=A>7.=AAAA=<===;=AA@@@;'6>5;(;;=(;989=??>??=833:;9;=;;78:3829(+((+)&&&)(+
@HWI-H225:69:CBJ27ACXX:8:1101:15753:2015 1:Y:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTACAGTCACCGCCTGATCTCCTATTCCATCTTCTTCTCTTATTTT
+
A*9*9>9..:;1:..=;5)6'.6;5((6((535(5;3-56===8((+++3(+((+(+(+(+((+3((+(((((
@HWI-H225:69:CBJ27ACXX:8:1101:15996:2111 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATATCGTATTCCGTCCTCTTGCTTAAAAAAA
+
DCBDE@;?DDD0B9?BBB85..85A7).)7?;.7@@9?@??;3;(,,(,,(,,5((+&+(((((+((+:><&)
@HWI-H225:69:CBJ27ACXX:8:1101:16189:2055 1:Y:0:GGCCTG
TCAGATGGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTGGTCTCATTTGCCGCTCTATGTCTTCGAATAA
+
)13=<)).<7>=31):=3='<(8518))..8)).;1)8(7=0((..((-(((((,'7;;(3((++2(+26(((
@HWI-H225:69:CBJ27ACXX:8:1101:16144:2126 1:N:0:GGCCTG
ATATTATCTCTTCTTGTCCCGTAAAACCGAAATAAAAATGTCTGCACTCAAAATATAGATGTCTGCACTCTCA
+
D4?*:D9*0*09*09*9?8B@87@FF@-':BD;B)7>(6;BDDB;>@AA?3,>@AB>>>@@;@@@>ABBBB<@
@HWI-H225:69:CBJ27ACXX:8:1101:16075:2207 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTTCAAAAAA
+
JIIGIIGJJJIIIIIGDGII=CCHGDHIJIHHGHHFBDBDD=AACDCBD?CCAC;&528>>4:(4(++44@1.
@HWI-H225:69:CBJ27ACXX:8:1101:16346:2057 1:N:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA
+
IIFFEFEFFFDGF?BFFIF>FCC@D@EFIFFFFEABD4=BBB?B;5>A9<@B?>B>052<:>3:(:(((43(0
@HWI-H225:69:CBJ27ACXX:8:1101:16482:2080 1:Y:0:GGCCTG
CCACGTCTCGATCGGGAGAGCAAAGGCCCACCCTCGAACTCCAGTCAGGGCCTGTATGCCTACGCCGTCTTCT
+
@))1?):)):7=(7'7='--.77))7;(((,',,(,,(,((;,5,,((+(29((+(((,(((+&)000919(+
@HWI-H225:69:CBJ27ACXX:8:1101:16486:2111 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCCTCTGCTTGAAAAAAAAAAAA
+
IIGGICHGC<GHECH2BFIDHGGIHGFDHC@BEHDB?>;ACCB=5>;5@;8;'5:5(+44+(4:>A>30&)&0
@HWI-H225:69:CBJ27ACXX:8:1101:16326:2214 1:N:0:GGCCTG
TTAGTAATAGATCCCACACATAGCATCATAGAGTAGTATGGCAGCCTCACCACACAAAAAAAAAATCGATGTC
+
1CFG<?CCFBHACHIGIIIIGHIGGEGIEIIIHCGEFGGCDHIC;?AACBDEDCD(;;;?BDBBD?(+<B(++
@HWI-H225:69:CBJ27ACXX:8:1101:16328:2235 1:N:0:GGCCTG
GCTCTTCCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTAT
+
HFHJIFCG:@;AF<@AEGHHC<;;@DC:@AB=@6=A?@<?B8(2?8@4:@@C@>>CDC<@@>-2(((+4(+29
@HWI-H225:69:CBJ27ACXX:8:1101:16674:2229 1:Y:0:GGCCTG
TCAGATCGGAAGAGGACACGGCTGAACTCCAGTCACCGCCTGAAACCGCAAGTCCCTCCCCTCCTCGAAGAAG
+
1-1)393(<9(76:(0().559'56:76)6)).=;;1(',3,((((,,&&&&++2/+(+(08-((+)56()((
@HWI-H225:69:CBJ27ACXX:8:1101:16753:2031 1:N:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATATCGTATGCCGTCTTCT
+
??GHGIEGEFBBGH>FH@=@B7;8C;)=B@24.6;5;A;ACCC:@>>C=8@BB(+:(:48+2(3::&551844
@HWI-H225:69:CBJ27ACXX:8:1101:16986:2201 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGAACTCGTATGCCGCCTTCT
+
>EDIIDIIIIIICDIIIEIIIDCADDAA>AA@A???AA>A355>;AAA?>>?><<(:>A>883((:&&0&0((
@HWI-H225:69:CBJ27ACXX:8:1101:16903:2215 1:Y:0:GGCCTG
TCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTCCGTCCTTTTCCTTGA
+
AAABA?ABBB?ABAAA=;=AABAB=BAA<BBAA>AA@?@@)33====9;5995559((5(++)0(((((++3:
@HWI-H225:69:CBJ27ACXX:8:1101:16887:2223 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTCTGAGCCCCTCTGCCTTCCACAAA
+
??><<8:33;=217)7;;?;>?;;;>9)88939=;=;;39;;((,:(,((,2((+(()+)+(((((+(((5(2
@HWI-H225:69:CBJ27ACXX:8:1101:17197:2078 1:Y:0:GGCCTG
GACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGAGCTCGTATGCCGTCTTCTGCTT
+
@>??????=>????><?=;?>?;?????:??;<;?==?>=>>>?<<<75;;((,3&028(+4&&026(2(((8
@HWI-H225:69:CBJ27ACXX:8:1101:17382:2092 1:Y:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAGAAAAAAAAAAT
+
@?<???7>>=<><7>?<>==?>9>?8>86<>?9?>?;??;=???=769;=;<<<<587;2;;<<;<<6:9958
@HWI-H225:69:CBJ27ACXX:8:1101:17364:2223 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTTCTTGGAAAAAA
+
AABBBBBBAAAAB>ABAAB<>BBABBBBBB>>AAA?==@@;==5;;=:5;?35;;&)(233((++(++3:925
@HWI-H225:69:CBJ27ACXX:8:1101:17719:2013 1:Y:0:GGCCTG
GCAGTTCGGCAGATCAAACACCTCGACCACCAGCACGGCGCAATCGCAGCTTCCCAATGAAAATCAAAAACAT
+
<975):)(0(-'().8).<:(()((''',,(,((((,',&&&))(((+&)2(((((++3(((((((++(2&+(
@HWI-H225:69:CBJ27ACXX:8:1101:17631:2014 1:Y:0:GGCCTG
AATAAGCACAGAGCTCCAGGAAGGAGCTAGGTCACCTGTCCTGCATATAGCAGAATCTAAGAGCTTTGTAGCT
+
+2*@@**?=A=ABABBB<>7;AAAB<===>>B;AAAB=.=3>3=7.7););?;>@@@@@@>??=???=>:=??
@HWI-H225:69:CBJ27ACXX:8:1101:17528:2138 1:N:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTTCGATGTCGACTCGATGTCAGATCGGAAGAGCACACGTCTGAA
+
IJGIJIJ?FHIJJJIJJIIGG@@CEGG@CHHGFFEFDEDDCDDDDDDBCDDDCDDDDDDBBCDBCB<2<?@CC
@HWI-H225:69:CBJ27ACXX:8:1101:17829:2241 1:Y:0:GGCCTG
GTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
(@95?8?<8??<<))<);33>))066>>??;58''((,,3:,((,3(&689+8;4:<<<:<<985/:5;;-82
@HWI-H225:69:CBJ27ACXX:8:1101:18020:2028 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCCTCTTCTTGAAAAAAA
+
:<>??????????????<?????<?????????;?@??==<8(5==:;2,(,3:5&0&2+((+(5((+(8<8:
@HWI-H225:69:CBJ27ACXX:8:1101:18402:2147 1:N:0:GGCCTG
CTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGGCGCCTTCTGTTTTG
+
)76?C;FFFC=@FEI@EEAE97?@B>;@@@>@@A5:@:AAB@B=95<89>>:A?+((3(2&&)))(:(+(:+8
@HWI-H225:69:CBJ27ACXX:8:1101:18417:2161 1:N:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTTGAAAAA
+
8CGGGGIJCHHIB;B8CCFD;8=@@@DHIA;A??CEFF@DD@BC3>CAA<<C3(5&)08+(:((::(((++((
@HWI-H225:69:CBJ27ACXX:8:1101:18744:2104 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTTACGGCCTGATCTCGTATGGCGTCTTCTTTTTGAAAAAAT
+
:):4BD9B)BD?B()=BC2;867;4=7?>)7.7)77;=>?>?(>(5((,(5((,)&)98>>3+4>&+(((+)+
@HWI-H225:69:CBJ27ACXX:8:1101:18582:2194 1:N:0:GGCCTG
GCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATAGATG
+
*:B<FFFFI?DF?D?F4C<=B@<B)8;7=C)7C:EAEF>E?>@B>)(.;=35@;>;A>(:998<5@<8+(+((
@HWI-H225:69:CBJ27ACXX:8:1101:18685:2248 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTTACGGCCTGATCTCGTATGGCGTCTTCTGCTTGAAAAAAA
+
FIIIIIFEG)?DDF<FAFEFAFBFEE=@8@AEF;@AEFBDBCAA;;@B;15((5;8=>?>A@@BA4(4:>29<
@HWI-H225:69:CBJ27ACXX:8:1101:18998:2139 1:N:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTG
+
FICFFIIFGIFFFIIAFIBCFEFAE@E?EFF@DBBDDCC>ACBBBBBBBB35>A?+<33:>9-9<B@A3(:>A
@HWI-H225:69:CBJ27ACXX:8:1101:18787:2187 1:N:0:GGCCTG
TAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCGCGTATGGCGTATTCTGCTTGTAAA
+
*:<?F>DC<DBDEGFDG3CBFEADHIIIIAEAACCA;?BECCBBB=3:(58'08++8555(9(4(++4(((+:
@HWI-H225:69:CBJ27ACXX:8:1101:19017:2127 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGCAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCCTCT
+
:)==3(0(7-(7)<=8;>???'--3=>3=??'.69;((5;<><<=<;=;599-28522:7(+(+(4&&)&+((
@HWI-H225:69:CBJ27ACXX:8:1101:19171:2146 1:Y:0:GGCCTG
AACCAACAGTAAGTAGATTTGAACCCTTTTTCCTGAGTGTCTGAAGATAGATACCGCTAACTTACATATAATC
+
6=(@@3(((0):))))0)))).).0(())/).)).).>).)).6)8:.6)).).)'''''-5((,5,2578((
@HWI-H225:69:CBJ27ACXX:8:1101:19459:2004 1:Y:0:GGCCTG
AGAGACTGGCCCTCCCGGCTACTCAAAGCACAGTAAAGACACTAAACATGTGCTCTGTCTGCTCTGAGCCTGG
+
(()2)=8617;::>1<(--5()::>)6.)80=:9)==;?;(.6.3;-(-5-:6(,35,5((,2;:(8:(5289
@HWI-H225:69:CBJ27ACXX:8:1101:19475:2025 1:Y:0:GGCCTG
GCAAAGTCAGTAGCAGCAGCAGCAAAAAAAAAAACGATATCGACTCGAGTCAGATCGGAAGAGCACACGTCTG
+
=33=:>)79<3:))9=7>1(<;;>>??;=8;5<&5-06(&4888<326&+6:/62+28665755;<<<9/889
@HWI-H225:69:CBJ27ACXX:8:1101:19457:2026 1:Y:0:GGCCTG
GCAAAGTCAGCAGCAGCAGCAGCAAAAAAAAAATCGAGGCTGACTAGCGCTAGATCGGAAGAGCACACGGCCG
+
-79<77=9)<1)(911:6>9(663=)):',',02:(8(&&((+85(+(0&+)5/4:<52()(((((+(0&&&&
@HWI-H225:69:CBJ27ACXX:8:1101:19277:2183 1:Y:0:GGCCTG
CATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGCCCT
+
)8==:3=<?=?<?/)<=?<>?;36=??07><;?59==;>=>=<>?05;<=<<<:59;::(2(++((+5&&)&)
@HWI-H225:69:CBJ27ACXX:8:1101:19369:2234 1:N:0:GGCCTG
TTAGTAATAGATCCCACACATAGCATCATAGAGTAGTATGGCAGCCTCACCACACAAAAAAAAATCGATGTCG
+
EFCEE?E:C*C<DEDE;DDC3DDE@DDEEE:CB//=@@C=@DEIDCDCCDDDDD@@@>;???9>(:2284<32
@HWI-H225:69:CBJ27ACXX:8:1101:19523:2043 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTACGCCCTCCTCC
+
BB=?;=A(;<(;=AA)>AA@@==<>>3;;>==;===39:=??=3:??3&09;;<++3(32(2(&&0&&+((((
@HWI-H225:69:CBJ27ACXX:8:1101:19580:2097 1:N:0:GGCCTG
TTAGTAATAGATCCCACACATAGCATCATAGAGTAGTATGGCAGCCTCACCACACAAAAAAAAAAATCGATGT
+
?FFCH?D9C9C?DFHIGEIG<D9BFEGHEGAHH)8@=@CDDEGA?CHEEEEDCCCBBBBBBBBBBB1((2(4<
@HWI-H225:69:CBJ27ACXX:8:1101:19525:2197 1:Y:0:GGCCTG
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCCTCTTCTCCTTGCAAAAAA
+
0@DDDCEDIIIDDBCC@AAD@CCDDCAA@A>A@AAA@@>????>>A><85(9>A((+++4:(((+((+4>3<5
@HWI-H225:69:CBJ27ACXX:8:1101:20101:2121 1:Y:0:GGCCTG
AAAGGGCTGTAGGCAATTGGTTTTGGATTCCGTTAAACCAACATTTATGTGGTTAGACATGGTCATTATGGAT
+
)2(.)-(=(())0;8().)).(0(.'.))))).)--6))(((,9=)8);9((63;;=77=(,;:50(72((,2
@HWI-H225:69:CBJ27ACXX:8:1101:20093:2171 1:N:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGGAAGGCGGCTTCTGCTTG
+
8:?0?DA:B@G@B08@D;ACA382=.;(.=;?@.7.;?C6(.(9>8?B@<5:@(9(&()((&&&&)3+:(+4(
@HWI-H225:69:CBJ27ACXX:8:1101:20110:2180 1:Y:0:GGCCTG
CCTCGAGACAGATAGGGGGAGAGCACATCTGAACTCCAGACACAGCCTGAGCTCGTATGCCCCGTTCTTCTTT
+
*1*:)1?))00:***0(('-;'(.=A)..77)(((.6>;(,,;(,((,,(((58&)++(++((&)+(2(((++
@HWI-H225:69:CBJ27ACXX:8:1101:20464:2062 1:Y:0:GGCCTG
GCTTTAAAAGATCCAAGATACAAAATCACAGACTAGAACGGGAGCCTAAACAGACATAAATAAATCAATAAAG
+
0()).00)0)(0/8)).8)/))))(.;3))8)(.3=8=8'''3,,,(,,3(,((+((+(((+3((5((3(++2
@HWI-H225:69:CBJ27ACXX:8:1101:20441:2077 1:Y:0:GGCCTG
GCTCTTCCGATGCCGGTTAGAGGAAGATCGGAAGCGCAGAAGTCTCGATGTTGACGTGAATCAAATAGGGAAG
+
0(03(.)>(593(.''--'..;((..6((6,;(,(32'&&((+2(+6/(2((4((&((+(++8+2(8<((5++
@HWI-H225:69:CBJ27ACXX:8:1101:20345:2097 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACAAGTCTGAATTACAGTCACGGCCTGCAATCGTATGCCGTCTTCTGCTTGAAAAAAA
+
?)3):.):5;=6<=9<10(..86=<;)8)).;68>65=??8?3(.(66((-91;''307(+6(32(+3:<<58
@HWI-H225:69:CBJ27ACXX:8:1101:20357:2111 1:Y:0:GGCCTG
CGGACGAGCACACGTCCGAACTCCACTCCCGGCCTCCTCTCGTATTCCGCCTTCTGCTTCTAACTTACACACC
+
2.0<(((7;-<=>;(6(''59((.((((,9,82;/((+8660((++4:(&+006:(86;((++(+((+285)8
@HWI-H225:69:CBJ27ACXX:8:1101:20337:2113 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGGCTTCTGCTTGAAAAAAA
+
2939986=;99;>>1=(7.7.;;=>8)..<3>863;9=<<;<>1-(6;(3:;,;<&5:6<<<::<<7::5255
@HWI-H225:69:CBJ27ACXX:8:1101:20424:2249 1:N:0:GGCCTG
CCTGATTGAAGATGAGTGGGAACCTTCCCAATAAACACGCTTTGGATATAAAAAAAAAATCGATGTAGACTAG
+
9@ACFBG@<DGF>GF>9?AE@;6?8CDFFII3@@E77(''5;;BD>;@@D@;;@@B9>9((+22>2(+>>>(3
@HWI-H225:69:CBJ27ACXX:8:1101:20532:2001 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCCCTGCCCGCTCCCCCCCCCCAAACA
+
)1:.8))<=5;;>3<.<>(7<=<5;6;=)).:;<;>/==;3/4-(-:37;522,(')&&)2))5559)&+(++
@HWI-H225:69:CBJ27ACXX:8:1101:20541:2114 1:Y:0:GGCCTG
ATATTCTCGCTTCTTCTCCCCGGAAACCGAAACTAAAATGGCCCCACTCAAGATACAGAACTCCTCACTACCA
+
)*1*1**1)0:)8*909***.-'-'').-,'',((--;(-(,((,(',;(+(((((+5:(+(4(58(((+(+(
@HWI-H225:69:CBJ27ACXX:8:1101:20746:2228 1:Y:0:GGCCTG
CATAGCAGGCGTCCTCCCCCTTCAAAGAACTCAGTCTGATAATCTGCCTCCACCAGCCAATGGCAACCAAGAC
+
BBBBBBBBAAA=AAABBAA/=AAA>>AA(;?@;);>@@@B@;>?B;????====:9==23888;?3;==(2(9
@HWI-H225:69:CBJ27ACXX:8:1101:20961:2063 1:Y:0:GGCCTG
CTCGAGTCAGATCGGAAGAGAATACGTCTGAACTCCAGTCACGGCCTGATCTCCTACGCTCCCTTTCGTTTCC
+
=5=9>;<?<<<?:>?(-'<7)6).8::(:(;?;?=;7(:09;'5;',2(58((((((&&(&&+283+((+2((
@HWI-H225:69:CBJ27ACXX:8:1101:21102:2108 1:Y:0:GGCCTG
TCAGATCGGAAGTGGACACGTCTGCAATCCACTTACTGCCTGAACTCGTTTTACGGCTTTTACTTTCTATAAA
+
)00=<>9(0-(<)..;).(-((.().))).)).))))..((6(((-(,,(,((,(',&&+3(+(5(5+++(((
@HWI-H225:69:CBJ27ACXX:8:1101:1197:2411 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTTAAAAAAA
+
FHGGHGIIIEIIGGGGGGHGCCC;@CEHGIHHA)=@BD8>B@=;@;@A1<,,(:859<<3((+(4((+:>>55
@HWI-H225:69:CBJ27ACXX:8:1101:1118:2459 1:Y:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATG
+
??????>=>??<???>?????????>?>?>??;???@??;?>;????;;7=>?>>7::7:<<<9:9:/++8:/
@HWI-H225:69:CBJ27ACXX:8:1101:1717:2298 1:Y:0:GGCCTG
TGTATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTAATC
+
??>?<?517=>?>>8??1=?)6::>?;9;;?8;9=>;><;9;<5077=<;<<=<=::-850:3+(58/+(((+
@HWI-H225:69:CBJ27ACXX:8:1101:1692:2417 1:Y:0:GGCCTG
AGTCGACTCGAGTCAGCTCGGACGAACACACGTCCGAACACCAGTACCGGCCTGATCTCGTATTTCGTCTTCT
+
00:0=)7((/7').88).)'''''3'''(,;3;=(&)87&(((++(((&&)&0((433:889=(((+2+8(3+
@HWI-H225:69:CBJ27ACXX:8:1101:1939:2487 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
FHEGIJJJIIJJIIIJIIIIFGFHEIIGFHGCEEFF6ADB6?C@CDDDBBDC@A90912:4(:@@(+4>@ABD
@HWI-H225:69:CBJ27ACXX:8:1101:2149:2316 1:Y:0:GGCCTG
TCAGAAAGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGCATGCCGTCTTCTCCTTCGAAAAAA
+
1:?<=***:?>2>))9(>=69=;-=A37;A3=A).7'99=61;-;>?;(,8((,'0-(+((((+(((++2025
@HWI-H225:69:CBJ27ACXX:8:1101:2032:2350 1:N:0:GGCCTG
GCTCTCCCGATCCGACCCGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGTCCTCGTATG
+
CDADD<DDDD0A;C@;-;/4'',((=(-(,(5;925:=9<0++32:>>AA>A(::+:090;-2(((+:)(((+
@HWI-H225:69:CBJ27ACXX:8:1101:2155:2352 1:Y:0:GGCCTG
TTAGCCGAATCTTGAAAAGCCCAGAGATCCAAAGAGCCCTTCGAGCACCACGCACGAAGATCCATCGCAGAGT
+
=8).0:36=?=>>?6==>?<;?>?;><?????1;=>>==;;>??8;3;<2;;3:-595-&(382:<6660&+(
@HWI-H225:69:CBJ27ACXX:8:1101:2283:2456 1:Y:0:GGCCTG
TGTCGACCCGAGTCAGATCGGAAGAGCACACGTTTGAACTCCAGTAACCGCCTGAACTCGGATGACGCCTTCC
+
(/0077='5'545A)77;76==;'52;;(55'((,,3:83(8:83((++5;;5++(+(+((&2-(5(&&()+(
@HWI-H225:69:CBJ27ACXX:8:1101:2750:2262 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAGAAAAAAAAAAT
+
IIIIIIIIIIIIIDIDEIIIIIEIIIIIIIIIIIIEEIIIDDDDDDDDDD@AAAA????AAAAAAAA?>???>
@HWI-H225:69:CBJ27ACXX:8:1101:2595:2427 1:N:0:GGCCTG
GTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCGATGGCCCCCCCTC
+
009B1;(;(.;)=4=;)=295;9@A<A????99<5:5:>AA3::>>A;-05>73(4(++&&+0&((()0&0&&
@HWI-H225:69:CBJ27ACXX:8:1101:2972:2325 1:Y:0:GGCCTG
CCCGGTTCTAGCAAAAAGAAATTGATTGACTGAGAATCTGATAAAGCACGAAAAGAGTTGTCCCAATAAAAAA
+
8?3(-<6=3)3>??7)('(..))/))8>)....3)8=...).)((6==(''3',(((,((7((5(((+86(+5
@HWI-H225:69:CBJ27ACXX:8:1101:2977:2376 1:N:0:GGCCTG
CACCGCTGCCACCCTGCTACTGCAGGGCGAGGAAGAAGGGGGAGAAGATTGATCAAACTGAATGAAACACACA
+
GGIIIIIIIIDGHI().=@4.=@=;CH1=49?'5;(5,5=B&55&8(++::((+>AC(4:3@4::AC(9(208
@HWI-H225:69:CBJ27ACXX:8:1101:2978:2462 1:Y:0:GGCCTG
TGTCGACTCGAGCCAGTTCGGAAGAGCAAACGTCTGAACTCCAGTCACGGCCTGATCTCGTACGCCGTCTTGT
+
(*.)9('-5;5>''.(...;9<(,;5(((,58;:(,8:88;;(+3(4(&)00&2(++(+2++(09;;&5;9((
@HWI-H225:69:CBJ27ACXX:8:1101:2902:2473 1:Y:0:GGCCTG
GCAGATCGGAAGAGCATACGTCTGAACTCCAGCTACGGCCTGAACCCGGACGCCGCCTTTCGCCCGGAAAACA
+
(1(9=81(<=9?7:8=)6=>6;)51=08:?):8))65=/;36((,('2'&&&))&)0&)3(28&&&)&)02(+
@HWI-H225:69:CBJ27ACXX:8:1101:3002:2288 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAATCACCGCCCGATCTCGCATGCCCGCTTGCGCCTTGAAAACT
+
1:*?0*05)==(((/)(9=4.=)-7>)==A>)).;;)('3,,,',(5((&(((+())0&(()&&&&((++83(
@HWI-H225:69:CBJ27ACXX:8:1101:3088:2377 1:N:0:GGCCTG
GATCGGAAGAGCACACGTCTCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG
+
ADGIIIIIIIIIFICFFEFFIECEFIIIEFFDDDCACCCBBBBBBBBBBBB??BB??BBBBBBABBBBBBB@<
@HWI-H225:69:CBJ27ACXX:8:1101:3301:2269 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTCCGTCTTCT
+
???>???92;??>??;8?<?8==????????<=<?<>?>??>?;==<07;7:98;<<<4968;(46(368:6+
@HWI-H225:69:CBJ27ACXX:8:1101:3439:2270 1:Y:0:GGCCTG
TCAGATGGGAAGAACACAAAACTGAACTCCAGACACGCGCTCTTCTCTGTTGCAGTGTTTTGATTTCAAAAAA
+
)1.<.)09.))(0)))..(((..93:).6)<:)(<=''',9'(,(,((,,7;(((+(+8+((+4((+4:/+(&
@HWI-H225:69:CBJ27ACXX:8:1101:3294:2287 1:Y:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCTGATCTCGTATGCCGTCTTCTGGTTGCTAAAAAAATAC
+
ABB4;A<>=A>B=AA8=AABBBBBBBBBBAA/5(59>;;>5>;(((,(:;',(5(3+((+(((+5(++))(+(
@HWI-H225:69:CBJ27ACXX:8:1101:3468:2299 1:Y:0:GGCCTG
GTTCTTCCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTAT
+
A)0909==AA<4==.6;AAAAA=7>3;;;='55/;=;9(9:====9?;:;??8(8(+85&-9999??83;;08
@HWI-H225:69:CBJ27ACXX:8:1101:3425:2410 1:Y:0:GGCCTG
TCGAGTCAGATCGGAAGAGCACACGTCTGAACTTCAGTCACGGCCTGATCTCGTAATCTCTCTTCTTCTTTGA
+
11?=6=)?A?A>AAAA<B(.)(9.5.6)(>BAA;A>=7).>',3'95;59,;(,558((+(((+++(+(+((+
@HWI-H225:69:CBJ27ACXX:8:1101:3601:2268 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAAATCGA
+
?GEABACB?DHHIHIBDFHECHFBFAGGE@DAEHHEGD=@ECE;?BEDEECCCBBCCCCCCBBBBB0<>?C??
@HWI-H225:69:CBJ27ACXX:8:1101:3637:2316 1:Y:0:GGCCTG
TTAGTAATAGATCCCACACATAGCATCATAGAGTAGTATGGCAGCCTCACCACACAAAAAAAAAATCGATGTC
+
EEEEE?:??*:?CDDEDI>DEC4?DDECCEII:=CA@CA;C=CCCD>AD=AA@=@@?AA??:>???>818:>>
@HWI-H225:69:CBJ27ACXX:8:1101:3701:2344 1:Y:0:GGCCTG
GGAAGAGCACACGACTCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGCTGAACTCCAGTCACCGCCCCA
+
>;3=(.6>?7=?/(79=-())./:.;/=/65????>1=33(7;083;;;;285&8:</(:66:<5:(289&&+
@HWI-H225:69:CBJ27ACXX:8:1101:3880:2418 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTTAAAAAAA
+
IIEIIIIIIIIIDIDIIDDDCBACDIDIIIEIDDDDDDD@@@>;>DA?58>((:8')+84((44>((++:>>;
@HWI-H225:69:CBJ27ACXX:8:1101:3990:2462 1:N:0:GGCCTG
CTCTTCCGATGTCGACTCGAGGCAGATCGGAAGAGACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATAC
+
AD6D<48?@B@C;==ACDDD6,55;=3;(9>??99(5<?>8><?AA>::>A:A:>A-9>>97(434><8<((+
@HWI-H225:69:CBJ27ACXX:8:1101:3909:2482 1:Y:0:GGCCTG
TCAAACGCGCTCTCCAAATACTGTCCTCCGCTGCTCGCTCGACTGGGATCGTTCTGAGAAAAAAAATTTATGT
+
09))))('7-9'(.))))).8));=(9969;5'8((('82)5&()52(((&+((+42(56(++0-0(((+(++
@HWI-H225:69:CBJ27ACXX:8:1101:3918:2497 1:Y:0:GGCCTG
TCGGTAGAGCGCACGTCAGAACTCCAGTCACTGCTGGTTCGCATGGAAGCTTTTTTCTATTGAAAATAGAAGA
+
2=-(((:):)(((('(((':)))8;)))).;38===;(:(''',,(,,((((3,:5(+((((((++((+5(((
@HWI-H225:69:CBJ27ACXX:8:1101:4228:2329 1:N:0:GGCCTG
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
<):?=B;;@BDA/9BDCCDA==@7AC;C>CC7=.;?>9<>?;,(,5:5,5(9:>&)58<>>4+4:((4:><.&
@HWI-H225:69:CBJ27ACXX:8:1101:4164:2455 1:Y:0:GGCCTG
TAGATGACGAATCGAGGCAGAACGGAAGAGTGAACTCCTGAACTTCAGTCACGCCCTTGTCCCGGATTCTGCC
+
.).).;))(('.,((;'',5;((0&8(0+((((3;((33;:(((((38(+3(&(+0&((+:(:&&&&&,((((
@HWI-H225:69:CBJ27ACXX:8:1101:4047:2499 1:Y:0:GGCCTG
TCATTGATTTGGTTCAGATACTATATTCTACAGCTTGATCTAATGTAAGCTCTCTGGCAAGAAAACCATCTTT
+
--((/.))))).(.7>>97)).))((..(.((53(5;(((,((,,,(59((,((++(((+((+((+(+88((+
@HWI-H225:69:CBJ27ACXX:8:1101:4462:2323 1:Y:0:GGCCTG
GCTCAGGTGATTATCCTGAACCATCCAGGCCAAATCAGTGCTGGCTACGCTCCTGTTCTGGATTGTCACACAG
+
.:9<@<?1=>??=?=?:3=???>?:<?8>>?@@>?;??:=??=<??@=8=<==??>?>>=:<=<8<55;;;;<
@HWI-H225:69:CBJ27ACXX:8:1101:4486:2370 1:Y:0:GGCCTG
GCAGAGCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGCCCTCTGCTTGGAAAACA
+
9((9(()7=;=<5:>9:7(5.=:;16=8=?9(63==;3;;;7582;(((+((+80&&&06+::5(((+((((2
@HWI-H225:69:CBJ27ACXX:8:1101:4321:2466 1:Y:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCGACTCCCGCCTCTTCCTT
+
;A)1:AA3=A?====AAAA2(==AA>:AAA=>@@@@@9>>@@>33;=75955,;95&&&&32&&)5&(+((((
@HWI-H225:69:CBJ27ACXX:8:1101:4542:2301 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGAGTTTGTCTTGCGTCTTCT
+
0*)/'(--'-.').=).).(,(('(,(;;;;=??<8(::5>((++:(+&)090((++4(:<(+(((&&)9<34
@HWI-H225:69:CBJ27ACXX:8:1101:4537:2364 1:Y:0:GGCCTG
GCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
AA;AAABBA<A=>@@@@@@@A=;=;;>?9>?>>(5;;===;=8+:;?=9;=;??))589?:;=7;3(+5=9.5
@HWI-H225:69:CBJ27ACXX:8:1101:4622:2388 1:Y:0:GGCCTG
CCTGTCCTTGTTATCCGCTTCCACATGATGAGAAAGAAAGACTCAGATAAAAAGTGACATTTCACCTTTCTAG
+
.))..87).)2)))2)(-((0))09<>;).<)).).)6))6)3>))))).=))5)(((.==(6:(((((-(78
@HWI-H225:69:CBJ27ACXX:8:1101:4728:2389 1:Y:0:GGCCTG
TTGATTTTTTATATTTTCTTATTTTTTAGCTCAAAAAAAAAATCTATGTCAAATGGCGTCATATAAAAAGATG
+
&()(((6(6-8((5(((((6:(((6(&(8(+3:<<(8&5::56:(:(((+3((((52&0&&((5+((((&+((
@HWI-H225:69:CBJ27ACXX:8:1101:4630:2405 1:Y:0:GGCCTG
CCAGTCCTGGATATCCCAACACACATGAACAGAAAGCAAGATTCAGATTAAAAGTCATATCTCATGATGCTAG
+
=>?@=@?>?<9>=?=???????????????@@@?@@??;?;>??>=>@????>?8=>>>????>==>:>>===
@HWI-H225:69:CBJ27ACXX:8:1101:4789:2343 1:Y:0:GGCCTG
TCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGC
+
290B:B66=;ADDCDEIIADDA@A>@DA@@=???AAAA>>A>A>>A?>>>???AADA???>>AA>95>>3:(4
@HWI-H225:69:CBJ27ACXX:8:1101:4916:2424 1:Y:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAAATCGA
+
?@?:?????<?>???>???=??>??>????@@??;?@@@@@@@@@?<?????=<???;><;<<<:5:::;<<5
@HWI-H225:69:CBJ27ACXX:8:1101:4764:2478 1:Y:0:GGCCTG
ATTATTTTTCCTGTTATGATCCTGGCCCTGGTAGCAGTCAGAAAAGGTATGGATTACCTCTTCTCCCAGCACG
+
*?BBBBBBBBBBBBBBABBBBBBBBBBBBBBBB>BBBBBBBBBAAAA@@B@@@@@@@@@????????=?==96
@HWI-H225:69:CBJ27ACXX:8:1101:5105:2303 1:Y:0:GGCCTG
CCTGATTGAAGATGAGTGGGAACCTTCCCAATAAACACGTTTTGGATATATAAAAAAAAAATCGATGTCGACT
+
DIEIIIIIIIIIIAEI?BDDDCDIIIDEIIEIIEECEDDCDDDD?DADBEEEEDAA???>?>A7>>>?A??>>
@HWI-H225:69:CBJ27ACXX:8:1101:5159:2389 1:Y:0:GGCCTG
TGTAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGACCTCGTAAG
+
AAB>A?AA2=/0?(=:<<;=4=.7777===@@@@;(;5;5/,(98;9=3;>8:38=?-)05&(3(3:=02;((
@HWI-H225:69:CBJ27ACXX:8:1101:5196:2432 1:Y:0:GGCCTG
ACAATGGTCATATTCAGATACTACATTCTTCAGCTTGATTCGCTCGAAGCTTTCTGGAACAAAAAAATCTATA
+
(8(0)077..))9..))7)78.)).)/.))).))8).(..)('5(',79;><===5((,((8(+(086(((+(
@HWI-H225:69:CBJ27ACXX:8:1101:5370:2353 1:Y:0:GGCCTG
CCTGATTGAAGATGAGGGGGAACCTTCCCAATAAACACGTTTTGGATATAAAAAAAAAATCGATGTCGACTCG
+
>?<96?><6=)<1=<1)0=<8;/=7??36>?90;=;17;.(,90'(,8==6;;:985:95685828(+2&(5(
@HWI-H225:69:CBJ27ACXX:8:1101:5290:2363 1:Y:0:GGCCTG
ATGTCGACTCGAGTCAGATCGGAAGAGCACACGGCTGAACTCCAGTCACGGCCTGATCTCGGATGCCGTCCTC
+
BBBBBBBAAAAAA>@>>@@@@@@=====?===='57;;??=?=:;????======9(3(8;(&+&28)&)&2(
@HWI-H225:69:CBJ27ACXX:8:1101:5402:2390 1:Y:0:GGCCTG
TCAGATCGGAAGAGCCTACGTCTGCACTCCAGTCACGGCCTGATCTCGGATGCCGTCTTCTGCTTGAACAAAT
+
14*1**:)4?)8)0)/**/'(.=())8=AAB>BA;=6==6<=(-5((5(35',9=&0(8;8=((+3((((3((
@HWI-H225:69:CBJ27ACXX:8:1101:5398:2407 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
DDIIIEIIIIIIIIDIIIIAACCDCEEIIDDDDDDDDDA@??>>ADA???A=AA;59??>AAAAA>1:>A<;9
@HWI-H225:69:CBJ27ACXX:8:1101:5397:2477 1:N:0:GGCCTG
TCAATGGAGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCTATA
+
:C?D<***0*0098BDEFF*).//8==.)))).7.7@E=((77@;);?>B>6@AA3;;@@99;<-552((++(
@HWI-H225:69:CBJ27ACXX:8:1101:5622:2266 1:Y:0:GGCCTG
GTCGCCTCTTGTCCATTCTTAAAAGCAAACCCCGTGATGACTGCCCTAGTCAGATCGTAATGACCCACGCCAA
+
)0)9(('-))..8.))).)).<.8)()))((,'''',,,(,(75(,(((++/2((62(((+(+(++(+))6&)
@HWI-H225:69:CBJ27ACXX:8:1101:5533:2356 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTCGAGTCAGATCGGAAGAGCACACGTCTCGAGTCAGATCGGAAGAGCACACGTCTG
+
IIIIIIIIIIIIIIIIIGGIGIIHGIEIIIIGHHFFFFFCE?@BCCCCCCCCCCCC<BB@BB?CCBCBB?BCB
@HWI-H225:69:CBJ27ACXX:8:1101:5561:2428 1:Y:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATG
+
BBBBBBB=ABABBBBBBBBBBBBBBBBBBBBBBBBBBBBAA>A@@@>@@:@@@AB>>=??>=A====<?;=;(
@HWI-H225:69:CBJ27ACXX:8:1101:5829:2260 1:Y:0:GGCCTG
TCAGATCGGAAAAGCACACGCCTGAACTCCAGTCACGGCCTGATCTCGGCTTCCCCCCTCTTGTCTACGACAA
+
..>1=)<>6(<(:3(<>?7='7(6;3;1=>7>.6=>8?;5;=>(:((,(((',,,()))0(5(+((((()&)&
@HWI-H225:69:CBJ27ACXX:8:1101:5757:2396 1:Y:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCCTGAAAAAA
+
?=>A?<AAAA<AABB9>2>>>BBAABBBABAA>AAA@==@=6=355;?2=(9(,50)0(283(+(4+(((+(0
@HWI-H225:69:CBJ27ACXX:8:1101:5901:2446 1:N:0:GGCCTG
AATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTCCG
+
II9@(=?>EHDEE;>B@BCCC>A@C?BBB?8??C8?B@8?CBCCCCCCA@@C@C>59@083+:@@(+23(+:3
@HWI-H225:69:CBJ27ACXX:8:1101:6086:2278 1:Y:0:GGCCTG
TGAAAACTCGAGTCAGATCGGAAGAGCCCACGTCTGAACTCCAGTCACGGCCTGACCTCGTATGCCGTCTTCT
+
..)))/69>(.-.;>8=;)=;59'(,6(((732;;<<=:::::6:/6;0555&(((++:<<3;((5&0&(2(+
@HWI-H225:69:CBJ27ACXX:8:1101:6019:2370 1:N:0:GGCCTG
TCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCCGATCTCGTATGCCGTCTTCTGC
+
E;0?DDHHDG*9B@@?)BA;C;86=@;C;:6?BBBD66>CCCCCC>;B@B')&))+:2+(3<>A0)0824+((
@HWI-H225:69:CBJ27ACXX:8:1101:6228:2377 1:N:0:GGCCTG
ATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCCTCTTC
+
GH@DFHIIIGEEB?CB@DDEDDA=;?ACDBDD@,<?BCDCDDDDCDCDDDDDDBB<:@CCA02>(4:(489>:
@HWI-H225:69:CBJ27ACXX:8:1101:6079:2422 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAAATCGA
+
AE@FGF=FFEFIIF@BDFEEEI@FFCGC@3@FF6@@>@DEEFFFA:??BDC@C@BBBBBABBBBBB9>BBB<@
@HWI-H225:69:CBJ27ACXX:8:1101:6413:2335 1:N:0:GGCCTG
GCTCTTCCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTAT
+
HIIIIHIIGIIIGEGIIFHHFF9;AEECCBCCCCBCCCBCBCBCCCCCCCCCCCCCCCBCB>B?C>:CCBBBC
@HWI-H225:69:CBJ27ACXX:8:1101:6410:2392 1:N:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
HCHGGGGHIGHGGAGHDFGHIIFHIGGIHIIGEEC9@=BB3?@?B2<BBBB(<CCCCCBBBBBBBCCBCBB2<
@HWI-H225:69:CBJ27ACXX:8:1101:6310:2423 1:Y:0:GGCCTG
TCTGATCGGAAGAGAACACGTCGGAACTCAAGTCACGCCCTGAGCTCATATGCAGTAATTCGATTTACAAAAA
+
):0;:>368=6-.73))).-..'-593(6(:(--9;53(,330(5(((+2(+2((+((++(+(++6((4((++
@HWI-H225:69:CBJ27ACXX:8:1101:6344:2448 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAACACAAA
+
HGGIIIBHIFIIIFHGHIIIG@CHGIHCHFHHHECCCAC3;A@5?C<:>=8?<@@:4:(:+++4>@<&((+((
@HWI-H225:69:CBJ27ACXX:8:1101:6440:2486 1:Y:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATATCGTATGACGCCTTCCACCTGCAAAAAAAGCAT
+
4?=)5?4=B=>;>AA>BBAABABBAA7>BA3//96<(;((-;=(((,(5('3()(((+(2(((3;9<5)&(3(
@HWI-H225:69:CBJ27ACXX:8:1101:6252:2494 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
ABBBBBBBAAAABBBBBB>AABAABBBBBBBBBBAAAA@=9=@;?;?=;;99;>='3(8::(3(+(+(5:9=;
@HWI-H225:69:CBJ27ACXX:8:1101:6589:2273 1:Y:0:GGCCTG
ACAGATCGGAAGAGCACACGTCAGAACTCCAGTCACGGCCTGATCTCCAAACCCCTCATCCTCCTTTATACAA
+
(<3=7)93(9639())(93'(6(5=;7=>97));:=9/'9'(4(73=(,:(((,'()(+((+((+++((((+(
@HWI-H225:69:CBJ27ACXX:8:1101:6677:2312 1:Y:0:GGCCTG
CCCTAGTCAGGTCGAAAAAGCACTCGGCTAAACTGCAGACACAGCCACTTAACCAGTCTGCGTCTTGGTGCGT
+
2)().:)1.1)2=(((((((().))((((()<)>))):)(())(6((((-4(9-5,:73;0',,82((+((&+
@HWI-H225:69:CBJ27ACXX:8:1101:6577:2382 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCCTCTGCCTGAAAAAAA
+
<FGIIIJIIBFFHGEHCGGHEE@DGH@;CD>EHCHD4=>A8=55;>@5(8>95>809&+4((((4((((4?@@
@HWI-H225:69:CBJ27ACXX:8:1101:6618:2397 1:Y:0:GGCCTG
TTGAGAATCGAACAAAAACGGAAGAGCCAACGTCTTAAGTCCAGAAGCGGCCTGAAATGGAAATCGCTTTATT
+
()(/;().).3).5)((.9'93,,(,7((,78(,8((5((+(4(28(3&&0:68<4++((((2(6((+8(+32
@HWI-H225:69:CBJ27ACXX:8:1101:6563:2402 1:Y:0:GGCCTG
TTGCGAATCTGTTTCTTACAAAAAAAAAGATGTCTACTCTCTTGAAATCGTTTGAGAACGAATTTCAACACAA
+
1)..-5((3))0..).)))0<>.(-''',,6=9((.((7(-((((((((,,2((8((5(&&+85(+8:(+8(+
@HWI-H225:69:CBJ27ACXX:8:1101:6503:2496 1:N:0:GGCCTG
GATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTT
+
HGIHGIIIIGIIIIHHIIIIIHHFDDFDCECEEBBCCCCCCCCCCC@CCC>BCBBBC:>CCB?BC?CCB>B<@
@HWI-H225:69:CBJ27ACXX:8:1101:6777:2306 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCCTCTTCT
+
BBBBBBAAAABA>BBBB>>AAAAAAAAA>@@@;=>@@??????????A====;9;:=??2(++(88(+(((((
@HWI-H225:69:CBJ27ACXX:8:1101:6796:2391 1:N:0:GGCCTG
GCTCTTCCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTAAACTCCAGTCACGGCCTGATCTCGTAT
+
@GHGGIIIIIIIIIIIIIFHFE@BDECCCCCBBBB@CCBCBBABCBCCCCCCCCCCCCBBBBBBA:>@C<(8?
@HWI-H225:69:CBJ27ACXX:8:1101:6917:2414 1:N:0:GGCCTG
CACACTTGAGTAGTGGGATAAGTCACTGTTTTCTTTACAAAACAAAACAGTCCTGTTGGGAATGATTGCTGTT
+
AAH@FCHC><:BD:3BA??DGH>48CFH4B@G@;@FECGEACEEH;?BEE>CCCC>CC@B;>?C@CDCCA@>@
@HWI-H225:69:CBJ27ACXX:8:1101:6823:2479 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCTATTCCTTCTTCTTCTTTTAAAAAA
+
1?3A<<ABAAA=BBB>A9AABA=AAAA=AA>;?)7;6'99===399,(,(:(,5((+3333(8(5((++3(+5
@HWI-H225:69:CBJ27ACXX:8:1101:7201:2337 1:N:0:GGCCTG
CGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCTATGGCGCTCTCTGACTGCTA
+
)8@:<=@<8@C(;CEFE;==6;6<(;@@BAA5;(55;>@BBB'09B?+4(4+4+((((&&&&++((((++(++
@HWI-H225:69:CBJ27ACXX:8:1101:7134:2361 1:Y:0:GGCCTG
TCGGAATAGGAAACGTCTGAACTCCAGTCACGGCCAGAGCCCCTTTTCCCACTTCGTCTTTTAAAAAAAACAA
+
??)00?)*:*=*7==9=)()/7.).7)77AB':=='(((((('((((55((,((((+2(2(((4838;.9&+2
@HWI-H225:69:CBJ27ACXX:8:1101:7349:2270 1:Y:0:GGCCTG
CTATAGTCACGTCCAGATACCGTATGCCGCAGCTTGTTTGAATAGAAACTTTCTGAAAAAAAAAATCGATGTC
+
;3)))2:.).:((:(9;=1))77.:))6933''..)(.)))6(..1=(:==(53(77;,;,&505+:866(82
@HWI-H225:69:CBJ27ACXX:8:1101:7403:2306 1:Y:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCAATGCCGACTCGGGCCAGATCGGAAGAGCAAACGCC
+
<6..=?>?;9)9=339>?8??=)<>?>;3>=?;;'(7((((((,3'55(()&&(+(82/&22&+((+(((&&)
@HWI-H225:69:CBJ27ACXX:8:1101:7476:2381 1:N:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATAGAAA
+
DB?CDDD9BDB29BD<B?*9?B=88=@CEDEI@).776766).=37@7).6;>3;>;5;5>?=950;(((+((
@HWI-H225:69:CBJ27ACXX:8:1101:7309:2425 1:N:0:GGCCTG
GATGTCGACTCGAGTCAGATCGGAAGAGCACACGTTGAACTCCAGTCACGGCCTGATCTCGTATTCCGTCTTC
+
FCEFGI=BDB@BFA9DDFEEC=@5@@;6=C2A?A;@;66;>A@C>;>;A;;@B;7(44>A((++((4((+2+(
@HWI-H225:69:CBJ27ACXX:8:1101:7264:2455 1:Y:0:GGCCTG
ACCGGTTCTAGCAAACAGCAATTGATTGACTGAGAATCTGATAAAGCACCAAAAGATCTATAACCCCCCCATA
+
-1==;6/=1>?;9:977..)<3.)..39>?>8>7.::>?====??1=><(,9;<<(((((,,((730059&(+
@HWI-H225:69:CBJ27ACXX:8:1101:7330:2485 1:N:0:GGCCTG
GAATATAGAGGAACTGGCTAGACACCTGCAGGAGGCCGGCCATGAAGTCGAAGCTGGCTCACTCCTCCTGGCT
+
JJIIIJJJJJJJJJGIHIJJGCCGHIJHHHHHFFFDDDDDDDDDDDDDDDDBBBDCCBDDDDDDDDDDDC><?
@HWI-H225:69:CBJ27ACXX:8:1101:7415:2491 1:N:0:GGCCTG
AAGCCAATCTGTCTGTAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCA
+
EC4CEDD>DACBDEBBDEIIIIDIIDDD;@@DAA>=???=9=A?:>:>><???9>>>A??29<<<>AAAAA>>
@HWI-H225:69:CBJ27ACXX:8:1101:7748:2322 1:N:0:GGCCTG
ACCGGTTCTAGCAAACAGCAATTGATTGACTGAGAATCTGATAAAGCACCAAAAGATTTATCCCCCCCCAAAA
+
HGGIIHIIIIJIJIIHIJJGICFGIIIIIIJIIFGHHGHFFFFFFDFEEDEDDDDDACC@C>ACBDDDD@B@B
@HWI-H225:69:CBJ27ACXX:8:1101:7659:2373 1:N:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCGGAAAAAAAAAATCGATGT
+
FFCDG?@?@HHHIDIJIIIIIIEHHGHEHIHIIEHGIHHHH;BDFDFFCC@CCC=>?DDCBDBDBD?@?A1(8
@HWI-H225:69:CBJ27ACXX:8:1101:7968:2280 1:Y:0:GGCCTG
GACTCGAGTCAGATCGGAAGAGCCCACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTCCGTCTTCTGCTT
+
(183=3(0.<=3=13;9(--<()(8=/-..6=1);3).((:=9=;3;<22((28;(2(8(((+3668:6+28<
@HWI-H225:69:CBJ27ACXX:8:1101:7832:2361 1:Y:0:GGCCTG
GATGTCGACTCGAGCCACATCGGAAGAGCACACTTCTGAACTCCACTCACGGGCTGATCTCAGAAGCCATACT
+
A<):1=;?8?A)=4(<8)9)85:=(-==>3(.6).;5(((6;.;;(((5;27'3&)2(+8((+((+31+(((+
@HWI-H225:69:CBJ27ACXX:8:1101:7978:2404 1:N:0:GGCCTG
GATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTT
+
I?FEDDB8@F6BF6888C>=F@@DCE9?CD>?69;;>@@B:;(;>>;:>@8;@<<79:@(:?<2(((40&&(+
@HWI-H225:69:CBJ27ACXX:8:1101:8211:2276 1:N:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCT
+
JJIJIHIJJJIIHHHHGC>BBB;<@ACC?CDDBDDDCCCACDDCACCD<B@B5?C::AA<28((+:.)52?C@
@HWI-H225:69:CBJ27ACXX:8:1101:8048:2303 1:N:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACATCTGAACTCCAGTCACGGCCTGATTTCTGCTTTCGGATTCTGCTTT
+
?DHI=?6?BFDC@DBF;FEG6;=C>6@@7==CE>ADCFFFED>8?:;8<(>(;@,(((((4(&(()4((((+(
@HWI-H225:69:CBJ27ACXX:8:1101:8128:2398 1:Y:0:GGCCTG
TTAAAGACCCATAACTTCACTAGCTCTGGAGAGTTGGCTGGTTTACCAGTACCAGGCATAATTTCCCTCCTGT
+
?B00B?DBDCDECB@DBEEEIA8@@=@))=2=@=?C@:A(,?AA(.6;(-5;;;;??9>A:ADD((,:3<94>
@HWI-H225:69:CBJ27ACXX:8:1101:8028:2467 1:N:0:GGCCTG
TCGCAAGAGCACACGTCTGTCGACTCGAGTCAGATCGGGAGTGAACACGCCTGAACTCCAAGCACGGCCTGAT
+
*1)))0)0B9B(9(-88=().-(-('-(,)..;@(.(33;2(,(,(5?'&(((+:4+(:>((+89&).&)((+
@HWI-H225:69:CBJ27ACXX:8:1101:8492:2256 1:Y:0:GGCCTG
CTCGAGTCAGATCGGAAGAGCACACGGCTGAACTCCAGTCACGGCCTGAACTCGAATGCCGTCTTCTTCTTGT
+
(0/(<(09<)))..37='.(8)(.(-',,'.3-((449;;(('38:66<::<<(((4+(500&((++((+((+
@HWI-H225:69:CBJ27ACXX:8:1101:8365:2316 1:Y:0:GGCCTG
GATGTCGACTCGAGTCAGATCGGAAGAGCAAACGTCTGAACTCCAGTCACGGCCTGATGTAGTACACCGGTCT
+
1(..0;1(-0-(.;)63.):6;3=3'.().).636..,(-(-(-(,,,7(,')&&(++(+(5((+(+3&&)&)
@HWI-H225:69:CBJ27ACXX:8:1101:8293:2346 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAATCGAT
+
JJIJJIIJJJJJJGIHIIJJJJIIIIIIGGGJEGIIGGIFFFHHHFFFFFDDDDDDDDDDDDDDBDDDB@?B<
@HWI-H225:69:CBJ27ACXX:8:1101:8712:2383 1:Y:0:GGCCTG
ATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCTAAACCGTTTTT
+
>7)(8(6658=7'..8).=((9,6;;55325;<25(26::/:2+8(82(&)056-/((5((+((+(+)82(+(
@HWI-H225:69:CBJ27ACXX:8:1101:8715:2465 1:Y:0:GGCCTG
ACAGCGGTCGGCTAGCGGCCCGGGTTGCAGGCGCAGGTGGCACGCAAGAACGCTAGTGAGTGCCTGATGCAAG
+
((2((0((-(-'((..''',3;'',,,(,((&)&&)&+(((+()&&&)((()&((&(+(+(+6(3+8((((((
@HWI-H225:69:CBJ27ACXX:8:1101:8763:2255 1:N:0:GGCCTG
CGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTCCGTCCTCTTCT
+
IA?FCHCE<9=C>DHEGGICHI>9C9CD?6=>BCC@>;>5;53(53;;B92(::(:8+<(((++++&+:(+++
@HWI-H225:69:CBJ27ACXX:8:1101:8920:2420 1:Y:0:GGCCTG
GACAGATCGGGAGAGCACACGTCTGAACTCCACACACGGCCTGAGCTCGTATACCCTCTTCTGTCTGCAACCA
+
4)000*==AA(7'4=23..=<=6)()..))77((,5;'',3'(,+(++&&(3(+33(((+:8(+((((++(((
@HWI-H225:69:CBJ27ACXX:8:1101:8877:2480 1:Y:0:GGCCTG
ACTCGAGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTTATCTCCGAATCCCTCTTCCTCTTG
+
>;(;=74/=)0<1=;8==839>.(.<56(=3=1.:(8(.:;<=,3;;'((76::())(+(++(2(((((+++(
@HWI-H225:69:CBJ27ACXX:8:1101:9145:2369 1:N:0:GGCCTG
TTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTATCTCGAATGCCGACTTCTG
+
)5@=;E=A;=A7?B>;)6=A?B;;=(595<B5<CBCCCCCC@@:ACC59&59(++::@2((((+:5&&0+(+(
@HWI-H225:69:CBJ27ACXX:8:1101:9134:2402 1:Y:0:GGCCTG
TCGGAATAGCACACGACTAAAATCCATACAAATACTCATCTAAGATGCGCGATTCTTCGTGAAAACAAACATA
+
0);4=;(<)7;)..'--;';>1)8=8)))8)))((((((((-(--(3;'''&)+((+(+6268(8+86(86:(
@HWI-H225:69:CBJ27ACXX:8:1101:9249:2486 1:Y:0:GGCCTG
TCAGATCGGAAGAGCAAACGTCTGAACTCCAGTCACGGCCTGATCTCCTATGACATCTTCTTCTTCGAAAAAA
+
?=???4??:==A=27=)=AA.6.>A=A=A77AAA>>''9=/(;--,((,((,(5(,59(3:(+(+((+(2&()
@HWI-H225:69:CBJ27ACXX:8:1101:9295:2485 1:Y:0:GGCCTG
ATTATTTTTCCTGTTATGATCCTGGCCCTGGGAGCAGTCAGAAAAGGTATGGATTACCTCTTCTCCCAGCACA
+
83@;1=8?>983:679)39::..733><937(.;56>?@?<>=;;:=;;)=78:99849(7;4;(:;76;;7(
@HWI-H225:69:CBJ27ACXX:8:1101:9594:2303 1:Y:0:GGCCTG
AGTCACATGGGAAGGGGACAGGTCTGGGGTCCAGTCACGGTCTCTGGCGGAGGGCCTCCGGTCCCCCCCAAAA
+
((8=?).))9>1>?(;'''((,))).((','(-(-,(:,2(7'(((+(&&&&&05:((()&&&&2+&)&59<<
@HWI-H225:69:CBJ27ACXX:8:1101:9606:2483 1:N:0:GGCCTG
TATCGACTCGAGTCAGAACGGAAGAGCACACGTCTGAAATCCAGACACAGCCTCATCTCGCATGCCCCCTTTT
+
8)8?D;(6@;=@8@@=C).4;--'6;).;@C'((5((5(,:(,(((5<(((++++++:@80&(+4((2&&(+(
@HWI-H225:69:CBJ27ACXX:8:1101:9926:2255 1:N:0:GGCCTG
CTCTTCCGACTCGAGTCAGATCGGCAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGACGTCT
+
?00?@</(-@'5(6@)..7;=?=8''95(5;;;(3?A?(5:::AA>3+:3>>B59-(((443(2+3(((++&(
@HWI-H225:69:CBJ27ACXX:8:1101:9786:2276 1:N:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAATCCATGTCCGCTCGGAGCAGGACGGAAACACCAACCGCT
+
??CGGHEGIIGGF>FHAECBGGGGEEIIIHIIHD(.6((.((((''''''&&+((((&)&+&(+((((+&&)&
@HWI-H225:69:CBJ27ACXX:8:1101:9900:2467 1:Y:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTTCAGCTTCTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATG
+
*=1?7?>:AAAA;;?</?B77))/8)=4>)..)=>>).==33=).7>>;2..;>@(6-5;5;'89958=;2++
@HWI-H225:69:CBJ27ACXX:8:1101:10031:2271 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGAGCTCGTATGCCGTCTTCTCCTTCGAAACAA
+
3:)=137>?=1<9799:)6'.;158;;3>??5>>;?/5=8/3,(3(7'3,2(77:95/-6((+(((((+()2+
@HWI-H225:69:CBJ27ACXX:8:1101:10055:2456 1:N:0:GGCCTG
GCTCAGGTGATTATCCTGAACCATCCAGGCCAAATCAGTGCTGGCTACGCTCCTGTTCTGGATTGTCACACAG
+
E:CEEC;???@BBBDDDDIE<??BBDDD8BDIIIEEEC==CAAEDDDDDDDA@AAAAAAAAAAA=ADDAA>??
@HWI-H225:69:CBJ27ACXX:8:1101:10248:2470 1:Y:0:GGCCTG
TCGAGCCAGATCGGAAGAGCAAACGTCTGACCTCCAGTCACGGCCTGAACTCGGATGGCGACATCTACTTGAA
+
.3-.)'-:=>:);/(:/097).)(3.65((((-72(8;;(,8;555++(+86(()2&(8&&&&0/((((((((
@HWI-H225:69:CBJ27ACXX:8:1101:10066:2470 1:Y:0:GGCCTG
TCTCTCCTGAAGTCAGAACGGGAGCACCCCCGTCTCCACCCCAGCTCGGGTCCCAGCTCTGAGCCCGGGTACC
+
.6;>))8=9)).::))))),,',''(,3/327)8:/((+(&&+8(+(&)&+&((+((5((++((++&&&)&56
@HWI-H225:69:CBJ27ACXX:8:1101:10071:2499 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
HIIIIIIIIIIIGIFIIIIIEIDHIIGHGIHHHHHE>BC<',5>ADCA88?(:A805(244(4+>((+:><>B
@HWI-H225:69:CBJ27ACXX:8:1101:10450:2291 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
IIEEIEIIIIIIDDDEIIIIBC>CEEEEDEIEEIDDADD@@?@>AEA>><A?AA?8=8>>>4>:>4(:>A?79
@HWI-H225:69:CBJ27ACXX:8:1101:10423:2308 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
DFFIIIIIIGIIIIIIGHIICFGGIIGIEIHGHHHFFFDDDDDCDEDD@@?3:A?9>BBDDACAC:8@:AB@>
@HWI-H225:69:CBJ27ACXX:8:1101:10399:2309 1:N:0:GGCCTG
TTAGTAATAGATCCCACACATAGCATCATAGAGTAGTATGGCAGCCTCACCACACAAAAAAAAAATCGATGTC
+
IFIIIECFGGIIIIIIIIIIIIIIIIIIIIIIIFIIIIGFIIIIFFFFDDDDCCCCBBBBBBBBB?@?BBBBA
@HWI-H225:69:CBJ27ACXX:8:1101:10313:2333 1:Y:0:GGCCTG
ATGTGATCTGCTCTTGTTCCGTAAACCCCCAATATCAATTTCTGCCACCAAAATATAAAATTCTGCACTCTCT
+
)0)))0)).0))))))/)/.5..'.))6-'',.((((.(((-(::((((,,8(7,52(((((5:(5+6((++(
@HWI-H225:69:CBJ27ACXX:8:1101:10412:2388 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTTCTTTTAAAAAA
+
FHIIJFGIIIIGDCHGGIGGCGFGGGIIEHHGFHHFFFABDBC5;CCABDA?>@8)9ABA@(44@(+4>CC:5
@HWI-H225:69:CBJ27ACXX:8:1101:10360:2392 1:Y:0:GGCCTG
ATTATTTTTCCTGTTATGATCCTGGCCCTGGTAGCAGTCAGAAAAGGTATGGATTACCTCTTCTCCCAGCACG
+
BBBABBBBBBBBBBBABBBBBBBBBABBBB=>BBBBBABBBBBBAAAA>@@@@@@@@@@@AAAB???>?====
@HWI-H225:69:CBJ27ACXX:8:1101:10346:2449 1:Y:0:GGCCTG
CTCGAGTCAAATCTGAGGATAAAACAACTGACCTACCGTCTCGTCCCGTTTTGCTATGACACAAACTTCTTCT
+
1.)(07).).9)0))9)(9))/)9/.(.8)8..<)).','(.(,(,',(,(,3(3(:;0(,(+(((5((+(+(
@HWI-H225:69:CBJ27ACXX:8:1101:10632:2408 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCCCGATCTCGTATCCCCTCCTCTTCTTTCGAAAAA
+
EEI<CDD>D;DD@DDE>ADD=;BCCEDDCDCD@CAC4(5''',,;9A(,(5(,:8(5(((((((+(((+(29<
@HWI-H225:69:CBJ27ACXX:8:1101:10750:2252 1:Y:0:GGCCTG
AGGCAGGTGAATTTCTTGAATTTTATTGTTGGTGGTCTTTTCTACATAAAGAACTGTATTTTAAAGTCACAGC
+
8>9;3((())0).3).>3))0.9))..))9(.-5(93.)8.6)).;))).)(((.((-:;(((4((,(((,((
@HWI-H225:69:CBJ27ACXX:8:1101:10788:2369 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGCTCTCGCATGCCGTCTTCTGCTTGCAAAAAA
+
:???FGFFBA@AFFIBFFEFFFEECDEEFEEAA?>;?<@>>;(-5;A;(',((4&)92?>>(:4>(((+>3?9
@HWI-H225:69:CBJ27ACXX:8:1101:11224:2295 1:Y:0:GGCCTG
GTCGAGTCGAGACCGATCGGAAGAACACACGTCTGAAATCCAGTCACCGACTGAGCTCGTATGCCGTCTTCTT
+
).)6.'.<3;=(,(3'''0&((&+(5(+209++226:6//6<5:<42(()5553++(388((((5&&0(22:/
@HWI-H225:69:CBJ27ACXX:8:1101:11184:2337 1:N:0:GGCCTG
CGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTTTTTTAAA
+
EE;CFB?BBA*?@FD:;B3C(=6@667;CA;7A77?77@>D3=;@;5((,:<8(5<>(&))8+((((+4&(4>
@HWI-H225:69:CBJ27ACXX:8:1101:11117:2381 1:Y:0:GGCCTG
GCAGATCGGAAGAGCACACGTCCGAACTTCAGTCCCCGGCTGATTTCGTAATGCCGCTTTTTCCTTGAAAAAA
+
(((((.)(:-<(((.0(0.'.((''8'()8)...))(,'3',((((,((((((5(&0)&++((+32++6;56:
@HWI-H225:69:CBJ27ACXX:8:1101:11147:2457 1:N:0:GGCCTG
GGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAAAAAAAAATCGATGTCGACT
+
IIFIGGGHIHIIIIIGIIIIIIEIIIHGGHEGIGIIIIIHHHEEEEECCCCCC@BBBBBBBB@?B?AACBB<B
@HWI-H225:69:CBJ27ACXX:8:1101:11497:2395 1:Y:0:GGCCTG
GCTCTTCCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTTCAGTCACGGCCTGAGCTCGTAT
+
?2=??>9>?8??(1.399;;?81;>?8>)696;/93(:((9;;<<<;=<=(+3:=28+05&&)6(((4())(+
@HWI-H225:69:CBJ27ACXX:8:1101:11406:2433 1:Y:0:GGCCTG
CGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCGAATCCCGGCTTGTCT
+
;8A81AA)00>AB79*0<A<A>(...8==.6==AA@@>@;6(.;>6=:6;:;,;5(())(((5(()&&&&(((
@HWI-H225:69:CBJ27ACXX:8:1101:11301:2435 1:N:0:GGCCTG
GAGCTCAGGGCCAGCCTGGTTTACATAGTGAATTCCTGGATAGCCAGAGCTACATAGTGAGACCTTGTCTCAA
+
JJJJIIJIIJJJJIJIJIJGGJBFHIIIAHGJIJJJJJGGGGGHHFFFFFE@CEEEDCDDDDDDDDDDDCCED
@HWI-H225:69:CBJ27ACXX:8:1101:11357:2491 1:N:0:GGCCTG
TGTCGACTCGGGTCAGATCGGAAGAGCACACAACTGAACTCCAGTCACCGCCTGAGCTCGGAAGCCGTCTTCT
+
8809?;ADDICA(;(7.).(5(5'(.(;5=(((,((,5,5:A>4::4>(859929((4>9&5(522&&0(2(4
@HWI-H225:69:CBJ27ACXX:8:1101:11783:2301 1:Y:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTCCCGTCACGGCCTGATCTCCTCTTCCATCCTCTGTTTGTCACAC
+
56))9))60(035.(7;(.7=(6)(.39);)(,.=339',8'(((7(,((++(+2(+((((+(+(+++(5((+
@HWI-H225:69:CBJ27ACXX:8:1101:12064:2289 1:N:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTC
+
EHGIIGIGIGIHIIIIIIIIEGFGHGGIIGIEHEC?C?BB@@CB@B<@B19>?CCCCCBB?@B@BCCBCBBB?
@HWI-H225:69:CBJ27ACXX:8:1101:12116:2299 1:Y:0:GGCCTG
TCGGAAGAGCACACGTCTTAACTCCAGTCACGGCCTGACTTCGTATGCCGTGTTCTCCTTTAAAAAAAGATGT
+
?*0)0=A=(7/=>;7>;A(7...)..).)=A'',9';(((5(5(5,55,8&&(28;(+(3(+8((()5&2(+8
@HWI-H225:69:CBJ27ACXX:8:1101:12161:2332 1:Y:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGCAATCCGGGCTCCGCCCTCAAAACAACAGA
+
1<66(-9(96=()93<>1=9:5))..=3)).5/53'9((-9-((''',,,'&&)((&&+&&+2(((+((+((+
@HWI-H225:69:CBJ27ACXX:8:1101:12108:2478 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
GHIDGGIDEFHIIJJEIIIIFHFHGHGIHHGGHHFFFC@B@??>@ACA8<CCCC5098?CD:>3>42>CC<@<
@HWI-H225:69:CBJ27ACXX:8:1101:12593:2285 1:N:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAA
+
GFEFHE9BGBHIGGG;B8CFG@@@G;C;?.=AE=B@@>CCBBBCC>CC<(555:@<>>BB:>::>@:(>::<@
@HWI-H225:69:CBJ27ACXX:8:1101:12532:2310 1:Y:0:GGCCTG
GTCGACTCGAGCCAGATCGGAAGAACACACGTCTGAACTCCAGTCACGGCCTGCTTTTTTCATCTGCACCCTT
+
)>B(=AA<;(9''-,;3(,,(('((,33+)0++3(88++3((88;+3&&&09(+(+8(()((+((+((((++8
@HWI-H225:69:CBJ27ACXX:8:1101:12908:2384 1:Y:0:GGCCTG
GACAGCCAGTGCAGCTTCTGAGTTGATTCTGAGCAAGGAACAGCTCTATGAGATGTTTCAGAACATTCTTGGG
+
?AABBBBB>ABBBBBBBBBBBB=>A;BBBAAAA>7;;=>>>>>@????B??????>??==::?=;=??;???<
@HWI-H225:69:CBJ27ACXX:8:1101:13236:2260 1:Y:0:GGCCTG
TCAGATCGGAAGAGGACACGTCTGAACTCCAGTCACGGCCCGAAATCTGAGGCCAGTGTTTCCTCTCTAACAA
+
11?9*10)??))B(((8=20@28(.7.=C7).=)))',93''3''55((((((2+2((44<(:3(43(+(((+
@HWI-H225:69:CBJ27ACXX:8:1101:13071:2265 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTCGATGTCGACTCGAGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCC
+
BDHG=F6??;D@89;=FEE@CC4@@;@AEEFDB'9?;?:;AB7<89((:<C?9(8?@>C3::>:(:@C>5<<B
@HWI-H225:69:CBJ27ACXX:8:1101:13059:2292 1:N:0:GGCCTG
GCAGATCGGAAGAGCACACGTCTGAACTACAGTCACGGCCTGATCTCGTATGCCGGCTTCTTCTTGCACAAAT
+
)?D7DB3?D7('8().=7(;...(7A3?);@A?>(.3''988(,:>A(,+3(((&&&0&(((++++(((+2+(
@HWI-H225:69:CBJ27ACXX:8:1101:13105:2335 1:Y:0:GGCCTG
TATCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGAGCTCGTATGCCGTCTTCT
+
((-8;AAA:;=A;>77373=@=;,5555(9(89588????:;=(+3:?9;9;;;(((+50)(+38=.9.9;;:
@HWI-H225:69:CBJ27ACXX:8:1101:13182:2376 1:N:0:GGCCTG
GACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTT
+
FEGBFGIIGHIIGGIIIDHGHIECGEHHHHHEEECECCCCCCCCBBB@=?35>@A<28A(:A&&)+8++4:>A
@HWI-H225:69:CBJ27ACXX:8:1101:13130:2423 1:Y:0:GGCCTG
TCTCAACTGCAGACAACTCGAATGAAGACACGTCATAACTCCAGACACCGCCCCCTCTCTTATTACCCATTCC
+
=-0))0.)))0)7>9)().(.))))))).<),(6(.6((.5;;916(8('')&&&)&22(+((((+((+(5((
@HWI-H225:69:CBJ27ACXX:8:1101:13263:2258 1:N:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGATGTCGACTCGAGGCAGATCGGAAGAGAACACGCCTG
+
;</=CC:7;;=@7C>7=..(9;,3(;;@;?;;<@3<>;@;(525@;08<-5882(38>((088(3:330&(((
@HWI-H225:69:CBJ27ACXX:8:1101:13447:2282 1:Y:0:GGCCTG
ACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCTGTTTGACGCTTCTTTATT
+
659=5<2.9>>?896'37-3().;36'.:3(8=;68;>3==7=;;<<<5233:8((5+((((&&)+&(+(++2
@HWI-H225:69:CBJ27ACXX:8:1101:13345:2391 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCTATGACCGCTTCTTCTTGTAAAAAA
+
11?D9::F8BD<FCFEEFE:==@;D=)7)=?C==A;9?<@BBB5;@A(,(5((,(,0&09::@A34+(4::(0
@HWI-H225:69:CBJ27ACXX:8:1101:13604:2424 1:Y:0:GGCCTG
TCCGAACGCAGGAGCGCACGTCTGAACTCCGGACTCCGCCTGCTCTCGGGTTCCCGCTTTTTGCTTTCGTACT
+
13((((0''--5(((',',',,6(9(5(,((,'&&++()85((5+3+&(&(+(++&))59((&(+((+++(((
@HWI-H225:69:CBJ27ACXX:8:1101:13610:2478 1:N:0:GGCCTG
TTTCAATAAAGAATAACTAAATCCCAAAAAAAAAATCAATGTCGACTAGAGTCAGATCGGAAAAGCACACGTC
+
2?CG<9B<<DFGGCFF@G9?FFBDDFFIIIFFFD?;5(-((-5(('('(((+4:(44>1<?<&((2>((<5<2
@HWI-H225:69:CBJ27ACXX:8:1101:13970:2267 1:Y:0:GGCCTG
TCAGATCGGAAGAGAACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTTCAAAAAA
+
??<;.=?5?<5<7>)<)9837>=?;;?>9)68:><9=/=='((((-903/;02;2&&(8:/((+82+((++20
@HWI-H225:69:CBJ27ACXX:8:1101:14026:2259 1:Y:0:GGCCTG
TCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCCTGATCTCCAACTCCGATTTCCTCTTTTA
+
((((-))09>))(5(7'=6):0.39=9>98==663==66(4-((7((,,(,;((((((++&+((+((((3(+(
@HWI-H225:69:CBJ27ACXX:8:1101:14227:2326 1:N:0:GGCCTG
ATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAAATCCAGTCACGGCCTGATCTTCTAATCCCGCTTC
+
B2B.=;F<@AC=E>B;)))..3;?',(5:<(5;?@?<@A(++:>@>A4+)9@B&((++(((++(((+(()550
@HWI-H225:69:CBJ27ACXX:8:1101:14004:2369 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
HJIJJJJIJJIIGIIJIJJJHIFHIJJJJIGFHHHFFFDDDBC@CEDB<??<@C=598?C>:@:A(+::ABB<
@HWI-H225:69:CBJ27ACXX:8:1101:14359:2446 1:N:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTCTGCCGTCTTCTGCTTGAACAAA
+
?*?GEFFEFI?;AFE=AFFA@6.=A=AECDB@?BD36'=8?@<9AA3>??(8((()&)+((4:>>>(++(+((
@HWI-H225:69:CBJ27ACXX:8:1101:14572:2324 1:N:0:GGCCTG
CACCGCTGCCACCCTGCCACTGCAGGGCGAGGAAGAAGGGGGAGAAGATTGATCAAACTGAATGAAAAACACA
+
JJJJIIIJJJ>?FH()((..))8=@@G5:'9@'5;(:,9?B-5508(((+((++4@C(((+:((+:>(<&8((
@HWI-H225:69:CBJ27ACXX:8:1101:14647:2405 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTCATCTCGTATTCCCACTTCTTCTTTGAAAAAA
+
?D<DD?DDIDC@>@BCDDDA8=3;CAA.?ACA?CA;4>@;3(--;5:=(,((,5(,+(+4:(44:4+(((+29
@HWI-H225:69:CBJ27ACXX:8:1101:14713:2499 1:Y:0:GGCCTG
TCAATGGTGTTGTTCAGATACTGTCTTCTGCAGCTTGTTGGAATGGAAGCTTTCTGGAAAAAAAAAATCGATG
+
:??<=>?:<:=9???8>>???1)).<==?>?>@@;??>?7>=99=39=?(698==>>;:;<</899:;4288:
@HWI-H225:69:CBJ27ACXX:8:1101:14876:2253 1:N:0:GGCCTG
GTCTCTCTCGGAGCAATGGCTACTGTTTCGTTTTCTTTTTTCTGTTTTTGTTGCTTTCAGTGCATGGTGATAA
+
ECGC>BFFEFA80@F>>B@DCF=.8=@@=;(.5?)==AD>3;((.(-;,,(,((,5((5(,:@3((2((4:>(
@HWI-H225:69:CBJ27ACXX:8:1101:14832:2284 1:Y:0:GGCCTG
CCACCCTGGCAAACGTCATCTCTGCTCTGGCTGAAATGGACTCTGCACACAACAAGAACAAAAAAAAAATCCA
+
;<<>63:7((.=>(5.(7(.;>=183)=))))76(66(.3;0;:9(,5(,,7(865287569<<::::5(4+8
@HWI-H225:69:CBJ27ACXX:8:1101:14934:2334 1:N:0:GGCCTG
CACAGTTTGCTTCTTCCCTGACATGTGATATGAGCTCACAGATCAAAGCCCAGGCTTGTTTGATGTTTGCACT
+
HGIIIDHIEIHEHIIIGIG>GCBFHIEHIIICFBHIIGIIGIIIIIIIIIIIHHFFEEEECCCCC3>CACDCC
@HWI-H225:69:CBJ27ACXX:8:1101:14772:2355 1:N:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGGCTGCCGGCTTCTGCTTTTAAAAAAAAAAC
+
DDAA>D0293BDDD68B3@A@.=/777A==)-=??@@A>>5>(((,',,'&)&)+(((+((++(+4:&))&&&
@HWI-H225:69:CBJ27ACXX:8:1101:14968:2368 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCTATGTCCTCCTTTTCCTCCAAAAAA
+
*?DGHCHGGCHDF@C>GHH6.@7@DHH>?CEHEHEE''39'(55;(>3(5,,,,((,(+(4(+(4(+(3+8?@
@HWI-H225:69:CBJ27ACXX:8:1101:15009:2273 1:Y:0:GGCCTG
CGGAAGAGCACACCTCCGAACTCCAGGCACGGCCTCAGCTCATATGCCCCCCTCACCCTGTGAAAACCAAGAC
+
.(-=3(26=<79>()<((-7-(/6>1)6:?9533=(.(-73((--(7;(,,&&0((2((+++(42:88<(273
@HWI-H225:69:CBJ27ACXX:8:1101:15142:2303 1:Y:0:GGCCTG
TATTTATCTTTTCCATTTCCGAAAATCAGAAATATAAAAAACAGCCACCAATCTATAGATTTCTGCACTCTAA
+
)))213)26)2))).))).)-(-<=.8)///))/.;>>1)''(.6=36397(((-73((3(((((,(:;02((
@HWI-H225:69:CBJ27ACXX:8:1101:15398:2362 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATTGCGTCTTCTGCTTCAAAAAAA
+
GGGHEDGGIGIEAGF?.B=F=FFHGG>GGHEEACHED>>?B??;;>@A@B((,5')09<@@(4(4(+(+>A.&
@HWI-H225:69:CBJ27ACXX:8:1101:15484:2453 1:Y:0:GGCCTG
TCAGATCGGAAGAGGACACGTCTGAACTCCAGTCACAGCCTGGTATAGTATGCCGTCGTCTGGCTGAGACATC
+
<.));=7>=9-7:=(<?<13(.)-)).)..))669=((690;(-(-(,(,(5,(',&&&(+(((5(((((5((
@HWI-H225:69:CBJ27ACXX:8:1101:15339:2492 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
GJJJJIIJJJJJGCGH@FHIHHHIGIGIJJJIIIHHHHFDCDDCDEDD?BD?CC82;ABAC(4:>((4>CBBB
@HWI-H225:69:CBJ27ACXX:8:1101:15309:2498 1:Y:0:GGCCTG
AAAGATCGGACGAGCACACGCCTGAACGAAAGTCACGGCCTGGACTCGTATTCCCTCTTCTTCTTTGAAAACC
+
)):8(.)(7-(7='536=0='56()))('(''(-:=',3;2((((+((+8((4/+2:(86(+3(3+((5/3+2
@HWI-H225:69:CBJ27ACXX:8:1101:15610:2405 1:N:0:GGCCTG
GCTCTTCCGATGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTCGATGTCGACTCGAGTCAGATCGGAAGA
+
@EDHHIIIJJJJJIJJJJHFHFFFFFDEDDDDDDBDDCBDBBBBDDDDDDBCDDDDDDDDCDCDCCCBBBDDD
@HWI-H225:69:CBJ27ACXX:8:1101:15564:2435 1:Y:0:GGCCTG
GTTCTTCCGCTTTCGACTCTAGTCAGATCGGTAGATCACTCGTCTGAACTCCAGTAACGGCCATATATCCCGT
+
=-(((0..-(7-<.9(=;?81;..).)...',.'((..-(((,,,(,,7(,(,(5((+80&-&+826=/(+(+
@HWI-H225:69:CBJ27ACXX:8:1101:15693:2484 1:Y:0:GGCCTG
TTAGAAATAGATCCCACACATAGCATCATAGTATAGGATGGAAGACTCACCACACACAAAAAAAATCGATGTC
+
93:>)9>.9)).)9)=?((;>).)).)::)))).:))..(,..((-7937((,5;8'2(6688993:-/(8+(
@HWI-H225:69:CBJ27ACXX:8:1101:15889:2484 1:Y:0:GGCCTG
CTCTTCCGACTCCAGTCCGATCGGAACAGCCGACGTCTGGAGTCCAAGCACGGCTTCAGCCCGTGAAACCGGG
+
?/9()=)((-9=)66..)-'(-'',,'((,(',',58966:(43(3+(+2+58&&)(+(++66&&((+((&&&
@HWI-H225:69:CBJ27ACXX:8:1101:16051:2308 1:Y:0:GGCCTG
TCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGAACTCGAAAGCACTGCTTCTGTTTTAAAATTAGTAA
+
)1<=??8(33).9.-.<);88);):9>).))'=3==<7(((-,((('(,(7((5/(((+5++((+((+((((+
@HWI-H225:69:CBJ27ACXX:8:1101:16175:2359 1:Y:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTTTGAACTCAAGTCACGGCCTGATCCCGTATGCCGTCTTCT
+
()().(-(,,(,((-((-(;'8(,5((,(+()+((+58((8(3(((++5&550+(++(3885(((+&&)2((+
@HWI-H225:69:CBJ27ACXX:8:1101:16099:2416 1:Y:0:GGCCTG
GGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCC
+
1?:::=6>A<AA===B9>>BA=;=>=375>5=>@@=????;>?=>9>?6==;998(((892+3838&&)((+(
@HWI-H225:69:CBJ27ACXX:8:1101:16011:2425 1:Y:0:GGCCTG
TCGGAAGAGCACACGTCTCGATGTTGACTCGAGTCAGATCGGGAGAGTACACGGCTGAAATCCAGTCACGGAC
+
))(.<;'(().5=('6=A37=>)...7;>(((3(555((5;=/=-90+8(838&99&(((++(338:3(&&&0
@HWI-H225:69:CBJ27ACXX:8:1101:16175:2489 1:Y:0:GGCCTG
TATGTGACTTTAGCATGTTTAATAATGATGACAATAAAAAAAGCCCTCAAATGGGGGCATTGAACCTCTAAAA
+
BBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBBAA@>>@@@???B??==A===?????==<?????
@HWI-H225:69:CBJ27ACXX:8:1101:16463:2274 1:Y:0:GGCCTG
TCAGAAGGGGAAACAACACCCTTGTCCACCAGGGACGCACCGGAAGAGCAAACGCCTCCAATCCCCACACCAC
+
1**11**)))0)0**00((-'(..=7).7((.(',,',',3''''&(+(+(((&&)(&+33(39(((&+2((+
@HWI-H225:69:CBJ27ACXX:8:1101:16405:2431 1:Y:0:GGCCTG
GTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCACCGACACCAAAGTGTGAGTCTGAAAA
+
A171?*:==:)04>=)05094..8(.=;)=AB==>3>'=',,((-5(-,(8'')(+(+(5(+(((+(+(5;??
@HWI-H225:69:CBJ27ACXX:8:1101:16730:2448 1:N:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGGATGCCGTCCTCTTCTTTTAAAAAA
+
FGEIIIIIIIFBB?DFEBEFCFC@CFDCFFFEEFEFFEDC?C;>B5@5('(,9@8')&+++(((+(++:(4:9
@HWI-H225:69:CBJ27ACXX:8:1101:16859:2317 1:N:0:GGCCTG
GGAACTCCTTTTCCTGCTGCGTCGGAATTGTCAGACAAATAAATCTCTGAGAAACATCAAAAAAAAAATCGAT
+
B?DFGIEEFIIIIC?DE@=FF4;=B<;=@CA>AAAB;?BAABB>ABB>:>>@ABBBBBBBBBBBBBBB8:0<8
@HWI-H225:69:CBJ27ACXX:8:1101:16941:2410 1:Y:0:GGCCTG
CTCGAGTCAGATCGGAAGAGCACACGTCTGAACTACAGTCACGGCCTGATCTCGTTTGTCGTCCTCTTCTTGT
+
2(;57;3;??<=;94;;/3(=????5(9=8147;(7;(57:3;'0895((+4:(+((++6&(((((+(6(3(+
@HWI-H225:69:CBJ27ACXX:8:1101:17022:2348 1:N:0:GGCCTG
CCACCCTGGGAAAGGTCATCTCTGCTCTGGCTGAAATGGACTCTGGACCCAACAAGAACAAGAAAAAAAAATC
+
IIIIIIIIIIIIIIIGHIIIIIHIIIIIIIIIIIDHIIIIFFHHFEEEEECCCBBCCCCCCCCCCCBBBBB?C
@HWI-H225:69:CBJ27ACXX:8:1101:17024:2418 1:Y:0:GGCCTG
GTCAGATCGGAAGCGCACACGTCTGAACTCCAGACACGGCCTGATCTCGTATGCCGTCTTCTGCTTTAAACAA
+
)11?ABAA;41?<)84=64>A867;==>A;7A>),6;/9393(5(5=>=8(53;?;;;97=?38:;+((+((+
@HWI-H225:69:CBJ27ACXX:8:1101:17372:2497 1:N:0:GGCCTG
TGTCGACTCGAGTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCCTCTCCCCTCTTCT
+
(.=FGGIIHFHE@DEECCCCBB;9?BCC<CBBB@?B:@C3>ACA:@CC-5>B<?9>::>(((+(3>&()3(+(
@HWI-H225:69:CBJ27ACXX:8:1101:17504:2345 1:N:0:GGCCTG
GCTCAGGTGATTATCCTGAACCATCCAGGCCAAATCAGTGCTGGCTACGCTCCTGTTCTGGATTGTCACACAG
+
EGHJJIICDDHIIJIIIJJIHGFHGIIGJJGIJGGIGGHJIGEHFHGDFFDDDACCCDECDCACCCDDDDDD?
@HWI-H225:69:CBJ27ACXX:8:1101:17521:2361 1:N:0:GGCCTG
CTGGCAACCAAATCCGAGAAGGTTGTTCCAGAGAAGGGGACTGCAGACAAGAAGCCAGCGGTAGGCAAGAAAG
+
F<?ECHGIGGGG>CD?G@FGGD3=@'.87=@D;)?>E<=69;?CCCCC=,,9CCC@C2805995?CCB<3<<A
@HWI-H225:69:CBJ27ACXX:8:1101:17742:2367 1:N:0:GGCCTG
TACTAATTACACTAATCGCCACTTCTATAACAGCTATGTACAGCATACGAATCATTTACTTCGTAACAATAAC
+
IJJJJJJJIHIJJIGFHIJJJIGIIIGIEHHIHIHHHHHHHFFFFFFEEDDDDDDEFEEDDCDDDBDDDDDDD
@HWI-H225:69:CBJ27ACXX:8:1101:17725:2412 1:N:0:GGCCTG
TCAGATTGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA
+
EEEEDDIIIIIADD>B;B@@9BCCBD)7=CE>@;CAADDD>?;AAAA???;5-:='8(85((4(:(((4>85>
@HWI-H225:69:CBJ27ACXX:8:1101:17507:2464 1:Y:0:GGCCTG
TCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCCTGATCTCGTATCCCGGCCTCCTCCTCCAGAAAA
+
0?:AB<AAAAAAA>))=ABA.6);AAAA>>@@;>@>=9;==85,:9958(+(8:(&5&&(((++(((+((3((
@HWI-H225:69:CBJ27ACXX:8:1101:17970:2417 1:Y:0:GGCCTG
AGGCAGGCGGCTTT