##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	fastq_runid_64766c2b525372b212a266fe25ce856d398c991f_38_fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	172-44150
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	5.24975	5.0	4.0	7.0	3.0	8.0
2	7.69125	7.0	4.0	10.0	3.0	14.0
3	8.1555	8.0	5.0	11.0	3.0	14.0
4	9.1155	8.0	5.0	12.0	3.0	16.0
5	9.6505	9.0	6.0	12.0	4.0	16.0
6	8.787	8.0	5.0	11.0	4.0	15.0
7	11.03725	10.0	6.0	15.0	4.0	19.0
8	10.84	9.0	6.0	14.0	4.0	20.0
9	13.3985	12.0	7.0	18.0	5.0	25.0
10-999	16.822040969188535	15.15050505050505	9.777777777777779	22.83939393939394	6.045454545454546	30.181818181818183
1000-1999	16.891904288311675	15.298	9.769	22.91	6.123	30.281
2000-2999	16.97882749573465	15.425	9.813	22.979	6.258	30.327
3000-3999	16.971539637228044	15.411	9.756	22.976	6.235	30.347
4000-4999	16.92411874185914	15.359	9.697	22.92	6.232	30.245
5000-5999	16.8465690481979	15.271	9.604	22.831	6.152	30.145
6000-6999	16.98477165857214	15.382	9.714	22.986	6.246	30.263
7000-7999	16.92266779617275	15.339	9.68	22.841	6.244	30.1
8000-8999	16.9595378107283	15.37	9.696	22.874	6.284	30.178
9000-9999	17.021641892424405	15.449	9.743	22.962	6.293	30.17
10000-10999	17.054495565606327	15.486	9.747	23.022	6.246	30.176
11000-11999	17.005451264911375	15.381	9.642	22.935	6.156	30.192
12000-12999	16.868978945467457	15.25	9.588	22.735	6.174	29.704
13000-13999	16.790253871036477	15.102	9.417	22.626	6.017	29.781
14000-14999	17.09769397060867	15.43	9.746	22.86	6.199	29.924
15000-15999	17.015140000037192	15.228260869565217	9.66304347826087	22.516304347826086	6.266304347826087	29.032608695652176
16000-16999	16.92819719204156	NaN	NaN	NaN	NaN	NaN
17000-17999	17.39549565540164	NaN	NaN	NaN	NaN	NaN
18000-18999	17.779153905270874	NaN	NaN	NaN	NaN	NaN
19000-19999	17.37072847836203	NaN	NaN	NaN	NaN	NaN
20000-20999	17.853805941213256	NaN	NaN	NaN	NaN	NaN
21000-21999	17.459669581217085	NaN	NaN	NaN	NaN	NaN
22000-22999	17.39496613396131	NaN	NaN	NaN	NaN	NaN
23000-23999	16.991427464387456	NaN	NaN	NaN	NaN	NaN
24000-24999	16.325388696593276	NaN	NaN	NaN	NaN	NaN
25000-25999	16.49651475748195	NaN	NaN	NaN	NaN	NaN
26000-26999	16.69925	NaN	NaN	NaN	NaN	NaN
27000-27999	16.610254166666667	NaN	NaN	NaN	NaN	NaN
28000-28999	17.05658927738929	NaN	NaN	NaN	NaN	NaN
29000-29999	16.191172727272736	NaN	NaN	NaN	NaN	NaN
30000-30999	16.824513888888895	NaN	NaN	NaN	NaN	NaN
31000-31999	16.489944047619044	NaN	NaN	NaN	NaN	NaN
32000-32999	16.025349999999996	NaN	NaN	NaN	NaN	NaN
33000-33999	18.33108333333333	NaN	NaN	NaN	NaN	NaN
34000-34999	20.639	NaN	NaN	NaN	NaN	NaN
35000-35999	19.6395	NaN	NaN	NaN	NaN	NaN
36000-36999	20.3425	NaN	NaN	NaN	NaN	NaN
37000-37999	19.1405	NaN	NaN	NaN	NaN	NaN
38000-38999	20.715	NaN	NaN	NaN	NaN	NaN
39000-39999	19.521	NaN	NaN	NaN	NaN	NaN
40000-40999	19.361	NaN	NaN	NaN	NaN	NaN
41000-41999	21.51	NaN	NaN	NaN	NaN	NaN
42000-42999	20.043	NaN	NaN	NaN	NaN	NaN
43000-43999	20.988	NaN	NaN	NaN	NaN	NaN
44000-44150	18.483443708609272	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
8	14.0
9	118.0
10	142.0
11	150.0
12	159.0
13	218.0
14	309.0
15	394.0
16	458.0
17	493.0
18	595.0
19	494.0
20	303.0
21	126.0
22	24.0
23	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	2.825	7.925	79.85	9.4
2	10.925	2.1	76.85	10.125
3	69.55	13.875000000000002	5.375	11.200000000000001
4	30.275000000000002	17.125	48.5	4.1000000000000005
5	11.5	10.549999999999999	74.125	3.8249999999999997
6	33.550000000000004	37.8	22.05	6.6000000000000005
7	15.299999999999999	12.775	53.625	18.3
8	23.7	25.85	31.574999999999996	18.875
9	12.225	12.875	28.975	45.925
10-999	21.592876937077644	28.129325330392756	29.227075769608803	21.0507219629208
1000-1999	21.985398444327082	28.13432786623469	28.72383985607938	21.156433833358847
2000-2999	21.73678446627617	28.391337404655044	28.73224928261349	21.139628846455295
3000-3999	21.81338217864454	28.216455454899585	28.980630402890935	20.989531963564932
4000-4999	21.84174819681497	28.164713003676084	28.904520459901452	21.0890183396075
5000-5999	22.056224031010977	27.904471553169103	28.992101684059048	21.04720273176088
6000-6999	21.76356401542749	28.02677902717134	29.121682554401268	21.087974402999905
7000-7999	21.943777452415812	28.219619326500734	28.8307467057101	21.005856515373353
8000-8999	21.8613167468446	28.039760940602036	29.036184650411634	21.06273766214173
9000-9999	22.337633057957714	27.59110746090793	28.8898819588708	21.181377522263556
10000-10999	22.213148990290076	27.613442775282635	28.659523843045605	21.513884391381684
11000-11999	21.82347024764183	28.084377633403108	28.924891945524234	21.167260173430833
12000-12999	22.362669433815423	27.849406576301966	28.940268499902714	20.847655489979893
13000-13999	22.423776884897187	27.989836917986295	28.505376979437486	21.081009217679036
14000-14999	21.966797846835846	28.06079522323246	29.19075405980006	20.781652870131634
15000-15999	22.077374172045733	27.779184069527062	29.417162384508288	20.726279373918914
16000-16999	21.939206304531382	28.914105877235624	28.308983445487808	20.837704372745183
17000-17999	20.697470052264002	29.41738741655489	29.139879438200516	20.74526309298059
18000-18999	21.419697311949403	28.659362999774114	29.111136209622767	20.809803478653716
19000-19999	21.597679002502602	28.604965340065995	28.706841184417424	21.090514473013975
20000-20999	21.194490029340095	29.315231985678054	29.374906758167985	20.115371226813863
21000-21999	20.66234288010102	29.50984331056649	29.294610572232106	20.533203237100384
22000-22999	20.98124098124098	28.12770562770563	29.404761904761905	21.486291486291485
23000-23999	21.988903444555753	27.637358403328964	27.891654465592975	22.48208368652231
24000-24999	22.122037227157076	28.59438468062313	27.643619158148464	21.63995893407133
25000-25999	21.75931167213857	29.372806521000793	27.28970904562436	21.57817276123627
26000-26999	21.1625	29.14375	29.493750000000002	20.200000000000003
27000-27999	20.263157894736842	28.91604010025063	29.442355889724308	21.37844611528822
28000-28999	20.408763417144538	30.36318188501691	29.12071754153801	20.107337156300545
29000-29999	22.265001408582965	29.30791623626632	27.814818292797444	20.612264062353272
30000-30999	24.00382912528419	26.720114873758526	27.13892545171712	22.137130549240158
31000-31999	24.265283671224267	27.235580700927237	27.644192990727646	20.854942637120853
32000-32999	24.191798654805815	28.921674983727492	25.29832935560859	21.588197005858103
33000-33999	23.23873121869783	26.67779632721202	28.480801335559264	21.602671118530886
34000-34999	21.75	30.099999999999998	29.349999999999998	18.8
35000-35999	23.45	29.95	26.05	20.549999999999997
36000-36999	19.15	33.1	30.65	17.1
37000-37999	20.324379118094274	27.977698935631018	30.359858084135833	21.338063862138874
38000-38999	19.3	25.6	36.6	18.5
39000-39999	27.0	28.4	25.0	19.6
40000-40999	26.6	23.0	24.7	25.7
41000-41999	12.8	24.4	25.6	37.2
42000-42999	16.900000000000002	30.0	26.5	26.6
43000-43999	19.8	29.099999999999998	26.900000000000002	24.2
44000-44150	30.4635761589404	23.178807947019866	26.490066225165563	19.867549668874172
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.125
15	0.125
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.25
23	0.34090909090909094
24	0.0
25	0.75
26	0.825
27	1.5678571428571428
28	1.3833333333333333
29	1.9305916305916306
30	3.604004329004329
31	6.255375180375181
32	10.64408369408369
33	14.922926746456152
34	15.413439011968416
35	22.289932732034245
36	29.106857548057075
37	28.559156841669047
38	29.586585776053152
39	34.98853620274679
40	29.25856039427737
41	34.86950241338606
42	35.32553210021683
43	35.88073608809246
44	32.18916508707945
45	29.904887840709694
46	28.79514147325484
47	26.195053041787634
48	22.239217496819155
49	22.000849224948972
50	16.52310015740288
51	12.954693461603794
52	11.886876362450437
53	11.657771883287369
54	8.161408815725704
55	6.683055234825496
56	4.602958152958154
57	5.0223735046315685
58	3.340847442617702
59	2.59336917562724
60	1.659001782531194
61	1.3333333333333333
62	0.8409090909090909
63	0.3333333333333333
64	0.5429292929292929
65	0.0
66	0.0
67	0.0
68	0.26785714285714285
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-999	0.0
1000-1999	0.0
2000-2999	0.0
3000-3999	0.0
4000-4999	0.0
5000-5999	0.0
6000-6999	0.0
7000-7999	0.0
8000-8999	0.0
9000-9999	0.0
10000-10999	0.0
11000-11999	0.0
12000-12999	0.0
13000-13999	0.0
14000-14999	0.0
15000-15999	0.0
16000-16999	0.0
17000-17999	0.0
18000-18999	0.0
19000-19999	0.0
20000-20999	0.0
21000-21999	0.0
22000-22999	0.0
23000-23999	0.0
24000-24999	0.0
25000-25999	0.0
26000-26999	0.0
27000-27999	0.0
28000-28999	0.0
29000-29999	0.0
30000-30999	0.0
31000-31999	0.0
32000-32999	0.0
33000-33999	0.0
34000-34999	0.0
35000-35999	0.0
36000-36999	0.0
37000-37999	0.0
38000-38999	0.0
39000-39999	0.0
40000-40999	0.0
41000-41999	0.0
42000-42999	0.0
43000-43999	0.0
44000-44150	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-999	2222.0
1000-1999	683.0
2000-2999	290.0
3000-3999	150.0
4000-4999	122.0
5000-5999	103.0
6000-6999	67.0
7000-7999	41.0
8000-8999	53.0
9000-9999	42.0
10000-10999	26.0
11000-11999	38.0
12000-12999	17.0
13000-13999	25.0
14000-14999	19.0
15000-15999	15.0
16000-16999	16.0
17000-17999	14.0
18000-18999	7.0
19000-19999	9.0
20000-20999	2.0
21000-21999	7.0
22000-22999	5.0
23000-23999	2.0
24000-24999	5.0
25000-25999	4.0
26000-26999	0.0
27000-27999	1.0
28000-28999	4.0
29000-29999	1.0
30000-30999	2.0
31000-31999	3.0
32000-32999	1.0
33000-33999	2.0
34000-34999	0.0
35000-35999	0.0
36000-36999	0.0
37000-37999	1.0
38000-38999	0.0
39000-39999	0.0
40000-40999	0.0
41000-41999	0.0
42000-42999	0.0
43000-43999	0.0
44000-44151	1.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	99.925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	99.97498123592695	99.9
2	0.0	0.0
3	0.0	0.0
4	0.02501876407305479	0.1
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-999	0.0	0.0	0.0	0.0	0.0
1000-1999	0.0	0.0	0.0	0.0	0.0
2000-2999	0.0	0.0	0.0	0.0	0.0
3000-3999	0.0	0.0	0.0	0.0	0.0
4000-4999	0.0	0.0	0.0	0.0	0.0
5000-5999	0.0	0.0	0.0	0.0	0.0
6000-6999	0.0	0.0	0.0	0.0	0.0
7000-7999	0.0	0.0	0.0	0.0	0.0
8000-8999	0.0	0.0	0.0	0.0	0.0
9000-9999	0.0	0.0	0.0	0.0	0.0
10000-10999	0.0	0.0	0.0	0.0	0.0
11000-11999	0.0	0.0	0.0	0.0	0.0
12000-12999	0.0	0.0	0.0	0.0	0.0
13000-13999	0.0	0.0	0.0	0.0	0.0
14000-14999	0.0	0.0	0.0	0.0	0.0
15000-15999	0.0	0.0	0.0	0.0	0.0
16000-16999	0.0	0.0	0.0	0.0	0.0
17000-17999	0.0	0.0	0.0	0.0	0.0
18000-18999	0.0	0.0	0.0	0.0	0.0
19000-19999	0.0	0.0	0.0	0.0	0.0
20000-20999	0.0	0.0	0.0	0.0	0.0
21000-21999	0.0	0.0	0.0	0.0	0.0
22000-22999	0.0	0.0	0.0	0.0	0.0
23000-23999	0.0	0.0	0.0	0.0	0.0
24000-24999	0.0	0.0	0.0	0.0	0.0
25000-25999	0.0	0.0	0.0	0.0	0.0
26000-26999	0.0	0.0	0.0	0.0	0.0
27000-27999	0.0	0.0	0.0	0.0	0.0
28000-28999	0.0	0.0	0.0	0.0	0.0
29000-29999	0.0	0.0	0.0	0.0	0.0
30000-30999	0.0	0.0	0.0	0.0	0.0
31000-31999	0.0	0.0	0.0	0.0	0.0
32000-32999	0.0	0.0	0.0	0.0	0.0
33000-33999	0.0	0.0	0.0	0.0	0.0
34000-34999	0.0	0.0	0.0	0.0	0.0
35000-35999	0.0	0.0	0.0	0.0	0.0
36000-36999	0.0	0.0	0.0	0.0	0.0
37000-37999	0.0	0.0	0.0	0.0	0.0
38000-38999	0.0	0.0	0.0	0.0	0.0
39000-39999	0.0	0.0	0.0	0.0	0.0
40000-40999	0.0	0.0	0.0	0.0	0.0
41000-41999	0.0	0.0	0.0	0.0	0.0
42000-42999	0.0	0.0	0.0	0.0	0.0
43000-43999	0.0	0.0	0.0	0.0	0.0
44000-44138	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATGTAC	15	1.0158165E-6	473.35004	3
TTGATAC	10	1.9771003E-4	473.35	1
GATACTT	10	1.9771003E-4	473.35	3
TTATACT	10	1.9771003E-4	473.35	4
TGTTACT	10	1.9771003E-4	473.35	2
TGTTGTA	70	0.0	371.91788	2
GTTGTAC	65	0.0	364.11536	3
ATGTACT	20	3.2053285E-6	355.0125	4
TATACTT	20	3.2053285E-6	355.0125	5
TGTACTT	140	0.0	321.20178	5
TTGATGC	15	6.662547E-4	315.56668	1
TTATGCT	15	6.662547E-4	315.56668	4
TTGTACT	105	0.0	315.56668	4
TGTTATG	15	6.662547E-4	315.56668	2
TTGGTAC	15	6.662547E-4	315.56668	1
GTTATGC	15	6.662547E-4	315.56668	3
GTTATAC	15	6.662547E-4	315.56668	3
TACTTCG	160	0.0	310.63593	7
GTACTTC	150	0.0	299.78833	6
ACTTCGT	160	0.0	295.84375	8
>>END_MODULE