##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	beer_reads_fastq_
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1876
Total Bases	720.8 kbp
Sequences flagged as poor quality	0
Sequence length	130-2327
%GC	29
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	5.907782515991471	5.0	3.0	8.0	2.0	11.0
2	5.5863539445629	4.0	3.0	6.0	2.0	11.0
3	6.294776119402985	5.0	3.0	7.0	2.0	13.0
4	5.87046908315565	5.0	3.0	7.0	2.0	11.0
5	6.226012793176972	4.0	3.0	8.0	2.0	14.0
6	6.850213219616204	5.0	3.0	9.0	2.0	15.0
7	7.815565031982943	5.0	3.0	11.0	2.0	18.0
8	8.99360341151386	7.0	3.0	13.0	2.0	19.0
9	10.028784648187633	8.0	4.0	15.0	3.0	20.0
10-49	11.523307569296374	10.35	5.6	16.125	3.125	21.55
50-99	13.596023454157782	11.94	6.06	19.66	3.4	26.42
100-149	15.933607664897675	14.7	7.66	22.8	4.02	29.38
150-199	15.877262363712823	14.56	7.6	22.98	4.0	29.48
200-249	15.770034680683215	14.46	7.36	22.74	4.0	29.46
250-299	15.79629127622187	14.38	7.2	22.88	4.0	29.68
300-349	15.802463297025021	14.36	7.18	22.88	4.0	29.72
350-399	16.0993689851264	14.82	7.28	23.32	3.98	30.06
400-449	16.332638081339805	15.02	7.78	23.5	4.1	30.0
450-499	15.66396727525955	14.3	7.02	22.66	3.82	29.32
500-549	15.962084975660703	14.58	7.22	23.06	3.96	29.78
550-599	15.76946299974641	14.1	7.12	22.8	3.86	29.62
600-649	15.826695632802794	14.38	7.28	22.76	3.92	29.4
650-699	15.927721160313945	14.24	7.04	23.26	3.86	29.84
700-749	15.917378999471689	14.48	7.2	22.64	3.88	29.66
750-799	15.981551105060321	14.34	7.14	22.44	3.8	30.2
800-849	16.08378751169033	14.571428571428571	8.142857142857142	23.0	4.857142857142857	29.142857142857142
850-899	16.98405362785197	NaN	NaN	NaN	NaN	NaN
900-949	16.55795808357575	NaN	NaN	NaN	NaN	NaN
950-999	17.41259849978607	NaN	NaN	NaN	NaN	NaN
1000-1049	17.08289838316756	NaN	NaN	NaN	NaN	NaN
1050-1099	16.918844782814826	NaN	NaN	NaN	NaN	NaN
1100-1149	18.102164405987224	NaN	NaN	NaN	NaN	NaN
1150-1199	16.650944844880325	NaN	NaN	NaN	NaN	NaN
1200-1249	15.75858559845952	NaN	NaN	NaN	NaN	NaN
1250-1299	15.079428571428569	NaN	NaN	NaN	NaN	NaN
1300-1349	15.07000447196423	NaN	NaN	NaN	NaN	NaN
1350-1399	18.475001527883887	NaN	NaN	NaN	NaN	NaN
1400-1449	14.3629126984127	NaN	NaN	NaN	NaN	NaN
1450-1499	11.965	NaN	NaN	NaN	NaN	NaN
1500-1549	11.965	NaN	NaN	NaN	NaN	NaN
1550-1599	16.66	NaN	NaN	NaN	NaN	NaN
1600-1649	16.898333333333333	NaN	NaN	NaN	NaN	NaN
1650-1699	14.883333333333335	NaN	NaN	NaN	NaN	NaN
1700-1749	16.83	NaN	NaN	NaN	NaN	NaN
1750-1799	16.45	NaN	NaN	NaN	NaN	NaN
1800-1849	10.14	NaN	NaN	NaN	NaN	NaN
1850-1899	16.29	NaN	NaN	NaN	NaN	NaN
1900-1949	11.87	NaN	NaN	NaN	NaN	NaN
1950-1999	15.3	NaN	NaN	NaN	NaN	NaN
2000-2049	19.42	NaN	NaN	NaN	NaN	NaN
2050-2099	10.64	NaN	NaN	NaN	NaN	NaN
2100-2149	14.36	NaN	NaN	NaN	NaN	NaN
2150-2199	13.74	NaN	NaN	NaN	NaN	NaN
2200-2249	13.14	NaN	NaN	NaN	NaN	NaN
2250-2299	11.72	NaN	NaN	NaN	NaN	NaN
2300-2327	9.964285714285714	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
10	11.0
11	124.0
12	271.0
13	351.0
14	336.0
15	295.0
16	223.0
17	142.0
18	57.0
19	47.0
20	13.0
21	3.0
22	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.54157782515991	3.14498933901919	7.835820895522389	54.47761194029851
2	35.76759061833689	35.501066098081026	27.452025586353944	1.279317697228145
3	30.2771855010661	14.17910447761194	53.304904051172706	2.2388059701492535
4	28.78464818763326	17.11087420042644	49.30703624733476	4.797441364605544
5	29.47761194029851	23.560767590618337	36.30063965884862	10.660980810234541
6	17.537313432835823	24.733475479744136	42.91044776119403	14.818763326226012
7	12.686567164179104	21.85501066098081	29.744136460554373	35.714285714285715
8	11.567164179104477	11.513859275053305	38.965884861407254	37.953091684434966
9	12.473347547974413	5.063965884861407	57.08955223880597	25.37313432835821
10-49	22.919776119402986	16.892324093816633	42.26279317697228	17.925106609808104
50-99	18.872068230277186	22.64818763326226	35.52345415778252	22.956289978678036
100-149	12.911794828027654	36.965520184347525	37.700563284117095	12.422121703507724
150-199	12.750988660447026	37.04690948021587	37.76573663396622	12.436365225370883
200-249	12.606820854566836	37.485953220959104	37.75904873905658	12.148177185417483
250-299	12.730335712673025	37.463311276680734	37.54841174733844	12.257941263307803
300-349	12.847596959746493	37.42221405772808	37.57606374428804	12.154125238237388
350-399	12.743347287684065	37.50402529348049	37.45718551479844	12.295441904037004
400-449	12.530939451032546	37.48199046880195	37.64453803243563	12.342532047729875
450-499	12.751582052624066	37.431602988057286	37.688537850311654	12.128277109006994
500-549	12.461824061321037	38.128031618659804	37.51721659979639	11.892927720222769
550-599	12.607255757940688	38.318530784284214	37.332530483215415	11.741682974559687
600-649	11.958879562388002	38.40422521927756	38.37593134018674	11.260963878147694
650-699	12.294343024566148	38.51701600180302	38.03245436105477	11.156186612576064
700-749	11.840499118404992	38.05777838057779	39.48189339481893	10.61982910619829
750-799	10.219106047326907	38.965819456617	40.89395267309378	9.921121822962315
800-849	10.47910741632028	37.956683439072414	42.00393786917523	9.560271275432072
850-899	10.056925996204933	40.03795066413662	40.55299539170507	9.352127947953374
900-949	10.118475824527698	38.84085814921549	40.85814921549792	10.182516810758885
950-999	10.011164867882396	39.48641607740975	39.59806475623372	10.904354298474136
1000-1049	9.659335920655456	37.990513152220785	43.03579128934886	9.314359637774903
1050-1099	9.173387096774194	38.306451612903224	44.354838709677416	8.16532258064516
1100-1149	8.156028368794328	39.83451536643026	44.148936170212764	7.860520094562648
1150-1199	8.48310567936736	40.2588066139468	41.624730409777136	9.633357296908699
1200-1249	9.760132340777503	34.24317617866005	48.13895781637717	7.857733664185278
1250-1299	12.062256809338521	35.40856031128405	42.217898832684824	10.311284046692606
1300-1349	11.514522821576763	32.572614107883815	42.738589211618255	13.174273858921163
1350-1399	9.733124018838305	35.00784929356358	43.79905808477237	11.459968602825747
1400-1449	8.923884514435695	37.79527559055118	42.78215223097113	10.498687664041995
1450-1499	16.289592760180994	33.93665158371041	34.38914027149321	15.384615384615385
1500-1549	12.0	34.5	39.0	14.499999999999998
1550-1599	9.0	36.0	45.5	9.5
1600-1649	13.043478260869565	31.05590062111801	45.3416149068323	10.559006211180124
1650-1699	9.25925925925926	41.66666666666667	43.51851851851852	5.555555555555555
1700-1749	10.0	41.0	36.0	13.0
1750-1799	16.0	38.0	35.0	11.0
1800-1849	12.0	39.0	37.0	12.0
1850-1899	9.0	49.0	32.0	10.0
1900-1949	17.647058823529413	36.76470588235294	32.35294117647059	13.23529411764706
1950-1999	20.0	32.0	28.000000000000004	20.0
2000-2049	22.0	34.0	32.0	12.0
2050-2099	28.000000000000004	22.0	28.000000000000004	22.0
2100-2149	24.0	26.0	32.0	18.0
2150-2199	12.0	38.0	28.000000000000004	22.0
2200-2249	18.0	30.0	34.0	18.0
2250-2299	18.0	34.0	34.0	14.000000000000002
2300-2327	17.857142857142858	46.42857142857143	21.428571428571427	14.285714285714285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.5
9	0.5
10	0.2
11	0.6004329004329004
12	0.7746031746031745
13	1.926695526695527
14	3.8045815295815295
15	5.912806637806638
16	7.629184704184705
17	10.920382395382392
18	11.980772005772007
19	17.522005772005766
20	19.765187590187587
21	19.94209956709956
22	29.988492063492032
23	30.344047619047586
24	29.227020202020164
25	28.77896825396823
26	20.09444444444444
27	20.909523809523805
28	14.575000000000008
29	10.900000000000002
30	9.033333333333331
31	9.483333333333334
32	13.333766233766239
33	10.802380952380954
34	13.488095238095235
35	19.560389610389606
36	25.01273448773448
37	30.358441558441545
38	30.892496392496383
39	35.325901875901856
40	41.640115440115466
41	34.70916305916305
42	37.149603174603165
43	36.74444444444442
44	29.899999999999984
45	24.8595238095238
46	17.59285714285715
47	17.308333333333334
48	14.416666666666668
49	9.183333333333332
50	5.999999999999999
51	6.625
52	4.75
53	2.4166666666666665
54	2.833333333333333
55	1.5
56	0.5
57	0.0
58	0.2
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.3333333333333333
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-49	0.0
50-99	0.0
100-149	0.0
150-199	0.0
200-249	0.0
250-299	0.0
300-349	0.0
350-399	0.0
400-449	0.0
450-499	0.0
500-549	0.0
550-599	0.0
600-649	0.0
650-699	0.0
700-749	0.0
750-799	0.0
800-849	0.0
850-899	0.0
900-949	0.0
950-999	0.0
1000-1049	0.0
1050-1099	0.0
1100-1149	0.0
1150-1199	0.0
1200-1249	0.0
1250-1299	0.0
1300-1349	0.0
1350-1399	0.0
1400-1449	0.0
1450-1499	0.0
1500-1549	0.0
1550-1599	0.0
1600-1649	0.0
1650-1699	0.0
1700-1749	0.0
1750-1799	0.0
1800-1849	0.0
1850-1899	0.0
1900-1949	0.0
1950-1999	0.0
2000-2049	0.0
2050-2099	0.0
2100-2149	0.0
2150-2199	0.0
2200-2249	0.0
2250-2299	0.0
2300-2327	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
100-149	7.0
150-199	142.0
200-249	402.0
250-299	346.0
300-349	206.0
350-399	170.0
400-449	131.0
450-499	98.0
500-549	77.0
550-599	62.0
600-649	41.0
650-699	29.0
700-749	35.0
750-799	27.0
800-849	24.0
850-899	9.0
900-949	14.0
950-999	5.0
1000-1049	8.0
1050-1099	7.0
1100-1149	5.0
1150-1199	5.0
1200-1249	5.0
1250-1299	1.0
1300-1349	3.0
1350-1399	7.0
1400-1449	5.0
1450-1499	1.0
1500-1549	0.0
1550-1599	0.0
1600-1649	1.0
1650-1699	1.0
1700-1749	0.0
1750-1799	0.0
1800-1849	0.0
1850-1899	0.0
1900-1949	1.0
1950-1999	0.0
2000-2049	0.0
2050-2099	0.0
2100-2149	0.0
2150-2199	0.0
2200-2249	0.0
2250-2299	0.0
2300-2328	1.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	100.0
#Duplication Level	Percentage of total
1	100.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
>10	0.0
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10-49	0.0	0.0	0.0	0.0	0.0	0.0
50-99	0.0	0.0	0.0	0.0	0.003198294243070362	0.0
100-149	0.0	0.0	0.0	0.0	0.10127931769722809	0.0
150-199	0.0	0.0	0.0	0.0	0.2505330490405117	0.0
200-249	0.0	0.0	0.0	0.0	0.3923240938166311	0.0
250-299	0.0	0.0	0.0	0.0	0.5415778251599147	0.0
300-349	0.0	0.0	0.0	0.0	0.7142857142857144	0.0
350-399	0.0	0.0	0.0	0.0	0.835820895522388	0.0
400-449	0.0	0.0	0.0	0.0	0.8869936034115148	0.0
450-499	0.0	0.0	0.0	0.0	0.906183368869937	0.0
500-549	0.0	0.0	0.0	0.0	0.906183368869937	0.0
550-599	0.0	0.0	0.0	0.0	0.9125799573560771	0.0
600-649	0.0	0.0	0.0	0.0	0.9840085287846481	0.0
650-699	0.0	0.0	0.0	0.0	1.061833688699361	0.0
700-749	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
750-799	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
800-849	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
850-899	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
900-949	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
950-999	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1000-1049	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1050-1099	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1100-1149	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1150-1199	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1200-1249	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1250-1299	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1300-1349	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1350-1399	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1400-1449	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1450-1499	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1500-1549	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1550-1599	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1600-1649	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1650-1699	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1700-1749	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1750-1799	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1800-1849	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1850-1899	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1900-1949	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
1950-1999	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
2000-2049	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
2050-2099	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
2100-2149	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
2150-2199	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
2200-2249	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
2250-2299	0.0	0.0	0.0	0.0	1.0660980810234546	0.0
2300-2316	0.0	0.0	0.0	0.0	1.0660980810234544	0.0
>>END_MODULE