##FastQC 0.12.1 >>Basic Statistics pass #Measure Value Filename sample2_2_fastqsanger File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 339276 Total Bases 16.9 Mbp Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.501049293200815 40.0 39.0 40.0 36.0 40.0 2 38.42000023579622 40.0 39.0 40.0 36.0 40.0 3 38.39282766832903 40.0 39.0 40.0 36.0 40.0 4 38.330291562032095 40.0 39.0 40.0 36.0 40.0 5 38.32326483453 40.0 39.0 40.0 36.0 40.0 6 38.3043215553119 40.0 39.0 40.0 36.0 40.0 7 38.27235053466794 40.0 39.0 40.0 35.0 40.0 8 38.21486046758391 40.0 39.0 40.0 35.0 40.0 9 38.16393732536342 40.0 39.0 40.0 35.0 40.0 10 38.07507751800894 40.0 39.0 40.0 35.0 40.0 11 37.995643664744925 40.0 39.0 40.0 35.0 40.0 12 37.94393060517101 40.0 38.0 40.0 35.0 40.0 13 37.87864452540115 40.0 38.0 40.0 35.0 40.0 14 37.81735519164338 40.0 38.0 40.0 35.0 40.0 15 37.70528419340006 40.0 38.0 40.0 35.0 40.0 16 37.54292080783787 40.0 38.0 40.0 34.0 40.0 17 37.49317075183626 40.0 38.0 40.0 33.0 40.0 18 37.37134957969323 40.0 38.0 40.0 33.0 40.0 19 37.29874497459296 40.0 38.0 40.0 33.0 40.0 20 37.21135005128568 40.0 38.0 40.0 33.0 40.0 21 37.13023909737205 40.0 38.0 40.0 33.0 40.0 22 37.21558554097549 40.0 38.0 40.0 33.0 40.0 23 37.149285537438544 40.0 38.0 40.0 33.0 40.0 24 37.11127223853146 40.0 38.0 40.0 33.0 40.0 25 37.04431200556479 40.0 38.0 40.0 33.0 40.0 26 37.021828835520346 40.0 38.0 40.0 33.0 40.0 27 36.91379290017567 40.0 38.0 40.0 33.0 40.0 28 36.85172838632853 40.0 38.0 40.0 33.0 40.0 29 36.979134981548945 40.0 38.0 40.0 33.0 40.0 30 36.9414459024511 40.0 38.0 40.0 33.0 40.0 31 36.9950630165411 40.0 38.0 40.0 33.0 40.0 32 36.9632629481602 40.0 38.0 40.0 33.0 40.0 33 36.85937407892099 40.0 38.0 40.0 33.0 40.0 34 36.805252950400266 40.0 38.0 40.0 33.0 40.0 35 36.72665912118747 40.0 38.0 40.0 32.0 40.0 36 36.723611454980606 40.0 38.0 40.0 33.0 40.0 37 36.617983588582746 40.0 38.0 40.0 32.0 40.0 38 36.55384406795647 40.0 38.0 40.0 32.0 40.0 39 36.42572713660854 40.0 37.0 40.0 31.0 40.0 40 36.41227790943067 40.0 38.0 40.0 31.0 40.0 41 36.29241974085995 40.0 37.0 40.0 31.0 40.0 42 36.15780072861033 40.0 37.0 40.0 31.0 40.0 43 36.05285077635907 40.0 37.0 40.0 30.0 40.0 44 35.964975418243554 40.0 36.0 40.0 30.0 40.0 45 35.86212110494111 39.0 36.0 40.0 30.0 40.0 46 35.73081797710419 39.0 36.0 40.0 29.0 40.0 47 35.60673021374928 39.0 36.0 40.0 29.0 40.0 48 35.52411311144908 39.0 36.0 40.0 29.0 40.0 49 35.447824190334714 39.0 36.0 40.0 29.0 40.0 50 35.34864535068794 39.0 36.0 40.0 29.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1 1 0.010457816165470035 1 2 -0.5118376974797769 1 3 -0.36419102831982997 1 4 -0.544946702404232 1 5 -0.4981649462798359 1 6 -0.4954273135275997 1 7 -0.6298412378212177 1 8 -0.5248464105557744 1 9 -0.5900102191618899 1 10 -0.6418305129846757 1 11 -0.7194242373357085 1 12 -0.6163129234501952 1 13 -0.7153853272073007 1 14 -0.7195245931988126 1 15 -0.8529517300232072 1 16 -0.6630684138337273 1 17 -0.616704693567911 1 18 -0.7043277617759287 1 19 -1.024744580210779 1 20 -1.0448164926241077 1 21 -0.9579511747905727 1 22 -0.9467992941924095 1 23 -0.917079825392932 1 24 -0.8144488599247595 1 25 -0.8545192539651794 1 26 -0.6862316340117331 1 27 -0.853518619550151 1 28 -0.8910270830564428 1 29 -0.9751231623183116 1 30 -0.8198100819817569 1 31 -0.7495976285494663 1 32 -0.5557319882199039 1 33 -0.6103438568917525 1 34 -0.47702383551362715 1 35 -0.7167868557362738 1 36 -0.640955111537096 1 37 -0.6741427022865736 1 38 -0.7866766595763295 1 39 -0.737036864176126 1 40 -0.8681219147260109 1 41 -0.4069953244678217 1 42 -0.5798589371163914 1 43 -0.7702408141090871 1 44 -0.8496463265251677 1 45 -0.7482207744813962 1 46 -0.8062808787964642 1 47 -0.7987017159321823 1 48 -0.7399586176150237 1 49 -0.6805929964967916 1 50 -1.1460873107401994 2 1 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-0.27408918803092774 120 39 -0.6617368613657959 120 40 -0.6093410362166125 120 41 -0.5833435806575764 120 42 -0.6474080476466995 120 43 -0.8570350697929783 120 44 -0.5480303863290104 120 45 -0.7513683449504427 120 46 -0.8618130149288845 120 47 -0.8251075839028488 120 48 -0.6881642214145884 120 49 -1.0115375486705815 120 50 -0.6926797846743824 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3885.0 3 242.0 4 330.0 5 370.0 6 327.0 7 572.0 8 712.0 9 533.0 10 427.0 11 606.0 12 686.0 13 567.0 14 511.0 15 583.0 16 594.0 17 444.0 18 511.0 19 555.0 20 652.0 21 672.0 22 777.0 23 887.0 24 1047.0 25 1086.0 26 1269.0 27 1483.0 28 1672.0 29 1944.0 30 2439.0 31 2921.0 32 3513.0 33 4730.0 34 6310.0 35 8925.0 36 13583.0 37 22678.0 38 51085.0 39 198483.0 40 665.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.23524745911198 11.302142218696911 10.923006801351983 43.53960352083913 2 29.83650218243693 17.015314048975362 27.916549530221204 25.231634238366503 3 28.422526124515763 19.79011603990518 21.345009337054343 30.44234849852472 4 29.922273658288244 24.65221457916484 19.082320069142703 26.34319169340421 5 30.135096734471574 26.44243292524095 22.477444410239407 20.94502593004807 6 26.388206533641924 29.614184833758532 22.135834403726744 21.8617742288728 7 22.8733118057426 19.537916480446103 36.141786119143674 21.44698559466763 8 23.7776705667157 23.273910122269942 27.694689204668222 25.25373010634614 9 23.08159013152443 21.44256608776977 30.009707473570813 25.466136307134995 10 23.628382238456506 31.822716091773696 24.7348526334173 19.814049036352497 11 28.155756508354667 25.71640001776015 20.94217591427768 25.1856675596075 12 25.40517499119346 22.849945384631322 25.784078361953366 25.960801262221857 13 24.376330302931628 24.781304858806568 27.378411422177095 23.463953416084713 14 25.075792565223054 25.12967634206132 26.44479577456449 23.349735318151136 15 25.003035535009342 25.566016223083686 25.5405473439809 23.890400897926064 16 25.135663507109008 25.597156398104264 25.187203791469194 24.079976303317537 17 25.249162547277948 25.347494750185856 25.21924823253789 24.184094469998314 18 24.782491490729413 25.278976695411064 25.41909453658912 24.5194372772704 19 25.280627586676818 24.78825171013222 25.192343584745235 24.73877711844573 20 25.165013568440635 25.38276037778331 25.185455105644234 24.26677094813182 21 25.012146708538246 25.0154055815607 25.57741304734254 24.395034662558512 22 25.127039671933936 25.205559819018468 25.15992924297689 24.507471266070706 23 25.13444882648027 25.280823967595982 25.498905149501166 24.08582205642258 24 24.83337432912608 25.348143186943776 25.461943556202264 24.356538927727875 25 25.329381252315674 25.20370507595406 25.023786587625047 24.443127084105225 26 25.203717152554432 25.414474366764985 25.24758785255888 24.13422062812171 27 24.698995168509352 25.500489077274207 25.33390639357382 24.466609360642618 28 24.92093180423693 25.46662833057175 25.09314781825134 24.51929204693998 29 25.305278254350643 25.239200068745017 25.09519110818747 24.36033056871687 30 24.782222222222224 25.696296296296296 25.28948148148148 24.232 31 25.099490619879163 25.022742829711763 25.141568558889855 24.73619799151922 32 24.85757707724166 25.353502137454786 25.150273588287398 24.638647197016155 33 24.783774574438137 25.39000577786338 25.22289219099542 24.603327456703063 34 25.122584965083654 25.118733352887674 25.196950708252224 24.561730973776445 35 25.14417121554263 25.36524362416505 24.979107768354048 24.511477391938268 36 25.046375591918498 25.358410724763086 25.210838553674193 24.384375129644226 37 24.952510779534443 25.110166101142404 25.29804857087822 24.63927454844493 38 25.365372306379346 25.369817224948438 24.972145176967025 24.29266529170519 39 24.9867375577546 25.330820137573756 25.26087742539691 24.42156487927473 40 25.28131677291061 25.336439153486197 24.648891180715466 24.73335289288773 41 25.57082903581463 25.377885265142087 24.6398975708646 24.411388128178682 42 25.145895512434834 25.378261346358467 25.05194146989173 24.423901671314972 43 25.53468923272058 25.12098482985073 24.503095374275055 24.841230563153637 44 25.574564445471953 25.460155430278668 24.732205841380495 24.23307428286888 45 24.912495961922033 25.67624839588279 24.889082519049353 24.52217312314582 46 25.237445900278647 25.415604434694966 24.668583624829548 24.678366040196835 47 25.606492170804795 25.25645319525417 24.912638452122316 24.224416181818718 48 25.046017043349387 25.51226380140793 25.01756206002223 24.42415709522045 49 25.670752493737865 25.168003082897332 24.686893239858303 24.4743511835065 50 25.704520730264974 25.245066798673214 24.846675776699463 24.20373669436235 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1289.0 1 668.0 2 47.0 3 49.0 4 51.0 5 49.0 6 47.0 7 50.5 8 54.0 9 63.0 10 72.0 11 75.5 12 79.0 13 99.5 14 120.0 15 175.0 16 230.0 17 326.5 18 423.0 19 559.0 20 695.0 21 954.0 22 1213.0 23 1556.0 24 1899.0 25 2448.0 26 2997.0 27 3668.0 28 4339.0 29 5085.5 30 5832.0 31 6778.5 32 7725.0 33 9098.0 34 10471.0 35 11674.5 36 12878.0 37 13723.0 38 14568.0 39 15649.5 40 16731.0 41 17656.5 42 18582.0 43 19036.0 44 19490.0 45 19902.0 46 20314.0 47 20174.0 48 20034.0 49 19928.0 50 19822.0 51 20064.5 52 20307.0 53 19994.5 54 19682.0 55 19239.5 56 18797.0 57 18201.0 58 17605.0 59 16831.0 60 16057.0 61 15097.0 62 14137.0 63 13087.0 64 12037.0 65 10966.0 66 9895.0 67 8890.5 68 7886.0 69 6920.5 70 5955.0 71 5306.5 72 4658.0 73 4007.0 74 3356.0 75 2889.0 76 2422.0 77 2062.0 78 1702.0 79 1496.5 80 1291.0 81 1183.0 82 1075.0 83 930.5 84 786.0 85 689.0 86 592.0 87 483.0 88 374.0 89 326.0 90 278.0 91 231.0 92 184.0 93 140.5 94 97.0 95 80.0 96 63.0 97 44.0 98 25.0 99 19.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4132328841415249 2 0.39820087480399435 3 0.4052747615510676 4 0.41971728032634203 5 0.42561218594890293 6 0.4108749218925005 7 0.4170645727961895 8 0.4176540633584456 9 0.4102854313302444 10 0.42590693123003104 11 0.42472795010551884 12 0.4300333651658237 13 0.43533878022612854 14 0.4456548650656103 15 0.4742451573350311 16 0.49399309117061035 17 0.48485598745564085 18 0.5019512137610677 19 0.509614591070397 20 0.5093198457892689 21 0.5116778080382933 22 0.5255308362513116 23 0.5267098173758238 24 0.5432155531189945 25 0.5603107794244214 26 0.5662056850469824 27 0.5617845058300617 28 0.5626687416734457 29 0.5296572701871043 30 0.5234676192834153 31 0.5326047229983848 32 0.5084356099458848 33 0.5249413456890556 34 0.5172779683797263 35 0.53938386446433 36 0.5346679399662811 37 0.539678609745458 38 0.534078449404025 39 0.5473419870547873 40 0.5438050436812507 41 0.5517631662717081 42 0.5532368926773482 43 0.5414470814322263 44 0.5570685813320129 45 0.5491104587415555 46 0.5706268642639031 47 0.5555948549263726 48 0.5603107794244214 49 0.5691531378582628 50 0.5656161944847262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 339276.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.50464245749873 #Duplication Level Percentage of total 1 87.52735123717265 2 5.127159620191729 3 1.9037366823053754 4 1.207471840729403 5 0.7692731492262534 6 0.5453789849491104 7 0.439186897709665 8 0.3106519259663469 9 0.28316117150295633 >10 1.4205361170950987 >50 0.01648842254989314 >100 0.0903918879074499 >500 0.0 >1k 0.35921206269410055 >5k 0.0 >10k+ 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1220 0.35958924297621997 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence PolyA PolyG 1 2.9474528112804913E-4 0.0 0.0 0.0 0.05482262228981714 0.0011789811245121965 2 8.842358433841474E-4 0.0 0.0 0.0 0.05924380150673788 0.002357962249024393 3 0.0011789811245121965 2.9474528112804913E-4 0.0 0.0 0.06219125431801837 0.002652707530152442 4 0.002063216967896344 2.9474528112804913E-4 0.0 0.0 0.066022942972683 0.0029474528112804913 5 0.0032421980924085404 2.9474528112804913E-4 0.0 0.0 0.0683809052217074 0.0035369433735365895 6 0.0032421980924085404 2.9474528112804913E-4 0.0 0.0 0.0701493769084757 0.004126433935792688 7 0.0032421980924085404 2.9474528112804913E-4 0.0 0.0 0.07162310331411594 0.005600160341432934 8 0.004421179216920737 2.9474528112804913E-4 0.0 0.0 0.07722326365554888 0.00677914146594513 9 0.004421179216920737 2.9474528112804913E-4 0.0 0.0 0.0857708768082623 0.007663377309329277 10 0.005600160341432934 2.9474528112804913E-4 0.0 0.0 0.09726594277225621 0.008547613152713424 11 0.006189650903689032 2.9474528112804913E-4 0.0 0.0 0.10286610311368916 0.00972659427722562 12 0.006484396184817081 2.9474528112804913E-4 0.0 0.0 0.10905575401737819 0.010610830120609769 13 0.007368632028201229 2.9474528112804913E-4 0.0 0.0 0.11406642379655502 0.011789811245121965 14 0.007958122590457327 2.9474528112804913E-4 0.0 0.0 0.12025607470024405 0.01385302821301831 15 0.01709522630542685 2.9474528112804913E-4 0.0 0.0 0.12791945200957333 0.015326754618658555 16 0.018568952711067097 2.9474528112804913E-4 0.0 0.0 0.1373513010056709 0.0173899715865549 17 0.019747933835579292 2.9474528112804913E-4 0.0 0.0 0.14383569719048797 0.019747933835579292 18 0.02357962249024393 2.9474528112804913E-4 0.0 0.0 0.14825687640740873 0.021221660241219537 19 0.02770605642603662 2.9474528112804913E-4 0.0 0.0 0.1529728009054575 0.022695386646859782 20 0.18392105542390266 2.9474528112804913E-4 0.0 0.0 0.15916245180914654 0.024463858333628078 21 0.19482663082564047 2.9474528112804913E-4 0.0 0.0 0.16417312158832337 0.02741131114490857 22 0.2004267911670734 2.9474528112804913E-4 0.0 0.0 0.1697732819297563 0.03065350923731711 23 0.21516405522347587 2.9474528112804913E-4 0.0 0.0 0.17478395170893313 0.0336009620485976 24 0.22754335703085393 2.9474528112804913E-4 0.0 0.0 0.18038411205036609 0.03625366957875004 25 0.24139638524387225 2.9474528112804913E-4 0.0 0.0 0.1842158007050307 0.03772739598439029 26 0.264091771890732 2.9474528112804913E-4 0.0 0.0 0.19040545160871974 0.039790612952286634 27 0.2973979886582016 2.9474528112804913E-4 0.0 0.0 0.19600561195015268 0.042148575201311024 28 0.3551680637592992 2.9474528112804913E-4 0.0 0.0 0.20190051757271366 0.043327556325823226 29 0.40881170492460417 2.9474528112804913E-4 0.0 0.0 0.20661644207076243 0.04480128273146347 30 0.4763083743029274 2.9474528112804913E-4 0.0 0.0 0.21339558353670757 0.046275009137103716 31 0.5502894398660677 2.9474528112804913E-4 0.0 0.0 0.2189957438781405 0.04804348082387201 32 0.6384182789233545 2.9474528112804913E-4 0.0 0.0 0.22577488534408563 0.05010669779176835 33 0.7471792876596046 2.9474528112804913E-4 0.0 0.0 0.23196453624777466 0.05482262228981714 34 0.8594772397693913 2.9474528112804913E-4 0.0 0.0 0.23668046074582347 0.05806482038222568 35 1.0086183520201841 2.9474528112804913E-4 0.0 0.0 0.2387436777137198 0.061012273193506175 36 1.1542225208974404 2.9474528112804913E-4 0.0 0.0 0.2440490927740247 0.06337023544253056 37 1.3063110859595137 2.9474528112804913E-4 0.0 0.0 0.24758603614756128 0.07221259387637204 38 1.4684209905799408 2.9474528112804913E-4 0.0 0.0 0.2525967059267381 0.07516004668765253 39 1.6399627441964655 2.9474528112804913E-4 0.0 0.0 0.2567231398625308 0.08724460321390255 >>END_MODULE