##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Trimmomatic on SRR13615650_reverse _R1 paired_.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13602338
Total Bases	858.1 Mbp
Sequences flagged as poor quality	0
Sequence length	36-64
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.25593181113423	36.0	36.0	36.0	36.0	36.0
2	35.25359941798241	36.0	36.0	36.0	36.0	36.0
3	35.257013389904	36.0	36.0	36.0	36.0	36.0
4	35.25675152315727	36.0	36.0	36.0	36.0	36.0
5	35.25219010143697	36.0	36.0	36.0	36.0	36.0
6	35.25186508378192	36.0	36.0	36.0	36.0	36.0
7	35.245497060872914	36.0	36.0	36.0	36.0	36.0
8	35.24837884487211	36.0	36.0	36.0	36.0	36.0
9	35.2550985720249	36.0	36.0	36.0	36.0	36.0
10	35.244251980799184	36.0	36.0	36.0	36.0	36.0
11	35.222743840066315	36.0	36.0	36.0	36.0	36.0
12	35.22114352694368	36.0	36.0	36.0	36.0	36.0
13	35.20874970170569	36.0	36.0	36.0	36.0	36.0
14	35.204178281704216	36.0	36.0	36.0	36.0	36.0
15	35.200117656244096	36.0	36.0	36.0	36.0	36.0
16	35.20276080479694	36.0	36.0	36.0	36.0	36.0
17	35.196403956437486	36.0	36.0	36.0	36.0	36.0
18	35.19916789304897	36.0	36.0	36.0	36.0	36.0
19	35.186881696367195	36.0	36.0	36.0	36.0	36.0
20	35.18364747295649	36.0	36.0	36.0	36.0	36.0
21	35.18569851741664	36.0	36.0	36.0	36.0	36.0
22	35.1818217574067	36.0	36.0	36.0	36.0	36.0
23	35.17203549860326	36.0	36.0	36.0	36.0	36.0
24	35.16976118370239	36.0	36.0	36.0	36.0	36.0
25	35.149599355640184	36.0	36.0	36.0	36.0	36.0
26	35.13007977011011	36.0	36.0	36.0	36.0	36.0
27	35.10078039525264	36.0	36.0	36.0	36.0	36.0
28	35.095498141569486	36.0	36.0	36.0	36.0	36.0
29	35.082176534651616	36.0	36.0	36.0	36.0	36.0
30	35.04889924070406	36.0	36.0	36.0	36.0	36.0
31	35.035959921007695	36.0	36.0	36.0	36.0	36.0
32	35.01644099712858	36.0	36.0	36.0	36.0	36.0
33	35.003792215720566	36.0	36.0	36.0	36.0	36.0
34	34.99067851423777	36.0	36.0	36.0	36.0	36.0
35	34.97649926064181	36.0	36.0	36.0	36.0	36.0
36	34.955246737729944	36.0	36.0	36.0	36.0	36.0
37	34.93205682183831	36.0	36.0	36.0	36.0	36.0
38	34.91026288991195	36.0	36.0	36.0	32.0	36.0
39	34.872015894995805	36.0	36.0	36.0	32.0	36.0
40	34.847550193225906	36.0	36.0	36.0	32.0	36.0
41	34.84915040549801	36.0	36.0	36.0	32.0	36.0
42	34.81529391485448	36.0	36.0	36.0	32.0	36.0
43	34.80386126299538	36.0	36.0	36.0	32.0	36.0
44	34.796083821891486	36.0	36.0	36.0	32.0	36.0
45	34.76017579404086	36.0	36.0	36.0	32.0	36.0
46	34.708319115206294	36.0	36.0	36.0	32.0	36.0
47	34.69448795616861	36.0	36.0	36.0	32.0	36.0
48	34.66375823911571	36.0	36.0	36.0	32.0	36.0
49	34.64898529738607	36.0	36.0	36.0	32.0	36.0
50	34.64506546864858	36.0	36.0	36.0	32.0	36.0
51	34.630021500172035	36.0	36.0	36.0	32.0	36.0
52	34.611952222253166	36.0	36.0	36.0	32.0	36.0
53	34.60264964813595	36.0	36.0	36.0	32.0	36.0
54	34.59398488041586	36.0	36.0	36.0	32.0	36.0
55	34.56363666003541	36.0	36.0	36.0	32.0	36.0
56	34.557615111206054	36.0	36.0	36.0	32.0	36.0
57	34.53579593005158	36.0	36.0	36.0	32.0	36.0
58	34.53186168174002	36.0	36.0	36.0	32.0	36.0
59	34.51802725417532	36.0	36.0	36.0	32.0	36.0
60	34.5342800257606	36.0	36.0	36.0	32.0	36.0
61	34.53152611394628	36.0	36.0	36.0	32.0	36.0
62	34.53827062642353	36.0	36.0	36.0	32.0	36.0
63	34.671905293975584	36.0	36.0	36.0	32.0	36.0
64	34.79981862439703	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	20.0
21	135.0
22	734.0
23	2952.0
24	9152.0
25	23029.0
26	47815.0
27	85250.0
28	132691.0
29	189493.0
30	255256.0
31	336938.0
32	458734.0
33	693891.0
34	1397292.0
35	7510816.0
36	2458139.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	21.958295824178894	28.33438034264222	31.68269038139688	18.024633451782005
2	22.174885873115237	27.552021972940377	30.968693632677397	19.30439852126699
3	22.695170973052743	27.297712532132667	30.166475986755675	19.840640508058915
4	22.489104671610978	26.840670893446333	30.1888303034224	20.48139413152029
5	22.4342424388123	26.565460696615727	29.652778727381722	21.34751813719025
6	22.723344546487738	26.5362851054967	29.318258493731044	21.422111854284523
7	22.485538058618815	26.334231076702437	29.47185811998511	21.708372744693637
8	22.472988833828534	26.098030141347987	29.32114514920147	22.107835875622005
9	22.562410781219985	26.318721297164693	29.069826512779613	22.049041408835713
10	22.41739899616652	26.23864011969779	29.183710408038195	22.160250476097495
11	22.40775680632745	26.189528514366355	28.979997053416962	22.422717625889234
12	22.759240312262516	26.159642583265313	28.829100842190126	22.252016262282044
13	22.380418504906917	26.43801788383228	28.996083730388328	22.185479880872474
14	22.444102850857348	26.251639525464114	28.847671552203202	22.456586071475336
15	22.576613770025915	26.487179502260087	28.511216336507445	22.42499039120655
16	22.559327028093627	26.566345876176793	28.736710269314624	22.13761682641496
17	22.670449662412988	26.349158901480497	28.46080628742068	22.519585148685834
18	22.65366441513651	26.778142613704574	28.31370789206447	22.25448507909444
19	22.589283948551532	26.83766507356571	28.40961747859334	22.163433499289418
20	22.712810165704752	26.658119032906797	28.084968576785897	22.544102224602554
21	22.736414946139703	27.003914358735937	27.922638850047214	22.337031845077146
22	22.966554383120165	26.768888584035594	28.010109562657316	22.25444747018693
23	22.853213251115477	26.80400487096386	27.79876043909251	22.544021438828146
24	23.26109697486861	26.888691812244726	27.630721401236997	22.21948981164967
25	22.999837158754776	26.865416305665146	27.826091431499528	22.30865510408055
26	23.075469131945084	26.76524104468011	27.530908489404325	22.62838133397048
27	23.36928469160263	26.838685724893264	27.637116858149525	22.154912725354585
28	23.085563083260514	26.877560523674187	27.85965915214585	22.177217240919443
29	23.44078816827158	26.70427949799273	27.512850242014697	22.342082091720993
30	23.35354879187694	26.860026935810094	27.621747648704044	22.164676623608923
31	23.525120432878314	26.86867164511036	27.48747884038899	22.118729081622337
32	23.683136589265157	26.566591597867077	27.449081908801592	22.301189904066177
33	23.74913093121754	26.89712100311986	27.312936000146447	22.040812065516153
34	23.692046513279482	27.029302489747447	27.365696033655674	21.912954963317393
35	23.72097270189506	26.93221433401021	27.285151108063555	22.061661856031172
36	23.842470897138952	27.097639092542853	27.214973182617353	21.844916827700843
37	23.76157065889756	27.204333420361326	27.36407356344771	21.670022357293398
38	24.109631211800796	27.027080744024822	27.0772180012997	21.78607004287468
39	24.202765926009512	27.31693333083883	27.014557947253277	21.465742795898386
40	24.304612210859297	27.166750012253093	27.188492339146396	21.340145437741214
41	24.200962676733365	27.133853777799672	27.189629380962337	21.475554164504622
42	24.54099403040429	27.334002418417164	26.926382463664154	21.198621087514397
43	24.324088052144088	27.36382563644711	27.206675102567967	21.10541120884083
44	24.510364605777948	27.56609905129668	26.70595457190661	21.21758177101876
45	24.833269807212762	27.561711757398626	26.648828098317733	20.956190337070876
46	24.507645499729627	27.78442879007221	26.701831058534253	21.00609465166392
47	24.687772749582294	27.386149373679974	26.67163892933736	21.25443894740037
48	24.673675848847534	27.59691282075155	26.630834995167575	21.098576335233343
49	24.8104403262889	27.13682451134689	27.14498106285831	20.9077540995059
50	24.620207179900692	27.00082315192793	27.049030336628345	21.329939331543038
51	24.983688583622893	26.78813972756446	27.149484088514132	21.078687600298522
52	25.114790905602334	26.814164509867172	26.998515945898387	21.072528638632104
53	25.318303485722115	26.757095622151034	27.036412927938642	20.88818796418821
54	25.15911174648895	27.07015753477835	27.04189728273837	20.728833435994332
55	24.735027651320856	27.01805117968642	27.323154816415148	20.923766352577577
56	25.068087185802728	26.344170948627816	27.437071923731782	21.150669941837677
57	25.39214569034919	26.084265264897056	27.204374252025538	21.319214792728218
58	24.78375831838759	26.453439180026255	27.495664991209445	21.267137510376706
59	25.281689727162828	25.931558280180816	27.17677416221885	21.609977830437504
60	25.09786107019851	26.597088590054447	27.112328770558975	21.192721569188073
61	25.015488754015525	26.558387484820077	27.825245598421215	20.600878162743175
62	24.427767174687524	26.29415471834372	27.827625663911437	21.45045244305732
63	25.266535277199935	26.468689451497195	27.11812806073982	21.146647210563046
64	26.057969203447062	25.465524841470383	27.847050794364808	20.629455160717743
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10887.0
1	6330.0
2	1745.5
3	1600.5
4	1401.5
5	1357.5
6	1412.5
7	1501.5
8	1617.5
9	1883.0
10	2446.5
11	2869.0
12	3738.5
13	5869.5
14	7349.5
15	10118.0
16	16935.5
17	21680.5
18	28788.5
19	35777.5
20	46492.0
21	70663.0
22	85160.5
23	104001.5
24	142093.0
25	163913.5
26	189809.0
27	239886.5
28	269031.0
29	301025.5
30	359093.0
31	391006.5
32	419600.0
33	447761.0
34	475731.0
35	524869.0
36	551746.5
37	580093.5
38	627311.0
39	653120.0
40	672589.0
41	695944.5
42	707515.5
43	719261.5
44	731035.5
45	740851.0
46	746828.5
47	749643.0
48	747398.5
49	731895.5
50	720196.5
51	694475.5
52	639371.0
53	602325.0
54	563595.5
55	502057.5
56	465544.5
57	424744.0
58	384929.0
59	351345.5
60	303725.5
61	280693.5
62	258913.5
63	209344.0
64	176006.5
65	158197.0
66	133443.0
67	122550.0
68	105604.5
69	88673.0
70	75126.0
71	53708.0
72	43435.5
73	37018.5
74	27982.0
75	23757.0
76	22146.0
77	18469.5
78	15708.5
79	13380.0
80	10535.0
81	9705.5
82	6236.5
83	2725.5
84	1964.0
85	1054.5
86	732.0
87	577.5
88	324.0
89	215.5
90	146.0
91	68.5
92	56.5
93	47.5
94	41.5
95	49.0
96	70.5
97	95.0
98	161.0
99	12910.5
100	25604.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.57222765674548E-4
2	0.0011248066325068528
3	9.998281177838692E-4
4	0.0013085985659230053
5	0.001293895211249713
6	0.0011689166965267294
7	6.175408962782722E-4
8	6.83705992308087E-4
9	0.0017717542381317094
10	6.0283754160498E-4
11	0.0010439381818037458
12	4.7785902688199627E-4
13	7.939811523577785E-4
14	5.807825095950417E-4
15	0.001573258950042265
16	7.792777976844863E-4
17	4.925623815552885E-4
18	0.0013306535979329436
19	0.001065993213813684
20	9.77773085773931E-4
21	8.086845070310707E-4
22	0.0012277301152198983
23	7.498710883379019E-4
24	0.001360060307279528
25	9.77773085773931E-4
26	6.469476056248565E-4
27	0.0011983234058733137
28	0.0010292348271304535
29	4.7785902688199627E-4
30	0.002727472291895702
31	0.0026686588732025333
32	0.0010953999231602684
33	9.924764404472231E-4
34	8.454428937143012E-4
35	0.001360060307279528
36	0.0011248066325068528
37	0.0013168052450927671
38	0.0016047198196000479
39	7.218302078576374E-4
40	0.0022847346925539387
41	0.0031268169092358794
42	8.338282189901499E-4
43	3.9132426716224564E-4
44	8.4960210547655E-4
45	0.0013084533999496283
46	9.690394843644739E-4
47	0.0016434106782607562
48	8.005798570253329E-4
49	8.797665546902779E-4
50	9.97431800600634E-4
51	9.052386386327337E-4
52	0.0013507085035219723
53	5.72509032284166E-4
54	7.372185313086343E-4
55	7.416999716995104E-4
56	9.639469835378067E-4
57	4.4582031720506634E-4
58	6.966021391321104E-4
59	6.265867820735089E-4
60	9.013232130150445E-4
61	2.5098816786873004E-4
62	2.2007848313106284E-4
63	3.011935269072426E-4
64	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
36	8832.0
37	8580.0
38	8326.0
39	8285.0
40	8199.0
41	8164.0
42	8197.0
43	8007.0
44	8327.0
45	8880.0
46	10049.0
47	18270.0
48	77573.0
49	78404.0
50	78072.0
51	77904.0
52	78039.0
53	78311.0
54	78680.0
55	79461.0
56	78358.0
57	9117.0
58	8718.0
59	8566.0
60	9414.0
61	26871.0
62	106261.0
63	980950.0
64	1.1635523E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.56483598185372
#Duplication Level	Percentage of total
1	60.298719087040695
2	15.587051144146086
3	5.064675749360213
4	2.5172588394446223
5	1.5861894924162976
6	1.1819453509679394
7	0.8849358213472847
8	0.6904833772400158
9	0.5421889375723987
>10	6.042844561566382
>50	1.618310667274305
>100	2.289633944446602
>500	0.7528647009320529
>1k	0.7233150991856171
>5k	0.04025791367375122
>10k+	0.1793253133859506
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	24183	0.17778561303211257	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.00378611382837274	2.205503200993829E-5	0.0	1.4703354673292195E-5	0.01554144588966985	0.18976149541350906
2	0.006087188834742969	3.675838668323049E-5	0.0	2.205503200993829E-5	0.01959222010216185	0.189783550445519
3	0.009226355057490853	4.411006401987658E-5	0.0	3.675838668323049E-5	0.023348927221188004	0.18981295715486557
4	0.011703870319940586	4.411006401987658E-5	0.0	4.411006401987658E-5	0.02603228944906383	0.18984236386421216
5	0.015379708988263635	4.411006401987658E-5	0.0	4.411006401987658E-5	0.029318489218544637	0.1898497155415488
6	0.01887175572317053	4.411006401987658E-5	0.0	5.881341869316878E-5	0.0322150500891832	0.1898644188962221
7	0.034479366708870196	4.411006401987658E-5	0.0	7.351677336646098E-5	0.03500133579977207	0.1898938256055687
8	0.08036118496687848	5.146174135652268E-5	0.0	8.086845070310706E-5	0.03771410473699448	0.18991588063757864
9	0.1786531109578368	7.351677336646098E-5	0.0	8.086845070310706E-5	0.04040481864220695	0.18992323231491529
10	0.29146459968867117	8.822012803975316E-5	0.0	8.086845070310706E-5	0.04344106138224179	0.18995263902426185
11	0.531732118404939	9.557180537639926E-5	0.0	8.822012803975316E-5	0.0463302705755437	0.19000410076561838
12	0.6658487680573737	9.557180537639926E-5	0.0	8.822012803975316E-5	0.0496311736996978	0.19010702424833142
13	0.9123063990910975	9.557180537639926E-5	0.0	1.1027516004969146E-4	0.05302029695189165	0.19015848598968796
14	1.1139482050806266	9.557180537639926E-5	0.0	1.1027516004969146E-4	0.05749746844990913	0.19018054102169787
15	1.309039666563204	1.0292348271304536E-4	0.0	1.1762683738633756E-4	0.06223194865470921	0.19018054102169787
16	1.580816474344337	1.0292348271304536E-4	7.3516773366460975E-6	1.2497851472298365E-4	0.06615039267514158	0.19018789269903452
17	1.8466531268374597	1.0292348271304536E-4	1.4703354673292195E-5	1.2497851472298365E-4	0.07020851856497023	0.19022465108571776
18	2.153276885194295	1.0292348271304536E-4	1.4703354673292195E-5	1.3233019205962976E-4	0.07505327392982	0.19028346450441094
19	2.478507738890182	1.0292348271304536E-4	2.205503200993829E-5	1.3968186939627584E-4	0.08087580238044371	0.19032022289109415
20	2.8449520957353065	1.0292348271304536E-4	2.205503200993829E-5	1.5438522406956804E-4	0.08733057508201898	0.19033492624576745
21	3.2134475705573555	1.0292348271304536E-4	2.205503200993829E-5	1.6173690140621413E-4	0.09338835720741537	0.19036433295511404
22	3.59547748335617	1.0292348271304536E-4	2.205503200993829E-5	1.6173690140621413E-4	0.09829192599095832	0.19040844301913393
23	3.965406535258865	1.0292348271304536E-4	2.205503200993829E-5	1.6173690140621413E-4	0.10286466929435219	0.19043049805114384
24	4.338210093000189	1.0292348271304536E-4	2.205503200993829E-5	1.7644025607950633E-4	0.10687868512016097	0.19047460811516373
25	4.693832780805771	1.1027516004969146E-4	2.205503200993829E-5	1.7644025607950633E-4	0.11049571036979083	0.19048931146983702
26	5.06936381083899	1.1027516004969146E-4	2.205503200993829E-5	1.9849528808944464E-4	0.11453913290494619	0.19052606985652026
27	5.435536155622659	1.1027516004969146E-4	2.205503200993829E-5	2.0584696542609072E-4	0.11835465344266552	0.19057753159787677
28	5.808597022070765	1.2497851472298365E-4	2.205503200993829E-5	2.0584696542609072E-4	0.1217805350815426	0.1905995866298867
29	6.183966315202578	1.2497851472298365E-4	2.205503200993829E-5	2.131986427627368E-4	0.12488294291760725	0.19060693830722336
30	6.567672410434147	1.2497851472298365E-4	2.205503200993829E-5	2.2055032009938292E-4	0.12849261648990049	0.19063634501656995
31	6.963957225588719	1.2497851472298365E-4	2.940670934658439E-5	2.2055032009938292E-4	0.131764112904708	0.1906584000485799
32	7.373475059949253	1.2497851472298365E-4	2.940670934658439E-5	2.2055032009938292E-4	0.1349547408688124	0.1906804550805898
33	7.800791305141807	1.3233019205962976E-4	2.940670934658439E-5	2.2055032009938292E-4	0.13839532586236278	0.1907098617899364
34	8.236922211461	1.4703354673292196E-4	2.940670934658439E-5	2.2055032009938292E-4	0.14191677930661625	0.1907245651446097
35	8.679309395193679	1.4703354673292196E-4	2.940670934658439E-5	2.499570294459673E-4	0.1454161777188598	0.19077602688596623
36	9.13999490381727	1.5438522406956804E-4	2.940670934658439E-5	2.499570294459673E-4	0.14824657349346854	0.1908054335953128
37	9.60587804831787	1.5438522406956804E-4	2.940670934658439E-5	2.646603841192595E-4	0.15106961759074064	0.19081278527264944
38	10.078282130616074	1.5438522406956804E-4	2.940670934658439E-5	2.793637387925517E-4	0.15405439858941897	0.19084219198199603
39	10.558376067408412	1.5438522406956804E-4	2.940670934658439E-5	2.793637387925517E-4	0.1568039259133246	0.19086424701400598
40	11.045079162126394	1.5438522406956804E-4	2.940670934658439E-5	2.793637387925517E-4	0.15948728814120045	0.19087895036867927
41	11.533318757407734	1.5438522406956804E-4	2.940670934658439E-5	2.8671541612919777E-4	0.16222946378776942	0.19090100540068922
42	12.03169631573631	1.7644025607950633E-4	2.940670934658439E-5	3.0141877080249E-4	0.1647951991782589	0.19090100540068922
43	12.532007365204423	1.9114361075279852E-4	2.940670934658439E-5	3.2347380281242825E-4	0.16742709966477823	0.19090835707802586
44	13.037670435773615	1.9849528808944464E-4	2.940670934658439E-5	3.381771574857205E-4	0.17040452898611988	0.19091570875536248
45	13.542818888929242	2.0584696542609072E-4	2.940670934658439E-5	3.4552883482236657E-4	0.17327168314741187	0.19098187385139231
46	14.045776542238547	2.0584696542609072E-4	2.940670934658439E-5	3.4552883482236657E-4	0.17602856214865414	0.19098922552872896
47	14.546940386277711	2.2055032009938292E-4	2.940670934658439E-5	3.5288051215901265E-4	0.17854283579778712	0.19100392888340226
48	14.99057735515762	2.27901997436029E-4	2.940670934658439E-5	3.5288051215901265E-4	0.18135117654038593	0.19100392888340226
49	14.994981009882272	2.27901997436029E-4	2.940670934658439E-5	3.7493554416895096E-4	0.18438741928042077	0.19104068727008547
50	14.99831867139311	2.3525367477267512E-4	2.940670934658439E-5	3.8228722150559705E-4	0.1869310996389003	0.19108479733410536
51	15.000413899434053	2.573087067826134E-4	2.940670934658439E-5	3.969905761788893E-4	0.18974679205883577	0.19109950068877865
52	15.001413727551837	3.087704481391361E-4	2.940670934658439E-5	4.0434225351553536E-4	0.19221695564394886	0.19112890739812524
53	15.002045971802788	3.381771574857205E-4	2.940670934658439E-5	4.1169393085218144E-4	0.1947459326477551	0.19114361075279854
>>END_MODULE