##FastQC	0.12.1
>>Basic Statistics	pass
#Measure	Value
Filename	Trimmomatic on ERR109239_forward _R2 paired_.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1765231
Total Bases	129.2 Mbp
Sequences flagged as poor quality	0
Sequence length	1-75
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.006614431765584	34.0	31.0	34.0	31.0	34.0
2	33.21156506517564	34.0	34.0	34.0	31.0	34.0
3	33.259632167368665	34.0	34.0	34.0	31.0	34.0
4	36.50623992691845	37.0	37.0	37.0	35.0	37.0
5	36.48632417984752	37.0	37.0	37.0	35.0	37.0
6	36.50791232422647	37.0	37.0	37.0	35.0	37.0
7	36.51003015736248	37.0	37.0	37.0	35.0	37.0
8	36.50381999208562	37.0	37.0	37.0	35.0	37.0
9	38.354754043705526	39.0	39.0	39.0	37.0	39.0
10	38.340424663220425	39.0	39.0	39.0	37.0	39.0
11	38.32828069721728	39.0	39.0	39.0	37.0	39.0
12	38.24226462314802	39.0	39.0	39.0	37.0	39.0
13	38.28969252187884	39.0	39.0	39.0	37.0	39.0
14	39.91660902893424	41.0	40.0	41.0	38.0	41.0
15	39.90454713115917	41.0	40.0	41.0	38.0	41.0
16	39.888435676065896	41.0	40.0	41.0	38.0	41.0
17	39.86946129469321	41.0	40.0	41.0	38.0	41.0
18	39.83905982745868	41.0	40.0	41.0	38.0	41.0
19	39.82286760367923	41.0	40.0	41.0	38.0	41.0
20	39.82065644246687	41.0	40.0	41.0	38.0	41.0
21	39.80732385854384	41.0	40.0	41.0	38.0	41.0
22	39.768053213777684	41.0	40.0	41.0	38.0	41.0
23	39.502979755400254	41.0	40.0	41.0	37.0	41.0
24	39.619013581535846	41.0	40.0	41.0	37.0	41.0
25	39.618740365307715	41.0	40.0	41.0	37.0	41.0
26	39.585808000437105	41.0	39.0	41.0	37.0	41.0
27	39.48784613360625	41.0	39.0	41.0	37.0	41.0
28	39.492730979577	41.0	39.0	41.0	37.0	41.0
29	39.30197036662614	40.0	39.0	41.0	37.0	41.0
30	39.34966235693695	40.0	39.0	41.0	36.0	41.0
31	39.573712405907685	41.0	39.0	41.0	37.0	41.0
32	39.608877142857146	41.0	40.0	41.0	37.0	41.0
33	39.61962045398026	41.0	40.0	41.0	37.0	41.0
34	39.617063517030346	41.0	40.0	41.0	37.0	41.0
35	39.60709468642436	41.0	40.0	41.0	37.0	41.0
36	39.47718111312118	41.0	39.0	41.0	37.0	41.0
37	39.51376777628667	41.0	39.0	41.0	37.0	41.0
38	39.17258450433496	41.0	39.0	41.0	36.0	41.0
39	39.374163364523326	41.0	39.0	41.0	36.0	41.0
40	39.40676349761044	41.0	39.0	41.0	37.0	41.0
41	39.41248488799438	41.0	39.0	41.0	37.0	41.0
42	39.39732444088051	40.0	39.0	41.0	37.0	41.0
43	39.376534629130454	40.0	39.0	41.0	37.0	41.0
44	39.35366044708208	40.0	39.0	41.0	36.0	41.0
45	39.29479168767993	40.0	39.0	41.0	36.0	41.0
46	39.26615966072975	40.0	39.0	41.0	36.0	41.0
47	39.375739199336095	40.0	39.0	41.0	37.0	41.0
48	39.41218498071193	41.0	39.0	41.0	37.0	41.0
49	39.443958470934334	41.0	39.0	41.0	37.0	41.0
50	39.4247784080068	41.0	39.0	41.0	37.0	41.0
51	39.40655377090326	41.0	39.0	41.0	37.0	41.0
52	39.39592530713896	41.0	39.0	41.0	36.0	41.0
53	39.373748011590415	40.0	39.0	41.0	36.0	41.0
54	39.23241732056118	40.0	39.0	41.0	36.0	41.0
55	39.24856704267776	40.0	39.0	41.0	36.0	41.0
56	39.20654500737655	40.0	39.0	41.0	36.0	41.0
57	39.04173712372443	40.0	39.0	41.0	36.0	41.0
58	39.02812193833501	40.0	38.0	41.0	35.0	41.0
59	38.9267552031807	40.0	38.0	41.0	35.0	41.0
60	38.153831188861524	40.0	38.0	41.0	34.0	41.0
61	38.49194639742959	40.0	38.0	41.0	34.0	41.0
62	38.59503083475488	40.0	38.0	41.0	34.0	41.0
63	38.417696833748295	40.0	38.0	41.0	34.0	41.0
64	38.45514345383679	40.0	38.0	41.0	34.0	41.0
65	38.43146582970871	40.0	37.0	41.0	34.0	41.0
66	38.321080204653484	40.0	37.0	41.0	34.0	41.0
67	38.140256108817326	39.0	37.0	41.0	34.0	41.0
68	37.66231123995569	39.0	36.0	41.0	34.0	41.0
69	37.40427154387781	39.0	36.0	40.0	32.0	41.0
70	35.10896461722769	38.0	35.0	40.0	24.0	41.0
71	35.496447500581475	38.0	35.0	40.0	25.0	41.0
72	36.32280071041292	38.0	35.0	40.0	29.0	41.0
73	36.86765998059332	37.0	35.0	40.0	32.0	41.0
74	36.89857188511165	37.0	35.0	39.0	33.0	41.0
75	35.26402890318212	36.0	34.0	38.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	9.0
21	26.0
22	29.0
23	72.0
24	118.0
25	186.0
26	368.0
27	942.0
28	2430.0
29	5658.0
30	10279.0
31	15963.0
32	22193.0
33	28644.0
34	37911.0
35	52209.0
36	82037.0
37	162225.0
38	408075.0
39	792912.0
40	142945.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	19.292300823989052	40.38401832899734	21.777751842417757	18.545929004595845
2	16.291588065011357	37.371503996646325	31.926661114982185	14.41024682336013
3	15.327908089995212	32.86626765729759	35.82473423766645	15.981090015040747
4	16.780446548430948	32.73676233939838	34.71282570874734	15.769965403423331
5	16.896467297050375	34.03788649919407	33.02622719657589	16.039419007179664
6	16.48361676452567	34.2399957376211	32.28519622530073	16.99119127255249
7	16.491145526279986	34.05996326697202	33.39139948301664	16.057491723731353
8	15.712027702596213	33.54358974940444	34.227820744972156	16.516561803027194
9	16.201301039255142	33.019895479135236	34.23684856608419	16.541954915525437
10	16.316542177119175	34.0155307609778	33.563013335087454	16.10491372681557
11	16.35054371559597	34.08932883706	33.5803221119327	15.97980533541133
12	16.25831651924917	33.57029043505982	33.83978848572273	16.33160455996828
13	16.248151942913616	33.712021930466534	33.75428754917279	16.285538577447063
14	16.260025135536143	33.705505703724086	33.81376440115862	16.22070475958115
15	16.20380090908575	33.47501149125246	33.91905618513117	16.40213141453062
16	16.317591266228828	33.570092418336564	33.7250310444793	16.387285270955307
17	16.381052821580226	33.55211064383875	33.70093900364465	16.365897530936376
18	16.32609500280435	33.413849636796726	33.702800037239975	16.557255323158948
19	16.55540281262758	33.446442594494776	33.523552221330746	16.474602371546887
20	16.418841983013934	33.47436330646192	33.626562096254375	16.48023261426977
21	16.502310005327722	33.27594765881216	33.60714119375077	16.614601142109347
22	16.563009857433066	33.48595917959252	33.35064102855429	16.60038993442012
23	16.81664456176874	33.513393976363204	33.238357123812634	16.431604338055426
24	16.55291790869089	33.28854274591511	33.53596193236034	16.622577413033664
25	16.631398715563975	33.465758801344734	33.32252541283412	16.580317070257173
26	16.600635289952777	33.487270581043866	33.45551177494731	16.45658235405605
27	16.71852156862075	33.25325865771835	33.4123259187373	16.6158938549236
28	16.633845817222056	33.38416006545936	33.382963471990024	16.599030645328554
29	16.888033632641324	33.35936755664155	33.229006270463444	16.52359254025368
30	16.655135999086678	33.240345920027394	33.44595713103291	16.658560949853012
31	16.674795680010053	33.430462668410506	33.26906272666526	16.62567892491419
32	16.647866494779382	33.524535081463604	33.331390458427045	16.49620796532997
33	16.66177870391809	33.28794102429975	33.39261774702077	16.657662524761392
34	16.739382846684016	33.407612892155456	33.21503131524008	16.63797294592044
35	16.68905096122758	33.458330591328775	33.3399332190752	16.512685228368447
36	16.737420019505134	33.20453989704543	33.35618285804604	16.7018572254034
37	16.66538693828997	33.445662924129685	33.20213252408659	16.686817613493762
38	16.95078637372978	33.26472765246929	33.13401919887997	16.65046677492096
39	16.614105463437095	33.229072115348735	33.45585174697823	16.70097067423594
40	16.662405921306238	33.355316864451424	33.34779114343277	16.63448607080957
41	16.641113508336538	33.413643259513734	33.39777673150673	16.547466500642997
42	16.69073091264276	33.301215714201895	33.302596292457096	16.70545708069825
43	16.672029452920974	33.47932775411692	33.180009451551015	16.66863334141109
44	16.675382839660045	33.381118695845494	33.41043498694878	16.533063477545678
45	16.769999106635964	33.19296951323187	33.346628127134345	16.69040325299781
46	16.715565862805022	33.3329680552808	33.302573076277554	16.648893005636626
47	16.685085933816445	33.38910091084478	33.342066855303045	16.583746300035724
48	16.655829156175646	33.27672033448061	33.372929708253444	16.694520801090295
49	16.683231766159352	33.47353956496967	33.204433745187934	16.638794923683044
50	16.649494371303184	33.437812584921915	33.400056662792785	16.512636380982116
51	16.62951239282588	33.23223667957086	33.36721864822472	16.771032279378538
52	16.706379667869633	33.39857612102393	33.22056230367826	16.674481907428184
53	16.637933930927893	33.45550538912582	33.38095089135202	16.525609788594263
54	16.74118764327798	33.17590390504605	33.36653536596255	16.716373085713425
55	16.684264388167833	33.33557655937084	33.28197119233652	16.6981878601248
56	16.64196206232063	33.447827997893576	33.34889346015998	16.561316479625816
57	16.826511423779948	33.23466923208831	33.26512877002177	16.673690574109973
58	16.693448356911304	33.31097324002988	33.28392004821868	16.711658354840136
59	16.63211668455342	33.40785176018467	33.36164917027607	16.59838238498585
60	16.730794397109754	33.0446319656386	33.07752347390412	17.147050163347526
61	16.660681974223326	33.370723040520076	33.26188741214491	16.706707573111693
62	16.612352289782866	33.390136509157905	33.40184850417327	16.595662696885956
63	16.851071076243716	33.218924695823745	33.24347019307559	16.686534034856955
64	16.67386713172854	33.328807326723016	33.317698037770406	16.67962750377804
65	16.64713137133066	33.36651979890414	33.41318091095671	16.573167918808487
66	16.586205921376674	33.267798183947754	33.46169345811131	16.684302436564252
67	16.683309372558185	33.37706469881424	33.262572324950604	16.677053603676974
68	16.80187634203489	33.30791909609964	33.16095162770773	16.729252934157742
69	16.589195281675917	33.17970532586389	33.4581959306905	16.772903461769694
70	19.069033289809177	33.04924678974198	31.4536785917653	16.428041328683545
71	16.43797614082139	33.44962766323761	33.49828936887912	16.614106827061885
72	16.640627748582627	33.285229744045466	33.26978881764201	16.804353689729894
73	16.60141319428532	33.425385560020906	33.321644161690465	16.6515570840033
74	16.62325730036352	33.46189696267594	33.412628663497514	16.50221707346303
75	16.629773199028826	33.25756691505765	33.401591367815925	16.711068518097594
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	295.0
1	295.0
2	297.5
3	310.0
4	337.5
5	371.5
6	425.5
7	483.5
8	530.5
9	603.0
10	746.5
11	986.5
12	1173.5
13	1578.5
14	2550.0
15	4159.5
16	5295.0
17	7103.0
18	11349.5
19	17104.5
20	20880.5
21	26364.0
22	37533.0
23	50239.0
24	57696.0
25	66984.0
26	83059.5
27	99145.0
28	108733.5
29	117398.0
30	131435.0
31	141780.0
32	145777.5
33	147421.5
34	146614.5
35	139476.5
36	133959.0
37	126442.0
38	110665.0
39	94097.5
40	84823.0
41	74499.5
42	58819.5
43	44875.5
44	36560.0
45	31105.0
46	23148.0
47	16858.5
48	13897.5
49	11589.5
50	8375.0
51	6307.0
52	5278.5
53	4452.0
54	3486.5
55	2935.0
56	2633.5
57	2228.0
58	1760.5
59	1443.5
60	1241.0
61	1073.5
62	862.5
63	669.5
64	525.5
65	441.0
66	382.0
67	350.0
68	328.5
69	306.5
70	276.0
71	254.5
72	249.0
73	239.5
74	233.5
75	219.5
76	207.0
77	206.0
78	205.0
79	195.5
80	185.0
81	181.5
82	178.5
83	126.5
84	75.0
85	74.5
86	74.0
87	74.0
88	62.5
89	51.0
90	45.5
91	40.0
92	38.0
93	35.0
94	33.0
95	32.0
96	32.0
97	32.0
98	32.0
99	32.0
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009007319721894754
2	0.0
3	0.0
4	0.16258605330147727
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	5.676821933423368E-5
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	5.686457814444058E-4
25	0.0
26	6.26065734625533E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	5.711217974345209E-4
32	9.142857142857143E-4
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0010908268352587385
40	0.0
41	0.0
42	5.752406087656314E-5
43	0.0016116879610938526
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	5.7785197397123566E-5
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.10364919094606805
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-1	1.0
2-3	626.0
4-5	331.0
6-7	395.0
8-9	410.0
10-11	561.0
12-13	462.0
14-15	457.0
16-17	439.0
18-19	694.0
20-21	559.0
22-23	1732.0
24-25	1560.0
26-27	2022.0
28-29	3132.0
30-31	1850.0
32-33	1650.0
34-35	2143.0
36-37	3397.0
38-39	2113.0
40-41	2294.0
42-43	2167.0
44-45	2397.0
46-47	2199.0
48-49	2110.0
50-51	2136.0
52-53	2600.0
54-55	2443.0
56-57	3515.0
58-59	7148.0
60-61	4037.0
62-63	6372.0
64-65	3971.0
66-67	11901.0
68-69	143668.0
70-71	6857.0
72-73	32902.0
74-75	1501980.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.87754768320453
#Duplication Level	Percentage of total
1	82.69704196100048
2	14.752175686947227
3	2.1498621116718724
4	0.2922436498722246
5	0.045414948717066625
6	0.005864233726813697
7	0.004844301702250733
8	0.008750385487767711
9	0.003869340931065007
>10	0.039933379943229715
>50	0.0
>100	0.0
>500	0.0
>1k	0.0
>5k	0.0
>10k+	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	PolyA	PolyG
1	0.0	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	5.6649809571665124E-5	0.0
11	0.0	0.0	0.0	0.0	5.6649809571665124E-5	0.0
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>>END_MODULE