##FastQC	0.11.7
>>Basic Statistics	pass
#Measure	Value
Filename	rna fastq
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	99541
Sequences flagged as poor quality	0
Sequence length	75
%GC	52
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	9.0	9.0	9.0	9.0	9.0	9.0
2	9.0	9.0	9.0	9.0	9.0	9.0
3	9.0	9.0	9.0	9.0	9.0	9.0
4	9.0	9.0	9.0	9.0	9.0	9.0
5	9.0	9.0	9.0	9.0	9.0	9.0
6	9.0	9.0	9.0	9.0	9.0	9.0
7	9.0	9.0	9.0	9.0	9.0	9.0
8	9.0	9.0	9.0	9.0	9.0	9.0
9	9.0	9.0	9.0	9.0	9.0	9.0
10	9.0	9.0	9.0	9.0	9.0	9.0
11	9.0	9.0	9.0	9.0	9.0	9.0
12	9.0	9.0	9.0	9.0	9.0	9.0
13	9.0	9.0	9.0	9.0	9.0	9.0
14	9.0	9.0	9.0	9.0	9.0	9.0
15	9.0	9.0	9.0	9.0	9.0	9.0
16	9.0	9.0	9.0	9.0	9.0	9.0
17	9.0	9.0	9.0	9.0	9.0	9.0
18	9.0	9.0	9.0	9.0	9.0	9.0
19	9.0	9.0	9.0	9.0	9.0	9.0
20	9.0	9.0	9.0	9.0	9.0	9.0
21	9.0	9.0	9.0	9.0	9.0	9.0
22	9.0	9.0	9.0	9.0	9.0	9.0
23	9.0	9.0	9.0	9.0	9.0	9.0
24	9.0	9.0	9.0	9.0	9.0	9.0
25	9.0	9.0	9.0	9.0	9.0	9.0
26	9.0	9.0	9.0	9.0	9.0	9.0
27	9.0	9.0	9.0	9.0	9.0	9.0
28	9.0	9.0	9.0	9.0	9.0	9.0
29	9.0	9.0	9.0	9.0	9.0	9.0
30	9.0	9.0	9.0	9.0	9.0	9.0
31	9.0	9.0	9.0	9.0	9.0	9.0
32	9.0	9.0	9.0	9.0	9.0	9.0
33	9.0	9.0	9.0	9.0	9.0	9.0
34	9.0	9.0	9.0	9.0	9.0	9.0
35	9.0	9.0	9.0	9.0	9.0	9.0
36	9.0	9.0	9.0	9.0	9.0	9.0
37	9.0	9.0	9.0	9.0	9.0	9.0
38	9.0	9.0	9.0	9.0	9.0	9.0
39	9.0	9.0	9.0	9.0	9.0	9.0
40	9.0	9.0	9.0	9.0	9.0	9.0
41	9.0	9.0	9.0	9.0	9.0	9.0
42	9.0	9.0	9.0	9.0	9.0	9.0
43	9.0	9.0	9.0	9.0	9.0	9.0
44	9.0	9.0	9.0	9.0	9.0	9.0
45	9.0	9.0	9.0	9.0	9.0	9.0
46	9.0	9.0	9.0	9.0	9.0	9.0
47	9.0	9.0	9.0	9.0	9.0	9.0
48	9.0	9.0	9.0	9.0	9.0	9.0
49	9.0	9.0	9.0	9.0	9.0	9.0
50	9.0	9.0	9.0	9.0	9.0	9.0
51	9.0	9.0	9.0	9.0	9.0	9.0
52	9.0	9.0	9.0	9.0	9.0	9.0
53	9.0	9.0	9.0	9.0	9.0	9.0
54	9.0	9.0	9.0	9.0	9.0	9.0
55	9.0	9.0	9.0	9.0	9.0	9.0
56	9.0	9.0	9.0	9.0	9.0	9.0
57	9.0	9.0	9.0	9.0	9.0	9.0
58	9.0	9.0	9.0	9.0	9.0	9.0
59	9.0	9.0	9.0	9.0	9.0	9.0
60	9.0	9.0	9.0	9.0	9.0	9.0
61	9.0	9.0	9.0	9.0	9.0	9.0
62	9.0	9.0	9.0	9.0	9.0	9.0
63	9.0	9.0	9.0	9.0	9.0	9.0
64	9.0	9.0	9.0	9.0	9.0	9.0
65	9.0	9.0	9.0	9.0	9.0	9.0
66	9.0	9.0	9.0	9.0	9.0	9.0
67	9.0	9.0	9.0	9.0	9.0	9.0
68	9.0	9.0	9.0	9.0	9.0	9.0
69	9.0	9.0	9.0	9.0	9.0	9.0
70	9.0	9.0	9.0	9.0	9.0	9.0
71	9.0	9.0	9.0	9.0	9.0	9.0
72	9.0	9.0	9.0	9.0	9.0	9.0
73	9.0	9.0	9.0	9.0	9.0	9.0
74	9.0	9.0	9.0	9.0	9.0	9.0
75	9.0	9.0	9.0	9.0	9.0	9.0
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
9	99541.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	26.951708341286505	23.45465687505651	23.515938156136666	26.077696627520318
2	25.593474045870547	23.638500718296985	23.793210837745253	26.974814398087222
3	26.41122753438282	24.344742367466672	23.735948001326086	25.508082096824424
4	25.87275594980963	23.006600295355682	25.295104529791747	25.82553922504295
5	26.092765794999046	23.40141248329834	25.214735636571866	25.291086085130747
6	25.733114997840083	23.138204358003232	23.876593564460876	27.2520870796958
7	25.903898895932333	24.442189650495777	23.793210837745253	25.860700615826644
8	27.217930300077352	23.370269537175638	23.84745984066867	25.564340322078337
9	26.077696627520318	23.183411860439417	24.746586833566067	25.9923046784742
10	25.904903507097575	23.258757697833055	24.7596467787143	26.076692016355068
11	25.70297666288263	23.62744999547925	25.06203473945409	25.607538602184025
12	26.897459338363088	23.475753709526728	23.909745732914075	25.717041219196112
13	26.07267357169408	24.222179805306357	23.85348750766016	25.85165911533941
14	26.185190022201905	24.416069760199317	23.63548688480124	25.763253332797543
15	26.312775640188462	23.27382686531178	23.66562521975869	26.74777227474106
16	26.75580916406305	23.182407249274167	23.97805929215097	26.08372429451181
17	26.192222300358647	23.332094312896192	23.80225233823249	26.673431048512676
18	27.13454757336173	23.325062034739457	23.91979184456656	25.620598547332257
19	26.020433791101155	23.530002712450145	23.735948001326086	26.71361549512261
20	26.035502958579883	23.344149646879174	25.039933293818628	25.580414100722315
21	26.130941019278488	24.41908359369506	23.53402115711114	25.91595422991531
22	26.34190936398067	23.410453983785576	23.458675319717504	26.788961332516248
23	26.29971569504023	23.208527139570627	25.337298198732185	25.154458966656957
24	25.870746727479126	23.271817642981286	23.75302639113531	27.104409238404276
25	26.21633296832461	24.67726866316392	23.684712831898413	25.421685536613055
26	25.917963452245807	24.55169226750786	23.68873127655941	25.841613003686923
27	25.804442390572728	23.523975045458656	23.872575119799883	26.799007444168733
28	27.321405250097953	22.97545734923298	23.622426939653007	26.080710461016064
29	27.31537758310646	23.505892044484185	23.554113380416112	25.62461699199325
30	25.969198621673485	24.73453149958309	23.757044835796304	25.539225042947127
31	27.436935534101526	23.111079856541526	23.599320882852293	25.852663726504655
32	25.985272400317456	22.92321756864006	23.838418340181434	27.253091690861055
33	26.232406746968586	23.171356526456435	23.632473051305492	26.963763675269487
34	27.298299193297233	23.187430305100413	23.997146904290695	25.51712359731166
35	25.80142855707698	23.442601541073525	24.548678434012118	26.207291467837372
36	27.436935534101526	23.054821631287613	23.925819511558053	25.58242332305281
37	26.391135311077846	23.66662983092394	24.247295084437567	25.694939773560645
38	25.867732893983387	23.476758320691975	23.26076692016355	27.394741865161087
39	25.9902954561437	23.949930179524014	23.171356526456435	26.88841783787585
40	25.969198621673485	24.22619824996735	23.710832722194873	26.093770406164296
41	25.66379682743794	24.674254829668175	23.628454606644496	26.033493736249387
42	27.144593685014218	23.385338704654362	23.40944937262033	26.060618237711093
43	27.821701610391695	22.965411237580494	23.37428798183663	25.83859917019118
44	25.857686782330898	24.423102038356053	23.469726042535235	26.24948513677781
45	25.917963452245807	23.320038978913214	24.68430094132066	26.077696627520318
46	26.23943902512533	23.6595975527672	24.44721270632202	25.653750715785456
47	26.252498970273557	23.315015923086968	23.326066645904703	27.106418460734773
48	25.653750715785456	23.527993490119652	24.879195507378867	25.939060286716025
49	25.90992656292382	23.502878210988438	23.44159692990828	27.145598296179465
50	27.365608141368885	22.900111511839345	23.38232487115862	26.351955475633154
51	25.665806049768435	24.8591032840739	23.63950532946223	25.83558533669543
52	25.68489366190816	23.62041771732251	24.934449121467537	25.760239499301797
53	25.919972674576304	23.031715574486896	24.6230196602405	26.425292090696296
54	26.111853407138767	23.58123788187782	23.206517917240134	27.100390793743284
55	25.9571432876905	23.66662983092394	24.602927436935534	25.773299444450025
56	25.955134065360003	23.289900643955757	23.315015923086968	27.439949367597272
57	26.376066143599118	23.48780904350971	24.276428808229774	25.859696004661398
58	25.993309289639445	25.179574245788167	23.202499472579138	25.62461699199325
59	25.878783616801115	23.369264926010388	24.47333259661848	26.27861886057002
60	25.797410112415992	23.93988406787153	24.546669211681618	25.716036608030862
61	26.102811906651528	23.337117368722435	23.18039802694367	27.379672697682363
62	26.583016043640313	23.14624124732522	24.562742990325596	25.707999718708873
63	25.80946544639897	23.758049446961554	23.40040787213309	27.032077234506385
64	27.16267668598869	23.895681176600597	23.119116745863515	25.822525391547202
65	26.120894907626003	25.00979495886117	23.05381702012236	25.815493113390463
66	25.93604645322028	23.471735264865735	24.786771280176005	25.80544700173798
67	25.787364000763503	24.06746968585809	24.35177464562341	25.793391667754996
68	26.06463668237209	23.40040787213309	23.205513306074884	27.329442139419935
69	25.798414723581235	23.319034367747964	23.74800333530907	27.13454757336173
70	26.211309912498365	22.864950121055646	23.530002712450145	27.39373725399584
71	26.105825740147274	23.731929556665094	23.192453360926653	26.96979134226098
72	25.947097176038014	24.497443264584444	23.975045458655227	25.580414100722315
73	25.955134065360003	23.803256949397735	24.55269687867311	25.688912106569152
74	26.453421203323252	23.317025145417468	23.30195597793874	26.92759767332054
75	25.861705226991894	23.559136436242355	24.34775620096242	26.23140213580334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	2.0
6	3.5
7	20.5
8	37.0
9	20.5
10	9.0
11	21.5
12	29.0
13	31.5
14	210.5
15	250.0
16	113.0
17	176.0
18	223.0
19	246.0
20	285.0
21	231.5
22	246.0
23	328.5
24	343.0
25	383.5
26	446.5
27	551.0
28	633.0
29	632.0
30	674.5
31	771.5
32	825.0
33	822.5
34	791.0
35	949.0
36	1136.0
37	1084.0
38	1097.5
39	1130.5
40	1098.0
41	1098.5
42	1246.0
43	1692.0
44	1991.0
45	2361.0
46	2966.0
47	3640.5
48	4080.0
49	4876.0
50	5369.0
51	5207.0
52	5348.0
53	5632.0
54	6445.0
55	7429.5
56	7885.0
57	7176.0
58	6285.5
59	5676.0
60	5248.0
61	4706.0
62	3782.5
63	3405.5
64	3410.0
65	3140.0
66	2352.0
67	1609.0
68	1384.0
69	1049.0
70	489.5
71	197.0
72	129.0
73	100.0
74	73.5
75	66.0
76	56.0
77	48.0
78	33.5
79	21.5
80	16.0
81	9.0
82	3.0
83	2.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	99541.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.00086396560211
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.81530333190706	40.94091881737174
2	11.45424441426525	12.370781888869912
3	4.07419120793258	6.600295355682582
4	2.3514966606514984	5.079314051496368
5	1.4566628839320594	3.9330527119478402
6	0.7980949900470671	2.5858691393496147
7	0.427883094897029	1.6174239760500697
8	0.3683515338678771	1.5913040857536092
9	0.32928394694249624	1.6003455862408453
>10	2.913325767864119	21.60617233099929
>50	0.005581083846482987	0.1918807325624617
>100	0.003720722564321991	0.6680664248902463
>500	0.0	0.0
>1k	0.0018603612821609956	1.214574898785425
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCATTCAGTTTGAACGCGCTACTCAACGGAGCCGTGTCCAAGGATTCTCGATTCTCTATTCTCGATTCCCCATCT	1209	1.214574898785425	No Hit
CAGTTCCAGCTACAGCGCAACGGAGATCGAGCGTCTGAACCCGCCGGAAGATCAACGACAATAAATAATTGTAGC	383	0.3847660762901719	No Hit
AATTTTTTCAATTTTTTTTTTATTTTATTTTTAAATAAATTAGAAAATTCTTCGTAAAATCGAAGAACCCATTTA	282	0.2833003486000743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.0	0.0
32	0.0	0.0	0.0	0.0	0.0
33	0.0	0.0	0.0	0.0	0.0
34	0.0	0.0	0.0	0.0	0.0
35	0.0	0.0	0.0	0.0	0.0
36	0.0	0.0	0.0	0.0	0.0
37	0.0	0.0	0.0	0.0	0.0
38	0.0	0.0	0.0	0.0	0.0
39	0.0	0.0	0.0	0.0	0.0
40	0.0	0.0	0.0	0.0	0.0
41	0.0	0.0	0.0	0.0	0.0
42	0.0	0.0	0.0	0.0	0.0
43	0.0	0.0	0.0	0.0	0.0
44	0.0	0.0	0.0	0.0	0.0
45	0.0	0.0	0.0	0.0	0.0
46	0.0	0.0	0.0	0.0	0.0
47	0.0	0.0	0.0	0.0	0.0
48	0.0	0.0	0.0	0.0	0.0
49	0.0	0.0	0.0	0.0	0.0
50	0.0	0.0	0.0	0.0	0.0
51	0.0	0.0	0.0	0.0	0.0
52	0.0	0.0	0.0	0.0	0.0
53	0.0	0.0	0.0	0.0	0.0
54	0.0	0.0	0.0	0.0	0.0
55	0.0	0.0	0.0	0.0	0.0
56	0.0	0.0	0.0	0.0	0.0
57	0.0	0.0	0.0	0.0	0.0
58	0.0	0.0	0.0	0.0	0.0
59	0.0	0.0	0.0	0.0	0.0
60	0.0	0.0	0.0	0.0	0.0
61	0.0	0.0	0.0	0.0	0.0
62	0.0	0.0	0.0	0.0	0.0
63	0.0	0.0	0.0	0.0	0.0
>>END_MODULE