FastQCFastQC Report
Tue 10 May 2016
SRR192336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR192336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30246
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCNNATGCCGTCTTCT620.20498578324406533Illumina Paired End PCR Primer 2 (97% over 42bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATATCGTATGCCGTCTTCT510.16861733782979568Illumina Paired End PCR Primer 2 (98% over 56bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGNNACCGATATCGTA500.16531111551940753Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC100.0014437551245.1249861
AAAACCT100.0030703717190.7999963
CAAGTCC150.004657691165.718358
CTTGCTA150.004858103163.4166759
TCGTATG150.004858103163.4166760
GTATGCC150.005064165161.1780962
GGACACT150.005064165161.1780962
GCCGTCT201.7788901E-594.12866
ATGCCGT255.599522E-574.70476564
TATCGTA200.002631518366.8522769
CCGTCTT301.3099615E-462.9197967
TGCCGTC301.3811399E-462.2539765
AGACCGA400.00320730760.4417862
CGTCTTC301.6339881E-460.18414368
CGGAAGC150.004506254658.44043
CGCAGGG150.004506254658.44041
GTCTTCT450.001285661539.6161669
GACCGAT502.7770334E-438.1663
CGATATC350.00185784536.8070955
CGAGATC1005.4569682E-1132.356518
Produced by FastQC (version 0.11.4)